French Flag   chineseFlag   japaneseFlag   germanFlag   SpanishFlag   argentinaFlag   mexicoFlag   russianFlag

View Cart || Request a 2017 Catalog

Electron Microscopy Sciences

EMS Academy Applications

arrow13The Biological SEM Workshop: A Complete Picture

This course will introduce participants to methods of sample preparation and SEM parameters and operation needed for accurate analysis.

Examples of the endless possibilities in the field of Microscopy: SEM

Mouse Lung Tissue
Mouse Lung Tissue: Red blood cells in the lung.
Louisa Howard, Dartmouth College
  Mouse Lung Tissue
Mouse Lung Tissue: Tracchea area. Red blood cells inside blood vessel in the lung.
Louisa Howard, Dartmouth College
Human Blood Cells
Human Blood Cells: Red blood cells/Platelets/White blood cells.
Louisa Howard, Dartmouth College
  Human Blood Cells
Human Blood Cells: Red blood cells/Platelets.
Louisa Howard, Dartmouth College
Nicotiana alata stem cross section
Scanning Electron Microscope image of Nicotiana alata stem cross section. Image shows outer epidermal layer, followed by the cortex and then large vascular bundles. The vascular bundles contain the phloem (nearest the cortex) and xylem.
Louisa Howard, Dartmouth College
  sunflower lower leaf surface
Scanning Electron Microscope image of sunflower lower leaf surface.
Louisa Howard, Dartmouth College
Knot of human hair
Knot of human hair.
Courtesy of Frans Holthuysen
  Aphid on white poplar
Aphid on white poplar.
Courtesy of Riccardo Antonelli

Targeted Participants

Individuals who currently perform or will be responsible for the preparation of samples and /or operation of the SEM in a research, academic, or industrial setting.

Facility

The EMS Microscopy Academy
Located in Hatfield, Pennsylvania, the Academy provides electron microscopy classes, workshops and training sessions for all fields of microscopy, including materials science and biological science.

Scope of Class

The preparation of samples will start with determination of ROI and subsequent selection of gross cut orientation and determination of which dehydration technique to use: freeze drying, HMDS, Hitachi’s ionic liquid or critical point drying (CPD). The chemical processing required before CPD will be stressed. Special attention will be paid to orientation, stability and grounding of the sample when mounting to facilitate ease of imaging. The advantages, disadvantages and instrument requirements for the various coating materials: Au, Pt, Pt/Pd will be discussed.

Selection of accelerating voltage (kV) and spot size are critical for surface detail, resolution, and charging and will be covered in detail. Parameters such as working distance for depth of field (Dfi) and resolution, plus tilt and raster rotate will be examined for proper image collection.

Format

Lecture, demonstration and hands-on practice, as well as round table discussion. Participants are encouraged to bring their own samples, if possible.

Main Curriculum

  • Identification and isolation of ROI
  • Theory and hands-on chemical processing
  • Theory and hands-on CPD, HMDS, and Hitachi's ionic liquid
  • Theory and hands-on freeze drying
  • Mounting
  • Theory and hands-on Sputter coating
  • SEM theory and discussion of parameters affecting image quality
  • Demonstration and hands-on practice of SEM operation and effects of  parameters
  • Theory and demonstration and hands-on practice of variable pressure and Hitachi's ionic liquid

Equipment

Hitachi S3500 SEM EMS 850 Critical Point Dryer
PP3010T Cryo Preparation System TES 150T ES Sputter Coater

Faculty

Michael Kostrna
Michael was the program director of the Electron Microscopy Technician program at Madison Area Technical College and has more than 35 years in EM technical education and research experience. He has been training EM students for 29 years and has developed curricula and lab exercises for TEM, SEM, OLM, lab safety, introductory and advanced biological EM, EM, maintenance, and x-Ray microanalysis. He has worked with companies such as SC Johnson Polymer, Dow Chemicals, Io Genetics, Virent Technologies, ABS Global, NanoOnocology, and Microscopy Inovations, and in the process gained insight to the various applications of EM.

Al Coritz
Al has been doing Electron Microscopy for 38 years, beginning at the Yale School of Medicine and ending up on the commercial side with several key EM companies. His specialty is Cryo-techniques and Thin Film Technology: i.e. Freeze Fracture/Rotary Shadowing, High Pressure Freezing, and more. He is currently with Electron Microscopy Sciences where he has been the Technical Director for over 20 years.

View our schedule to see if this course is currently offered.

Return to Courses Page arrow13arrow13