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Electron Microscopy Sciences

EMS Academy Applications

arrow13Automated and Rapid Specimen Processing for Electron Microscopy Workshop: A Complete Picture

This class will introduce participants to the use of equipment for processing tissue samples for TEM.

Examples of the endless possibilities in the field of Microscopy

 
Eight mPrep/s capsules containing glutaraldehyde-fixed kidney specimens were mounted on the ASP-1000 before initiating the 45-minute processing program. Rapid processing, achieved by fluid exchanges occurring every half second, constantly delivered fresh reagent to the specimens.
-Microscopy Innovations Application Note #601
 
Cascade Hops Leaf
-Michael Kostrna, MATC
Neuron Stem Cells
-Michael Kostrna, MATC

Targeted Participants

Lab managers or technicians who are responsible for the processing of samples for TEM analysis and currently do not incorporate either a microwave or automated processor in their lab.

Scope of Class

The preparation of samples for EM requires many steps, with extended wait times in between, requiring an entire day of a technician’s time. This process consists of several fixative, wash, dehydration, and resin infiltration steps, which are tedious and prone to temporal variability between runs.

With the use of an automated tissue processor or microwave, a technician’s time is reduced and better continuity between processing runs is obtained.

This class will introduce participants to the use of a dedicated lab microwave, EMS 9000, and 2 models of automated tissue processors Lynx II, and Microscopy Innovation’s ASP 1000 for processing tissue samples for TEM.

Facility

The EMS Microscopy Academy
Located in Hatfield, Pennsylvania, the Academy provides electron microscopy classes, workshops and training sessions for all fields of microscopy, including materials science and biological science.

Format

Lecture, demonstration, and hands on operation of systems used. Discussions about advantages and disadvantages, along with participant questions, are a significant component of this workshop.

Main Curriculum

  • EM sample preparation requirements / time table
  • Microwave theory and processing
  • Lynx II setup and operation
  • Microscopy Innovations ASP 1000 setup and operation

Instruments Available

EMS 9000 Microwave Lynx II Processor Microscopy Innovations ASP 1000
RMC Ultramicrotome Leica Ultramicrotome Zeiss TEM

Faculty

Michael Kostrna
Michael was the program director of the Electron Microscopy Technician program at Madison Area Technical College and has more than 35 years in EM technical education and research experience. He holds and A.S. in Biological Electron Microscopy from San Joaquin Delta College and an M.A in Education in Math, Science, and Technology. He has been training EM students for 29 years and has developed curricula and lab exercises for TEM, SEM, OLM lab safety, introductory and advanced biological EM, EM, maintenance, and x-Ray microanalysis. He has worked with companies such as SC Johnson Polymer, and Dow Chemicals, to Genetics, Virent Technologies, ABS Global, NanoOnocology, and Microscopy Innovations, and in the process gained insight to the various applications of EM in industry.

Al Coritz
Al has been working in the Electron Microscopy field for 39 years, beginning at the Yale School of Medicine and ending up on the commercial side with several key EM companies. His specialty is Cryo-techniques and Thin Film Technology: i.e. Freeze Fracture/Rotary Shadowing, High Pressure Freezing, and more He is currently with Electron Microscopy Sciences where he has been the Technical Director for over 20 years.

View our schedule to see if this course is currently offered.

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