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Electron Microscopy Sciences

Chemicals for Microscopy

Certificates of Analysis

About Our Chemicals...

  • Storage conditions of EACH chemical is noted in the box left of the catalog number
  • CODE: = Room Temperature
  • For increased shelf life of room temperature stored chemicals, see equipment section for our complete line of Desiccator Cabinets. All chemicals must be kept away from direct sunlight
  • All specific gravities, unless otherwise specified are measured at water = 1
  • All of the EMS stains are certified
  • For a chart of uses and applications of our Dyes & Stains, see pages 326-327
  • EM Grade = ACS Reagent

arrow13Acetamido to Butyl

arrowmsds4-Acetamido-4'-Isothiocyanostilbene-2,2'-Disulfonic Acid (SITS), Sodium Salt

C17H12N2O7S3Na2   F.W. 498.50   CAS #5-1023-76-8 SITS has been used as a vital stain, as a label for immunostaining, and as a neuronal tracer. SITS is also a non-penetrating fluorescent marker for erythrocyte membranes which acts as a potent inhibitor of anion transport. For more information related to the preparation and characterization of SITS, please refer to: Jakobsen, P. and Horobin, Richard W.(1989). Stain Technology, Vol.64, No.6 pg.301-313.

RT 10030 SITS, Disodium Salt 250mg 320.00 Add to Cart

msdsarrowAcetate Buffer

Please see listing in Prepared Buffers.

RT 11480   225 ml 16.00 Add to Cart
RT 11482   500 ml 21.00 Add to Cart

msdsarrowAcetic Acid, Glacial, Reagent A.C.S

C2H4O2 F.W. 60.05 CAS 64-19-7

Assay min   99.7%
RT 10040 Acetic Acid, Glacial 500 ml 58.00 Add to Cart
RT 10041 Acetic Acid, Glacial 2.5 L 115.00 Add to Cart

arrowAcetic Acid, Aqueous Solution

Prepared from acetic acid glacial with de-ionized water

RT 10042-02 Acetic Acid, 0.2% (v/v) Solution msds 500 ml 18.00 Add to Cart
RT 10042-05 Acetic Acid, 0.5% (v/v) Solution msds 500 ml 19.00 Add to Cart
RT 10042-10 Acetic Acid, 1.0% (v/v) Solution msds 500 ml 21.00 Add to Cart
RT 10042-50 Acetic Acid, 5.0% (v/v) Solution msds 500 ml 29.00 Add to Cart

msdsarrowAcetone, Reagent, A.C.S.

CH3COCH3   F.W. 58.08   CAS #67-64-1 A.C.S. Specifications:

Assay   Min 99.5%
Aldehyde (as HCHO)   0.002%
Color   Max. APHA 10
Density (G/ml) at 25°C   Max. 0.7857
Titratable Acid   0.0003 meq/g
Titratable Base   0.0006 meq/g
Isopropyl Alchohol   0.05%
Methanol (CH3OH)   0.05%
Residue after Evaporation   0.001%
Solubilty in water   To pass test
Substances Reducing Permanganate   To pass test
Water   0.5%
RT 10000   1qt. (946ml) 13.00 Add to Cart
RT 10010   4x1 qt. 48.00 Add to Cart
RT 10012   250 ml 7.00 Add to Cart
RT 10014   4x250 ml 20.00 Add to Cart

msdsarrowAcetone, Glass Distilled CAS #67-64-1

U.V. Absorption - 1cm cell vs. H2O

Wavelength (nm) 400 350 340 330
Adsorption 0.01 0.02 0.10 1.0
RT 10015   100 ml 14.00 Add to Cart
RT 10016   4x100 ml 46.00 Add to Cart

arrowAcetone: Alcohol

Please see listing in Reagents for Histology: Fixing, Dehydrating, Clearing and Staining.

msdsarrowAcetonitrile, Reagent, A.C.S.

CH3CN   FW 41.05   CAS #75-05-8 A non-carcinogenic substitute for Propylene Oxide, used in tissueprocessing for TEM. Good for infiltration with EMbed-812 or other Epon-812 substitutes. Tarnowski, sB.I. et al, EMSA proceedings, Detroit, MI page 38 (1984)

RT 10020   450 ml 42.00 Add to Cart
RT 10021   4x450 ml 158.00 Add to Cart

Technical Tip

Use of Acetonitrile

arrowAcid Alcohols

An acidified alcohol solution for the differentiation of regressive stains.

RT 26071-01 Acid Alcohol, 0.5% HCl in 70% Alcohol msds 1 L 36.00 Add to Cart
RT 26071-04 Acid Alcohol, 0.5% HCl in 70% Alcohol 4x1 L 128.00 Add to Cart
RT 26072-01 Acid Alcohol, 1% HCl in 70% Alcohol msds 1 L 32.00 Add to Cart
RT 26072-04 Acid Alcohol, 1% HCl in 70% Alcohol 4x1 L 112.00 Add to Cart
RT 26073-01 Acid Alcohol, 1% HCl in 95% Alcohol msds 1 L 42.00 Add to Cart
RT 26073-04 Acid Alcohol, 1% HCl in 95% Alcohol 4x1 L 142.00 Add to Cart

msdsarrowAcid Fuchsin, Certified, C.N. #DcR-8

(Rubin S, Fuchsin S, Fuchsin Acid, Acid Magenta, Acid Violet 19) C20H17N3Na2O9S3   F.W. 585.54   CAS #3244-88-0 Solubility: 0.053% Water; 1.21% Alcohol

RT 10035   25 g 30.00 Add to Cart

msdstechnical data sheetarrowAcridine Orange

(Basic Orange 14) C17H2ON3 Cl   F.W. 301.82   CAS #65-61-2

Ultrastructural probes on DNA templates within human bone marrow and lymph node cells. Frenstar, J., Adv. Cell Mol. Biol., 3, 1(1974). Used to detect changes in DNA template activity within cells. Frenster, J. Cell Nucl., 1, 565 (1974) Fluorescent dye for cytochemistry. J. Histochem. Cytochem., 31, 737 ((1983) J. Lab. Med., 15 (3),180, (1984)

RT 10050   25g 62.00 Add to Cart

arrow14 Adhesives and Mountants

Please see the Adhesive and Mountants section.

msdsarrowAgar, Powder, Bacteriological Grade

(C12H18O9)x   F.W. 3000-9000   CAS #9002-18-0

Geling Temperature (1.5% in H2O)   ~35°C
Gel Strength (1.5% gel)   300g/cm2

Specifications:

Acid insoluble ash   0.5%
Arsenic   3ppm max
Gelatin   To Past Test
Heavy Metals   <0.004%
Lead   <0.001%
Total ash   <6.5%
Water   <20%
RT 10200   100g 27.00 Add to Cart
RT 10202   500g 118.00 Add to Cart

msdsmsdsarrowAgarose I™

(C6H10O5·C6H8O4)n   CAS # 9012-36-6

  • All purpose, high-purity Agarose
  • Exceptional Band Resolution & Clarity
  • Nuclease & Protease free
  • Convenient Tablet Form is available - no weighing required

AgaroseI™ is standard melting/gelling agarose, suitable for routine nucleic acid analytical/preservative applications. Agarose I™ has a low EEO for shorterelectrophoresis runs without compromising resolution. Excellent for blotting technique and general manipulations.

Biotechnology Grade:

Gel Strength (1.5%)   ≥ 1200 g/cm2
Gelling Range (1.5%)   36-39°C
Melting Point   87-89°C
EEO   ≤ 0.10 mr
Sulfate   ≤ 0.15 %
DNase   None Detected
RNase   None Detected
Protease   None Detected
pH (3%, Water) @ 25°C   5.7 – 7.7
Conductivity (3%, Water) @ 25°C   ≤ 17.0 µohms
Moisture (KF)   ≤ 10 %
Endonuclease/Ligase Inhibitory Factors   None Detected
RT 10205 Agarose I™ Powder  100g 180.00 Add to Cart
RT 10206 Agarose I™ 500 mg/Tablet  100 Tabs 160.00 Add to Cart

™ Trademark of Amresco®

Technical Data Sheet

Agarose for the Separation of Biological Molecules by Gel Electrophoresis

Agarose

msdsarrowAgarose SFR™

(C6H10O5·C6H8O4)n CAS #9012-36-6

  • Super  fine resolution: Resolve 238 bp & 242 bp band
  • Low melting point
  • Consistent & reproducible results
  • Nuclease & protease free
  • Ideal for AFLP’s, STR’s & Tetranucleotide repeats

Agarose SFR™ is a high resolution sieving agarose with unsurpassed clarity. DNA bands differing in size by 2% can be resolved in the range of 200 – 1,000 bp. This agarose is suitable for the analysis of AFLP’s (Amplified Fragment Length Polymorphisms), STR’s (Short Tandem Repeats) and tetranucleotide repeats. The low melting point of agarose SFR™ makes it an excellent medium for analytical and preparative electrophoresis.

Biotechnology Grade

Gel Strength (1.5%)   ≥ 500 g/cm2
Gelling Range (1.5%)   ≤ 30°C
Melting Point   ≤ 70°C
EEO   ≤ 0.12 mr
Sulfate   ≤ 0.11 %
Moisture (KF)   ≤ 7.0 %
Ash   ≤ 0.35 %
DNase   None Detected
RNase   None Detected
Protease   None Detected
pH (3%, Water) @ 25°C   5.7 – 7.7
Conductivity (3%, Water) @ 25°C   ≤ 10.0 µohms
RT 10207 Agarose Super Fine Resolution 100g 485.00 Add to Cart

™ trademark of Amresco®

msdsAgarose LE PowderarrowAgarose LE Powder

Features

  • Multi-purpose, high purity
  • Green Choice, free from organic solvents
  • Enhanced resolution and clarity
  • Low EEO / increase electrophoretic mobility
  • RNase, DNase and Protease free

EMS Agarose LE is a highly purified agarose, suitable for a variety of molecular biology applications. It is refined using an advanced process that excludes the use of organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact. EMS Agarose LE can be used for analyses of proteins and nucleic acids of various sizes (150 bp to 6 kbp).

It's low EEO (≤ 0.13 -mr) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.

EMS Agarose LE is widely used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion) and various blotting protocols. It is easily soluble, free of nucleases, and easy to use. It is highly transparent (forms a clear, colorless solution at 1g:100ml H2O), and exhibits exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio.

Formulated for high gel strength and integrity, EMS Agarose LE exhibits exceptional thermal stability and mechanical resistance, ensuring safe, easy handling, regardless of whether a denaturing agent has been added.

Specifications

Moisture ≤ 7%
EEO (Electroendosmosis) ≤ 0.13 -mr
Sulfate ≤ 0.20%
Gel Temperature (at 1.5%) 36°C ± 1.5°C
Melt Temperature (at 1.5%) 88°C ± 1.5°C
Gel Strength (at 1.0%) 1200g / cm2
Gel Strength (at 1.5%) 2500g / cm2
DNase, RNase None Detected
Endonuclease None Detected
Protease None Detected

Ordering Information

87046-01 Agarose LE Powder, 25g each 42.50 Add to Cart
87046-02 Agarose LE Powder, 100g each 104.00 Add to Cart
87046-03 Agarose LE Powder, 500g each 369.00 Add to Cart

EZ Pack™ Agarose TabletsarrowEZ Pack™ Agarose Tablets

Features

  • Eliminates weighing
  • Fast dissolving, just two minutes
  • Environmentally friendly, free from organic solvents
  • Consistent, reproducible gels
  • Enhanced resolution and clarity

Researchers trust EMS Agarose LE to meet their electrophoretic needs for a variety of applications. Now the same EMS agarose is available in a convenient, no mess tablet.

EMS Agarose LE is refined in an advanced process that excludes the use of organic solvents. The result is a cleaner end product with significantly reduced environmental impact. The EMS agarose can be used for analyses of nucleic acids from 150 bp to 6 kbp, protein electrophoresis and various blotting protocols.

The low EEO of the EMS agarose promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO reduces band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.

The quick dissolving EMS EZ Pack™ tablet contains 0.5g (500 mg) of EMS Agarose LE, eliminating the hands-on time and inaccuracies normally associated with weighing. Use of the tablet is not only convenient and cleaner, it also provides better consistency and reproducibilty gel to gel. Gel preparation is simple – add the desired number of tablets to electrophoresis buffer, allow to sit for two minutes and then heat and pour as usual. The resulting gels are highly transparent, have exceptional thermal stability (ensuring safe and easy handling) and exhibit exceptionally low absorption of chemical staining agents.

EMS EZ Pack™ Agarose Tablets are supplied in convenient blister packs for safe, clean dispensing.

Specifications


Gel Strength
≥ 1200g / cm2 (1%)
≥ 2500g / cm2 (1.5%)
Gel Temperature (at 1.5%) 36°C ± 1.5°C
Melt Temperature (at 1.5%) 88°C ± 1.5°C
EEO (electroendosmosis) ≤ 0.13 -mr
Moisture Content ≤ 10%
Sulfate ≤ 0.20%
DNase, RNase None Detected
Endonuclease None Detected
Protease None Detected
Storage Room Temperature

EZ Chart

Buffer volume required to achieve desired gel strength
Gel % 1 Tab 2 Tabs 3 Tabs
0.8% 63 ml 125 ml 188 ml
1.0% 50 ml 100 ml 150 ml
1.2% 42 ml 83 ml 125 ml
1.3% 38 ml 77 ml 115 ml
1.5% 33 ml 67 ml 100 ml
1.8% 28 ml 56 ml 83 ml
2.0% 25 ml 50 ml 75 ml

Ordering Information

87045-01 EZ Pack™ Agarose Tablets, 200/pk (100gm) each 145.00 Add to Cart
87045-02 EZ Pack™ Agarose Tablets, 1000/pk (500gm) each 439.00 Add to Cart

msdsarrowAlcian Blue 8GX, C.N. #AnAn-6 (Ingrain Blue I)

C56H68N16S4Cu   F.W. 1298.88   CAS #12040-44-7 Dye Content: Approx. 50% Solubility: Water-soluble

Used as a fixative: 0.5-2% Alcian Blue in Glutaraldehyde fixative enhances the preservation and constrast of the cell coat and intercellular substances. Used as a stain: For staining cell surface materials and cartilage components. Schofield, et al., Histochem J. 7, 139,1975 Used as a wetting agent: Sommer, J. R (1977). To cationize glass. J.Cell Bio. 75, 245a. Alcian Blue and Ruthenium Red were used to study cartilage ultrastructure. Thyberg, J., Lohmander, S., and Friberg, U., J. Ultrastruct. Res., 45, 407 (1973).

RT 10350   25g 190.00 Add to Cart

Technical Tip

Purification of Alcian Blue

msdsarrowCongo Red Solution

RT 26090-05 Alcoholic Congo Red, 0.2% Solution 500 ml 33.00 Add to Cart
RT 26090-06 Alcoholic Congo Red, 0.2% Solution 1 L 54.00 Add to Cart

msdsarrowAlizarin Red S, Certified, C.N. #DcAr-5

(Alizarin Camine, Mordant Red 3) C14H7O7SNa.H2O   F.W. 360.28   CAS #130-22-3 Solubility: 7.69% Water; 0.1% Alcohol.

Certified for Staining Bone. Stain for nervous tissue in small invertebrates, and for sections of nervous tissue. Lee, "The Microtomist's Vade-Mecum", 10th Ed., The Blakiston Co., Phila., 1937; Dawson, Stain Tech., 1, 123 (1926); Lundvall, Anat. Anz., 27, 521 (1905); 137. Use to differentiate bone from cartilage in mammalian embryos; used with Toluidine Blue. William, Stain Tech., 16, 23 (1941)

RT 10360   25g 39.00 Add to Cart

msdsarrowAluminum Oxide 90 Basic

Al2O3 F.W. 101.96 CAS 1344-28-1

Aluminum Oxide 90 Basic (63-200 Micron)  for use with Technovit® 9100.

RT 10400 Aluminum Oxide 90 Basic 500 g 80.00 Add to Cart

msdsarrow12Amphibian Ringer Solution

Ingredients: NaCl; KCl; CaCl2·6H2O and Distilled water.

RT 11762-05 Amphibian Ringer Solution 500 ml 23.00 Add to Cart
RT 11762-10 Amphibian Ringer Solution 1 L 33.00 Add to Cart

msdsarrowAmmonium Hydroxide, 28% Reagent, A.C.S.

NH4OH   F.W. 35.05   CAS #1336-21-6 Specific Gravity: 0.91 Assay 28-30%

Specifications:

Residue After Ignition   0.002%
Carbon Dioxide   0.002%
Chloride   0.5 ppm
Phosphate   2 ppm
Sulfate   2 ppm
Heavy Metals   0.5 ppm
Iron   0.2 ppm
RT 10600   450 ml 18.00 Add to Cart
RT 10601   4x450 ml 66.00 Add to Cart

msdsarrowAmmonium Molybdate, Reagent, 99%

(Molybdic acid, ammonium salt) (NH4)6MO7O24 ·4H2O   F.W. 1235.86   CAS #12027-67-7

Used in the negative staining of viruses for TEM. Modifications caused by uncoupling agents. Muscatello, U., et al, J. Ultrastruct., Res., 52, 2 (1975)

RT 10500   100g 37.00 Add to Cart

msds arrowAmyl Acetate, Reagent, A.C.S.

CH3COOC5H11   FW 130.18   CAS #628-63-7 Boiling Range: 140.0°C-150.0°C Specific Gravity @ 25°C: 0.870-0.875 A.C.S. Specifications:

Acidity (as CH3COOH)   0.01%
Non-volatile Matters   0.01%
Water (W/W)   0.2 %
RT 10800 (Plastic coated btl.) (946ml) 1qt 60.00 Add to Cart
RT 10810   4x1qt 225.00 Add to Cart

msdsarrowAmyl Acetate, Purified, Glass Distilled

CAS #628-63-7

RT 10815 (Plastic coated btl.) 100 ml 16.00 Add to Cart
RT 10816   4x100 ml 60.00 Add to Cart

msdsarrowAniline Blue, Water Soluble, C.N. #DcK-11

(Acid Blue 22; China Blue; Cotton Blue; Marine Blue V; Soluble Blue 3M, 2R; Water Blue) C32H25N3O9S3Na2   F.W. 737.74   CAS #28631-66-5

Solubility: 50% Water; 0.0% Alcohol; 4.5% Cellosolve; 7.8% Glycol; 0.0% Xylene Used in connective tissue staining procedures to stain collagen. Used in differential staining. Snodgress el al., Lab Invest. 26:329, 1972.

RT 10840   25g 32.00 Add to Cart

arrowAntibody Diluent

Buffer for diluting your primary and secondary antibodies, especially if they were stored for a while, even at -20 in glycerol, or in refrigerator. Nonspecific binding of the antibodies, negative effects of disturbing substances and low or medium affinity cross-reactivities of the antibodies will be minimized, making your result more reliable. Excellent for IHC (frozen and formalin sections), flow cytometry on fixed cells, Western Blot and other immune assays.

When used in pathology, in also greatly reduces non-specific reactivity of human serum components and immunoglobulins in tissue, vessels and cells with mouse antibodies used on section.

For especially "trouble"-giving antibodies, as well as for in situ PCR applications, this dilutent may also be used as a washing buffer, preventing secondary binding of your analystes during washing.

4-8°C 62713 Antibody Diluent msds 50 ml 150.00 Add to Cart
4-8°C 62714 Antibody Diluent 125 ml 325.00 Add to Cart
4-8°C 62715 Antibody Diluent 500 ml 1,250.00 Add to Cart
4-8°C 62713-01 Antibody Diluent for Frozen Sections 50 ml 152.00 Add to Cart
4-8°C 62714-01 Antibody Diluent for Frozen Sections 125 ml 325.00 Add to Cart
4-8°C 62715-01 Antibody Diluent for Frozen Sections 500 ml 1,250.00 Add to Cart

arrowAraldite Resin

W.P.E. number included on each bottle.

RT 10900 Araldite 502; CAS #84-74-2, Specific Gravity: 1.13 msds 450 ml 18.00 Add to Cart
RT 10910 Araldite 506; CAS #25068-38-6, Specific Gravity: 1.13 msds 450 ml 18.00 Add to Cart
RT 10920 Araldite 6005; CAS #3101-60-8, Specific Gravity: 1.16 msds 450 ml 18.00 Add to Cart
RT 10930 Araldite 507; CAS #26447-14-3, Specific Gravity: 1.14 msds 450 ml 18.00 Add to Cart

technical data sheettechnical data sheettechnical data sheetarrowAraldite Embedding Kits

Please see listing in Embedding Kits.

RT 13900 Araldite 502 Kit consists of: Kit 43.00 Add to Cart
450 ml Araldite 502 msds
450 ml DDSA; Specially Distilled msds
*50 ml DMP-30 msds
RT 13920 Araldite 6005 consists of: Kit 46.00 Add to Cart
450 ml Araldite 6005 msds
450 ml DDSA; Specially Distilled msds
100 ml BDMA msds
225 ml DBP msds
RT 13940 Araldite-EMbed (Mollenhauer’s Kit): Kit 64.00 Add to Cart
450 ml EMbed-812 msds
450 ml Araldite 502 msds
450 ml DDSA; Specially Distilled msds
*50 ml DMP-30 msds

Technical Tips

For Araldite RD-1 see BGE. For Araldite RD-2, see BDE

msdsarrowAuramine O, Certified, C.N. #DcAu-4

(Pyoctanunum aureum; Basic Yellow 2; Pyoktanin Yellow; Canary Yellow) C17H21N3HCl·H2O   F.W. 321.86   CAS # 2465-27-2 Solubility: 1.0% Water; 4.0% Alcohol; 1.2% Cellosolve; 1.75% Glycol; 0.05% Xylene

RT 10850   25 g 78.00 Add to Cart

msdsarrowAzocarmine G

(Acid red 101; Basalan Carmine GE; Rosinduline) C28H17N3Na2O6S2   F.W. 601.57   CAS #25641-18-3 Solubility: 1.0% Water; 0.1% Alcohol; 1.75% Cellosolve; 4.5% Glycol; 0.01% Xylene

RT 11150   5 g 37.00 Add to Cart

msdsarrowAzur A (Methylene Azur A)

C14H14ClN3S   F.W. 291.80   CAS #531-53-3 Solubility: Water-soluble; Alcohol-soluble

RT 11240   10g 80.00 Add to Cart

msdsarrowAzur B (Azur I; Methylene Azur B)

C15H16N3SBr   FW 350.29   CAS #531-55-5

Solubility: Water-soluble; Alcohol-slightly soluble. An important component of Giemsa's stain for blood protozoa. Used for staining semi-thin sections of plant tissue. Hoefert, L.L. (1968). Polychromatic stains for thin sections of Beta embedded in epoxy resin. Stain Tech. 43:145

RT 11200   10g 52.00 Add to Cart

msdsarrowAzur II

(Azur B/Methylene Blue 1:1)   CAS #37247-10-2

  11210   10g 38.00 Add to Cart

msdsarrowAzur II Eosin

(Azur B/Methylene Blue/Eosin 1:1:1)   CAS #53092-85-6

RT 11220   10g 56.00 Add to Cart

arrowAzur Mixture Solution

Please see Giemsa Solution.

msds arrowBasic Fuchsin, Certified, C.N. #DcFb-26

(Rasaniline Chloride; Magenta; Basic Violet 14) C20H19N3.HCl   F.W. 337.86   CAS #632-99-5

Berkowitz et al., (1968). Selective staining of nervous tissue for light microscopy following preparation for electron microscopy. J. Histochem. Cytochem. 16:808. Sato, T., and Shamoto, M (1973). A simple rapid polychrome stain for epoxy-embedded tissue. Stain Technol. 48:223.

RT 11260   25g 80.00 Add to Cart

msdsarrowBasic Green 4, Certified, C.N. #DcMg-11

(Malachite Green Oxalate; Diamond Green B; BX or P Extra; Light Green N, Solid Green O; Victoria Green B or WB) 2(C23H25N2).2(C2H2O4).C2O4   F.W. 927.03   CAS #2437-29-8 Solubility: 10.0% Water; 8.5% Alcohol; 5.5% Cellosolve; 7.0% Glycol; 0.0% Xylene. Used as a bacteria stain and a counterstain for botanical material.

RT 18100   25g 42.00 Add to Cart

msdsarrowBenzoin, U.V. Catalyst

C6H5CH(OH)COC6H5   F.W. 212.25   CAS #579-44-2 m.p. 134-136°C

RT 11270   100g 12.00 Add to Cart

msdsarrowBenzoin Ethyl Ether

(ethoxy phenylacetophenone) C6H5COCH(CO2H5)C6H5   F.W. 240.31   CAS #574-09-4 m.p. 56-59°C

RT 11280   50g 45.00 Add to Cart

msdsarrowBenzoin Methyl Ether, UV Catalyst

[alpha-Ethoxy-alpha-phenylacetophenone) C6H5CH(OCH3)COC6H5   F.W. 226.28   CAS #3524-62-7 Assay.>98% m.p. 47 - 49°C

RT 11290   10g 42.00 Add to Cart

msdsarrow(BDMA), Benzyldimethylamine

N-Benzyl-N, N-Dimethylamine C6H5CH2N(CH3)2   FW. 135.21   CAS #103-83-3 b.p. 177-180 °C An amine accelerator for polymerization of epoxy resins. A direct alternative to DMP-30 with a longer shelf life and better penetration. Purity: 98%

  11400   100 ml 15.00 Add to Cart
  11400-25   25 ml 8.00 Add to Cart

Technical Tip

Use of Accelerators for Epoxy Resins

msds arrowBiebrich Scarlet, Water Soluble

(Bonceau BS; Acid Red 66; Croceine Scarlet; Double Scarlet BSF) C22H14N4Na2O7S2   F.W. 556.4   9 CAS #4196-99-0 Solubility: 5.0% Water; 0.25% Alcohol; 1.5% Cellosolve; 1.5% Glycol; 0.0% Xylene

RT 11265   25g 28.00 Add to Cart

arrowBioBond; Tissue Section Adhesive

Please see listing in Adhesives and Mountants.

arrowBiomeda Mounting Media

Please see listing in Adhesives and Mountants.

arrowBioMount

Please see listing in Adhesives and Mountants.

msdsarrowBismarck Brown Y

(Basic Brown 1; Basic Brown G, GX, GXP; Vesuvine; Leather Brown; Manchester Brown; Phenylene Brown) C18H18N8·2HCl   F.W. 419.32   CAS #10114-58-6 Solubility: 1.5% Water; 3.0% Alcohol; 3.0% Cellosolve; 7.9% Glycol; 0.05% Xylene

RT 11310   25g 20.00 Add to Cart

msdsarrowBismuth Carbonate, Basic, Purified

(Bismuth Subcarbonate) Approx. (BiO)2CO3   CAS # 5892-10-4 Used for staining synapses. Pfenninger, K., Sandri, C., Albert, K. and Eugster, C.H. (1969). Contribution of the problem of structural organization of the presynaptic area. Brain Res., 12:10

RT 11420   25g 27.00 Add to Cart

arrowBismuth Stain Kit

Bismuth specifically stains Golgi beads and interchromatin granules after fixation with glutaraldehyde, whereas it stains nucleoli, basic protein, and biogenic amines after formaldehyde fixation. Bismuth staining of sections is relatively nonspecific: it can stain glycogen, lysosomes, ribosomes, polysaccharides, ferritin, and DNA. M.A. Hayat, "Basic Techniques for TEM" p. 184 (1986).

1. Specific En Bloc Staining (Locke & Huie, 1977)
RT 11430 Bismuth Stain Kit consists of: Kit 35.00 Add to Cart
10x5 ml Solution A (Sodium Tartrate, NaOH and Bismuth Subnitrate) msds
10x10 ml Solution B (.2M Triethanolamine-HCl buffer, pH 7.0) msds
2. Enhancing Contrast in General (Riva, 1974)

Kit contains: 5x10 ml Solution A(Sodium tartrate in NaOH) 5x200mg Ingredient B (Bismuth subnitrate)

RT 11434   Kit 22.00 Add to Cart
msds3. Staining of Mucosubstances & Polysaccharides (Ainsworth et al., 1972)

Kit contains: 10x10 ml Solution A (Periodic acid, Ethanol, Sodium acetate) 10x10 ml Solution B (Sodium tartrate, NaOH, Bismuth subnitrate)

RT 11436   Kit 40.00 Add to Cart

msdsarrowBismuth Subnitrate, Crystalline

(Bismuth Nitrate, Basic; Bismuth Oxynitrate Hydrate) 4BiNO3(OH2).BiO(OH)   F.W. 1461.99   CAS #1304-85-4 Bismuth staining for light and electron microscopy. M .Locke and P. Huie, Tissue & Cell, 9, 347 (1977); G.L. Brown & M. Locke, ibd. 10, 365 (1978)

RT 11450   25g 26.00 Add to Cart

msdsarrowBorax Tetra, Sodium Borate, Reagent, A.C.S.

(Sodium Tetraborate, Decahydrate) Na2B4O7 .10H2O   FW. 381.37   CAS #1303-96-4 A.C.S. Specifications

Assay   99.5-105%
Calcium   0.005%
Chloride   0.001%
Insoluble Matter   0.005%
Iron (Fe)   5ppm
pH of 0.01M Solution @ 25°C   9.15-9.20
Other Heavy Metals (as Pb)   0.001%
Phosphate (PO4 )   0.001%
Sulfate (S04)   0.005%
RT 21130 500g   23.50 Add to Cart

msdsarrowBoric Acid, Reagent, Powder, A.C.S.

H3BO3   FW 61.83   CAS #10043-35-3 Assay 99.95% minimum. A buffer component in Tris-EDTA-Borate buffer. Also used in Silver-Methenamine buffer. Swift, J.A. (1968). The Electron histochemistry of cystine-containing proteins in thin transverse sections of human hair. J.R. Microsc. Soc. 88,449.

RT 11460   500g 44.00 Add to Cart

arrowBouin's Fixative

Please see listing in the Fixatives section.

msdsarrowBrilliant Cresyl Blue, Certified, C.N.#DcV-5

(Brilliant Blue C; Cresyl Blue 2RN) C17H21N4OCl   F.W. 332.84   CAS #10127-36-3 Solubility: 3.0% Water; 2.6% Alcohol; 2.25% Cellosolve; 8.0% Glycol; 9.5% Xylene

RT 11465   10g 52.00 Add to Cart

msdsarrowBromophenol Blue, Reagent, A.C.S.

(3',3",5' 5"-Tetrabromophenol-sulfonthalein) C19H10O5SBr4   F.W. 669.97   CAS #115-39-9

  11470   5g 25.00 Add to Cart
  11471   25g 88.00 Add to Cart

arrowBuffers; Prepared

Please see our complete listing of Prepared Buffers.

msdsarrow1,4-Butanediol Diglycidyl Ether;

(Araldite RD-2) C10H18O4   F.W. 202.2   CAS #2425-79-8 Approx. 70% Specific Gravity: 1.10

RT 11800   100 ml 50.00 Add to Cart

msdsarrow2-Butoxy Ethanol

(Butyl Cellosolve®; Ethylene Glycol Monobutyl Ether) CH3(CH2)3OCH2CH2OH   F.W. 118.18   CAS #111-76-2 Specific Gravity: 0.90 b.p. 169°C

RT 11820   450 ml 18.00 Add to Cart

msdsarrowButvar B-98 Powder

CAS #63148-65-2 (Terpolymer of polyvinyl butyl, polyvinyl alcohol & polyvinyl acetate) Polyvinyl butyl resin for thin film support. Stronger than Formvar and more sticky, resulting in a firm attachment for slotted or meshed grids. The standard solution is 0.25% Butvar B-98 in Chloroform. Handley, D.A., et. al., Ultramicroscopy, 4,479 (1979).

RT 11850   100g 12.00 Add to Cart

msdstdsarrowButvar B-98 Solution

Stock solution: 1.0% in Chloroform.

RT 11860   100 ml 16.00 Add to Cart

Technical Tip

Tips for Butvar B-98 Support Films

msdsarrown-Butyl Acetate, Reagent, A.C.S.

CH3COO(CH2)3CH3   F.W. 116.16   CAS #123-86-4 b.p . 127°C Specific Gravity: 0.88

RT 11900   450 ml 29.50 Add to Cart

msdsarrown-Butyl Alcohol, Reagent, A.C.S.

(Butanol) CH3CH2CH2CH2OH   F.W. 74.12   CAS # 71-36-3

Specific Gravity:   0.81
Assay   99.4%
Residue after evaporation   0.005%
Titratable acid   0.0008meq/g
Butyl Ether   0.2%
Water   0.1%
RT 11920   450 ml 29.00 Add to Cart
RT 11921   4x450 ml 100.00 Add to Cart

Technical Tip

Preservation of Phospholipids

msdsarrow(BGE), n-Butyl Glycidyl Ether, (Araldite RD-1)

(Butoxymethyl oxirane) C7H14O2   F.W. 130.19   CAS #2426-08-6 b.p. 166-168°C Specific Gravity: 0.912

RT 12000   100 ml 7.00 Add to Cart

msdsarrown-Butyl Methacrylate, Monomer

CH2C(CH3)CO2CH2CH2CH2CH3   F.W. 142.20   CAS #97-88-1 b.p. 162-164°C
Stabilized with 10ppm MEHQ. (Hydroquinone, Mononethylether)

RT 12100   225 ml 15.00 Add to Cart

Certificates of Analysis


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