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Electron Microscopy Sciences

Chemicals for Microscopy

Certificates of Analysis

arrow13EA to FIRM

msdsarrowEA Counterstain, Gill's Modified Solution

For use with Papanicolaou stain; a general tissue stain. Ready-to-use.

RT 26050-05 EA Counterstain, Gill's Modified 500 ml 51.00 Add to Cart
RT 26050-06 EA Counterstain, Gill's Modified 1 L 92.00 Add to Cart

arrow12EARLE's Balanced Salt Solutions - EBSS

All of our Earle's Balanced Salt Solution contains: Potassium Chloride, Sodium Chloride, Sodium Phosphate Monobasic, and D-glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All of the solutions are prepared 1X strength.

Ingredients F.W. Formula I Formula II Formula III
KCl 74.56 X X X
NaCl 58.44 X X X
NaH2PO4·H2O 137.99 X X X
D-Glusose 180-16 1g/L X 1g/L
MgSO4·7H2O 246.48 X X X
CaCl2,·2H2O 147.02 X X X
NaHCO3 84.01 X X X
Phenol Red   ---- 0.015g/L 0.050g/L
RT 11765-05 EBSS, Formula I, 0.157M msds 500 ml 19.00 Add to Cart
RT 11765-10 EBSS, Formula I, 0.157M 1 L 24.00 Add to Cart
RT 11766-05 EBSS, Formula II, 0.152M msds 500 ml 19.00 Add to Cart
RT 11766-10 EBSS, Formula II, 0.152M 1 L 24.00 Add to Cart
RT 11767-05 EBSS, Formula III, 0.157M msds 500 ml 19.00 Add to Cart
RT 11767-10 EBSS, Formula III, 0.157M 1 L 24.00 Add to Cart

msdsarrow12EDTA, Disodium Salt, Dihydrate, A.C.S.

(Ethylenediamine Tetraacetic acid)
Na2C10H14O8·2H2O   F.W. 372.20   CAS #6381-92-6
A.C.S.

Specifications:

Assay. 99+%
Insoluble matter max 0.005%
Nitrilotriacetic acid max 0.1%
Heavy Metal (as Pb) max 0.005%
Iron max 0.01%

Organic chelating for staining glutaraldehyde fixed tissues, pre-stained with uranyl acetate, followed by lead citrate. Bernard, W., J. Ultrastruct. Res., 27. 250 (1969)

RT 15200   100g 62.00 Add to Cart

msdsarrow12EDTA Buffer pH 8.5 (10x)

Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C.DO NOT FREEZE
Intended Use:1X buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10 X, pH 8.5 antigen retriever solution.
Preparation of Working Solution:Dilute this 10X buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1X buffer can be stored at 2-8°C
4-8°C 64142-09 EDTA Buffer pH 8.5 100 ml 75.00 Add to Cart
4-8°C 64142-10 EDTA Buffer pH 8.50 1000 ml 145.00 Add to Cart

msdsarrowEhrlich's Hematoxylin

Stain selectivity for nuclei. Ready-to-use.

RT 26040-05 Ehrlich's Hematoxylin 500 ml 34.00 Add to Cart
RT 26040-06 Ehrlich's Hematoxylin 1 L 60.00 Add to Cart

msdsTechnical Data Sheetarrow12EMbed 812

CAS # N.A.
Specific Gravity: 1.08-1.10
EMbed 812 has the same characteristics as Epon 812, physically, and provides the same results in preservation, handling, curing and sectioning. EMbed 812 may cause etching on selected plastics. All formulas for mixing and using Epon 812 can be applied to EMbed 812, e.g. Luft's procedure, Mollenhauer's and EPON-ARALDITE mixtures.

RT 14900   450 ml 25.00 Add to Cart
RT 14901   4x450 ml 90.00 Add to Cart

msdsmsdsmsdsmsdstechnical data sheetarrow12EMbed 812 Embedding Kit

Please see listing in Embedding Media Kits.

RT 14120   kit 64.00 Add to Cart

Technical Data Sheetarrow12EMbed 812 / DER 736 Embedding Kit

Please see listing in Embedding Media Kits.

RT 14130   kit 65.00 Add to Cart

TechnicalTip

An Epon-812 Replacement

msdsTechnical Data Sheetarrow12Entellan®

Please see listing in Adhesives and Mountants

RT 14800   100 ml 50.00 Add to Cart
RT 14802   500 ml 195.00 Add to Cart

msdsarrow12Eosin Y, Certified, C.N. #DcE-40

(Acid Red 87, Eosin Yellowish)
C2cH6Br4Na2O5   F.W. 691.88   CAS #17372-87-1

Solubility: 44.0% Water; 2.0% Alcohol; 25.0% Cellosolve; 27.5% Glycol; 0.0% Xylene.
Used with Hematoxylin for showing cell nuclei in embryos and kidneys. Conn, "Biological Stain" 9th ed., The Williams and Wilkens Co., Baltimore 1977, pg. 7.

Component of Wright's stain for blood corpuscles. Wright, J. Med. Res., 7, 138 (1902)
For staining bone marrow to show cell morphology. Bech, "Lab. Manual of Hematological Tech.", W. B. Saunders Co., Phila., 1908, p. 233

RT 14850   10g 10.00 Add to Cart
RT 14851   25g 20.00 Add to Cart

arrow12EPO-Fix Embedding Media

Please see listing in Embedding Media Kits.

arrow12Epon-812 Resin

Please see listing in Embedding Media Kits.

msdsarrow12Epon-815 Resin;

CAS # NA
Specific Gravity: 1.13
An aromatic resin.

RT 14910   450 ml 22.00 Add to Cart

Technical Data Sheetarrow12EMS Cured Epoxy Remover

Studies show that in order to get good results in positive staining of sections, the removal of cured epoxy (embedding material) as well as the reduced-osmium (fixation materials) from the sections is necessary; especially when double fixation with an Aldehyde and Osmium tetroxide is employed to process specimens for E.M. We offer a CURED-EPOXY-REMOVER kit, which removes the polymerized resin as well as the reduced-osmium. The structure of the resin free section will remain unchanged.

RT 14942 Cured Epoxy Remover Kit consists of: kit 75.00 Add to Cart
Acetone, Reagent Grade msds
Ethyl Alcohol msds
Methyl Alcohol msds
Picric Acid msds
Benzene msds

TechnicalTip

Re-Embedding of Tissue Poorly Embedded

msdsarrow12Epoxy Tissue Stain, Ready-to-use

Toluidine Blue and Basic Fuchsin. For staining semi thin sections of epoxy embedded tissues.
Spurlock, B.O., (1966) et al., Am. J. of Clin. Path.

RT 14950   100 ml 20.00 Add to Cart

msdsarrow12Erythrosin B, Certified, C.N. #DcEr-5

(Acid Red 51, Erythrosin extra Bluish; Eosin J; Iodeosin B; Pyrosin B)
C20H6O5I4Na2   F.W. 879.87   CAS #16423-68-0
Solubility: 10.0% Water; 5.0% Alcohol; 7.0% Cellosolve; 8.25% Glycol; 0.0% Xylene.

RT 15010   10 g 23.00 Add to Cart

msdsERL 4221-An ERL-4206 Replacement

Due to the supply shortage of ERL-4206 ERL-4221 has now been specially formulated to replace it. ERL 4206 is one of the main components of the low viscosity (Dr. Spur) embedding resin kit.

ERL-4221 is a Cycloaliphatic Epoxide Resin.

Specifications:

Specific Gravity (H2O=1): 1.173 @ 20°C/20°C
Flash Point – Closed Cup: 118°C (245°F)
Boiling Point (760mm/Hg): >250°C (>482°F)
RT 15004 ERL-4221 225 ml 21.00 Add to Cart
RT 15005 ERL-4221 4x225 ml 74.00 Add to Cart

msdsarrow12Ethyl Alcohol, Specially Denatured

C2H5OH   F.W. 46.07   CAS #64-17-5
Blended 95% Ethanol and 5% Methanol
Suitable for all routine histology/cytology preparations.

Specifications:

Evaporation Residue 5ppm max.
Water 0.1%
Filtered SDA-3A 95%
RT 15050   1qt 21.00 Add to Cart
RT 15051   4x1qt 76.00 Add to Cart

msdsarrow12Ethyl Alcohol, Anhydrous

(Ethanol) - 200 Proof
CH3CH2OH   F.W. 46.07   CAS # 64-17-5

Specifications:

Specific Gravity <0.7942
Purity >99.9%
Acidity <0.002%
Water (Karl Fisher) <0.1%
Non-Volatile Matter <0.0025%
RT 15055   450 ml 20.00* Add to Cart
RT 15056   4x450 ml 70.00* Add to Cart
RT 15058   1 liter 35.00 Add to Cart

*Tax has already been paid

msdsarrow12Ethylene Dichloride, Reagent, A.C.S.1,2-Dichloroethane

Please see 1,2-Dichloroethane.

RT 13250   450 ml 25.00 Add to Cart
RT 13251   4x450 ml 78.00 Add to Cart

msdsarrow12Ethylene Glycol Dimethacrylate

(Ethylene dimethacrylate)
C10H14O4   F.W. 198.22   CAS #97-90-5

Inhibited with 100 ppm HQ. (Hydroquinone)

Specific Gravity: 1.053
b.p. 66-68°C
0-5°C 15300   225 ml 36.00 Add to Cart

msdsarrow12Ethylene Glycol Monoethyl Ether, Purified

(Cellosolve; 2-Ethoxyethanol; Ethyl Glycol)
HOCH2CH2OCH2CH3   F.W. 90.12   CAS #110-80-5

Suitable for histological use.

Specifications:

bp 133-135°C
Specific Gravity @20°C: 0.931
Assay >99.5%
Water <0.1%
Residue after evaporation <0.001%
0-5°C 15310-01   1L 39.00 Add to Cart
0-5°C 15310-06   6x1L 210.00 Add to Cart

msdstechnical data sheetEukitt™ Mounting Mediumarrow12EUKITT® Mounting Medium

EUKITT® classic has been on the market place since 1953 and has proven to be useful due to its distinct characteristics: Fast Curing, Crystal clear optically with a universal refractive index (1.49). As well, it offers good fluidity, low shrinkage and is easy to use. The EUKITT® classic may be used either manually or in an automated mode.

The product holds up well in the presence of water and trace levels of Alcohol. It is very well suited for all pale color preparations as well as colorless preparations.

EUKITT® has a neutral pH and offers extended storage times of all slide preparations for years without any sign of cracking or discoloration. The medium itself is colorless and does not change the color and or the structure of the samples mounted with the medium. EUKITT® is temperature resistant to heat and cold (-170°C), as well as humidity, daylight and UV light.

Once the EUKITT® bottle is opened it is advantageous to seal the bottle tightly as soon as you are done using it. If the medium becomes thickened you can add a small amount of xylene to dilute it.

Specifications

Refractive index n[20°C] 1.49
Viscosity 250-450 mPa*s
Curing time 20 minutes
No yellowing after long storage
No brittle fracture
No self-fluorescence under UV light
RT 15320 EUKITT® Mounting Medium 100 ml 70.00 Add to Cart
RT 15322 EUKITT® Mounting Medium 500 ml 190.00 Add to Cart

EUKITT® Classic Mounting Mediumarrow12EUKITT® Classic Mounting Medium

The gold-standard of mounting media for cover slipping machines.

EUKITT® Classic was formulated for use in Automated Coverslipping machines. Slide preparations with EUKITT® Classic exhibit minimal discoloration and perfect drying without any bubbles and streaks. With EUKITT® Classic ideal viscosity (500-750 mPa's) it is a perfect formulation for all automated machines.

Supplied in a wide neck, unbreakable aluminium bottle. The product has a shelf life of 3 years.

  • Fast drying for rapid results
  • Neutral and colorless
  • Well proven formulation
  • Long-term stability

Specifications

Refractive index n[20°C] 1.49
Viscosity 500-750 mPa*s
Drying time 20 minutes
No yellowing after long storage
No brittle fracture
No self-fluorescence under UV light
RT 15320-10 EUKITT® Classic Mounting Medium 100 ml 73.00 Add to Cart
RT 15322-10 EUKITT® Classic Mounting Medium 500 ml 215.00 Add to Cart

EUKITT® Neo Xylene Free Mounting Mediumarrow12EUKITT® Neo Xylene Free Mounting Medium

The high-quality classic of mounting media now xylene free.

EUKITT® Neo is xylene-free and characterized by fast curing, crystal-clear optics, low fluorescence, favorable refractive index, good fluidity and low shrinkage. The preparations are stable without cracking and discoloration. EUKITT® Neo is colorless and does not change shape, structure orcolor of the material to be examined. The unique formulation of this version of EUKITT® uses self sustaining raws that have no solvent base at all.

The pleasant odor facilitates working in the laboratory.

Specifications

Refractive index n[20°C] 1.48
Viscosity 250-450 mPa*s
Drying time 20 minutes
No yellowing after long storage
No brittle fracture
No self-fluorescence under UV light
RT 15320-20 EUKITT® Neo Mounting Medium 100 ml 72.00 Add to Cart
RT 15322-20 EUKITT® Neo Mounting Medium 500 ml 205.00 Add to Cart

msdsEUKITT® Neo Specialarrow12EUKITT® Neo Special Mounting Medium

Highly viscous xylene free mounting medium.

  • Faster drying for rapid results
  • Higher viscosity than EUKITT® Neo
  • Neutral and colorless
  • Long-term stability

EUKITT® Neo Special is another xylene free product. EUKITT® Neo Special is characterized by faster curing and slightly higher viscosity than EUKITT® Neo. Users of EUKITT® Classic will appreciate the xylene free replacement. The product retains crystal-clear optics, low fluorescence, favorable refractive index, good fluidity and low shrinkage properties. The preparations are stable without cracking and discoloration.

EUKITT® Neo Special is colorless and does not change shape, structure or color of the material to be examined. The use of sustainable, renewable raw material as a solvent completes the modern formulation approach.

The pleasant odor facilitates working in the laboratory. Supplied in wide neck, unbreakable aluminum bottles. The product has a shelf life of 3 years.

Specifications

Refractive index n[20°C] 1.48
Viscosity 500-750 mPa*s
Drying time 15 minutes
No yellowing after long storage
No brittle fracture
No self-fluorescence under UV light
RT 15320-25 EUKITT® Neo Special Mounting Medium 100 ml 75.00 Add to Cart
RT 15322-25 EUKITT® Neo Special Mounting Medium 500 ml 218.00 Add to Cart

msdsEUKITT® UV Mounting Medium

arrow12EUKITT® UV Mounting Medium

EUKITT® UV is the first mounting media of its kind to eliminate the need to be concerned about the vaport pressure of the solvent used.

EUKITT® UV is a UV-curing mounting medium with all the well-known beneficial properties of the gold standard series EUKITT® and a true innovation in the world of mounting. EUKITT® UV does not contain any solvents, therefore only 50% of the usual amount is needed compared to conventional systems. While using EUKITT® UV almost no shrinkage can be detected.

The mounting medium may be used on tissue sections in non-polar solvents such as xylene or xylene substitutes as well as directly on sections in pure alcohols (EtOH, iPrOH, etc.).

The viscosity of EUKITT® UV is very user-friendly. After being applied to the tissue, the cover glass can be placed as usual. Subsequently, a transfer break of at least 120 sec. is maintained prior to irradiation in order to ensure an ideal penetration into the tissue.

When using the specially tuned LED CCFL UV curing machine EUKITT® UV cures in just 30 seconds.

Specifications

Refractive index n[20°C] 1.48
Viscosity 350-550 mPa*s
Curing time 10-30 seconds
No yellowing after long storage
No brittle fracture
Suitable for all tissue types
Shelf life of a 100-ml bottle at room temperature: 6 months
RT 15320-30 EUKITT® UV Mounting Medium 100 ml 88.00 Add to Cart

EUKITT® Microfix™ Mounting Mediumarrow12EUKITT® Microfix™ Mounting Medium

MICROFIX™ follows the proven, xylene-based approach of O. Kindler ORSAtec. MICROFIX™ is characterized by fast curing, crystal-clear optics, low fluorescence, ideal refractive index, good flowability and low shrinkage. MICROFIX™ is colorless and does not change shape, structure or color of the material to be examined. These characteristics are already known from the classic EUKITT®. What is new is the streamlined and optimized synthesis and filling process, and a still further optimized selection of our raw materials.

Specifications

Refractive index n[20°C] 1.49
Viscosity 250-450 mPa*s
Drying time 20 minutes
No yellowing after long storage
No brittle fracture
No primary fluorescence under UV light
RT 15320-35 EUKITT® Microfix™ Mounting Medium 100 ml 54.00 Add to Cart
RT 15322-35 EUKITT® Microfix™ Mounting Medium 500 ml 149.00 Add to Cart

msdsarrow12Farnesol

(3,7,11-Trimethyl-2,6,10-dodecatrien-1-ol)
C15H26O   F.W. 222.57   CAS #4602-84-0
Specific Gravity: 0.886
Mixture of Stereoisomers: Approx. 90% Synthetic

0-5°C 15340   10 ml 24.00 Add to Cart

msdsarrow12Fast Green FCF, Certified, C.N. #DcGf-12

(Food Green 3)
C37H34O10N2S3Na2   F.W.808.86   CAS #2353-45-9

Solubility: 4.0% Water; 9.0% Alcohol; 8.0% Cellosolve; 6.25% Glycol; 0.0% Xylene

The main advantage of this dye is its resistance to fading. Mammalian tissue stain for visualizing collagen, muscle, cystoplasma, cells and cornified epithelium. Kornhauser, Stain Tech., 18,95 (1943); 20, 23 (1945); Lillie, Arch. Path., 29, 705 (1940). Modified Feulgen stain for visualizing chromosomes in root tips and other meristematic tissue. DeTomasi, Stain Tech., 11, 137 (1936). Used in plant histology. O. Conn, "Biological Stains" 9th ed., Williams & Wilkens Co., Baltimore, 1977, pp. 252-3; Haynes, Stain Tech., 3, 40 (1928).

RT 15500   5g 22.00 Add to Cart

arrow12Fast Green Solutions

Please see listing in Prepared Staining Solutions.

msdsarrow12Ferric Chloride, Hexahydrate, Reagent, A.C.S.

FeCl3·6H2O   F.W. 270.30   CAS #13478-10-9

Assay 97.0-102%
Insoluble Matters. 0.01%
Nitrate (about 0.001%) To Pass Test
Phosphorous Compound 0.01%
Sulfate 0.01%
Arsenic 0.002%
Copper 0.003%
Zinc 0.003%

References for use: Membrane stains

  • Gasic, G., Berwick et al (1963). Hale stain for sialic acid containing mucins. J. Cell Biol., 19:223
  • Gasic, G., Berwick, et al (1968). Positive and Negative colloidal iron as cell surface electron stains. Lab. Invest., 18:63
  • Blanquet, P.R. and Loiez, A. (1974). Colloidal iron used at pHs lower than 1 as electron stain for surface proteins. J. Histochem. Cytochem., 22:368
  • Matukafs, V.J., Panner et al (1967). Studies on ultrastructural identification and distribution of protein-polysaccharides in cartilage matrix. J. Cell Biol., 32:365
  • Benedetti, E.L. and Emmelot, P. (1967). Studies on plasma membranes IV. The Ultrastructural localization and content of sialic acid in plasma membranes isolated from rat liver and hepatoma. J. Cell Sci., 2:499
  • Rowley, J.R. (1971). Resolution of channels in the exine by translocation of colloidal iron. Pro. 29th Ann. EMSA Meet., p.352. Claitor's Pub. Division, Baton Rouge, LA
  • Nicolson, G.L. (1973). Anionic sites of human erythrocyte membranes.I. Effects of trypsin, phospholipase C, and pH on the topography of bound positively charged colloidal particles. J. Cell Biol., 57:373
  • Hendy, R. (1971). Electron microscopy of lipofucsin pigment stained by the Schmorl and Fontana technique. Histochemie, 26:311.
RT 15510 Ferric Chloride, Hexahydrate, Reagent 500g 45.00 Add to Cart

arrow12Ferric Chloride Solutions

Please see listing in Reagents for Histology.

msdsTechnical Data Sheetarrow12Ferritin Cationized:

CAS #9007-73-2

This polycationic derivative of ferritin is useful in labeling negative charges on cell surfaces and to study membrane structure. Cationized ferritin is prepared by coupling ferritin from horse spleen with N,N-dimethyl-1,3-propanediamine (DMPA). Sterile filtered solution in 0.15M NaCl.
Ferritin Concentration: 10mg per ml, coupled with DMPA.
A cationic derivative used to label negatively charged cell membranes for visualization by EM.Danon, D et al. (1972), J. Ultrastruct. Res., 38, 500

0-5°C 15550 Cationized 1ml 138.00 Add to Cart

Technical Data Sheet PDF DownloadImage Galleryarrow12Fluorophore-Infiltrated Resin Microscopy (FIRM)

Rapid staining technique that provides high-contrast fluorescence microscopy imaging of resin embedded samples, reminiscint of low magnification transmission electron microscopy.

  • Works with Epoxy and Acrylic type resins
  • Simply mount your sections on glass slides, air dry, and overlay with 100 microliters FIRM for 30 seconds, wash and coverslip with water or an aqueous mountant. View in Rhodamine channel of a standard wide field Fluorescence Microscope.
  • FIRM staining is unique in that the brightly fluorescent dye infiltrates the resin, revealing tissue structures in negative relief, with extremely high contrast and resolution.
  • Perfect for previewing specimens prior to EM Analysis
  • High Throughput, high contrast image phenotyping of model organisms such as drosophila
  • Ideal for material and food science analytical microscopy
RT 12780-05 FIRM for Acrylic Resin Sections msds 10ml 90.00 Add to Cart
RT 12780-06 FIRM for Epoxy Resin Sections msds 10ml 90.00 Add to Cart

Certificates of Analysis


Fixatives to Fluorescein arrow13arrow13