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Electron Microscopy Sciences

Chemicals for Microscopy

Certificates of Analysis

arrow13Fixatives, Prepared to Fluorescein

arrow12Fixatives, Prepared

Premixed, prebuffered fixatives, that are pH adjusted for immediate laboratory use. They are freshly prepared upon receipt of order to ensure maximum shelf life. The fixatives are filled in 20 ml glass vials (10 ml/vial). This allows for the immediate fixation of your specimens without costly and time consuming transfer associated with ampoule contained fixatives.

*If you desire any prepared fixative that is not already listed in our catalog or you have special size and packaging requirements, please call us.

0-5°C 15953 0.2% Formaldehyde/0.1% Chromic Acid in 0.1M Sodium Cacodylate Buffer, pH 7.4 10x10 ml 36.00 Add to Cart
0-5°C 15956 2% Formaldehyde/0.02% Picric Acid in 0.1M Sodium Phosphate Buffer, pH 7.2 10x10 ml 36.00 Add to Cart
0-5°C 15949 Formaldehyde/Glutaraldehyde, 2.5% each in 0.1M Sodium Cacodylate Buffer, pH 7.4 10x10 ml 37.00 Add to Cart
0-5°C 15950 Formaldehyde/Glutaraldehyde, 3% each in 0.1M Sodium Cacodylate Buffer, pH 7.4 10x10 ml 37.00 Add to Cart
0-5°C 15960 2.5% Glutaraldehyde in 0.1M Sodium Cacodylate Buffer, pH 7.4 10x10 ml 36.00 Add to Cart
0-5°C 15960-01 2% Paraformaldehyde / 2.5% Glutaraldehyde in 0.1M Sodium Cacodylate Buffer, pH 7.4 10x10 ml 27.00 Add to Cart
0-5°C 15970 2.5% Glutaraldehyde in 0.1M Millonig's Sodium Phosphate Buffer pH 7.2 10x10 ml 36.00 Add to Cart
0-5°C 15980 2.5% Glutaraldehyde in 0.1M Sorensen's Sodium-Potassium Phosphate Buffer, pH 7.2 10x10 ml 36.00 Add to Cart

msds

0.2% Formaldehyde/0.1% Chromic Acid 15953
2% Formaldehyde/0.02% Picric Acid 15956
Formaldehyde/Glutaraldehyde, 2.5% 15949
Formaldehyde/Glutaraldehyde, 3% 15950
2.5% Glutaraldehyde in 0.1M Sodium 15960
2.5% Glutaraldehyde in 0.1M Millonig's Sodium 15970
2.5% Glutaraldehyde in 0.1M Sorensen's Sodium 15980

msdsarrow12Bouin's Fixative

A fixative that is used in light microscopy for the preparation of delicate structure.

RT 15990   10x10 ml 36.00 Add to Cart

msdsarrow12B-5 Fixative

For lymph nodes.

RT 64122-05 B-5 Fixative 500 ml 64.00 Add to Cart
RT 64122-06 B-5 Fixative 1 lt 120.00 Add to Cart
RT 64122-10 B-5 Fixative 4 lt 450.00 Add to Cart

msdsarrow12Bouin's Solution

Bouin's solution can be used as a fixation and a staining fluid. Bouin's fixative is excellent for use on biopsy specimens of the gastrointestinal tract. Tissue from the endocrine system are well fixed and many antibodies react well with tissue fixed in Bouin's. Bouin's solution may be used as a routine fixative or a staining solution, but should not be used as a preservative for tissue. Tissue cannot be held in Bouin's solution for extended periods. The maximum fixation time in this solution should be less than 48 hours and should be transferred to alcohol. Packaged in leak-proof histo-containers, prefilled and ready-to-use.

Formulated with Picric Acid saturated aqueous solution 75%, Formalin (40% aqueous formaldehyde) 25% and Glacial Acetic acid 5% in de-ionized water.

RT 15990-01 Bouin's Fluid 1 L 45.00 Add to Cart
RT 15990-04 Bouin's Fluid 4 L 160.00 Add to Cart
RT 15990-10 Bouin's Fluid Pre-filled cups 50x10 ml/cs 80.00 Add to Cart
RT 15990-20 Bouin's Fluid Pre-filled cups 50x20 ml/cs 100.00 Add to Cart
RT 15990-30 Bouin's Fluid Pre-filled cups 50x30 ml/cs 45.00 Add to Cart

msdsarrow12Brasil Fixative

Ethanol, Picric Acid, Formalin, Acetic Acid, Glacial.
This fixative is most popular when glycogen is the target. The solution is similar to Bouins Solution (alcoholic) offering quick hardening and good staining with acid dyes.

RT 15995-10 Brasil Fixative 10x10 ml 40.00 Add to Cart
RT 15995-20 Brasil Fixative 100 ml 40.00 Add to Cart

arrow12Carnoy's Fluid

Stock solution.

RT 64130-05 Carnoy's Fluid msds 500 ml 26.00 Add to Cart
RT 64130-50 Carnoy's Fluid Without Chloroform msds 500 ml 24.00 Add to Cart

msdsarrow12Carson's Phosphate Buffered Formalin

Millonig's phosphate buffered formalin as modified by Carson, Martin and Lynn, by using 4% formalin in 0.1M sodium phosphate monobasic with sodium hydroxide to pH 7.2.

RT 15732-05 Carson's Phosphate Buffered Formalin 500 ml 18.00 Add to Cart
RT 15732-10S Carson's Phosphate Buffered Formalin 1 L 31.00 Add to Cart

arrow12Carson's Phosphate Buffered Paraformaldehyde

For some tissues, the use of formalin (contains 10 – 15% Methanol) is not suitable, we offer the alternative formaldehyde (prepared from paraformaldehyde – contains no methanol) for your convenience.

RT 15733-05 Carson's Phosphate Buffered Paraformaldehyde 500 ml 21.00 Add to Cart
RT 15733-10 Carson's Phosphate Buffered Paraformaldehyde 1 L 35.00 Add to Cart

msdsarrow12CytoPrep Fixative

CytoPrep Fixative forms a clear protective coating over specimens while fixing cells. It is ideal for cytological smears and it dries within 4 to 6 minutes. Good for safe storage and transportation of slides. It is water soluble and contains no chlorofluorocarbons. Comes in a 4 oz. spray can.

RT 64145-01 CytoPrep Fixative 4 oz 18.00 Add to Cart
RT 64145-12 CytoPrep Fixative 12x4 oz 198.00 Add to Cart

msdsarrow12Davidson's Fixative (Hartmann's/ Davidson's Fixative)

This is rapid fixative. Results with good nuclear detail and minimal formalin pigment. Davidson's (Hartmann's) Fixative is useful for preparing tumors, bone marrow, gynecologic specimen, fatty breast, medical biopsies. Fixation of small specimen is rapid. Exposure to the fixative should be limited to 24 hours (tissue may then be transferred to neutral buffered formalin or 70% alcohol for storage).

Based on the volume of 1125 ml solution, there are 2 parts Formalin (35-39%), 3 parts Ethanol, 1 part Glacial Acetic acid, and 3 parts tap water.

RT 64133-10 Davidson's (Hartmann's) Fixative 1 L 82.00 Add to Cart
RT 64133-14 Davidson's (Hartmann's) Fixative 4 L 232.00 Add to Cart

msdsarrow12Modified Davidson's Fixative

(14% Ethyl Alcohol, Denatured, 37.5% Formalin 37-39%, 6.25% Glacial Acetic Acid, and D.I. Water).

RT 64133-50 Modified Davidson's Fixative 1L 39.00 Add to Cart

msdsarrow12Dietrich's Fixative

Ethanol, formalin, glacial acetic acid, distilled water.

RT 15958-10 Dietrich's Fixative 10x10 ml 43.00 Add to Cart
RT 15958-20 Dietrich's Fixative 100 ml 42.00 Add to Cart

msdsarrow12Fast Frozen Fix™

Prepared fixative – This is one step quick fix for frozen sectioned tissue. Fixes tissue to slide to allow for staining procedures. Apply to most tissue types.

RT 15280-01 Fast Frozen Fix™ 100 ml 88.00 Add to Cart
RT 15280-05 Fast Frozen Fix™ 500 ml 154.00 Add to Cart

msdsAlcoholic Stain Solutionsarrow12Fixative/Decalcifier

  • A solution that fixes and decalcifies in one step.
  • Prevents loss of cellular structure.
  • Enhances hematoxylin staining.
RT 64148-01 Fixative/Decalcifier 1 L 50.00 Add to Cart
RT 64148-06 Fixative/Decalcifier 6x1 L 270.00 Add to Cart

msdsarrow12Formalde-Fresh Low Odor Solution; Histology Grade

A specially formulated odor-mask which neutralizes the formalin odor without affecting subsequent tissue processing, handling, or staining. Low odor, ready-to-use 10% formalin solution. May be used as a fixative or preservative of specimens. Its shelf life is 24 months.

RT 15678-01 Formalde-Fresh 4 L 92.00 Add to Cart
RT 15678-04 Formalde-Fresh 4x4 L 358.00 Add to Cart

msdsarrow12Buffered Formalde-Fresh, Low-Odor 10% Formalin Solution

Low odor, prepared w/v, pH 6.9 to 7.1 at 25°C. 10% formalin in Phosphate buffer according to the A.F.I.P. Manual of Histology. This solution allows for long-term storage of tissue specimens.

RT 15741-01 Low-Odor Formalin 10% Buffered 4 L 64.00 Add to Cart
RT 15741-04 Low-Odor Formalin 10% Buffered 4x4 L 229.00 Add to Cart

msdsarrow12Formalin, Neutral, Buffered 10% w/v in Acetate Buffer

Buffered with Sodium Acetate in accordance with the A.F.I.P. manual of Histology. Filtered and ready-to-use.
pH 6.0 to ph 7.1 at 20°C.

RT 15738-01 Formalin 10% Buffered in Acetate 1L 22.00 Add to Cart
RT 15738-04 Formalin 10% Buffered in Acetate 4L 54.00 Add to Cart

msdsarrow12Formalin, Neutral, Buffered 10% w/v in Phosphate Buffer

Buffered with phosphate in accordance with the A.f.I.P. manual of Histology.Filtered and ready-to-use.
pH 6.9 to 7.1 at 25°C.

RT 15740 Formalin 10% Buffered in Phosphate 450 ml 15.00 Add to Cart
RT 15740-01 Formalin 10% Buffered in Phosphate 1L 22.00 Add to Cart
RT 15740-04 Formalin 10% Buffered in Phosphate 4L 60.00 Add to cart

msdsarrow12TissuePrep Buffered 10% Formalin

Prefilled with 10% formalin Cat# 15740. Ready-to-use in pre-filled cups. Labeled in accordance to OSHA requirements, which gives you immediate information regarding handling and first aid procedures. Pre-measured in wide-mouth, leak-proof, opaque polypropylene containers which limits your exposure to formaldehyde and saves you time.

  Catalog # Cup Size Filled Pack/cs Price  
RT 15742-10 15 ml 10 ml 50 77.00 Add to Cart
RT 15742-20 30 ml 20 ml 50 98.00 Add to Cart
RT 15742-30 60 ml 30 ml 20 44.00 Add to Cart
RT 15742-60 120 ml 60 ml 20 48.00 Add to Cart

msdsarrow12Formalin Prefilled HistoTainer™

The HistoTainer™ (Empty Vials, see page 482) is half filled with 10% Neutral Buffered Formalin as a fixative. These ready-to-use prefilled containers are timer saving and make your work more efficient.

  EMS # Description Vial Size Prefilled Qty    
RT 15743-20 Formalin Prefilled HistoTainer 20 ml 10 ml 24 26.00 Add to Cart
RT 15743-40 Formalin Prefilled HistoTainer 40 ml 20 ml 24 30.00 Add to Cart
RT 15743-60 Formalin Prefilled HistoTainer 60 ml 30 ml 24 36.00 Add to Cart
RT 15743-90 Formalin Prefilled HistoTainer 90 ml 45 ml 24 42.00 Add to Cart
RT 15743-120 Formalin Prefilled HistoTainer 120 ml 60 ml 24 43.00 Add to Cart

msdsarrow12Formalin Non Buffered

Formalin 1% Non Buffered
RT 15734-01 Formalin 1% Non-Buffered 1L 20.00 Add to Cart
RT 15734-04 Formalin 1% Non-Buffered 4L 55.00 Add to Cart
msdsFormalin 2%, Non-Buffered
RT 15735-01 Formalin 2% Non-Buffered 1L 21.00 Add to Cart
RT 15735-04 Formalin 2% Non-Buffered 4L 54.00 Add to Cart
msdsFormalin 3%, Non-Buffered
RT 15735-10 Formalin 3% Non-Buffered 1L 20.00 Add to Cart
RT 15735-20 Formalin 3% Non-Buffered 4L 56.00 Add to Cart
msdsFormalin 4%, Non-Buffered
RT 15735-30 Formalin 4% Non-Buffered 1L 21.00 Add to Cart
RT 15735-40 Formalin 4% Non-Buffered 4L 55.00 Add to Cart
msdsFormalin 5%, Non-Buffered
RT 15735-50 Formalin 5% Non-Buffered 1L 22.00 Add to Cart
RT 15735-60 Formalin 5% Non-Buffered 4L 56.00 Add to Cart

arrow12Hollande's Fixative

Please see Hollande's Fixative

msdsarrow12Karnovsky's Fixative:

Please see Paraformaldehyde-Glutaraldehyde Solution Karnovsky's Fixative.

RT 15720   kit 25.00 Add to Cart
RT 15730   box of 5 kits 108.00 Add to Cart
msdsFixative Reagent Grade (Karnovsky's)

This fixative contains 3% glutaraldehyde and 2% formaldehyde in 0.1M phosphate buffer, pH 7.4.

Karnovsky's with formalin 2% and Biological glutaraldehyde 3% in 0.1m Phosphate Buffer.

RT 15731-10 Karnovsky Fixative Reagent Grade 1 L 256.00 Add to Cart
msdsKarnovsky's with paraformaldehyde solution and EM grade glutaraldehyde

Please Note: This kit can be formulated to fit your requirements. Please contact us.

RT 15732-10 Karnovsky Fixative EM Grade 1 L 282.00 Add to Cart

arrow12Paraformaldehyde, Prepared Fixatives

In addition to our complete line of Formaldehyde solutions we have now added to the line.

msdsParaformaldehyde 0.5% in 0.1M phosphate buffer pH7.4
RT 15951-10 Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer 10x10 ml 37.00 Add to Cart
RT 15951-15 Paraformaldehyde, 0.5% each in 0.1M Phosphate Buffer 500 ml 103.00 Add to Cart
msdsParaformaldehyde 1% Aqueous
RT 15953-10 Paraformaldehyde, 1% each Aqueous 10x10 ml 25.00 Add to Cart
RT 15953-15 Paraformaldehyde, 1% each Aqueous 500 ml 95.00 Add to Cart
msdsParaformaldehyde 1% in 0.1M Phosphate Buffer pH, 7.4
RT 15952-10 Paraformaldehyde, 1% each in 0.1M Phosphate Buffer 10x10 ml 39.00 Add to Cart
RT 15952-15 Paraformaldehyde, 1% each in 0.1M Phosphate Buffer 500 ml 103.00 Add to Cart
msdsParaformaldehyde 1% Glut 2% in 0.1M Phosphate buffer, pH 7.4
RT 15949-30 Paraformaldehyde 1% Glutaraldehyde 2% in 0.1M Phosphate Buffer 10x10 ml 39.00 Add to Cart
RT 15949-35 Paraformaldehyde 1% Glutaraldehyde 2% in 0.1M Phosphate Buffer 500 ml 106.00 Add to Cart
msdsParaformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate, pH 7.4
RT 15949-50 Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer 10x10 ml 42.00 Add to Cart
RT 15949-60 Paraformaldehyde 3% Glutaraldehyde 0.35% in 0.1M Sodium Cacodylate Buffer  500 ml 113.00 Add to Cart
msdsParaformaldehyde 4% and Glutaraldehyde 1% in Phosphate Buffer, pH 7.0
RT 15949-70 Paraformaldehyde 4% Glutaraldehyde 1% in 0.1M Phosphate Buffer 10x10 ml 46.00 Add to Cart
RT 15949-80 Paraformaldehyde 4% Glutaraldehyde 1% in 0.1M Phosphate Buffer 500 ml 113.00 Add to Cart
msdsParaformaldehyde 4% in 0.1M Sodium Cacodylate, pH7.4
RT 15952-10S Paraformaldehyde 4% in 0.1M Sodium Cacodylate Buffer 10x10 ml 49.00 Add to Cart
RT 15952-15S Paraformaldehyde 4% in 0.1M Sodium Cacodylate Buffer 500 ml 129.00 Add to Cart
msdsParaformaldehyde 4% in 0.1M Phosphate Buffer, pH 7.4
RT 15735-10S Paraformaldehyde 4% in 0.1M Phosphate Buffer 10x10 ml 36.00 Add to Cart
RT 15735-20S Paraformaldehyde 4% in 0.1M Phosphate Buffer 500 ml 106.00 Add to Cart
msdsParaformaldehyde 4% in 0.2M Phosphate Buffer, pH 7.4
RT 15735-30S Paraformaldehyde 4% in 0.2M Phosphate Buffer 10x10 ml 36.00 Add to Cart
RT 15735-40S Paraformaldehyde 4% in 0.2M Phosphate Buffer 500 ml 106.00 Add to Cart
msdsParaformaldehyde 4% in 0.1M Phosphate Buffer, pH 7.2
RT 15735-50S Paraformaldehyde 4% in 0.1M Phosphate Buffer 10x10 ml 42.00 Add to Cart
RT 15735-60S Paraformaldehyde 4% in 0.1M Phosphate Buffer 500 ml 110.00 Add to Cart
msdsParaformaldehyde 4% in 0.2M Phosphate Buffer, pH 7.2
RT 15735-70 Paraformaldehyde 4% in 0.2M Phosphate Buffer 10x10 ml 42.00 Add to Cart
RT 15735-80 Paraformaldehyde 4% in 0.2M Phosphate Buffer 500 ml 108.00 Add to Cart
msdsParaformaldehyde 4% in 0.1M Phosphate BufferSaline, pH7.4
RT 15735-85 Paraformaldehyde 4% in 0.1M Phosphate Buffer Saline 10x10 ml 43.00 Add to Cart
RT 15735-90 Paraformaldehyde 4% in 0.1M Phosphate Buffer Saline 500 ml 112.00 Add to Cart
msdsParaformaldehyde 4% in 0.2M Phosphate Buffer Saline, pH 7.4
RT 15735-95 Paraformaldehyde 4% in 0.2M Phosphate Buffer 10x10 ml 44.00 Add to Cart
RT 15735-100 Paraformaldehyde 4% in 0.2M Phosphate Buffer 500 ml 112.00 Add to Cart

msdsarrow12Trump's Fixative

A combination of Sodium Cacodylate, Formalin, and Glutaraldehyde. Ready to use.

0-5°C 11750   500 ml 43.00 Add to Cart
0-5°C 11752   4x500 ml 158.00 Add to Cart

Certificates of Analysis


Formaldehyde to Gill's arrow13arrow13