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Electron Microscopy Sciences

Chemicals for Microscopy

Certificates of Analysis

arrow13Hydrochloric to Mucicarmine

msdsarrow12Hydrochloric Acid Solution 0.1N

HCl   CAS #7647-01-0
Used to adjust the pH of buffers and fixative solutions.

RT 16760   100 ml 4.00 Add to Cart

msdsarrow12Hydrochloric Acid Solution 1.0N

HCl   CAS #7647-01-0
Used to adjust the pH of buffers and fixative solutions.

RT 16770   100 ml 6.00 Add to Cart

arrow12Hydrochloric Acid Solutions

For all our Hydrochloric Acid Solutions, please see listing in Reagents for Histology.

msdsarrow12HydrogenPeroxide 30% Solution-Stabilized, Reagent, A.C.S.

H2O2   F.W. 34.01   CAS #7722-84-1

Assay   29-32%
Maximum of Impurities:    
Chloride(Cl)   0.0003%
Nitrate   0.0002%
Phosphate   0.0002%
Sulfate   0.0005%
Heavy Metal   0.0001%
Iron   0.00005%
Titratable Acid   0.0006meq/g
0-5°C 16790   100 ml 70.00 Add to Cart
0-5°C 16792   450 ml 137.00 Add to Cart

msdsarrow122-Hydroxyethyl Methacrylate

Please see (GMA) Glycol methacrylate.

RT 16800   450 ml 37.00 Add to Cart

arrow12Hydroquinone Solutions

For all our Hydroquinone Solutions, please see listing in Reagents for Histology.

msdsarrow12(HPMA), 2-Hydroxypropyl Methacrylate

(Propylene glycol monomethacrylate)
C7H12O3   F.W. 144.17   CAS #27813-02-1
b.p. 70°C/1mmHg
Specific Gravity @25°C 1.028
Solubility in Water 13%
Inhibited with approx. 300ppm MEHQ
A water miscible monomer

0-5°C 16900   450 ml 30.00 Add to Cart
0-5°C 16901   4x450 ml 108.00 Add to Cart

msdsarrow12Imidazole, Reagent

(Glyoxaline)
C3H4N2   F.W. 68.08   CAS #288-32-4
Assay min 99.5%
Imidazole is a lipid-enhancing buffer agent. When used with 4% osmium tetroxide solution in the post-fixation of tissue blocks, it produces a prominent electron-opaque staining of lipid droplets with no evidence of diffusion.
Reference: Angermuller, S. Fahimi, H., Histochem. Journal 14, 823-35 (1982)

RT 16905   100g 26.00 Add to Cart

Cargille PCB-Free Immersion oilsarrow12Immersion Oils For Normal Light Microscopy:

Which Type - Which Application?

Cargille PCB-Free Immersion oils were introduced in 1972. Today, no other immersion oil meets FDA criteria. Now four standard types are available with the introduction of Type NVH, a very high viscosity oil. All Cargille Immersion Oils meet DIN 58 884 (Deutsche Institut Fur Normung e.V.)

arrow12For Normal Light Microscopy:

Types A & B, with viscosities of 150 and 1250cSt respectively, meet these requirements. The greater the gap between cover glass and objective, or condenser and slide, the more desirable high viscosity becomes. For extremely large gaps, type NVH should be used. All Cargille Immersion Oils can be blended for intermediate viscosities.

arrow1237°C Immersion Oils

A number of research projects require microscopic studies at internal body temperature. Since the index/temperature relationship is inverse, increasing temperature significantly reduces the index of the immersion oil and consequently the imagery deteriorates. This problem has been resolved by the formation of Cargille 37°C Immersion Oils, which have an index of 1.515 at 37°C.

Consideration of these oils is also suggested where stage temperatures are elevated by sub-stage illuminators and high wattage projection equipment.

arrow12Immersion Oil Type 300

Automated Hematology Systems: Use Type 300; Automated Hematology Systems depend on accurate, precisely controlled physical and optical properties of immersion oil for successful imaging and mechanical processing. Type 300 is designed and manufactured to meet the stringent requirements of this equipment, which include specialized viscosity and exacting controls for its consistency.

Immersion Oils Specifications:

OPTICAL & PHYSICAL PROPERTIES
Immersion Oil Type A B 300
Refractive Index : @23°C @23°C @23°C
  F Line (4861 A) 1.5239 1.5236 1.5238
  E Line (5461 A) 1.5180 1.5180 1.5180
  D Line (5893 A) 1.5150 1.5150 1.5150
  C Line (6563 A) 1.5114 1.5115 1.5116
Dispersion:      
  nF-nC 0.0125 0.0119 0.0123
  Abbe VD 41.3 43.2 41.8
  Abbe Ve 41.0 42.8 41.5
Temperature Coefficient (15-35°C) -0.00033 -0.00031 -0.00033
Stability (change in nD25°C after 24 hrs @ temp.)      
  at 60°C: 0 0 0
  at 100°C: 0 0 0
Fluorescence (1): Short UV: low low low
Fluorescence (1): Long UV: low low low
Color: (Gardner) 1 1 1
Viscosity:  cSt +/- 10% @ 23°C (centistokes) 150
low
1250
high
300
medium
Density: @ 23°C g/cc: .923 .923 .923
Density: (US) lb/gal 7.70 7.70 7.70
Cloud Point: <-13°C <-13°C <-13°C
Flash Point: 325°F 325°F 325°F
Neutralization No.: (mg KOH/g) 0.01 max 0.01 max 0.01 max

(1) Relative to Cedarwood Oil

RT 16907-01 TYPE A msds 1 oz 8.75 Add to Cart
RT 16907 TYPE A 4 oz 19.00 Add to Cart
RT 16907-16 TYPE A 16 oz 41.25 Add to Cart
RT 16908-01 TYPE B msds 1 oz 8.75 Add to Cart
RT 16908 TYPE B 4 oz 19.00 Add to Cart
RT 16908-16 TYPE B 16 oz 41.25 Add to Cart
RT 16917 TYPE 300 msds 1 oz 9.75 Add to Cart
RT 16917-01 TYPE 300 4 oz 21.00 Add to Cart
RT 16917-16 TYPE 300 16 oz 49.00 Add to Cart

Special Immersion Oils

For Horizontal, Inverted and Inclined Instruments and Projection Equipment:

Higher viscosities are generally required.  Type NVH meets the requirement, having a viscosity of 21,000cST.

OPTICAL & PHYSICAL PROPERTIES

Immersion Oil Type 37** NVH OVH
Refractive Index : @37°C @23°C @23°C
  F Line (4861 A) 1.5238 1.5230 1.5230
  E Line (5461 A) 1.5180 1.5178 1.5178
  D Line (5893 A) 1.5150 1.5150 1.5150
  C Line (6563 A) 1.5115 1.5118 1.5118
Dispersion:      
  nF-nC 0.0123 0.0113 0.0111
  Abbe VD 42.0 45.7 46.3
  Abbe Ve 41.7 45.4 46.0
Temperature Coefficient (15-35°C) -0.00035 -0.00034 -0.00034
Stability (change in nD25°C after 24 hrs @ temp.)      
  at 60°C: 0 0 0
  at 100°C: 0 0 0
Fluorescence (1): Short UV: low low low
Fluorescence (1): Long UV: low low low
Color: (Gardner) 1 1 <3
Viscosity:  cSt +/- 10% @ 23°C (centistokes) 1250
high
21,000
very high
46,000
very high
Density: @ 23°C g/cc: 0.917 0.919 0.918
Density: (US) lb/gal 7.73 7.67 7.66
Cloud Point: <-13°C <-13°C <-13°C
Flash Point: 325°F 325°F 325°F
Neutralization No.: (mg KOH/gm) 0.01 max 0.01 max 0.04 max

(1) Relative to Cedarwood Oil
**All values standardized at 37°C

RT 16914-01 TYPE 37 msds 1 oz 12.50 Add to Cart
RT 16909-04 TYPE 37 4 oz 25.25 Add to Cart
RT 16909-01 TYPE NVH   1 oz 11.50 Add to Cart
RT 16909 TYPE NVH 4 oz 25.25 Add to Cart
RT 16918 TYPE OVH   1 oz 12.00 Add to Cart
RT 16918-01 TYPE OVH 4 oz 25.25 Add to Cart
RT 16918-16 TYPE OVH 16 oz 68.75 Add to Cart

Immersion Oils For Fluorescence Microscopy

After years of development and field testing, we now have available two new PCB-Free Immersion Oils for Fluorescence Microscopy.

* Immersion Oil Type HF

Type DF has been discontinued by Cargille. As an alternative we are offering either a new Immersion Oil called HF which would provide low fluorescence and the correct optical properties or you can use our FF which has virtually no fluorescence but slightly less than ideal optical properties.

* Immersion Oil Type FF

Type FF has virtually no background fluorescence which makes it ideal for observing low levels of fluorescence. It is highly stable, water white, and non-hydroscopic. Type FF can be substituted for all other fluorescence microscopy immersion oils in nearly all fluorescence applications.

* Immersion Oil Type LDF

Extremely low fluorescence is achieved by Type LDF. It is the replacement of choice for the DF which is no longer being manufactured. The LDF possesses the highest resolution of the oils for fluorescence applications.

OPTICAL & PHYSICAL PROPERTIES

Immersion Oil Type LDF Type FF Type HF
Refractive Index @ 23°C:
  F Line (4861 A) 1.5239 1.4850 1.5234
  E Line (5461 A) 1.5181 1.4811 1.5180
  D Line (5893 A) 1.5150 1.4790 1.5150
  C Line (6563 A) 1.5115 1.4766 1.5117
Dispersion:  
  nF-nC 0.0125 0.0084 0.0118
  Abbe VD 41.3 57.0 43.8
  Abbe Ve 41.0 56.5 43.6
Temperature Coefficient
(15-35°C)
-0.00036 -0.00037 -0.00038
Stability (change in nD25°C after 24 hrs. @ temp.)  
  at 60°C: -0.0004 0 +0.0001
  at 100°C: -0.0043 0 +0.0006
Fluorescence (1):      
  Short UV: very low none very low
  Long UV: very low none very low
Color: (Gardner) <1 <1 <1
Viscosity: +/-10% @ 23°C (centistokes) 500
medium
170
low
700
medium
Density:      
  @ 23°C g/cc: 0.984 0.877 .9306
  (US) lb/gal 8.21 7.32 7.77
Cloud Point: <-14°C <-6°C <2°C
Flash Point: 171°F 420°F >340°F
Neutralization No.: (mg KOH/gm) <0.01 max 0.01max 0.03 max

*Relative to Cedarwood Oil

RT 16919 Immersion Oil Type LDF msds 1 oz 12.50 Add to Cart
RT 16919-01 Immersion Oil Type LDF 4 oz 28.75 Add to Cart
RT 16919-16 Immersion Oil Type LDF 16 oz 63.50 Add to Cart
RT 16915-01 Immersion Oil Type HF msds 1 oz.* 13.25 Add to Cart
RT 16915-04 Immersion Oil Type HF 4 oz 29.00 Add to Cart
RT 16916-01 Immersion Oil Type FF msds 1 oz.* 13.00 Add to Cart
RT 16916-04 Immersion Oil Type FF 4 oz 26.00 Add to Cart

* 1 oz. Applicator bottles have a glass ball rod in the cap.

arrow12Immuno-Bed™ Embedding Kit

Please see listingin Embedding Media Kits.

arrow12ImmunoHistoZyme™, Ready-to-Use

Description: ImmunoHistoZyme is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. ImmunoHistoZyme digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use:Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: Ready-to-use ImmunoHistoZyme Reagent.
4-8°C 64142-13 ImmunoHistoZyme 15 ml 75.00 Add to Cart
4-8°C 64142-14 ImmunoHistoZyme 100 ml 145.00 Add to Cart

msdsarrow12Indigo Carmine, Certified, C.N. #DcI-7

(Acid Blue 74; Carmine Blue; Indigotine 1, 1A)
C16H8N2O8S2Na2   F.W.466.36   CAS #860-22-0
Dye content: approx. 85%
Solubility: 1.3% Water; 0.0% Alcohol; 4.0% Cellosolve; 2.85% Glycol; 0.0% Xylene
Shunway's stain for animal embryos. Used with Acid Fuchsin for staining Negri bodies. Stain Tech., 5, 34 (1928)
Counterstain to Hematoxylin for staining vaginal smears. Am. J. Anat., 61, 505 (1937).

RT 16910   25g 17.00 Add to Cart

Technical Tip:

Achieving Maximum Contrast with Stain.

msdsarrow12Indium Trichloride, Anhydrous

InCl3   F.W. 221.18   CAS #10025-82-8
Chloride Content: 98%
A metal stain for nucleic acid
J. Biophys Biochem Cytol, 11,257 (1961)

RT 16920   5g 60.00 Add to Cart

msdsarrow12Ingrain Blue, C.N. #AnAn-6

Please see Alcian Blue 8GX

RT 10350   25g 190.00 Add to Cart

msdsarrow12Isopropyl Alcohol, Reagent, A.C.S.

(2-Propanol)
CH3CH(OH)CH3   F.W. 60.10   CAS #67-63-0
A.C.S. Specifications:

Color (APHA) . 10
Density @ 25°C . 0.781-0.783
Boiling Range (including 82.3°C) . 1°C
Titratable Acid or Base . 0.0001 meq/g
Residue after Evaporation . 0.001%
Solubility in water . To pass test
Water . 0.2%
RT 16930   1qt 12.00 Add to Cart
RT 16931   4x1qt 42.00 Add to Cart

arrow12JB-4(TM) Embedding Kit

Please see listingin Embedding Media Kits.

arrow12Karnovsky's Fixative

Please see Paraformaldehyde-Glutaraldehyde Solution.

Technical Tip

Elimination of Section Contamination During Staining

msdsarrowKinyoun's Solution

Kinyoun's carbol fuchsin staining solution for acid-fast bacteria.

RT 26087-05 Kinyoun's Solution 500 ml 57.00 Add to Cart
RT 26087-06 Kinyoun's Solution 1 L 93.00 Add to Cart

msdsarrow12Lanthanum Nitrate, Hexahydrate, Reagent

La(NO3)3·6H20   F.W. 433.03   CAS #10277-43-7

m.p. 40°C
Assay Min 99.9%
Calcium 0.001%
Magnesium 0.0001%

A metal stain, intracellular tracer.

  • Lane, N,J. and Treherne, J.E. (1970). Lanthanum staining of neurotubules in axons from cockroach ganglia. J. Cell Sci., 7:217
  • Lane, N.J. (1972) Fine structure of a lipidopteran nervous system and its accessibility to peroxidase and lanthanum. Z. Zellforsch., 131:205
RT 17300 50g 96.00 Add to Cart

msdsarrow12Lead Acetate, Trihydrate Reagent, A.C.S.

Pb(CH3COO)2·3H20   F.W. 379.33   CAS #6080-56-4
A.C.S. Specifications:

Assay . 99.0-103.0%
Chloride . 5ppm
Copper . 0.002%
Insoluble Matter .  0.01%
Iron . 0.001%
Nitrate and Nitrite (as NO3) . To pass test
0-5°C 17600 100g 22.00 Add to Cart
0-5°C 17600-25 25g 8.00 Add to Cart

*We are now offering Lead Acetate in 25 ml bottles to avoid its classification as a hazardous chemical for shipping purposes.

msdsarrow12Lead Citrate, Trihydrate

Pb(C6H5O7)2·3H2O   FW 1053.82   CAS #512-26-5
Minimum assay 99.%
The most widely used metal stain for ultra thin sections.
Reynolds, E.S., J. Cell Bio., 17, 208 (1963)
Sato, T., J. Electron Micro., 116, 133 (1976)
Venable, J.H. and Coggeshall, R. (1965). A simplified lead citrate stain for use in electron microscopy. J. Cell Biol., 25:407
Famy, A. (1967). An extemporaneous lead citrate for electron microscopy. Proc. 25th Ann. EMSA Meet., p.148. Claitor's Pub. Division, Baton Rouge, LA.

RT 17800   25g 26.00 Add to Cart
RT 17810   100g 68.00 Add to Cart

Technical Tips

Handling of Lead Solutions
Lead Staining
Common Lead Stock Solutions
A Stable Lead Staining Solution

msdsarrow12Lead Nitrate, Reagent, A.C.S.

Pb(NO3)2   F.W. 331.21   CAS #10099-74-8

Minimum Assay 99.5%
Insoluble matter 0.005%
Reaction Not less than pH 3.5
Chloride 0.0005%
Calcium 0.01%
Copper 0.0005%
Iron 0.0005%
Potassium 0.002%
Sodium. 0.002%

A metal stain for ultra thin sections.
Sato, T., (1967) J. Electron Micro., 16, 733

RT 17900   25g 6.00 Add to Cart

msdsarrow12Light Green SF Yellowish, Certified, C.N. #DcL-30

(Acid Green 5; Light Green 2G, S or 2GN; Acid Green F; Fast Acid Green N)
C37H34N2Na2O9S3   F.W. 792.86   CAS #5141-20-8
Solubility: 20.35% Water; 4.0% Alcohol; 5.25% Cellosolve; 12.0% Glycol; 0.0% Xylene

RT 17920   10 g 15.00 Add to Cart

msdsarrowLight Green SF Yellowish Solution

Light SF Yellowish, 0.2% in 0.2% acetic acid fo use in the GM Method, as a fungal stain.

RT 26085-05 Light Green SF Yellowish,
0.2% in 0.2% Acetic Acid
500 ml 32.00 Add to Cart
RT 26085-06 Light Green SF Yellowish,
0.2% in 0.2% Acetic Acid
1 L 61.00 Add to Cart

technical data sheetarrow12(SPURR), Low Viscosity, Embedding Kit

Please see listing in Embedding Media Kits.

RT 14300 Low viscosity kit (Dr. Spurr) msds
msds
msds
msds
kit 68.00 Add to Cart

arrow12Technical Tips

Embedding Resin Stain Permability

technical data sheetarrow12Lowicryl, Embedding Kit

Please see listing in Embedding Media Kits

RT 14330 Lowicryl K4M msds
msds
msds
kit 530.00 Add to Cart
RT 14340 Lowicryl HM20 msds
msds
msds
kit 530.00 Add to Cart
RT 14350 Lowicryl K11M msds
msds
msds
kit 530.00 Add to Cart
RT 14360 Lowicryl HM23 msds
msds
msds
msds
kit 530.00 Add to Cart

Technical Data Sheets and Newsletter

msdsmsds arrow12Lowicryl MonoStep Single Component Embedding Media

The same as our Lowicryl complete kits but this new formulation allows you one stop embedding without all of the cumbersome preparation and mixing. This pre-mixed, ready to use resin, saves you time and minimizes chemical contact. MonoStep Lowicryl is ideal for use in immunohistochemistry and immunolabeling. Store at room temperature.

RT 14335 Lowicryl K4m Polar
Embedding Medium; MonoStep
225g 236.00 Add to Cart
RT 14345 Lowicryl HM-20 Polar
Embedding Medium; MonoStep
225g 236.00 Add to Cart

arrow12LR White And LR Gold Resin

Please see listingin Embedding Media Kits.

Articles of Interest

Flat Embedding with LR-White

msdsarrow12Lucifer Yellow, CH, Dilithium Salt

C13H9Li2N2O9S2   F.W. 457.25   CAS #67769-47-5

A technique producing an electron dense osmiophilic polymer when Lucifer Yellow is injected into cells. Lucifer Yellow also can be viewed in living cells as a dye marker and is more sensitive than any other dye.
Cell Tissue Res., 204, 37 (1979) J. Histochem Cystochem, 30, 189 (1982) Science, 217, 953 (1982); 215, 1641 (1982); 214, 801 (1981); 225, 851 (1984); Cell, 14, 741 (1978). A fluorescent dye. Nature 292, 17 (1981)

0-5°C 17950   25mg 130.00 Add to Cart

msdsarrowLugol's Iodine

For use as a bacterial, fungal and inclusion body stain.

RT 26055-05 Lugol's Iodine Solution 500 ml 25.00 Add to Cart
RT 26055-06 Lugol's Iodine Solution 1L 44.00 Add to Cart

msdsarrow12Magnesium Chloride, Hexahydrate, Reagent, A.C.S.

MgCl2·6H2O   F.W. 203.30   CAS #7791-18-6
Assay: 99.0-102.0%

RT 18010   100g 22.00 Add to Cart

msdsarrow12Malachite Green 4, Certified C.N.#DcMg-11

Please see Basic Green Oxalate

RT 18100 25g 42.00 Add to Cart

msdsarrow12Maleic Acid, Crystalline, Repurified, A.C.S.

(Toxilic Acid; cis-Butenediodic Acid)
HOOCCH:CHCOOH   F.W. 116.08   CAS #110-16-7
m.p. 132-134°C
Assay 99.5%

Insoluble Matter 0.005%
Fumaric Acid 0.15%
Chloride 0.01%
Sulfate. 0.001%
RT 18150   250g 39.00 Add to Cart

msdsarrow12Mammalian Ringer Solution

Ingredients: NaCl; KCl; CaCl2·6H2O; NaHCO3; MgCl2·6H2O; Glucose and Distilled water.

RT 11763-05 Mammalian Ringer Solution 500 ml 24.00 Add to Cart
RT 11763-10 Mammalian Ringer Solution 1 L 32.00 Add to Cart

msdsarrowMay-Grunwald Staining Solution

A ready-to-use stain for bone marrow staining.

RT 26064-05 May-Grunwald Stain Solution 500 ml 31.00 Add to Cart
RT 26064-06 May-Grunwald Stain Solution 1 L 56.00 Add to Cart

msdsarrowMayer's Hematoxylin

Stain selectivity for nuclei. Ready-to-use.

RT 26043-05 Mayer's Hematoxylin 500 ml 40.00 Add to Cart
RT 26043-06 Mayer's Hematoxylin 1 L 78.00 Add to Cart
RT 26043-10 Mayer's Hematoxylin 4 L 282.00 Add to Cart

arrowModified Mayer's Hematoxylin

Stock Solution. Ready-to-use.
Ingredients: Hematoxylin 0.4%, Ammonium Aluminum Sulfate 0.5%,
Sodium Iodate: 0.03%, Citric Acid 0.1%, Chloral Hydrate 5%

RT 26043-18 Modified Mayer's Hematoxylin 500 ml 45.00 Add to Cart

msdsarrow12Methacrylic Acid, Glacial

(2-Methylpropenoic Acid)
CH2:C(CH3)COOH   F.W. 86.09   CAS #79-41-4
Assay: >98%
Stab. with 0.25% Hydroquinone monomethyl ether

RT 18500   450 ml 39.50 Add to Cart

msdsarrowMethanol, Reagent, A.C.S.

(Methyl Alcohol)
CH3OH   F.W. 32.04   CAS #67-56-1
Assay: 99.8%
Specific Gravity : 0.79
A.C.S. Specifications:

Residue after Evaporation . 0.001%
Boiling Range (Boiling point 64.6°C) . 2°C max
Appearance . Clear
Color (A.P.H.A.) . 10 max
Acetone & Aldehydes (0.001%) . To pass test
Titratable Acid . 0.0003 meq/g
Titratable Base . 0.0002 meq/g
Water . 0.2% max
RT 18510   1qt 15.00 Add to Cart
RT 18511   4x1qt 54.00 Add to Cart

msds arrowMethenamine

Please see Hexamethylenetetramine

RT 16710   50g 14.00 Add to Cart

msdsarrowMethenamine Solution

A stock solution for use in bacterial and fungal stains (Grocott's Method/GMS).

RT 26083-05 Methenamine, 3% Aqueous Solution 500 ml  25.00 Add to Cart
RT 26083-06 Methenamine, 3% Aqueous Solution  1 L 45.00 Add to Cart

msdsarrow122-Methyl Butane, Reagent

(Isopropentane)
CH2H5CH(CH3)2   F.W. 72.15   CAS #78-78-4
b.p. 27-31°C
Specific Gravity @ 20°C: 0.62

RT 18550   450 ml 43.00 Add to Cart

msdsarrow12Methyl Cellulose, 400 cPS,USP/NF

(Methylcel MC; Cellulose Methyl Ether)
CAS #9004-67-5
Methoxyl (-CH3) content : 27.5-31.5%
Medium viscosity (2% in water, @20°C): 230-550 cps
Used with polyethylene glycol in the presence of acidic uranyl acetate to stain cryo sections.

RT 18560   100g 50.00 Add to Cart

msdsarrow12Methylene Blue Chloride, Certified, C.N. #DcA-24

(Basic Blue 9; Swiss Blue)
C16H18N3SCl •3H2O  F.W. 319.86+aq   CAS #7220-79-3
Solubility: 9.5% Water; 6.0% Alcohol; 6.5% Cellosolve; 10.0% Glycol; 0.0% Xylene.

  • Used to distinguish autonomic nerves. Richardson, K.C., (1969) Anat. Rec., 164, 231. General stain for animals, histology.
  • A simple rapid staining procedure for methacrylate embedded tissue sections using chromotrope 2R and Methylene Blue. Dougherty, M.M., and King, J.S., (1984) Stain Tech., 59, 149
  • An established routine method for differential staining of epoxy-embedded tissue sections. Schroeder, H.E., Rossinsky, K., and Muller, W., (1980) Microsc. Acta 83, 111
  • Used with Basic Fuchsin for stain epoxy-embedded tissue. Sato, T. and Shamoto, M. (1973). A simple rapid polychrome stain for epoxy-embedded tissue. Stain Technol., 48:223
RT 18600   25g 38.00 Add to Cart

Technical Tips

A Methylene Blue-Azure II Stain

arrow12Methylene Blue Solutions

Please see the listing in Prepared Staining Solutions

msdsarrow12Methyl Ethyl Ketone, Reagent

(2-Butanone)
CH3COCH2CH3   F.W. 72.11   CAS #78-93-3

Assay >98%
Specific Gravity (H2O=1) : 0.799-0.802
Acidity (as CH3COOH) 0.003%
Water 0.2%
RT 18650   450 ml 30.00 Add to Cart

msdsarrow12Methylene Chloride, Reagent, A.C.S.

(Dichloromethane; Plastisolve)
CH2Cl2   F.W. 84.93   CAS #75-09-2

RT 19600   1qt 32.00 Add to Cart
RT 19610   4x1qt 126.00 Add to Cart

arrow12Technical Tips

Storage of Epoxy Resins

msdsarrow12Methyl Green, Certified, C.N. #DcG-10

(Zinc Chloride Salt; Ethyl Green)
C27H35N3C12·ZnC12   F.W. 608.79   CAS #7114-03-6
Solubility: 8.0% water; 3.0% Alcohol; 1.8% Cellosolve; 3.5% Glycol; 0.0% Xylene.

  • Used as a counterstain in Almann's aniline acid fuchsin technique for mitochrondria; used in Kurnick's Methyl Green-Pyonin stain for nuclei and cystoplasmic granules of liver cells. Marker for DNA at acid and neutral conditions. J.B. Boyd, H.R. Mitchell, (1965) Anal. Biochem., 13, 28.
  • Used for epoxy-embedded thick sections. Sievers, J. (1971).
  • Basic two-dye stains for epoxy-embedded 0.3-1 micron sections. Stain Technol., 46:195
RT 18700   25g 56.00 Add to Cart

msdsarrow12Methyl Methacrylate, Monomer

(Methacrylic Acid Methyl Ester)
CH2·C(CH3)COOCH3   F.W. 100.12   CAS #80-62-6
b.p. 99-100°C
Specific Gravity @20°C : 0.943
Inhibitor (Hydroquinone): 0.0025% (25ppm)

0-5°C 18800   225 ml 9.00 Add to Cart

arrow12Methyl Methacrylate/Butyl Methacrylate Embedding Kit,

Please see listing inEmbedding Media Kits

msdsarrow12Methyl Violet 2B, Certified, C.N. #DcMv-5

(Basic Violet 1; Dahlia B; Gentian Violet; Paris Violet; Pyoktanium Coeruleum)
C24H28N4Cl   F.W. 393.96   CAS #8004-87-3
Solubility: 9.0% Water; 8.25% Alcohol; 9.0% Cellosolve; 8.5% Glycol;0.01% Xylene
Dye content: 75% min
Used with Methyl Green to stain Epoxy-embedded sections.

RT 18850   25g 42.00 Add to Cart

arrow12Michel's Fixative

Please see listing in Reagents for Histology.

arrow12Microbed Embedding Kit

Please see listing in Embedding Media Kits.

msdsarrow12Millonig's Phosphate Buffer

Please see listing in Prepared Buffers

0-5°C 11582-05   500 ml 21.00 Add to Cart
0-5°C 11582-10   1 lit 30.00 Add to Cart

msdsarrow12Molybdic Acid, Ammonium Salt

Please see ammonium molybdate

RT 10500   100g 37.00 Add to Cart

msdsarrow12MOPS, Biological Buffer

(3-(N-Morpholino) propanesulfonic acid)
C7H15NO4H   F.W.209.26   CAS #1132-61-2
Assay: >99.%

RT 18900   100g 69.00 Add to Cart

msdsmsdsarrowMucicarmine/Metanil

For use with hematoxylin and metanil yellow for the determination of carbohydrates and mucosubstances.

RT 26080-05 Mucicarmine Stock Solution 500 ml 57.00 Add to Cart
RT 26080-06 Mucicarmine Stock Solution 1 L 104.00 Add to Cart
RT 26081-05 Metanil, 0.25% Stock Solution
500 ml 26.00 Add to Cart
RT 26081-06 Metanil, 0.25% Stock Solution 1 L 45.00 Add to Cart

Certificates of Analysis


NMA to Orange arrow13arrow13