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Electron Microscopy Sciences

Chemicals for Microscopy

Certificates of Analysis

arrow13Pipes to Ruthenium

msdsarrow12Pipes Buffer Solution

Please see listing in Prepared Buffers

RT 11610   500 ml 30.00 Add to Cart

msds arrow12Pipes, 1.5 Sodium Salt, Crystal

[Piperazine-N,N-bis (2-ethanesulfonic acid,) 1.5 Sodium]
F.W. 335.3   pK = pH = 6.8 @25°C   CAS #10010-67-0

RT 19230   25g 19.00 Add to Cart
RT 19240   100g 66.00 Add to Cart

msdsarrow12Plastisolve

Please see Methylene Chloride

RT 19600   1qt 32.00 Add to Cart
RT 19610   4x1qt 126.00 Add to Cart

msdsarrowPlatinum Blue, an EM Stain

IBI Blue Platinum Stain Pt4N8H6O24C20

An EM stain that may have the ability to replace Uranyl Acetate in some instances. If you have issues with having Uranium salts that are radioactive (depleted or not) in the lab this may be the answer for you. An alternative for staining TEM thin sections

RT 22407 Platinum Blue 1ml 225.00 Add to Cart

arrow12Poly-L-Lysine Solutions

msds Poly-L-Lysine Hydrobromide 30,000-70,000

CAS #25988-63-0

A polycation, which binds to DNA, red cell membranes and any negatively, charged proteins. It is useful for promoting cell adhesion; for the preparation of polycationic beads, useful in immobilization techniques; immobilization of membranes; immobilization of plant protoplasts. In cell culture, immobilization by micro encapsulation with the alginate-poly-L-lysine microcapsule system.

We offer a prepared 0.1% aqueous solution, micro-filtered, which is ideal for cell culture growth.

RT 19320-A Poly-L-Lysine 0.1% Solution 10 ml 28.50 Add to Cart
RT 19321-A Poly-L-Lysine 0.1% Aqueous Solution 100 ml 280.00 Add to Cart
msdsB/ Poly-L-Lysine Hydrobromide 70,000-150,000

CAS #25988-63-0

Poly-L-lysine with a molecular weight of >70,000. This solution is useful in promoting cell adhesion to solid substrates. Used for nuclear antigen-coated red cells in hymolytic plaque assay.

We offer a prepared 0.1% aqueous solution which is micro-filtered and is used to increase tissue adhesion to the glass slide, which is needed for immunohistochemical and immunolabeling techniques, as well as lengthy staining procedures.

RT 19320-B Poly-L-Lysine 0.1% Solution 10 ml 14.00 Add to Cart
RT 19321-B Poly-L-Lysine 0.1% Aqueous Solution 100 ml 118.00 Add to Cart

msdsarrow12Polyester wax

A synthetic polyester wax with a very low melting point (37°C). Invented by Dr. H.F. Steedman, as a ribboning embedding medium. Its main advantage over paraffin and ester waxes is its low melting point, which reduces tissue hardening, shrinkage, and eliminates the need for infiltration ovens.

The wax is soluble in most organic solvents, including alcohol, ethers, esters, ketones and hydrocarbons. The wax is water tolerant, almost opaque, and sections easily. No electrification of ribbons occurs during sectioning. Sections of 2 microns and more may be cut at room temperatures between 10 and 22°C. It is advantageous to keep the wax in its solid state prior to use.

RT 19312 Polyester Wax 500g 125.00 Add to Cart

arrow12Polyethylene Glycol, Carbowax

Please see Carbowax.

msdsarrow12Polyvinyl Alcohol 15000

(CH2CHOH)n   F.W. 14000-15000   CAS #9002-89-5
Degree of Hydrolyzation: 86-89 mol%

  • A water-miscible embedding medium: Munoz-Guerra,S. and Subrirana, J.A. (1982)
  • A crosslinked polyvinyl alcohol: a water-miscible polymer used as an embedding medium for electron microscopy. Mikroskopie, 39:346
RT 19800   500g 150.00 Add to Cart

arrow12Ponceau B.S.

See Biebrich Scarlet

msdsarrow12Potassium Bromide, Reagent, A.C.S.

KBr   F.W. 119.00   CAS #7758-02-3
Specifications:

Insoluble Matters 0.005%
Bromate To Pass Test
Chloride .0.2%
Iodide 0.001%
Nitrogen 0.005%
Sulfate 0.005%
Barium 0.002%
Heavy Metals 0.0005%
Sodium 0.02%

Used in the preparation for autoradiography

RT 20040   100g 37.00 Add to Cart

msdsarrow12Potassium Chloride, Crystal, Reagent, A.C.S.

KCl   F.W. 74.55   CAS #7447-40-7 
Specifications:

Insoluble Matters 0.005%
Iodide 0.002%
Bromide 0.01%
Chlorate and Nitrate 0.003%
Sulfate. 0.001%
Phosphate 0.0005%
Sodium 0.005%
RT 20060   100g 12.00 Add to Cart

msdsarrow12Potassium Dichromate, Crystal, Reagent, A.C.S.

K2Cr2O7   F.W. 294.18   CAS #7778-50-9
A.C.S. Specifications:

Insoluble and NH4OH ppt. 0.005%
Chloride 0.001%
Sulfate 0.005%
Calcium 0.003%
Sodium 0.02%
Loss on drying at 105°C 0.05%
RT 20100   250g 29.50 Add to Cart

arrow12Potassium Dichromate Solutions

Please see listing in Reagents for Histology.

msdsarrow12Potassium Ferricyanide, Reagent, A.C.S.

K3Fe(CN)6   F.W. 329.25   CAS #13746-66-2
A.C.S. Specifications:

Chloride 0.01%
Ferro Compounds (Fe(Cn)4) 0.05%
Insoluble Matters. 0.005%
Sulfate 0.01%
RT 20150   100g 22.00 Add to Cart

msdsarrow12Potassium Hydroxide, Pellets, Reagent, ACS.

KOH   F.W. 56.11   CAS #1310-58-3
Assay>85.0%
Water contains: 10-15%

RT 20155   100g 16.00 Add to Cart

msdsarrow12Potassium Iodide, Granular, Reagent, A.C.S.

KI   F.W. 166.00   CAS #7681-11-0
Refer to Bismuth Carbonate for references on use.

RT 20158   50g 48.00 Add to Cart

msdsarrow12Potassium Oxalate, Reagent, A.C.S.

(CO2K)2·H2O   F.W. 184.23   CAS #6487-48-5
Assay: 98.5-101.0%
Neutrality: To Pass Test

Specifications:  
Insoluble Matters 0.01%
Chloride 0.002%
Sulfate 0.01%
Ammonium 0.002%
Heavy Metal 0.0025%
Iron 0.001%
Sodium .0.02%
RT 20160   100g 19.00 Add to Cart
RT 20162   500g 82.00 Add to Cart

msdsarrow12Potassium Permanganate, Reagent, A.C.S.

KMnO4   F.W. 158.03   CAS #7722-64-7
A.C.S. Specifications:

Chloride & Chlorate 0.005%
Nitrogen Compounds 0.005%
Sulfate .0.02%
Insoluble Matters 0.2%

An EM fixative and a metal stain. J. Ultrastruct. Res., 21,424 (1968)

RT 20200   250g 36.00 Add to Cart

arrow12Potassium Permanganate Solutions

Please see listing in Reagents for Histology.

msdsarrow12Potassium Phosphate, Monobasic, Reagent, A.C.S.

KH2PO4   F.W. 136.09   CAS #7778-77-0
A.C.S. Specifications:

Insls. and Calcium, and NH4OH ppt 0.01%
Loss on drying over H2SO4 0.2%
pH of 5% Solution @ 25°C 4.1-4.5
Chloride 0.001%
Nitrogen Compounds 0.001%
Sulfate 0.003%
Sodium 0.005%
Iron 0.002%
RT 20250   500g 32.00 Add to Cart

msdsarrow12Potassium Pyroantimonate, Trihydrate Potassium antimonate

KSbO3·3H2O   F.W. 262.90   CAS #12208-13-8
Minimum assay: 94%
Minimum sensitivity to Na: 1:430
Insoluble matternot more than 0.01%
*Ultrastructural localization sodium. J. Histochem Cystochem., 24, 740 (1976)

RT 20220   25g 26.00 Add to Cart

msdsarrow12Potassium Sodium Tartrate, Tetrahydrate Reagent, A.C.S.

(Sodium Potassium Tartrate)
KNaC4H4O6·4H2O   F.W. 282.22   CAS #6381-59-5
Assay 99.0-102.0%
Water 21.0-27.0%
Used with bismuth subnitrate for staining polysaccharides, including glycogen without oxidizing agents.

  • Shinji, Y., et al (1975). A new electron microscopic histo-cyto chemical staining method: demonstration of glycogen particles. Acta Histochem., Cytochem., 8:139
RT 20260   100g 27.00 Add to Cart

msdsarrow12Pronase Reagent, Ready To Use

Description: Pronase is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: Ready-to-use Pronase reagent available in 2 sizes: 15 and 100 ml.
4-8°C 64142-03 Pronase Reagent 15 ml 75.00 Add to Cart
4-8°C 64142-04 Pronase Reagent 100 ml 145.00 Add to Cart

arrow122-Propanol, Propyl Alcohol

Please see Iso-propyl Alcohol.

msdsarrow12Propylene Glycol, Reagent, A.C.S.

(1,2-Propanediol)
CH3CHOHCH2OH   F.W. 76.10   CAS #57-55-6
Specific Gravity (H2O=1): 1.04
Assay>99.5%

Specifications:

Residue after Ignition 0.005%
Chloride 1ppm
Water 0.2%
Titratable Acid 0.0005meq/g
RT 20350   450 ml 27.00 Add to Cart
RT 20352   4x450 ml 94.00 Add to Cart

arrow12msdsPropylene Oxide, EM Grade

(1,2-Epoxypropane, Methyloxirane)
CH3CHCH2O   F.W. 58.08   CAS #75-56-9
m.p. -112°C; b.p. 34°C; f.p. -37°C
Vapor pressure @ 33°C: 980 mbar
Specifications:

Specific Gravity @ H2O=1 .0.831
Acidity, Wt. ppm as Acetic Acid 2
Aldehydes, ppm as Propionaldehyde 3
Water,wt. ppm 35
Total Chloride, wt. ppm as Cl 1
Nonvolatile matter, g/100 ml max 0.001
Color, Platinum-Cobalt .0

A solvent agent for epoxy resins. Used widely for infiltration and gradual replacement of the dehydration agent.

RT 20401 Metal can* 450 ml 29.00 Add to Cart
RT 20411 Metal can* 4x450 ml 104.00 Add to Cart
RT 20412 Metal can* 250 ml 26.00 Add to Cart
RT 20414 Metal can* 4x250 ml 93.00 Add to Cart
* Also availables in glass bottles. Please contact us to request.

msdsarrow12Pyronin Y, Certified, C.N. #DcPy-2

(Pyronin G)
C17H19N2OCl  F.W. 302.81  CAS #92-32-0
Solubility: 9.0% Water; 0.5% Alcohol; 1.85% Cellosolve; 4.35% Glycol; 0.0% Xylene

RT 19560   5 g 51.00 Add to Cart

msdsarrow12Quetol 651, Resin

(Ethylene Glycol Diglycidyl Ether)
C8H14O4   F.W. 174.20   CAS #2224-15-9
Specific Gravity (H2O=1) : 1.15

0-5°C 20440   225 ml 110.00 Add to Cart

technical data sheetarrow12Quetol 651, NSA Kit

Please see listing in Embedding Media Kits.

0-5°C 14640   kit 160.00 Add to Cart

arrow12Replicating Materials

Please see the complete listing in the Materials Science section.

msdsarrow12Ruthenium Red, 37.5% Ru

[(NH3)5RuORu(NH3)4ORu(NH3)5]Cl6·4H2O   F.W.858.42   CAS #11103-72-3

  • Stain for monopolysaccharides and tight junctions. Luft, J.T., Proc. 6th Int. Cong. Em (1966); J. Cell. Biol., 57, 874 (1973)
  • Stain for pectin and for diverse cell surfaces. J. Cell Biol.,23, 54A (1964)
  • "Positive Staining for EM" van Nostrand Reinhold Co., New York, NY (1975) pp 163-165
  • Ruthenium Red and Violet II. Fine structural localization in animal tissues. Luft J.H. Anat. Rec., 171:369, (1971a)
  • Structure and staining characteristics of myofiber external lamina. Zacks, S.I. et al. J. Histochem. Cytochem., 21:703 (1973)
  • Glycoaminoglycans in developing chick-embryo aorta revealed by ruthenium red: an electron microscopy study. Kadar, A. et al. J. Pathol., 108:275 (1973)
  • Uptake of marker particles by in vitro ventilated and perfused rat lung. Vidic, B. Am. J. Anat., 138:521 (1973)
RT 20600   1g 95.00 Add to Cart

msdsarrow12Ruthenium Tetroxide, 0.5% Stabilized Aqueous Solution

In Crystal form: RuO4   F.W. 165.70   CAS #20427-56-96
m.p. 25.4°C b.p. 40°C
Ruthenium tetroxide is very similar to Osmium tetroxide, and is used as a fixation/staining agent for electron microscopy. Ruthenium tetroxide offers excellent staining of saturated and unsaturated polymer materials as well as stabilizing thin sections under the bombardment of the electron beam.
NOTE: Penetration of ruthenium tetroxide into the tissue is poor.

  • Caughey, R.C. and Miller, M.A., Proceed. of 44th Ann. Mtg. EMSA (1986), page 256
  • Hayat, M.A., Basic Technique for Electron Microscopy, Academic Press, NY, 1986, p 22
  • Trent, J.S., Macromolecules, 17, 2930 (1984); 16, 539 (1983)
RT 20700-05   Box 5x10 ml 269.00 Add to Cart

Certificates of Analysis


Safranin to Sym-Collidine arrow13arrow13