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Electron Microscopy Sciences

Chemicals for Microscopy

Certificates of Analysis

arrow14Safranin to Sym-Collidine

msdsSafranin O, Certified, C.N. #DcS-25

(Safranin T, Y or A; Basis Red 2; Cotton Red; Gossypimine
C20H19N4Cl   F.W. 350.85   CAS #477-73-6
Solubility: 4.5% Water; 3.5% Alcohol; 5.0% Cellosolve; 3.5% Glycol; 0.0% Xylene

RT 20800   25 g 30.00 Add to Cart

msdsAlcoholic Stain SolutionsSchiff’s Reagent

For use in the Periodic Acid Leucofuchsin (PAS) method as a general tissue stain.

0-5°C 26052-05 Schiff’s Reagent 500 ml 43.00 Add to Cart
0-5°C 26052-06 Schiff’s Reagent 1L 70.00 Add to Cart

msdsScott’s Tap Water Substitute

Magnesium sulfate buffered with sodium bicarbonate. Readyto- use.

RT 26070-06 Scott’s Tap Water Substitute 1 L 31.00 Add to Cart
RT 26070-07 Scott’s Tap Water Substitute 4 L 96.00 Add to Cart

arrow12Section Block

A new class of blocking solutions based on chemically modified and fragmented ultra-pure casein. Effectively reduces unwanted binding of primary antibody and conjugates you use to charged surface of the slide and tissue section. Greatly reduces non-specific binding while preserving the specific reaction, by saturating potential non-specific protein-protein interactions. Moreover, in contrast to BSA-based, IgGm casein or serum - based blocking solutions there is no interaction of specific antibody and blocking protein itself. or oth Is not comparable to other commercially available or home-made blocking solutions. Recommended for research and diagnostic pathology, especially for retrieved sections and polyclonal antibodies.

4-8°C 62710 Section Block 50 ml 150.00 Add to Cart
4-8°C 62711 Section Block 125 ml 265.00 Add to Cart
4-8°C 62712 Section Block 500 ml 995.00 Add to Cart

Silver, Collodial

Please see our complete listing.

msdsSilver Lactate, Powder

CH3CH(OH)CO2Ag   F.W. 196.94  CAS #128-00-7
Silver content: approx. 90% min.
* To localize intracellular anions (Cl-). Komnick, (1962).
* Elektronemikrokopische Lokalisation von Na+ und Cl in Zellan und Geweban. Protoplasma, 55,414.

RT 21000   5g 35.00 Add to Cart

msdsSilver Protein, Strong

Protargol, 8.3% Ag  CAS #9008-42-8
Thiery, (1967). See Silver Nitrate

RT 21100   5g 87.50 Add to Cart

msdsSilver Nitrate, Reagent, A.C.S.

AgNO3   F.W. 169.89  CAS #7761-88-8
Assay 99.9+%
A.C.S. Specifications:

Substances not precipitated by HCl-max 0.010%
Sulfate max 0.0002%
Copper max 0.0002%
Iron max 0.0002%
Lead max 0.001%

References for Silver stain:

  • Swift, J.A. (1968). The electron histochemistry of cystine-containing proteins in thin transverse sections of human hair. J.R. Microsc. Soc. 88,449.
  • Thiery, J.P. (1967). Mise en evidence des Polysaccharides sur coupes fines en microscopie eletronique. J. Microsc. (Paris) 6,987.
  • Rambourg, A. (1967). An improved silver methenamine technique for the detection of periodic acid-reactive complex carbohydrates with the electron microscope.J. Histochem. Cytochem. 15, 409.
  • Hermandez et al., (1968)). Periodic acid-chromic acid-methenamine silver technique for glycoprotein detection in the electron microscope. J. Histochem. Cystochem. 16, 507.
  • Ribi, W.A. (1976). A golgi-electron microscope method for insect nervous tissue. Stain Technol. 51,13.
  • Braak, H., and Braak, E. (1982). A simple procedure for electron microscopy of Golgi-impregnated nerve cells. Neurosci. Lett. 32,1.
RT 21050   25g 70.00 Add to Cart
RT 21052   100g 270.00 Add to Cart

arrow12Silver Nitrate Solutions

Please see listing in Reagents for Histology

msds(S-TPPS) Silver Tetraphenylporphin Sulfonate

An EM stain for elastin. Albert, E.N., And Fleischer E. (1970).
A new electron dense stain for elastic tissue. J. Histochem.Cystochem. 18, 697

0-5°C 21110   100mg 50.00 Add to Cart

msdsSodium Acetate,Trihydrate Reagent, A.C.S.

CH3COONa·3H2O   F.W. 136.08   CAS #6131-90-4
A.C.S. Specifications:

Assay 99.0-100.5%
Ca, Mg and R2O3 ppt 0.01%
Chloride 0.001%
Insoluble Matter- 0.005%
Iron, Heavy metal, Phosphate 5ppm/ea
Sulfate 0.002%
Potassium 0.005%
pH of 5% sol. @ 25°C 7.5-9.2
RT 21120   500g 48.00 Add to Cart

msds Sodium Bicarbonate, Powder, Reagent, A.C.S.

NaHCO3   F.W. 84.01   CAS #144-55-8
Assay 97.7-100.3%
Specifications:

Insoluble Matters .0.015%
Chloride 0.003%
Phosphate 0.001%
Sulfur Compounds 0.003%
Ammonium 5ppm
Iron 0.001%
Potassium 0.05%
RT 21125   500g 21.00 Add to Cart

msdsSodium Bisulfite Solution

A 1% aqueous stock solution for fungal staining.

RT 26084-05 Sodium Bisulfite Solution 500 ml 19.00 Add to Cart
RT 26084-06 Sodium Bisulfite Solution 1 L 33.00 Add to Cart

Sodium Borate, Tetra, Granular, Reagent, A.C.S

Please see Borax Tetra.

msdsSodium Cacodylate Trihydrate Sodium dimethyl arsenate,

Please see Cacodylic Acid, Sodium Salt .

RT 12300   100g 99.50 Add to Cart
RT 12310   500g 425.00 Add to Cart

msds Sodium Cacodylate, Buffer Solution

Please see listing in Prepared Buffers.

RT 11650   225 ml 28.00 Add to Cart
RT 11652   500 ml 40.00 Add to Cart

msdsSodium Carbonate, Anhydrous, Reagent, A.C.S.

Na2CO3   F.W. 105.99   CAS #497-19-8
Specifications:

Insoluble Matters 0.01%
Loss on Heating at 280°C 1.0%
Chloride 0.001%
Phosphate. 0.001%
Nitrogen Compounds 0.001%
Sulfur Compounds. 0.003%
Ammonium Hydroxide ppt. 0.01%
Arsenic. 1ppm
Ca and Mg 0.01%
Heavy Metals 5ppm
Iron 5ppm
Potassium 0.005%
Silica (SiO2) 0.005%
RT 21135   500g 50.00 Add to Cart

msdsSodium Citrate, Granular, Reagent

Na3C6H5O7·2H2O   F.W. 294.11   CAS #6132-04-3

Assay 99.9%
Insoluble Matters 0.005%
Free Acid (as Citric) 0.02%
Free Alkali 0.05%
Chloride 0.003%
Sulfate 0.005%
Calcium 0.005%
Heavy Metals .3ppm
Iron 0.001%
RT 21140   500g 14.00 Add to Cart

msdsSodium Ethylate

(Sodium Ethoxide)
C2H5ONa   F.W. 68.05   CAS #141-52-6
Used as an agent for flattening thick sections (0.5-2.0 micron) of epoxy embedded material. Felix Sierra, Christa Hegele-Hartung and Henning M. Beier (1990).
An improved technique for flattening semithin sections of epoxy-embedded tissues used for high resolution light microscopy. J. of Microscopy Vol 159, Pt 1, (July 1990), pp. 95-98

RT 21150   25g 10.00 Add to Cart

Technical Tips

An Improved Technique for Flattening Semithin Sections of Epoxy-Embedded Tissues Used for High-Resolution Light Microscopy

msds Sodium Hydroxide, Pellets, Reagent, A.C.S.

NaOH   F.W.40.00   CAS #1310-73-2
Assay >97.0%
Specifications:

Sodium Carbonate 1%
Nitrogen Compounds 0.001%
Chloride 0.005%
Phosphate 0.001%
Sulfate 0.003%
Heavy Metals 0.002%
Ammonium Hydroxide ppt 0.02%
Iron 0.001%
Potassium 0.02%
Nickel 0.001%
Mercury 0.1ppm
RT 21160   100g 8.00 Add to Cart
RT 21162   500g 20.00 Add to Cart

msdsmsdsSodium Hydroxide, Solution

2N Standardized   CAS #1310-73-2
Carbonate-free. This solution is prepared from ACS Reagent grade sodium hydroxide, and is standardized colormetrically against potassium biphthalate primary reference material.
Normal Concentration 1.98-2.02

RT 21170 Sodium Hydroxide 2N Solution 225 ml 13.00 Add to Cart
RT 21170-01 Sodium Hydroxide 1N Solution 225 ml 12.00 Add to Cart

msds Sodium Methoxide, Anhydrous

(Sodium Methylate)
CH3ONa   F.W. 54.03   CAS #124-41-4
Used in the removal of resin from sections.

RT 21200   20g 12.00 Add to Cart

msdsSodium Phosphate, Dibasic, Heptahydrate, Reagent, A.C.S.

Na2HPO4·7H2O   F.W. 268.07   CAS #7782-85-6

Assay 98.0-102.0%
Loss on Drying @ 105°C 43-50%
pH of a 5% solution @25°C 8.7-9.3
Specifications:
Insoluble Matters <0.005%
Chloride 0.001%
Nitrogen Compounds 0.001%
Sulfate 0.005%
Arsenic 0.0005%
Heavy Metals 0.001%
Iron 0.001%
RT 21180   500g 20.00 Add to Cart
RT 21182   1 kg 30.00 Add to Cart

msdsSodium Phosphate, Monobasic,

Hydrate,Reagent, A.C.S.
NaH2PO4·H2O   F.W. 137.99   CAS #10049-21-5
Assay 98.0-102%
pH of a 5% solution @25°C-4.1-4.5
Specifications:

Insoluble Matters- <0.01%
Chloride 5ppm
Nitrogen Compounds 0.001%
Sulfate. 0.003%
Arsenic. 0.5ppm
Heavy Metals 0.001%
Iron 0.001%
RT 21190   500g 30.00 Add to Cart
RT 21192   1 kg 56.00 Add to Cart

Technical Tips

Temperature Conversions

msdsSodium Sulfite, Reagent, A.C.S.

Na2SO3   F.W. 126.04   CAS #7757-83-7
Assay >98.0%
Specifications:

Insoluble Matters 0.005%
Free Acid To Pass Test
Titratable Free Base 0.03meq/g
Chloride .0.02%
Arsenic 0.0001%
Heavy Metals 0.001%
Iron 0.001%
RT 21300   1 kg 26.00 Add to Cart

msdsSodium Tartrate, Reagent, A.C.S.

Na2C4H4O6·2H2O   F.W. 230.08   CAS #6106-24-7
Assay 99.5%-101.0%
pH of a 5% solution @25°C-7.0-9.0
Loss on Drying @150°C-15.61-15.71%

Specifications:

Insoluble Matters 0.005%
Chloride 0.0005%
Phosphate 0.0005%
Sulfate 0.005%
Ammonium 0.003%
Calcium 0.01%
Heavy Metals 0.0005%
Iron 0.001%
RT 21350   25 g 12.50 Add to Cart

msdsSodium Tungstate, Reagent, A.C.S.

Na2WO4.2H2O   FW 329.86   CAS #10213-10-2
Assay 99.0-101.0%
Specifications:

Insoluble Matters 0.01%
Chloride 0.005%
Molybdenum 0.001%
Nitrogen 0.001%
Sulfate 0.01%
Arsenic 0.0005%
Heavy Metals 0.001%
  • Stockert,J.C. (1977). Sodium Tungstate as a stain in electron microscopy. Biol. Cellul., 29:211
  • Takeuchi, I.K. (1981). Differential staining of nucleoli and chromatin by sodium tungstate. J. Electron Microsc., 30:15
RT 21400   25g 14.00 Add to Cart

msdsSorensen's Phosphate Buffer

Please see listing in Prepared Buffers

0-5°C 11682-05   500 ml 23.00 Add to Cart
0-5°C 11682-10   1L 30.00 Add to Cart

technical data sheet(SPURR), Low Viscosity Embedding Kit

Please see listing in Embedding Media Kits.

RT 14300 Low viscosity kit (Dr. Spurr) msds
msds
msds
msds
kit 68.00 Add to Cart

Staining Solutions

We now offer a complete line of prepared, ready-to-use, high quality staining solutions for all standard staining procedures used by the Biological Staining Commission and the Armed Forces Institute of Pathology.

  • All solutions and concentrations are packaged and prepared in convenient and precise quantities
  • Premixed ready-to-use; saves you time and effort
  • Consistently uniform results are obtained.

For a complete list and description of all of our stains, please see Chemicals for Microscopy

For Prepared Staining Kits, please see the complete listing of Prepared Staining Solutions

Technical Tips

Improving the Adhesion of Sections Filmless Grids

msdsStyrene, Reagent

(Vinylbenzene)
C6H5CH:CH2   F.W. 104.14   CAS #100-42-5
Specific Gravity (H2O=1): 0.909
Assay >99.0%
Stabilizer (p-tert-Butylcatechol)-10-15ppm
A monomeric styrene used as an embedding media for ultramicrotomy.

  • DeLamater, E.D., et al (1971). The use of styrenes as embedding media for electron microscopy. Pro. 29th Ann. Meet. EMSA, p. 488. Claitor's Pub. Divsion, Baton Rouge, LA
  • Kushida, H. (1962b). On ultraviolet polymerization of styrene resins in embedding for electron microscopy. J. Electron Microsc., Vol. 2. p.10. Academic Press, New York.
  • Kushida, H. (1961a). A styrene-methacrylate resin embedding method for ultrathin sectioning. J. Electron Micro., 10:16
RT 21500   450 ml 11.00 Add to Cart

msdstechnical data sheetStyrene Embedding Kit

Please see listing in Embedding Media Kits.

RT 14650   kit 38.00 Add to Cart

msdsSuccinic Acid, Crystal, Reagent, A.C.S.

(CH3COOH)2   F.W. 118.09   CAS #110-15-6
m.p. 185-191°C
Assay >99.0%
Specifications:

Insoluble Matters- .0.01%
Chloride 0.001%
Sulfate 0.003%
Phosphate 0.001%
Nitrogen Compound 0.001%
Heavy Metals 5ppm
Iron 5ppm
RT 21550   100g 25.00 Add to Cart

msdsSucrose, Reagent, A.C.S.

C12H22O11   F.W. 342.20   CAS #57-50-1
Specifications:

Insoluble Matters 0.005%
Loss on Drying 105°C 0.03%
Residue after Ignition 0.01%
Titratable acid 0.0008meq/g
Chloride 0.005%
Sulfate 0.005%
Heavy Metals- 5ppm
Iron 5ppm
Invert Sugar 0.05%
RT 21600   500g 22.00 Add to Cart

msdsSudan Black B, Certified, C.N. #DcZb-2

(Solvent Black 3)
C29H24N6   M.W. 456.56   CAS #4197-25-5
Solubility: Water-insoluble; Alcohol-soluble; 4.0% Cellosolve; 1.0% Glycol.

RT 21610   25 g 27.00 Add to Cart

msdstechnical data sheetemsexclu.gif (210 bytes)arrow12Sure-cut Surfactant

Epoxy resins are easier to section with glass, as well as diamond knives, when the embedding medium contains 0.5-1.0% of our SURE-CUT. The life of the diamond knife will be extended with its use because it will reduce the friction of the plastic block during sectioning.

RT 21630   50g 28.00 Add to Cart

msdsAraldite 502Sym-Collidine, Buffer kit

Please see listing in Prepared Buffers.

RT 11500   one set 28.00 Add to Cart
RT 11510   box/5 sets 106.00 Add to Cart

msdsSym-Collidine, Reagent

Please see listing in Prepared Buffers.

RT 11520 Sym-Collidine 100 ml 150.00 Add to Cart

Certificates of Analysis


Tannic Acid to Z6040