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Electron Microscopy Sciences

Chemicals for Microscopy

Certificates of Analysis

arrow13Tannic Acid to Z6040

msdsarrowTannic Acid, Reagent, A.C.S., EM Grade

C76H52O46   F.W. 1701.28   CAS #1401-55-4

RT 21700   100 g 13.00 Add to Cart

msdsarrowTannic Acid, Reagent, A.C.S.

Gallotannin; Chiefly   (C14H10O9)n   F.W. range 1000.00-1500.00  CAS #1401-55-4
Low Molecular Weight. Suitable for electron microscopy

  • Takagi, M., Parmley, R.T., Denys, F.R. and Kageyama, M. (1983). Ultrastructural visualization of complex carbohydrates in epiphyseal cartilage with the tannic acid-metal salt methods. J. Histochem. Cytochem.,31:783
  • Simionescu, N. el al., J. Cell Biol., 70:608 (1976) A Combination of Tannic acid Glutaraldehyde Osmium Tetroxide (TAGO) Method. improving membranes contrast.
  • Roubos, E. W. and Van DerWal-Divendel, R.M. (1980). Ultrastructural analysis of peptide-hormone released by exocytosis. Cell Tiss. Res., 207:267
  • Buma, P.,Roubos, E.W. and Buijs, R.M. (1984). Ultrastructural demonstration of exocytosis of neural, neuroendocrine, and endocrine secretions with an in vitro tannic acid (TARI) method. Histochemistry, 80:247
  • Buma, P. and Nieuwenhuys, R. (1987). Ultrastructural demonstration of oxytocin and vascopressin release sites in the neural lobe and median eminence of the rat by tannic acid and immunogold methods. Neurosci. Lett., 74:151
  • Brooks, J.C. and Carmicheal, S.W. (1987). Ultrastructural demonstration of exocytosis in intact and saponin-permeabilized cultured bovine chromaffin cells. Am. J. Anat., 178:85
RT 21710   100g 62.00 Add to Cart

arrowTannic Acid Solutions

Please see listing in Reagents for Histology.

arrowTechnovit: Glycol Methacrylate Embedding Kits

Please see listing in Embedding Media Kits .

msdsarrowTMB, 3,3,5,5-Tetramethyl Benzidine

C16H20N2   F.W.240.35   CAS #54827-17-7
m.p. 166-169°C
Assay >98%
A non-carcinogenic substitute for DAB and DAB-HCl. Used as a sensitive and specific reagent for the detection of blood.

  • Standefer, J.C., Vanderjagt, D. (1977) Assay of Hemoglobin. Clin. Chem. 23, 749.
  • Boss, E.S., et al., (1981). Assay of Peroxidases. J. of Immunoassay. 2,187.
  • Holland, V.R., et al., (1974) Tetrahedron, 30, 3299.
0-5°C 13070   1g 27.00 Add to Cart

msdsarrowTMB-HCl, Dihydrochloride TMB

C16H20N2·2HCl   F.W. 313.30   CAS #64285-73-0
m.p. >300°C
Assay >98% (Cl)

0-5°C 13074 TMB-HCl 1gm 89.00 Add to Cart

Also available in tablet form. 1mg/ tablet, ready to use.

0-5°C 13075 TMB-HCl tablets 10/bottle 32.00 Add to Cart
0-5°C 13076 TMB-HCl tablets 50/bottle 140.00 Add to Cart

msdsarrow(TMA), Tetramethylene Glycol

(1,4-Butanediol dimethacrylate)
[H2C=C9CH3)CO2CH2CH2] 2   F.W. 226.68
CAS #2082-81-7
Specific Gravity (H2O=1) : 1.023
b.p. @4 mmHg 132-134°C
Inhibited with 200 ppm hydroquinone monomethyl ether.

RT 21750   100 ml 18.00 Add to Cart

msdsarrowTMS, Tetramethylsilane, 99.9%

Si(CH3)4   M.W. 88.22
b.p. 26.6-26.7°C
Flashpoint: 28°C

An organo-silicon compound tetramethylsilane (TMS) is used as an internal standard for nuclear magnetic resonance (NMR). It is chemically inert and has a boiling point of 26.6°C which is lower than acetone (53.6°C), and other highly volatile liquids, such as diethyl ether (34.5°C) and 1,2-epoxy propane (36.4°C). It is soluble in most organic solvents but insoluble in water. All of these properties make TMS an ideal solvent for tissue drying, which preserves excellent tissue surface details. Dey, Sudip. Et al. (1989). A new rapid method of air-drying for scanning electron microscopy using tetramethylsilane. J. of Microscopy, Vol 156, pp. 259-261.

RT 21760   100 ml 185.00 Add to Cart

msdsarrow(TC-NBT), Thiocarbamyl Nitro-Blue Tetrazolium

(2,2'-Di[p-nitrophenyl]-5,5'-di[p-thiocarbamylphenyl] -3,3'-[3,3'-dimethoxy-4,4'-biphenylene]ditetrazolium chloride)
C42H32Cl2N12O6S2   F.W. 935.8   CAS #36889-43-7

RT 21800   100mg 11.00 Add to Cart

arrowNitro-Blue Tetrazolium

Please see Nitro-Blue Tetrazolium.

msdsarrow(TCH), Thiocarbohydrazide, Highest Purity

H2NNHCSNHNH2   F.W. 106.15   CAS #2231-57-4
b.p. 171°C (decomposed)
Osmiophilic reagent.

An indefinite shelf life due to a special purification process.

  • Seligman A.M. et al (1965).Histochemical demonstration of some oxidized macromolecules with thiocarbohydrazide (TCH) or thiosemicarbazide (TSC) and osmium tetroxide. J. Histochem. Cytochem., 13:629
  • Seligman, A.M. et al (1966). A new staining method (OTO) for enhancing contrast of lipid-containing membranes and droplets in osmium tetroxide fixed tissue with osmiophilic thiocarbohydrazide (TCH). J. Cell Biol., 30:424
  • Thiery, J.P. (1967). Mise en evidence des polysaccharides sur coupes fines en microscopie electronique. J. Microsc., 6:987
  • Lo, H.K. et al (1987). A modified periodic acid-thiocarbo-hydrazide-silver proteinate staining sequence for enhanced contrast and resolution of glycogen depositions by transmission electron microscopy. J. Histochem. Cytochem., 35:393
  • Neiss, W.F. (1988). Enhancement of the periodic acid-Schiff (PAS) and periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) reaction in LR White sections. Histochemistry, 88:603
  • Hanker, J.S. et al (1964). Osmiophilic reagents: new cytochemical principles for light and electron microscopy. Science, N.Y., 146:1039
  • Derenzini, M., et al (1986). An improved periodic acid-thiosemicarbazide-osmium technique to reveal glyco-conjugates at the molecular level in situ. J. Histochem. Cytochem.,34:1161
RT 21900   5g 80.00 Add to Cart

msdsarrowThiosemicarbazide, Reagent, A.C.S.

NH2CSNHNH2   F.W. 91.14   CAS #79-19-6
m.p. 181-183°C
Assay >99.0%
References for use-see Thiocarbohydrazide

RT 22000   25g 12.00 Add to Cart

arrowTissue Marking Dyes

Please see listing in General Supplies for Histology.

msdsarrowToluene Reagent, A.C.S.

C6H5CH3   FW 92.14   CAS #108-88-3
Specific Gravity (H2O=1): 0.866
b.p. 110-1110C
Assay. 99.9%

Specifications:

Color (A.P.H.A.) 10
Residue after evaporation 0.001%
Subs. darkened by H2SO4 To pass test
Sulfur compound (as S) 0.003%
Water 0.03%
RT 22030   450 ml 10.50 Add to Cart
RT 22032   4x450 ml 38.00 Add to Cart

msdsarrowToluidine Blue 0, Certified, C.N. #DcU-10

(Basic blue; Methylene Blue T50 or T Extra)
C15H16N3SCl   FW 305.83   CAS #92-31-9
Dye Content: approx. 85% min.
Solubility: 3.25% Water; 1.75% Alcohol; 3.5% Cellosolve; 5.5% Glycol; 0.0% Xylene
*-A general stain for epoxy thick sections. Barajas et al.(1981) Identification of renal neuroeffector junctions by electron microscopy of reembedded light microscopic autoradiograms of semithin sections. J. Ultrastruct. Res. 77: 379.Campbell, R.D., And Hermans, C.O. (1972). A rapid method for resectioning 0.5 4.0 micron epoxy sections for electron microscopy. Stain Technol. 47:115.

RT 22050   50g 36.00 Add to Cart

Technical Tip

A Toluidine Blue/Basic Fuchsin Stain for GMA Embedded Tissue

msdsarrowTricresyl Phosphate

(Tritolyl Phosphate)
(CH3C6H4O)3PO   F.W. 368.37   CAS #1330-78-5
Specific Gravity (H2O=1): 1.16
A mixture of isomeric Tritolyl Phosphates, usually excluding the very toxic group ortho-isomer. Used to prepare benzoyl peroxide paste, which acts as a catalyst for water miscible resins.

RT 22150   225 ml 22.00 Add to Cart

msdsarrow2,4,6-Tri(Dimethylaminomethyl)-Phenol

Please see DMP-30.

RT 13600   25 ml 3.50 Add to Cart

msdsarrow2,4,6-Trinitrophenol

Please see Picric Acid .

RT 19550   100g 94.00 Add to Cart

msdsarrowTris (Hydroxymethyl) Aminomethane,Free-base, Reagent Grade

White crystalline powder
C4H11NO3   F.W. 121.14   CAS #77-86-1
A 40% (w/v) aqueous solution. It is clear and colorless.
Assay >99.9%
Water .<0.2%
Heavy Metals.-2ppm

RT 11700   100g 17.00 Add to Cart
RT 11720   500g 49.00 Add to Cart

msdsarrowTris (Hydroxymethyl) Aminomethane Maleate Buffer

Please see listing in Prepared Buffers

RT 11730   250 ml 21.00 Add to Cart
RT 11740   500 ml 28.00 Add to Cart

msdsarrowTris Buffer pH 10.0 (10x)

Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 10 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.
4-8°C 64142-11 Tris Buffer pH 10.0 100 ml 75.00 Add to Cart
4-8°C 64142-12 Tris Buffer pH 10.0 1000 ml 145.00 Add to Cart

msds arrow Triton® X-100

(Polyethylene glycol tert-octylphenyl ether)
CAS #9002-93-1
Specific Gravity (H2O=1): 1.065

RT 22140   100 ml 10.00 Add to Cart
RT 22142   500 ml 39.00 Add to Cart

msdsarrowTriton® X-100, 1% Aqueous Solution

Prepared 1% Triton X-100 in Deionized-Distilled water. Used for whole-cell preparations. Marek, L.F., and Kelly, R.O. (1983). A simple technique for the visualization of whole mount cytoskeletons with transmission electron microscopy. Anat. Rec. 207, 365. Pudney, J., and Singer, R.H. (1980). Intracellular filament bundles in whole mounts of chick and human myoblasts extracted with Triton X-100. Tissue cell 12, 595.

RT 22145   500 ml 41.00 Add to Cart

msdsarrowTriton® X-100, 0.08% Aqueous Solution

A prepared solution for the cleaning of diamond knives.

RT 22146   500 ml 39.00 Add to Cart

msdsarrowTrypsin 1:100, Powder

CAS #9002-07-7
Extract from Porcine Pancreas.
Activity >100 NF units/mg
Used for whole cell preparations. (see references in Triton X-100)

RT 22200   25g 22.00 Add to Cart

arrowmsdsmsdsTrypsin Reagent

Description: Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: This enzyme is supplied as concentrated form along with buffer. The kit comes in 2 sizes:
Small: Reagent B (Buffer) is 15 ml and the Reagent E (Enzyme) is 2 ml
Large: Reagent B (Buffer) is 100 ml and the Reagent E (Enzyme) is 10 ml
2-8°C 64142-01 Trypsin Kit Small kit 75.00 Add to Cart
2-8°C 64142-02 Trypsin Kit Large kit 145.00 Add to Cart

msdsarrow12-Tungstosilicic Acid

(Silicotungstic acid)
H4[SiO4.(W3O9)4]·xH2O   M.W. 2878.29+aq   CAS #11130-20-4

RT 22240   25g 62.00 Add to Cart

msdsarrowTyrode Solution

Ingredients: NaCl; KCl; CaCl2.6H2O; MgCl2.6H2O; NaHCO3; NaH2PO4; Glucose; and Distilled water.

Ordering Information:

RT 11760-05 Tyrode Solution 500 ml 24.00 Add to Cart
RT 11760-10 Tyrode Solution 1 L 33.00 Add to Cart

msdsarrowUnicryl Embedding and Staining Kit

Please see listing in Embedding Media Kits.

RT 14660   kit 300.00 Add to Cart

msdsarrowUranyl Acetate, Reagent, A.C.S.

UO2(OCOCH3)2·2H2O   F.W. 424.14
CAS #541-09-3
Assay 98.0-102.0%
Specifications:

Insoluble Matters 0.01
Chloride 0.003%
Sulfate 0.01%
Alkalies (as SO4) 0.05%
Heavy Metals 0.002%
Iron 0.001%

A universal EM stain for thin sections, en-block staining, and negative staining.

RT 22400   25g 155.00 Add to Cart

Image Galleryinfo available in pdf23405-01arrowUranyLess

"I am so happy. I read on your web site that UranyLESS hadn't been tested for negative staining and I wanted you to know that I think it did a spectacular job on this sample!" - Debra Townley, Baylor College of Medicine

Substitute for Uranyl Acetate

EMS is proud to introduce UranyLess, a new contrast stain solution for TEM, for all of your negative staining applications. It is an amazing substitute for Uranyl Acetate with similar results.

After only a minute of contact, UranyLess' fast-acting, non-radioactive lanthanide mix is finished staining your sections or deposits (see protocols below). If needed, lead citrate is recommended to increase the contrast. TIP: To prevent precipitates, use room temperature distilled water.

UranyLess's pH level is about 6,8 to 7. The 30ml airless bottle will stain approximately 1500 grids. The airless bottle increases the shelf life, eliminates CO2 contamination, and produces less waste - the solution pumps out in perfect amounts without leaking or spilling. UranyLess is also available in a larger amount for use in automated staining equipment. When using UranyLess for automated staining, do not wash longer than 10 minutes or you run the risk of losing all contrast.

23405-01UranyLess has been tested on many biological tissue (animal and plant): intestine, skeletal and cardiac muscle, liver, kidney, adrenal gland, nerve, cell culture, plant tissue, and also on negative staining of bacteriophage, bacteria, and polymers. UranyLess is ideal because of its ability to stain any kind of material and results are reproducible. To see the effectiveness of Uranyless, please visit the UranyLess Image Gallery.

arrowLead Citrate

UranyLess has a strong contrasting power, however, we recommend a Lead Citrate counterstain to enhance the contrast. You can follow the protocol of the UranyLess / Lead Citrate double contrast by watching the video. EMS recommends Lead Citrate, Ready to use, available in a 30 ml Airless bottle. The special pump delivers the product without letting in air, thus preserving the solution and preventing CO2 dissolution. It is convenient to use because it performs in any position, even upside down.

References:

"Easier and Safer Biological Staining: High Contrast UranyLess Staining of TEM Grids using mPrep/g Capsules." Benmeradi N1,2, Payre B2 and Goodman SL3.

  1. Delta Microscopies, 22, B route de saint Ybars, La côte blanche, 31190, Mauressac, France
  2. Université Toulouse, CMEAB Faculté Medecine, 118 route Narbonne, 31062, Toulouse, France
  3. Microscopy Innovations LLC, 213 Air Park Rd, Suite 101, Marshfield, WI, 54449, USA

"C-Nap1 mutation affects centriole cohesion and is associated with a Seckel-like syndrome in cattle." Nature Communications. Published 23 Apr 2015. Sandrine Floriot, all.

RT 22409 UranyLess EM Stain* msds 30 ml 50.00 Add to Cart
RT 22409-20 UranyLess EM Stain 200 ml 225.00 Add to Cart
RT 22410 Lead Citrate*, Ready to use msds 30 ml 40.00 Add to Cart
* in airless bottle

msdsarrowUAR-EMS Uranyl Acetate Replacement Stain

A non-radioactive substitute for uranyl acetate with comparable results. Avoids the tedious authorization, shipping and disposal issues involving uranyl acetate without sacrificing performance. Two lanthanide salts, samarium and gadolinium triacetate, effectively used for staining plastic-embedded animal and plant tissues. Also for negative staining of macromolecules. Not recommended as a fixative.

RT 22405 UAR-EMS Stain 25 ml 70.00 Add to Cart

msdsarrowPlatinum Blue, an EM Stain

IBI Blue Platinum Stain Pt4N8H6O24C20

An EM stain that may have the ability to replace Uranyl Acetate in some instances. If you have issues with having Uranium salts that are radioactive (depleted or not) in the lab this may be the answer for you. An alternative for staining TEM thin sections

RT 22407 Platinum Blue 1 ml 225.00 Add to Cart

arrowUranyl Acetate Solution

We are now offering Uranyl Acetate pre-made and ready to use solution for making your work easier. The following percentages are avaialble off the shelf (1, 2, 3, 4). Other percentages are avaialble upon request.

RT 22400-1 1% Uranyl Acetate Solution msds 100 ml 36.00 Add to Cart
RT 22400-2 2% Uranyl Acetate Solution msds 100 ml 43.00 Add to Cart
RT 22400-3 3% Uranyl Acetate Solution msds 100 ml 62.00 Add to Cart
RT 22400-4 4% Uranyl Acetate Solution msds 100 ml 74.00 Add to Cart

msdsarrowUranyl Formate

UO2(HCO2)2·H2O

F.W. 378.08
CAS #16984-59-1
For ultra fine grain structure, our Uranyl Formate offers amazing advancement in negative staining for microscopy.

Specifications:

Purity 99.9-100%
Specific Gravity 3.695
Appearance Yellow Crystals
Melting Point 110°C

Ordering Information:

RT 22450 Uranyl Formate 1 gram 102.00 Add to Cart
RT 22451 Uranyl Formate 5 grams 490.00 Add to Cart

msdsarrowUranyl Magnesium Acetate

UO2Mg(CH3COO)4

F.W.530.0

CAS # 20596-93-4

Used as a substitute for uranyl acetate in either double-staining techniques and also as a solo stain.

RT 22500 Uranyl Magnesium Acetate 5 grams 190.00 Add to Cart
RT 22501 Uranyl Magnesium Acetate 1 gram 40.00 Add to Cart

msdsarrowUranyl Nitrate, Hexahydrate

Reagent Grade, A.C.S.
UO2(NO3)2·6H2O

F.W. 502.13

CAS #13520-83-7

Specifications:

Purity 99.9-100%
Density 2.807 g/cm3
Solubility ~66g/100g H2O
Melting Point 60°C
Boiling Point 118°C
Insoluble Matter <0.005%
Chloride (Cl) <0.002%
Sulfate (SO4) <0.005%
Alkalies % Alkaline Earths (Sulfates) <0.1%
Heavy Metals (as Pb) <0.002%
Iron (Fe) <0.002%
Substances Reducing Permanganate (as UIV) <0.06%

A Universal EM Negative Stain used for viruses. In tissue samples it stabilizes nucleic acids and cell membranes. These solutions are more stable than uranyl acetate and they react primarily with negatively charged groups in the absence of phosphate ions.

RT 22600 Uranyl Nitrate 5 grams 120.00 Add to Cart

msdsarrowUranyl Oxalate

(COO)2 UO2 ·.3H2O

M.W.412.2

To adjust the pH of Uranyl compounds as well as a negative stain for electron microscopy.

RT 22510 Uranyl Oxalate 1 gram 225.00 Add to Cart

msdsarrowUranyl Zinc Acetate

ZnUO2(CH3COO)4

CAS # 10138-94-0

F.W. 571.59

Used as a laboratory reagent in the determination of sodium concentrations of solutions.

RT 22610 Uranyl Zinc Acetate 5 grams 190.00 Add to Cart

Technical Tips:

Avoiding Contamination of Ultra-Thin Sections
Removal of Lead Citrate and Uranyl Acetate Precipitates on LR White Sections

msdsarrowVan Gieson's Solution

To use with Weigert's Iodine or Weigert's Iron as a connective tissue stain.

RT 26046-05 Van Gieson's Solution 500 ml 32.00 Add to Cart
RT 26046-06 Van Gieson's Solution 1 L 54.00 Add to Cart

msdsarrowWater Deionized, Reagent Grade A.C.S.

H2O F.W. 18.02 CAS #7732-18-5
EMS Reagent Grade Water is typically prepared at 18 megohm/cm specific resistance using a reverse osmosis, mixed deionization, activated filtration and final filtration at 0.2 microns.
Color (APHA) <+/-5
Bacteriological purity 0 CFUs/L
Residue after evaporation 10ppm
Coliform negative

RT 22800-01 Deionized Water 1 gal 10.00 Add to Cart
RT 22800-05 Deionized Water 5 gal 42.00 Add to Cart

msdsarrowWeigert's Iodine Solution

A histological stain. Demonstration of connective tissue is useful in cases of emphysema, arteriosclerosis and other vascular diseases

RT 26045-05 Weigert's Iodine Solution 500 ml 32.00 Add to Cart
RT 26045-06 Weigert's Iodine Solution 1 L 57.00 Add to Cart

msdsmsdsarrowWeigert's Iron Hematoxylin

Stain selectivity for nuclei, fungal, and general applications. Ready-to-use.

RT 26044-05 Weigert's Iron Hematoxylin A 500 ml 38.00 Add to Cart
RT 26044-06 Weigert's Iron Hematoxylin A 1 L 71.00 Add to Cart
RT 26044-15 Weigert's Iron Hematoxylin B 500 ml 28.00 Add to Cart
RT 26044-16 Weigert's Iron Hematoxylin B 1 L 48.00 Add to Cart

msdsarrowWright Stain, Certified #DcWr-38

CAS #68988-92-1
Solubility: 0.091% Water; 0.23% Alcohol

RT 22720   25 g 28.00 Add to Cart

msdstechnical data sheetarrowWright Staining Solution

For use as a blood stain. Ready-to-use.

RT 26060-05 Wright Stain Solution 500 ml 32.00 Add to Cart
RT 26060-06 Wright Stain Solution 1 L 59.00 Add to Cart

msdsarrowXylenes, Reagent, A.C.S.

(Xylol, Dimethylbenzene)
C6H4(CH3)2   FW 106.27   CAS #1330-20-7
Specific Gravity (H2O=1): 0.865
b.p. 136-140°C
This reagent is a mixture of isomers (ortho, meta, and para) and may contain Ethylbenzene.

Specifications:

Color (A.P.H.A.) 10
Subs. darkened by H2SO4 To pass test
Sulfur Compounds (as S) 0.003%
Water 0.05%
Residue after evaporation 0.002%
RT 23400   450 ml 8.00 Add to Cart

msdsarrow12Xylene: Peanut oil, 2:1

RT 23402 Xylene:Peanut oil, 2:1 500 ml 43.00 Add to Cart

msdsarrow12Xylene Substitute

A xylene substitute for use in histology and cytology. It is safer and a more preferable alternative to xylene. It can be used as a solvent and clearing agent, as well as for dissolving paraffin waxes, glues and adhesives.

Properties:

  • Minimal tissue shrinkage.
  • Dries slowly with no residue.
  • Less toxic than xylene.
  • Soluble with alcohol, paraffin embedding media, and mounting media.

It can be used in all procedures that require xylene. Biodegradable.

RT 23410-01 Xylene Substitute 1 gal 102.00 Add to Cart
RT 23410-04 Xylene Substitute 4x1 gal 375.00 Add to Cart
RT 23410-05 Xylene Substitute 5 gal 390.00 Add to Cart

msdsAlcoholic Stain Solutionsarrow12Xylene Substitute – Hemo-De®*

An intensive study from 1992 throughout 2000 has shown that Hemo-De® (d-Limonene) a terpene-based chemical with a pleasant citrus fragrance, was able to replace many of the clinical laboratories toxic reagents, such as carbol-xylene, xylene, ethyl acetate and formalin.

In Parasitology – Hemo-De replaces ethyl acetate in the concentration procedure; Hemo-De replaces carbol-xylene, xylene and formalin in the trichrome procedure.

In Histology – Hemo-De replaces xylene in most all procedures. Hem-De is a superior solvent and Clearing agent that can be used in all tissue processing, deparaffiniating and slide preparation.

Hemo-De Features:

  • Soluble with alcohol and mounting media
  • Biodegradable, non-corrosive, non-flammable (Combustible)
  • Contains no benzene and no toluene
  • Low toxicity levels
  • Minimal tissue shrinkage
  • Reasonably fast drying and leaves no residue

Hemo-De Application:

In Research and Medical – Hematology, Cytology, Pathology, Microbiology, Histology, Pathology, Hospital

In Mechanical Applications – Cleaning of machine parts, cleaning tools, engines etc.

In Electronic Applications – Circuits boards, electronic components, tools.

General Applications – Clean metal and glass surfaces. Dilute with water can be used to clean all plastic surfaces. Removal of adhesives, glues, tars…

Hem-De Information:

Chemical name: d-Limonene (Terpene Hydrocarbon, n.o.s.)
Composition: d-Limonene, CAS [5989-27-5]98.0%
Butylated Hydroxyanisolve (BHA), CAS
[25013-16-5]…….<0.024%
Flash Point (TCC): 121°F (49.4°C)
Boiling Point: 349°F (176°C) @ 760mmHg
Freezing Point: -74°C
Shipping: Terpene Hydrocarbons, n.o.s. ID No.
04858003, Flammable Liquid, Group III

References:

  1. W.E. Aldeen and D. Hale. "Use of Hem-De to eliminate toxic agents used for concentration and trichrome staining of intestinal parasites" J. of Clinical Microbiology, July 1992, 1893- 1895, Vol. 30, No. 7
  2. Metzger, Zvi DMD et al. "Gutta-Percha Softening: 'Hem-De' as a Xylene Substitute" J. of Endodontics". 26(7):385-388, July 2000.
  3. Devon C. Hale, MD; et al. "Use of a Single Slide Trichrome- Stained Concentrate for the detection of intestinal Parasites Stained Concentration Procedure for Ova and Parasites" American Journal of Clinical Pathology, Vol. 106, N0. 2. Aug. 1996.
  4. J. M. Miller et al. "Biodegradable, effective substitute for xylene in the Ehrlich indole procedure". J. of Clinical Microbiology, Aug. 1994, 2028-2030, Vol 32, No 8.
RT 23412-01 Xylene Substitute 1 gal 110.00 Add to Cart
RT 23412-04 Xylene Substitute 4x1 gal 399.00 Add to Cart
RT 23412-05 Xylene Substitute 5 gal 450.00 Add to Cart

*Hemo-De® is a product of Scientific Solvents.

msdsmsdsarrow12Histo-Clear®/Histo-Clear II®

Histo-Clear® (Distilled essential oils –food grade) and Histo-Clear II® (A mixture of Aliphatic Hydrocarbon and Distilled essential oils – food grade, reduced citrus odor) are excellent non-toxic clearing agents replacing Xylene.

RT 64110-01 Histo-Clear® 1 L 30.00 Add to Cart
RT 64110-04 Histo-Clear® 1 gal 80.00 Add to Cart
RT 64110-10 Histo-Clear® 5 gal 395.00 Add to Cart
RT 64111-01 Histo-Clear II® 1 L 19.00 Add to Cart
RT 64111-04 Histo-Clear II® 1 gal 64.00 Add to Cart
RT 64111-10 Histo-Clear II® 5 gal 295.00 Add to Cart

msds23405-01arrow12Histosol™ - Xylene Substitute

Histosol™ is the original high flash point (114°F TCC) histological clearing agent. It is intended to be used as a replacement for xylene where the hazards associated with aromatic hydrocarbon vapors are to be reduced. Museum-quality tissue slides can be prepared with Hitosol without change in protocol or procedure. Histosol is manufactured from petrochemical products and is miscible in all proportions with ethanol, isopropanol, and t-butanol. It is also miscible with all paraffin-based tissue embedding media and all permanent mounting materials.

RT 23405-01 Histosol – Xylene Substitute 1 gal 59.00 Add to Cart
RT 23405-04 Histosol – Xylene Substitute 4 x 1 gal 216.00 Add to Cart

msdsarrowZ6040 Embedding Primer

This unique primer affords you a better adhesion between your resin and specimen that you are embedding and prevents the sample from pulling away from the resin during sectioning.

RT 50440-10 Z6040 Primer 100 ml 44.00 Add to Cart

Certificates of Analysis


Embedding Media Kits: Araldite to Styrene arrow13arrow13