Cryo Supplies and Accessories
EMS UVC3 Ultraviolet Cryo Chamber
The EMS UVC3 Cryo Chamber has been specifically designed to provide an economical yet precisely controlled low temperature environment for polymerization of polymers and resins for cryo specimen preparation. It can also be used for dehydration and infiltration of cryo specimens. This simple and functional unit has a large insulated chamber and comprises fully automatic temperature control for the polymerization process of embedding resins and polymers.
Features
- Liquid crystal display and PLC control
- Fan cooled electronic ballast for UV lamp tubes
- Temperature range can be set from -10°C to -40°C (14°F to -40°F)
- Includes memory function for Min/Max temperatures during any run
- 365nm ultraviolet lamps thermally shielded by dual pane Plexiglas®
- Holds up to 66 samples with BEEM® Cryo Capsule Holders
- Perfect for Unicryl, LR White, LR Gold, Lowicryls, JB-4, Quetols and all other resins cured by 365nm UV radiation
- Universal voltage
Operation
- Add dry ice to the chamber
- Set temperature (-10°C to -40°C / 14°F to -40°F)
- Turn on UV lamp and cure embedding resins
Specifications
| UV Light Source | Two 15-watt self-filtering long wavelength tubes 1600 µW/cm_ of 365nm UV radiation at 15cm (6") |
| Temperature Control | -10°C to -40°C (14°F to -40°F) Typically +/- 1.5 °C |
| Control Type | LCD Display with PLC with function buttons |
| Exterior Dimensions | 64.l L x 36.8 W x 48.9cm H (25.25" L x 14.50" W x 19.25" H) |
| Interior Dimensions | 56.5 L x 30.5 W x 33.7cm H (22.25" L x 12" W x 13.4" H) |
| Power Requirements | 110 - 240VAC, 50/60Hz (240V-0.5A, 110V-1A) |
| Weight | 11.4 kg (25 lbs.) / Shipping weight: 18.2kg (40 lbs.) |
| 70445-10 | EMS UVC3 Cryo Chamber, 120 VAC | each | 1,835.00 | Add to Cart |
| 70445-20 | EMS UVC3 Cryo Chamber, 220 VAC | each | 1,935.00 | Add to Cart |
| 70022-04 | EMS Embedding Capsule Holders | 4/pack | 36.00 | Add to Cart |
| 70120-04 | Gelatin "00" Capsule Holder | 4/pack | 60.00 | Add to Cart |
Sample Pin for Cryo Ultramicrotomes
This cryo pin has a diameter of 2 mm and is compatible with the Leica and RMC cryo ultramicrotomes.
Features
- Made from cryo compatible aluminium
- Concentric grooves on the sample surface ensure better adhesion
- Reversed tapered head with a diameter of 3.5 mm
- 10 mm long
| 75959-05 | Sample pin for cryo ultramicrotomes, 2 x 10 mm, aluminium | 10/pk | 16.00 | Add to Cart |
| 75959-06 | Sample pin for cryo ultramicrotomes, 2 x 10 mm, aluminium | 50/pk | 76.00 | Add to Cart |
Specimen Pins; Ultra Microtomy
Aluminum specimen mount pin for mounting samples for ultramicrotomy. Available in two types: Slotted Screw and Bullseye.
| 70446 | Aluminum Specimen Pin, Bullseye | 50/pk | 113.00 | Add to Cart |
| 70447 | Aluminum Specimen Pin, Slotted Screw | 50/pk | 113.00 | Add to Cart |
EMS Golden Brushes; Nylon
These brushes are perfect for cryomicrotomy – for controlled manipulation of frozen sections in the cryostat, as well as cryomicrotome. Easier to clean than animal hair brushes and more durable, they come in 4 sizes: ¼", ½", ¾", and 1" for large sections. These brushes are available individually or in a set of all 4 sizes.
| 66110-01 | EMS Golden Brush ¼" | each | 4.25 | Add to Cart |
| 66110-02 | EMS Golden Brush ½" | each | 4.25 | Add to Cart |
| 66110-03 | EMS Golden Brush ¾" | each | 4.25 | Add to Cart |
| 66110-04 | EMS Golden Brush 1" | each | 4.25 | Add to Cart |
| 66110-05 | EMS Golden Brushes, set of 4 | 4/pk | 10.00 | Add to Cart |
Cryogenic-Gel
- Replaces the messy "runny" embedding media.
- Easy to use.
- Sticks to where you want it.
- Will not run – No waste.
It is a highly viscous, water-soluble embedding medium for frozen sections that "stays put". The gel is packaged in a pump-dispenser bottle that can instantly dispense the gel in any position. The dispenser has a built-in "guillotine" that cleanly severs the gel from the pump spouts and prevents any "stringy" medium from pulling away when the bottle is removed. To use, simply dispense gel on a room temperature block-holder. Use a cold heat extractor to form a base layer. Dispense additional gel. Place the tissue on top of the gel. Use a cold heat extractor to freeze tissue and form a block.
| 62806-01 | Cryo-Gel™, 4.3oz | each | 21.00 | Add to Cart |
| 62806-06 | Cryo-Gel™, 4.3oz | 12/cs | 210.00 | Add to Cart |
Tissue-Tek® O.C.T. Compound
Sakura Finetek - This is a well known water soluble glycol’s and resins compound that provides an excellent specimen matrix for cryostat sectioning at temperatures of -10°C and below. It leaves no residue during the staining procedure.
| Not for sale overseas | |||||
| Catalog # | Tissue-Tek | Description | Pack | Price | |
| 62550-01 | 4583 | O.C.T. Compound | 4 oz | 15.95 | Add to Cart |
| 62550-12 | 4583 | O.C.T. Compound | 12/cs | 168.00 | Add to Cart |
PolarStat Plus™ Embedding Medium for Frozen Sections
This formulation is especially well suited for Mohs cryostat sectioning. It freezes at optimum cutting temperatures of -10°C and below. No residue is left on slides during the staining procedure, thereby eliminating undesirable background staining.
| 19221-01 | PolarStat Plus™ Embedding Medium for Frozen Sections | each | 13.50 | Add to Cart |
| 19221 | PolarStat Plus™ Embedding Medium for Frozen Sections | 12/pk | 130.00 | Add to Cart |
Polarstat™ Embedding Medium
An aqueous-based frozen section embedding medium designed to support tissue blocks in cryostat sectioning. Formulated to promote rapid freezing, enhanced sectioning and consistent results at a working temperature of -20°C.
4 oz.
| 19222 | Polarstat™ Embedding Medium | each | 12.00 | Add to Cart |
CytoFix
Cytology fixative spray designed for slide based cytology specimens for cellular preservation and transport.
Quickly spray a fine mist over the slide (covering the entire specimen) for cellular fixation and reduce air drying of the material.
CytoFix forms a thin, tough, clear protective coating over the specimen with a water and alcohol solution of Polyethylene glycol for cellular fixation and does not require rehydration by the laboratory prior to staining. Allows safe storing or transporting of slides.
Allow specimens to dry before sending to lab or for storage.
- Dries in 4 to 6 minutes
- Water soluble
- Won't cloud or obscure specimen
- Contains no chlorofluorocarbons
Available in pump bottle or aerosol can.
| 22805-01 | CytoFix (Non-Aerosol) | 100 ml | 14.50 | Add to Cart |
| 22805-02 | CytoFix Can | 4 oz | 14.50 | Add to Cart |
Tissue Freezing Medium; TFM™
An embedding matrix for frozen sections. TFM™ is an ultra pure formulation of water-soluble glycols and resins that provides a solid bond between the tissue and the object holder with the following features:
- Less Ice Artifacts: TFM's reduced water content minimizes freeze-fracturing
- Less Curling: TFM allows you to pick up flat serial sections with ease
- Freezes Faster: TFM freezes very fast and offers better turn-around time
- Completely water soluble: TFM reduces tissue dislodging
- Now available in 5 colors: clear, yellow green, red and blue
| RT | 72592 | Tissue Freezing Medium, clear | |
4 oz | 30.00 | Add to Cart |
| RT | 72593 | Tissue Freezing Medium, clear | 12x4oz | 333.00 | Add to Cart | |
| RT | 72592-Y | Tissue Freezing Medium, Yellow | |
4 oz | 30.00 | Add to Cart |
| RT | 72593-Y | Tissue Freezing Medium, Yellow | 4x4oz | 120.00 | Add to Cart | |
| RT | 72592-G | Tissue Freezing Medium, Green | |
4 oz | 30.00 | Add to Cart |
| RT | 72593-G | Tissue Freezing Medium, Green | 4x4oz | 120.00 | Add to Cart | |
| RT | 72592-R | Tissue Freezing Medium, Red | |
4 oz | 30.00 | Add to Cart |
| RT | 72593-R | Tissue Freezing Medium, Red | 4x4oz | 120.00 | Add to Cart | |
| RT | 72592-B | Tissue Freezing Medium, Blue | |
4 oz | 30.00 | Add to Cart |
| RT | 72593-B | Tissue Freezing Medium, Blue | 4x4oz | 120.00 | Add to Cart |
LV CryoOil™
Oils are widely used in crystallography – as cryoprotectants when samples are flash cooled, and as barriers to the dehydration, solvent evaporation, and oxidation, that may occur in air near and above room temperature. The ice ring seen in the diffraction pattern of flash cooled protein crystals arise primarily from crystallization of the aqueous solution surrounding the crystal, not of the internal solvent. Oils can displace and replace this surrounding solution with little risk of damage due to osmotic shock.
Substantial dehydration-induced damage can easily occur between removed from a drop and flash cooling. Dehydration can be minimized by first transferring to an oil drop (using MicroSieve™, in MicroTool kit 1 and MicroTool Kit 2) before mounting the oil coated crystal on a MicroMount or Micromesh.
However, surrounding oil scatters X-rays, reducing diffraction signal-to-noise, and contributes thermal mass that reduces crystal cooling rates and increases the need for penetrating cryoprotectants. These problems are especially acute for the smallest crystals. When mineral oil, Paratone Oil and other high viscosity oils are used, the volume of surrounding oil can exceed the crystal volume by a factor of 10 or more., and can contribute excess drag that increases sample motion in a cryostream. For larger rod or plate shaped crystals, the surrounding oil exerts stresses when it freezes that can cause crystal cracking and mosaic broadening.
LV CryoOil™ Is the best solution to your oil needs. It has the lowest viscosity of any available perfluoropolyether oil (1/10 that of Paratone oil and compatible to that of vegetable oil) and very low surface tension (less than 1/3 that of water). Consequently, a dip in this oil followed by gentle tapping to shake off excess can yield protective oil films on your crystals of as little as 10 µm thick.
LV CryoOil™ has extremely low vapor pressure, excellent chemical inert and excellent thermal stability.
Ordering:
LV CryoOil™ comes in each vial 1.5 ml, enough to protect hundreds of crystals.
| 60166 | LV CryoOil™ | 1.5 ml vial | 62.00 | Add to Cart |
Quick Freeze; Shur/Freeze, Environmentally Safe
A high purity 1, 1, 1, 2 - Tetrafluoroethane (R134a) that you can rely on to solidify paraffin sections of fresh tissue without disrupting cellular structure. A short spray burst will instantly freeze small area to -45°C/-5°F. Environmentally Safe and non-flammable.
| 72595 | Quick Freeze; Shur/Freeze | 10 oz | 15.25 | Add to Cart |
| 72596 | Quick Freeze; Shur/Freeze | 12 x 10 oz | 175.00 | Add to Cart |
CytoCool II
For the Flash freezing of tissue specimens in the Cryostat as well as being used to harden difficult to cut tissue on a microtome during the sectioning. CytoCool II is an aerosol that does not contain any ozone depleting (CFC'S). Controlling the tissue freezing is simply done by varying the finger pressure on the trigger nozzle. This unique feature helps to prevent over cooling of the specimen.
| 72597 | CytoCool II Aerosol | 11 oz | 32.00 | Add to Cart |
| 72597-12 | CytoCool II Aerosol | 12 x 11 oz | 340.00 | Add to Cart |
Frostbite® Rapid Coolant
Ozone-safe, but just as effective.
Frostbite Rapid Coolant contains no CFCs yet maintains the ability to rapidly cool paraffin blocks and tissue. Two quick bursts will ensure deep penetration into both paraffin blocks and fresh tissue specimens.
- The trigger nozzle and needle tube help to localize spray, reducing excess coverage and eliminating waste
- Nonflammable
| 72594-01 | Frostbite® Rapid Coolant 10 oz. (300 ml) Can | each | 18.00 | Add to Cart |
| 72594-10 | Frostbite® Rapid Coolant 10 oz. (300 ml) Can | 6/cs | 105.00 | Add to Cart |
| 72594-20 | Frostbite® Rapid Coolant 10 oz. (300 ml) Can | 12/cs | 198.00 | Add to Cart |
Mr. Frosty® Cryo 1°C Freezing Containers
Polycarbonate container, high-density polyethylene closure, tube holder, foam insert
Mr. Frosty Cryo 1°C Freezing Containers are inexpensive, reusable and convenient devices for freezing biological samples. Freeze cells in tubes from 1 to 5 ml using the Mr. Frosty™ Freezing Container. The simple-to-use system is designed to achieve a rate of cooling very close to -1°C/minute, the optimal rate for cell preservation. Isopropyl alcohol is necessary to achieve the recommended rate of cooling.
Features:
- Holds 12 to 18 tubes and has profile height of 86 or 151 mm (3-7/16 to 5-5/16 in.), which saves precious freezer space during the cooling process
- Screw top lid secures samples inside in the event of accidental container tipping
- Eliminates unreliable, improvised foam container devices
- Easy to use – requires only 100% isopropyl alcohol and a mechanical freezer
- No contact with the alcohol: no wicking, no label removal and no compromise of tube hand printing
- Containers have 4.75 in. diameter (120 mm) closure
- Available in 3 convenient tube capacities
Catalog # |
Description |
Tube Capacity |
Pack |
Price |
|
| 72596-01 | Mr. Frosty® Cryo 1°C Freezing Container | 1.0-2.0 ml | each | 99.00 | Add to Cart |
| 72596-02 | Mr. Frosty® Cryo 1°C Freezing Container | 3.6 ml | each | 99.00 | Add to Cart |
| 72596-03 | Mr. Frosty® Cryo 1°C Freezing Container | 4.5-5.0 ml | each | 99.00 | Add to Cart |
Wrist
Mid-Arm
Elbow
Shoulder
Waterproof Cryo-Grip™ Gloves
Hand and Arm Protection When Working with Liquid Nitrogen
Made from state-of-the-art materials, these waterproof cryogenic gloves are designed to keep you dry and to wick moisture away from your hands, so you can work in comfort for extended periods of time.
The multi-layered construction allows for a maximum level of grip, dexterity, flexibility, and thermal protection – essential features when function is important and safety is critical.
They offer improved grip on small objects while protecting your hands and arms when working in hazardous, ultra-cold environments.
They are designed to provide a high level of thermal protection where the exposure to cryogenic fluids might exist.
Available in four different lengths: wrist, mid-arm, elbow, and shoulder. Each length available in sizes S, M, L, and XL.
Features
- Improved grip
- Excellent dexterity
- Maximum thermal protection
- Lightweight
- Comfortable
- Waterproof
- Breathable
- Stay-dry lining
| 71126-x | Cryo-Grip Gloves, Wrist | 1 pair | 169.00 | |
| 71127-x | Cryo-Grip Gloves, Mid-Arm | 1 pair | 180.00 | |
| 71128-x | Cryo-Grip Gloves, Elbow | 1 pair | 215.00 | |
| 71129-x | Cryo-Grip Gloves, Shoulder | 1 pair | 267.00 |
Cryo-Gloves®
We now offer cryogenic personal protective garments of the highest quality. Cryo-Gloves® and Cryo-Aprons® are essential accessories for those working in ultra cold environments. Providing effective protection in a wide variety of applications.
Features
- Protection from –135°C to 150°C
- Superior thermal protection
- Water-resistant
- Comfortable / Lightweight / Excellent dexterity
- Breathable / Stay-dry lining.
Applications
- Ultra low and Blast freezers
- Cold rooms
- Dry ice handling
- Liquid Nitrogen, liquid oxygen, liquid helium
Environment
- Clinical Laboratory
- Bio-Medical
- Blood Banks
- Frozen food processing
- Pharmaceutical
| 71070 | Wrist Cryo-Gloves®, Small | 12" long | 1 pair | 148.00 | Add to Cart |
| 71071 | Wrist Cryo-Gloves®, Medium | 12" long | 1 pair | 148.00 | Add to Cart |
| 71072 | Wrist Cryo-Gloves®, Large | 12" long | 1 pair | 148.00 | Add to Cart |
| 71072-X | Wrist Cryo-Gloves®, X-Large | 12" long | 1 pair | 148.00 | Add to Cart |
| 71073 | Mid Arm Cryo-Gloves®, Small | 14" to 15" long | 1 pair | 150.00 | Add to Cart |
| 71074 | Mid Arm Cryo-Gloves®, Medium | 14" to 15" long | 1 pair | 150.00 | Add to Cart |
| 71075 | Mid Arm Cryo-Gloves®, Large | 14" to 15" long | 1 pair | 150.00 | Add to Cart |
| 71075-X | Mid Arm Cryo-Gloves®, X-Large | 14" to 15" long | 1 pair | 150.00 | Add to Cart |
| 71076 | Elbow Cryo-Gloves®, Small | 18" to 20" long | 1 pair | 185.00 | Add to Cart |
| 71076-M | Elbow Cryo-Gloves®, Medium | 18" to 20" long | 1 pair | 185.00 | Add to Cart |
| 71077 | Elbow Cryo-Gloves®, Large | 18" to 20" long | 1 pair | 185.00 | Add to Cart |
| 71078 | Elbow Cryo-Gloves®, X-Large | 18" to 20" long | 1 pair | 185.00 | Add to Cart |
| 71079-S | Shoulder Cryo-Gloves®, Small | 27" to 28" long | 1 pair | 237.00 | Add to Cart |
| 71079-M | Shoulder Cryo-Gloves®, Medium | 27" to 28" long | 1 pair | 237.00 | Add to Cart |
| 71079-L | Shoulder Cryo-Gloves®, Large | 27" to 28" long | 1 pair | 237.00 | Add to Cart |
| 71079-XL | Shoulder Cryo-Gloves®, X-Large | 27" to 28" long | 1 pair | 237.00 | Add to Cart |
Cryo-Gloves® and Cryo-Aprons® are registered trademarks of Tempshield, Mt Desert ME.
Cryo-Apron®
These aprons are 100% water-proof with adjustable straps, for neck and waist.
| 71080 | Cryo-Apron®, Small 36" | each | 150.00 | Add to Cart |
| 71081 | Cryo-Apron®, Medium 42" | each | 160.00 | Add to Cart |
| 71082 | Cryo-Apron®, Large 48" | each | 175.00 | Add to Cart |
| 71082-X | Cryo-Apron®, X-Large 54" | each | 199.00 | Add to Cart |
Cryo-Protection® Face Shield
The Cryo-Protection Face Shield is not only meant to be used in cryo environments, but in any laboratory environment where face protection is needed. This shield is made from molded Polycarbonate and protects your entire face as well as your upper chest. The head gear offers a two-way adjustable fit.
Features
- Impact-resistant
- Lightweight. Meets ANSI 787.1
- Optically clear
- Shield and head gear resist cryo temperatures
| 71079-10 | Cryo-Protection® Face Shield | each | 70.00 | Add to Cart |
Safety Goggles
Two styles: vented or perforated
- The vented safety goggles help avoid chemical contact.
- Scratch resistant lenses
| 64475-01 | Safety Goggle, 4 Plastic Vent | each | 4.00 | Add to Cart |
| 64475-10 | Safety Goggle, 4 plastic Vent | 10/pk | 40.00 | Add to Cart |
| 64476-01 | Safety Goggle, Perforated Frame | each | 4.00 | Add to Cart |
| 64476-10 | Safety Goggle, Perforated Frame | 10/pk | 35.00 | Add to Cart |
| 64477-01 | Safety Goggle, Perforated Frame, Small Size (5¾" x 2½") | each | 4.00 | Add to Cart |
| 64477-10 | Safety Goggle, Perforated Frame, Small Size (5¾" x 2½") | 10/pk | 35.00 | Add to Cart |
EMS Cryo Preparation Kit
Electron Microscopy Sciences is proud to introduce a cryo starter kit which includes all of the supplies necessary to begin Cryo EM. The kit includes a variety of our Cryogenic Grid Storage Boxes, Manipulation Tweezers and Gripper Tools, as well as our C-Flat Holey Carbon Cryo Grids. The Tweezers we are using are coated as well as anti-magnetic.
The kit comes with the following pieces in a small display box:
- Cryo Grid Box, Round, w/Lid
- Cryo Grid Box, Square, w/Lid
- Cryo Grid Box, Round, wo/Lid
- Cryo Grid Box, w/Pin Type Lid for FEI Vitrobot
- Cryo Grid Box, w/Pin Type Lid
- Cryo Grid Box, w/Lid, Sq for Gatan CT3500
- Gripper Tool for Cryo Grid Boxes
- C-flat™ Holey Carbon Grids for TEM - Copper Only (CF-222C-25)
- ESD Ergonomic Tweezers (0304-5SAESD-ET2)
- ESD Epoxy-Coated Tweezers (0302-5-CO)
| 71166-K | Cryo Preparation Kit | kit | 375.00 | Add to Cart |
Additional Kit Supplies
| 71166-10 | Cryo Grid Box, Round, w/Lid | each | 9.95 | Add to Cart |
| 71166-10-G | Cryo Grid Box, Round, w/Lid, Green | each | 9.95 | Add to Cart |
| 71166-10-W | Cryo Grid Box, Round, w/Lid, White | each | 9.95 | Add to Cart |
| 71166-20 | Cryo Grid Box, Square, w/Lid | each | 9.95 | Add to Cart |
| 71166-30 | Cryo Grid Box, Round, wo/Lid | each | 7.50 | Add to Cart |
| 71166-40 | Cryo Grid Box, w/Pin Type Lid for FEI Vitrobot | each | 19.00 | Add to Cart |
| 71166-50 | Pin Type Lid only for Cryo Grid Box | each | 13.00 | Add to Cart |
| 71166-50B | Cryo Grid Box, w/Pin Type Lid | each | 19.00 | Add to Cart |
| 71166-60 | Cryo Grid Box, w/Lid, Sq for Gatan CT3500 | each | 18.50 | Add to Cart |
| 71166-SP | Gripper Tool for Cryo Grid Boxes | each | 36.00 | Add to Cart |
| Cat. # | Hole Size |
Hole Spacing |
TEM mesh |
TEM Grid |
Pack | Price | |
| CF-222C-25 | 2.0 µm | 2.0 µm | 200 | Cu | 25/pk | 239.00 | Add to Cart |
| Catalog | A mm | B mm | Finish | Handle | Alloy | Price | Unit | |
|---|---|---|---|---|---|---|---|---|
| 0304-5SAESD-ET2 | 0.06 | 0.10 | Polished | Black | Antimagnetic | 34.50 | each | Add to Cart |
| 0302-5-CO | 0.06 | 0.1 | Electronic | Epoxy Coated |
Inox 02 | 28.00 | each | Add to Cart |
Articles of Interest
Cryo Sectioning and the Use of Diamond Knives
