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Electron Microscopy Sciences

Diatome Diamond Knives

PDF Downloadarrow13DiATOME Cryo Diamond Knife

cryo diamond knivesThe Low Temperature Ultra-Thin Collection:

Available for wet or dry sectioning with a 25°, 35° or a 45° angle.
Section thickness range 30nm - 1µm. Cutting edge sizes 1.5 - 4mm.

Diatome cryo diamond knives may be used for all low temperature situations such as sectioning of cryo-protected specimens, frozen hydrated specimens and industrial samples such as polymers and rubber.

There are numerous advantages to our cryo diamond knives as opposed to glass knives:

  • They produce thinner sections.
  • Ultrathin and semithin sections with the same knife.
  • Easy to produce long ribbons of sections.
  • Less compression and greater uniformity of sections.
  • You gain time. No knife manufacturing, no knife changing, hence no waiting for temperature equilibration.

arrowCryo 25°

The cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation.

arrowCryo 35° Cryo 45°

The 45° knife is well suited for routine cryo sectioning.

The 35° knife allows you to section with considerable reduction of mechanical damage to the specimen (up to 20% less compression as compared to the 45° knife) and, in turn, better structural preservation of most frozen samples (Ref 6, 7, 8).

The triangular holder for dry sectioning and the boat for the sectioning with a liquid (DMSO/water and others) are made of a special copper/nickel alloy. They both guarantee excellent cold or heat transmission.

The seal is made of a cold-resistant epoxy resin.

cryo diamond knife
Styrene-butadiene block copolymer x 25'000, Ronald Walter, BASF Aktiengesellschaft, Polymer Physics, D-67056 Ludwigshafen.

Cryo Dry

DescriptionAnglemmKnifeCat#PriceOrder
Cryo Dry Diamond Knife 25° 3.0mm new30-CD25 3,500.00Add to Cart
      resharpen30-CD25R 2,195.00 Add to Cart
Cryo Dry Diamond Knife 35° 1.5mm new15-CDL 1,955.00Add to Cart
      exchange15-CDLE 1,350.00Add to Cart
      resharpen15-CDLR 995.00Add to Cart
Cryo Dry Diamond Knife 35° 1.8mm new18-CDL 2,400.00Add to Cart
      exchange18-CDLE 1,450.00 Add to Cart
      resharpen18-CDLR 1,200.00 Add to Cart
Cryo Dry Diamond Knife 35° 2.1mm new21-CDL 2,418.00 Add to Cart
      exchange21-CDLE 1,695.00 Add to Cart
      resharpen21-CDLR 1,390.00 Add to Cart
Cryo Dry Diamond Knife 35° 2.4mm new24-CDL 2,800.00 Add to Cart
      exchange24-CDLE 1,900.00 Add to Cart
      resharpen24-CDLR 1,595.00 Add to Cart
Cryo Dry Diamond Knife 35° 2.5mm new27-CDL 2,800.00 Add to Cart
      exchange27-CDLE 1,940.00 Add to Cart
      resharpen27-CDLR 1,675.00 Add to Cart
Cryo Dry Diamond Knife 35° 3.0mm new30-CDL 3,285.00 Add to Cart
      exchange30-CDLE 2,300.00 Add to Cart
      resharpen30-CDLR 1,985.00 Add to Cart
Cryo Dry Diamond Knife 35° 3.5mm new35-CDL 3,680.00 Add to Cart
      exchange35-CDLE 2,630.00 Add to Cart
      resharpen35-CDLR 2,300.00 Add to Cart
Cryo Dry Diamond Knife 35° 4.0mm new40-CDL 4,085.00 Add to Cart
      exchange40-CDLE 2,900.00 Add to Cart
      resharpen40-CDLR 2,550.00 Add to Cart
Cryo Dry Diamond Knife 45° 1.5mm new15-CDS 1,995.00 Add to Cart
      exchange15-CDSE 1,350.00 Add to Cart
      resharpen15-CDSR 995.00 Add to Cart
Cryo Dry Diamond Knife 45° 1.8mm new18-CDS 2,400.00 Add to Cart
      exchange18-CDSE 1,450.00 Add to Cart
      resharpen18-CDSR 1,200.00 Add to Cart
Cryo Dry Diamond Knife 45° 2.1mm new21-CDS 2,418.00 Add to Cart
      exchange21-CDSE 1,695.00 Add to Cart
      resharpen21-CDSR 1,390.00 Add to Cart
Cryo Dry Diamond Knife 45° 2.4mm new24-CDS 2,800.00 Add to Cart
      exchange24-CDSE 1,900.00 Add to Cart
      resharpen24-CDSR 1,595.00 Add to Cart
Cryo Dry Diamond Knife 45° 2.5mm new27-CDS 2,800.00 Add to Cart
      exchange27-CDSE 1,940.00 Add to Cart
      resharpen27-CDSR 1,675.00 Add to Cart
Cryo Dry Diamond Knife 45° 3.0mm new30-CDS 3,285.00 Add to Cart
      exchange30-CDSE 2,300.00 Add to Cart
      resharpen30-CDSR 1,985.00 Add to Cart
Cryo Dry Diamond Knife 45° 3.5mm new35-CDS 3,680.00 Add to Cart
      exchange35-CDSE 2,630.00 Add to Cart
      resharpen35-CDSR 2,300.00 Add to Cart
Cryo Dry Diamond Knife 45° 4.0mm new40-CDS 4,085.00 Add to Cart
      exchange40-CDSE 2,900.00 Add to Cart
      resharpen40-CDSR 2,550.00 Add to Cart

Cryo Wet

DescriptionAnglemmKnifeCat#PriceOrder
Cryo Wet Diamond Knife 35° 1.5mm new15-CWL 1,995.00 Add to Cart
     exchange15-CWLE 1,350.00 Add to Cart
     resharpen15-CWLR 995.00 Add to Cart
Cryo Wet Diamond Knife 35° 1.8mm new18-CWL 2,400.00 Add to Cart
     exchange18-CWLE 1,450.00 Add to Cart
     resharpen18-CWLR 1,200.00 Add to Cart
Cryo Wet Diamond Knife 35° 2.1mm new21-CWL 2,418.00 Add to Cart
     exchange21-CWLE 1,695.00 Add to Cart
     resharpen21-CWLR 1,390.00 Add to Cart
Cryo Wet Diamond Knife 35° 2.4mm new24-CWL 2,800.00 Add to Cart
     exchange24-CWLE 1,900.00 Add to Cart
     resharpen24-CWLR 1,595.00 Add to Cart
Cryo Wet Diamond Knife 35° 2.5mm new27-CWL 2,800.00 Add to Cart
     exchange27-CWLE 1,940.00 Add to Cart
     resharpen27-CWLR 1,675.00 Add to Cart
Cryo Wet Diamond Knife 35° 3.0mm new30-CWL 3,285.00 Add to Cart
     exchange30-CWLE 2,300.00 Add to Cart
     resharpen30-CWLR 1,985.00 Add to Cart
Cryo Wet Diamond Knife 35° 3.5mm new35-CWL 3,680.00 Add to Cart
     exchange35-CWLE 2,630.00 Add to Cart
     resharpen35-CWLR 2,300.00 Add to Cart
Cryo Wet Diamond Knife 35° 4.0mm new40-CWL 4,085.00 Add to Cart
     exchange40-CWLE 2,900.00 Add to Cart
     resharpen40-CWLR 2,550.00 Add to Cart
Cryo Wet Diamond Knife 45° 1.5mm new15-CWS 1,955.00 Add to Cart
     exchange15-CWSE 1,350.00 Add to Cart
     resharpen15-CWSR 995.00 Add to Cart
Cryo Wet Diamond Knife 45° 1.8mm new18-CWS 2,400.00 Add to Cart
     exchange18-CWSE 1,450.00 Add to Cart
     resharpen18-CWSR 1,200.00 Add to Cart
Cryo Wet Diamond Knife 45° 2.1mm new21-CWS 2,418.00 Add to Cart
     exchange21-CWSE 1,695.00 Add to Cart
     resharpen21-CWSR 1,390.00 Add to Cart
Cryo Wet Diamond Knife 45° 2.4mm new24-CWS 2,800.00 Add to Cart
     exchange24-CWSE 1,900.00 Add to Cart
     resharpen24-CWSR 1,595.00 Add to Cart
Cryo Wet Diamond Knife 45° 2.5mm new27-CWS 2,800.00 Add to Cart
     exchange27-CWSE 1,940.00 Add to Cart
     resharpen27-CWSR 1,675.00 Add to Cart
Cryo Wet Diamond Knife 45° 3.0mm new30-CWS 3,285.00 Add to Cart
     exchange30-CWSE 2,300.00 Add to Cart
     resharpen30-CWSR 1,985.00 Add to Cart
Cryo Wet Diamond Knife 45° 3.5mm new35-CWS 3,680.00 Add to Cart
     exchange35-CWSE 2,630.00 Add to Cart
     resharpen35-CWSR 2,300.00 Add to Cart
Cryo Wet Diamond Knife 45° 4.0mm new40-CWS 4,085.00 Add to Cart
     exchange40-CWSE 2,900.00 Add to Cart
     resharpen40-CWSR 2,550.00 Add to Cart

References

A. Al-Amoudi, D. Studer and J. Dubochet: Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy. Journal of Structural Biology 150, pp. 109-121, 2005.

W. Liu, H. J. Geuze, J. W. Slot: Improving structural integrity of cryosections for immunogold labeling Histochemistry and Cell Biohgy, Vol. 106, PP. 41-55, 1996.

M. Michel, H. Gnagi and M. Muller: Diamonds are a cryosectioner's best friend. Journal of Microscopy, Vol 166, Pt 1, 43-56, 1992.

P. J. Peters: Cryo-lmmunogold Electron Microscopy, In Current Protocols in Cell Biology {J.S. Bonifacino, M. Dasso, J.B. Harford, J. Lippincott-Schwartz and K.M. Yamada, eds) pp. 4.7.1-4.7.12, 1999. Jonn Wiley & Sons, New York.

K. Richter: Cutting artifacts on ultrathin cryosections of biological bulk specimens. Micron, Vol. 25, No. 4, pp. 297-308. 1994.

P. Zhang, E. Bos, J. Heymann, H. Gnaegi, M. Kessel, P.J. Peters, S. Subramaniam: Direct visualisation of receptor arrays in frozen-hydrated sections and plungefrozen specimens of E.coli engineered to overproduce the chemotaxis receptor Tsr. Journal of Microscopy, Vol. 216, Pt 1, pp. 76-83, 2004.

What services can we offer you?

  • Technical assistance in all fields of ultramicrotomy.
  • Free sectioning tests for all types of samples.
  • Make use of our many years of experience in perfecting our knives.
  • With a telephone call, an e-mail or a fax message we can inform you of any details you require.

We are looking forward to hearing from you soon!

Your DiATOME Team

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