DiATOME Cryo Diamond Knife
The Low Temperature Ultra-Thin Collection:
Available for wet or dry sectioning with a 25°, 35° or a 45° angle.
Section thickness range 30nm - 1µm. Cutting edge sizes 1.5 - 4mm.
Diatome cryo diamond knives may be used for all low temperature situations such as sectioning of cryo-protected specimens, frozen hydrated specimens and industrial samples such as polymers and rubber.
There are numerous advantages to our cryo diamond knives as opposed to glass knives:
- They produce thinner sections.
- Ultrathin and semithin sections with the same knife.
- Easy to produce long ribbons of sections.
- Less compression and greater uniformity of sections.
- You gain time. No knife manufacturing, no knife changing, hence no waiting for temperature equilibration.
Cryo 25°
The cryo 25° knife is designed for sectioning frozen hydrated specimens. The 25° angle results in the least possible compression and the best structure preservation.
Cryo 35°
Cryo 45°
The 45° knife is well suited for routine cryo sectioning.
The 35° knife allows you to section with considerable reduction of mechanical damage to the specimen (up to 20% less compression as compared to the 45° knife) and, in turn, better structural preservation of most frozen samples (Ref 6, 7, 8).
The triangular holder for dry sectioning and the boat for the sectioning with a liquid (DMSO/water and others) are made of a special copper/nickel alloy. They both guarantee excellent cold or heat transmission.
The seal is made of a cold-resistant epoxy resin.
Styrene-butadiene block copolymer x 25'000, Ronald Walter, BASF Aktiengesellschaft, Polymer Physics, D-67056 Ludwigshafen.
Cryo Dry
| Description | Angle | mm | Knife | Cat# | Price | Order |
| Cryo Dry Diamond Knife | 25° | 3.0mm | new | 30-CD25 | 3,500.00 | Add to Cart |
| resharpen | 30-CD25R | 2,195.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 35° | 1.5mm | new | 15-CDL | 1,955.00 | Add to Cart |
| exchange | 15-CDLE | 1,350.00 | Add to Cart | |||
| resharpen | 15-CDLR | 995.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 35° | 1.8mm | new | 18-CDL | 2,400.00 | Add to Cart |
| exchange | 18-CDLE | 1,450.00 | Add to Cart | |||
| resharpen | 18-CDLR | 1,200.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 35° | 2.1mm | new | 21-CDL | 2,418.00 | Add to Cart |
| exchange | 21-CDLE | 1,695.00 | Add to Cart | |||
| resharpen | 21-CDLR | 1,390.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 35° | 2.4mm | new | 24-CDL | 2,800.00 | Add to Cart |
| exchange | 24-CDLE | 1,900.00 | Add to Cart | |||
| resharpen | 24-CDLR | 1,595.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 35° | 2.5mm | new | 27-CDL | 2,800.00 | Add to Cart |
| exchange | 27-CDLE | 1,940.00 | Add to Cart | |||
| resharpen | 27-CDLR | 1,675.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 35° | 3.0mm | new | 30-CDL | 3,285.00 | Add to Cart |
| exchange | 30-CDLE | 2,300.00 | Add to Cart | |||
| resharpen | 30-CDLR | 1,985.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 35° | 3.5mm | new | 35-CDL | 3,680.00 | Add to Cart |
| exchange | 35-CDLE | 2,630.00 | Add to Cart | |||
| resharpen | 35-CDLR | 2,300.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 35° | 4.0mm | new | 40-CDL | 4,085.00 | Add to Cart |
| exchange | 40-CDLE | 2,900.00 | Add to Cart | |||
| resharpen | 40-CDLR | 2,550.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 45° | 1.5mm | new | 15-CDS | 1,995.00 | Add to Cart |
| exchange | 15-CDSE | 1,350.00 | Add to Cart | |||
| resharpen | 15-CDSR | 995.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 45° | 1.8mm | new | 18-CDS | 2,400.00 | Add to Cart |
| exchange | 18-CDSE | 1,450.00 | Add to Cart | |||
| resharpen | 18-CDSR | 1,200.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 45° | 2.1mm | new | 21-CDS | 2,418.00 | Add to Cart |
| exchange | 21-CDSE | 1,695.00 | Add to Cart | |||
| resharpen | 21-CDSR | 1,390.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 45° | 2.4mm | new | 24-CDS | 2,800.00 | Add to Cart |
| exchange | 24-CDSE | 1,900.00 | Add to Cart | |||
| resharpen | 24-CDSR | 1,595.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 45° | 2.5mm | new | 27-CDS | 2,800.00 | Add to Cart |
| exchange | 27-CDSE | 1,940.00 | Add to Cart | |||
| resharpen | 27-CDSR | 1,675.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 45° | 3.0mm | new | 30-CDS | 3,285.00 | Add to Cart |
| exchange | 30-CDSE | 2,300.00 | Add to Cart | |||
| resharpen | 30-CDSR | 1,985.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 45° | 3.5mm | new | 35-CDS | 3,680.00 | Add to Cart |
| exchange | 35-CDSE | 2,630.00 | Add to Cart | |||
| resharpen | 35-CDSR | 2,300.00 | Add to Cart | |||
| Cryo Dry Diamond Knife | 45° | 4.0mm | new | 40-CDS | 4,085.00 | Add to Cart |
| exchange | 40-CDSE | 2,900.00 | Add to Cart | |||
| resharpen | 40-CDSR | 2,550.00 | Add to Cart |
Cryo Wet
| Description | Angle | mm | Knife | Cat# | Price | Order |
| Cryo Wet Diamond Knife | 35° | 1.5mm | new | 15-CWL | 1,995.00 | Add to Cart |
| exchange | 15-CWLE | 1,350.00 | Add to Cart | |||
| resharpen | 15-CWLR | 995.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 35° | 1.8mm | new | 18-CWL | 2,400.00 | Add to Cart |
| exchange | 18-CWLE | 1,450.00 | Add to Cart | |||
| resharpen | 18-CWLR | 1,200.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 35° | 2.1mm | new | 21-CWL | 2,418.00 | Add to Cart |
| exchange | 21-CWLE | 1,695.00 | Add to Cart | |||
| resharpen | 21-CWLR | 1,390.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 35° | 2.4mm | new | 24-CWL | 2,800.00 | Add to Cart |
| exchange | 24-CWLE | 1,900.00 | Add to Cart | |||
| resharpen | 24-CWLR | 1,595.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 35° | 2.5mm | new | 27-CWL | 2,800.00 | Add to Cart |
| exchange | 27-CWLE | 1,940.00 | Add to Cart | |||
| resharpen | 27-CWLR | 1,675.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 35° | 3.0mm | new | 30-CWL | 3,285.00 | Add to Cart |
| exchange | 30-CWLE | 2,300.00 | Add to Cart | |||
| resharpen | 30-CWLR | 1,985.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 35° | 3.5mm | new | 35-CWL | 3,680.00 | Add to Cart |
| exchange | 35-CWLE | 2,630.00 | Add to Cart | |||
| resharpen | 35-CWLR | 2,300.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 35° | 4.0mm | new | 40-CWL | 4,085.00 | Add to Cart |
| exchange | 40-CWLE | 2,900.00 | Add to Cart | |||
| resharpen | 40-CWLR | 2,550.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 45° | 1.5mm | new | 15-CWS | 1,955.00 | Add to Cart |
| exchange | 15-CWSE | 1,350.00 | Add to Cart | |||
| resharpen | 15-CWSR | 995.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 45° | 1.8mm | new | 18-CWS | 2,400.00 | Add to Cart |
| exchange | 18-CWSE | 1,450.00 | Add to Cart | |||
| resharpen | 18-CWSR | 1,200.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 45° | 2.1mm | new | 21-CWS | 2,418.00 | Add to Cart |
| exchange | 21-CWSE | 1,695.00 | Add to Cart | |||
| resharpen | 21-CWSR | 1,390.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 45° | 2.4mm | new | 24-CWS | 2,800.00 | Add to Cart |
| exchange | 24-CWSE | 1,900.00 | Add to Cart | |||
| resharpen | 24-CWSR | 1,595.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 45° | 2.5mm | new | 27-CWS | 2,800.00 | Add to Cart |
| exchange | 27-CWSE | 1,940.00 | Add to Cart | |||
| resharpen | 27-CWSR | 1,675.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 45° | 3.0mm | new | 30-CWS | 3,285.00 | Add to Cart |
| exchange | 30-CWSE | 2,300.00 | Add to Cart | |||
| resharpen | 30-CWSR | 1,985.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 45° | 3.5mm | new | 35-CWS | 3,680.00 | Add to Cart |
| exchange | 35-CWSE | 2,630.00 | Add to Cart | |||
| resharpen | 35-CWSR | 2,300.00 | Add to Cart | |||
| Cryo Wet Diamond Knife | 45° | 4.0mm | new | 40-CWS | 4,085.00 | Add to Cart |
| exchange | 40-CWSE | 2,900.00 | Add to Cart | |||
| resharpen | 40-CWSR | 2,550.00 | Add to Cart |
References
A. Al-Amoudi, D. Studer and J. Dubochet: Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy. Journal of Structural Biology 150, pp. 109-121, 2005.
W. Liu, H. J. Geuze, J. W. Slot: Improving structural integrity of cryosections for immunogold labeling Histochemistry and Cell Biohgy, Vol. 106, PP. 41-55, 1996.
M. Michel, H. Gnagi and M. Muller: Diamonds are a cryosectioner's best friend. Journal of Microscopy, Vol 166, Pt 1, 43-56, 1992.
P. J. Peters: Cryo-lmmunogold Electron Microscopy, In Current Protocols in Cell Biology {J.S. Bonifacino, M. Dasso, J.B. Harford, J. Lippincott-Schwartz and K.M. Yamada, eds) pp. 4.7.1-4.7.12, 1999. Jonn Wiley & Sons, New York.
K. Richter: Cutting artifacts on ultrathin cryosections of biological bulk specimens. Micron, Vol. 25, No. 4, pp. 297-308. 1994.
P. Zhang, E. Bos, J. Heymann, H. Gnaegi, M. Kessel, P.J. Peters, S. Subramaniam: Direct visualisation of receptor arrays in frozen-hydrated sections and plungefrozen specimens of E.coli engineered to overproduce the chemotaxis receptor Tsr. Journal of Microscopy, Vol. 216, Pt 1, pp. 76-83, 2004.
What services can we offer you?
- Technical assistance in all fields of ultramicrotomy.
- Free sectioning tests for all types of samples.
- Make use of our many years of experience in perfecting our knives.
- With a telephone call, an e-mail or a fax message we can inform you of any details you require.
We are looking forward to hearing from you soon!
