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Electron Microscopy Sciences

Diatome Diamond Knives

PDF Downloadarrow13Histology Diamond Knives

arrowHisto and Histo Jumbo

Histo Diamond KnivesFor Room Temperature

Knives are available with a 45° angle.

Section thickness range 0.2 - 5µm.

Cutting edge length 4, 6, and 8mm.

The knife is designed for the sectioning of hard and soft biological and industrial materials, non embedded or embedded in methacrylate or epoxy resins.

The histo knife may be used on all ultramicrotomes and microtomes with a retraction of the specimen in the return phase.

For the production of our histo knives we use natural diamonds of highest quality. They guarantee the best quality and durability of the cutting edges.

Some of the advantages of our histo diamond knife as compared to glass knives are as follows :

  • Perfect sections, free of scores or compression.
  • Serial sections without knife change.
  • Thinner sections.
  • Extreme durability of the cutting edge.
  • No knife making procedure.
  • Lifetime Guarantee.

For 3D reconstruction it is imperative not to lose a single section (Ref. Blumer).

The large Jumbo boat as well as the adhesive (Pattex compact by Henkel) applied to the side of the sample block increase the distinct advantages

They allow:

  • Easy production of section ribbons (0.5-2µm)
  • No section loss
  • No folding
  • The same orientation of all sections
  • Easy collection of section ribbons
  • Multiple ribbons on one glass slide
  • Perfect for Immuno-histo-chemistry

diamond knife section of Nondecalcified rat bone
Nondecalcified rat bone., Scale: 35 mm = 100µm. Daniel Studer, Anatomisches Institut, Bern.

Histo Diamond KnivesarrowHisto Cryo

For Cryo Temperatures

Knives are available in 45° angle.

Section thickness range 0.2 - 5µm.

Cutting edge length 4, 6 and 8mm.

The histo-cryo knives are delivered in boats (for wet cryo sectioning) and triangular holders (for dry cryo sectioning), made of a special copper/nickel alloy, which guarantees the best heat/cold transmission.

DescriptionAnglemmKnifeCat#PriceOrder
Histo Diamond Knife 45° 4.0mm new40-HIS 1,399.00Add to Cart
      exchange40-HISE 975.00Add to Cart
      resharpen40-HISR 700.00Add to Cart
Histo Diamond Knife 45° 6.0mm new60-HIS 2,275.00Add to Cart
      exchange60-HISE 1,650.00Add to Cart
      resharpen60-HISR 1,100.00 Add to Cart
Histo Diamond Knife 45° 8.0mm new80-HIS 2,900.00 Add to Cart
      exchange80-HISE 2,150.00 Add to Cart
      resharpen80-HISR 1,275.00 Add to Cart
Histo Cryo Dry Diamond Knife 45° 4.0mm new40-HDC 1,399.00 Add to Cart
      resharpen40-HDCR 700.00 Add to Cart
Histo Cryo Wet Diamond Knife 45° 4.0mm new40-HWC 1,399.00 Add to Cart
      resharpen40-HWCR 700.00 Add to Cart
Histo Cryo Dry Diamond Knife 45° 6.0mm new60-HDC 2,275.00 Add to Cart
      resharpen60-HDCR 1,300.00 Add to Cart
Histo Cryo Wet Diamond Knife 45° 6.0mm new60-HWC 2,275.00 Add to Cart
      resharpen60-HWCR 1,300.00 Add to Cart
Histo Cryo Dry Diamond Knife 45°  8.0mm  new 80-HDC 2,900.00 Add to Cart
      resharpen 80-HDCR 1,275.00 Add to Cart
Histo Cryo Wet Diamond Knife 45°  8.0mm  new 80-HWC 2,900.00 Add to Cart
      resharpen 80-HWCR 1,275.00 Add to Cart
Jumbo Histo Diamond Knife 45° 6.0mm new60-HISJ 2,425.00 Add to Cart
      exchange60-HISJE 1,675.00 Add to Cart
      resharpen60-HISJR 1,300.00 Add to Cart
Jumbo Histo Diamond Knife 45° 8.0mm new80-HISJ 3,000.00 Add to Cart
      exchange80-HISJE 2,225.00 Add to Cart
      resharpen80-HISJR 1,275.00 Add to Cart

diamond knife
E. B. Hunziker M. E. Müller Institute for Biomechanics,
University of Bern.
Rabbit joint, calcified cartilage/bone. X760 

References

O.L. Reymond: The «semi» diamond knife: a substitute for glass or conventional diamond knives in the ultramicrotomy of thin and semi-thin sections. Bas. Appl. Histochem. 30, pp. 487-494, 1986.

M.J.F. Blumer, P. Gahleitner, T. Narzt, C. Handl, B. Ruthensteiner: Strips of semi-thin sections: an advanced method with a new type of diamond knife. Journal of Neuroscience Methods 120,pp. 11-16, 2002. Contact adress M.J.F. Blumer: michael.blumer@uibk.ac.at

M.J.F. Blumer: Development of a unique eye: photoreceptors of the pelagic predator Atlanta peroni (Gastropoda, Heteropoda). Zoomorphology 119, pp. 81-91, 1996.

What services can we offer you?

  • Technical assistance in all fields of ultramicrotomy.
  • Free sectioning tests for all types of samples.
  • Make use of our many years of experience in perfecting our knives.
  • With a telephone call, an e-mail or a fax message we can inform you of any details you require.

We are looking forward to hearing from you soon!

Your DiATOME Team

AFM Diamond Knife for Ultra and Cryo arrow13arrow13