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Electron Microscopy Sciences

Diatome Diamond Knives

PDF Downloadarrow13DiATOME Diamond Knife Accessories

Brochuremicro manipulator

section ribbon attached to manipulatorA section ribbon attached to a fibre is depicted emerging from a diamond knife edge (left) beneath the ribbon the grid attached to a holder touches the section ribbon.

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For easily handling and mounting of section ribbons. The mounting of the manipulator is easy. The original plate on the cryochamber is removed, the manipulator is fixed in 5 minutes.

Applications

  • Frozen hydrated biological samples (CEMOVIS)
  • Room temperature sectioning of water sensitive samples
    • Dry resin sectioning of biological samples for chemical analysis
    • Dry sectioning of industrial samples such as polymers

Functionality

  • The left manipulator moves a conductive fibre on which the sections are attached by electrostatic force; the growing ribbon is guided.
  • The right manipulator guides the grid very precisely below the section ribbon, the sections are attached to the carbon film with electrostatic force.

Compatibility

  • Leica cryochambers FC6 and FC7 equipped with a Crion ionizer/charger
  • adaption on other cryochambers upon request

Ordering Information

Cat# Description Qty Price Order
DZ100-01 DiATOME manip each 14,250.00 Add to Cart


cryosection

A cryosection showing vacuole, mitochondria and filopodium of a cultured cell. C. Peitsch and I. Iavocache, Inst. of Anatomy, Bern. Bar 100 nm

References

The cryosectioning approach is described by Struder et al.:

Struder, D., Klein A., Iacovache, I, Gnaegi, H., and Zuber B. (2014). A new tool based on two micromanipulators facilitates the handling of ultrathin cryosection ribbons. Journal of Structural Biology, 185, 125-128.

What services can we offer you?

  • Technical assistance in all fields of ultramicrotomy.
  • Free sectioning tests for all types of samples.
  • Make use of our many years of experience in perfecting our knives.
  • With a telephone call, an e-mail or a fax message we can inform you of any details you require.

We are looking forward to hearing from you soon!

Your DiATOME Team

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