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Electron Microscopy Sciences

Grids for Electron Microscopy

Downloadable PDFarrow13QUANTIFOIL® – Holey Carbon Films

QuantifoilQUANTIFOIL® is a perforated support foil with pre-defined hole size, shape and arrangement. It has advantages in electron microscopy (EM) or low-energy electron point source (LEEPS) microscopy when compared with conventional holey film.

QUANTIFOIL® is offered with circular and square, orthogonal arranged holes. Films with different hole sizes and bar widths are available. Carbon is the standard material that makes the foil.

QUANTIFOIL® is a superior quality of holey carbon film, which facilitates the use of automation in TEM. (e.g. The National Resource for Automated Molecular Microscopy, at the Scripps Research Institute, has developed a system, called Leginon, for automatically acquiring images from a transmission electron microscope).

QUANTIFOIL R 2/2The surface properties of QUANTIFOIL® holey carbon support film, especially the wetting properties, may have to be adapted according to one's particular requirements. Untreated aging QUANTIFOIL® tends to be hydrophobic. Hydrophillicity of the foil can be achieved by glow discharging in residual air or by metal coating.

QUANTIFOIL® in low-energy electron point source (LEEPS) microscopy. Quantifoil® with a regular pattern is required in order to be able to distinguish an object, which is spanned over a hole. An object cannot be discriminated from the support in the case of conventional holey support film. (H.W. Fink & C. Schonenberger, University of Basel, used QUANTIFOIL® for the measurement of electrical current through DNA molecules.)

The foil is ~12nm thick and mounted on either copper, nickel or gold grids with either square or round holes of different sizes.

Holey films with 2µ round holes are used at magnifications between 30,000x and 40,000x.

QUANTIFOIL® with Circular Holes

QUANTIFOIL® with circular holes is mainly used in cryo-EM. The roundness of the holes is advantageous with respect to the formation of an ice layer of constant thickness.

The hole size that is chosen depends on the magnification used, and on whether or not one wishes to include support film in the image. The latter makes assessment of the image quality easier, because the power spectrum of a foil is stronger than that of unsupported ice.

The QUANTIFOIL® types with small holes (e.g. 1.2µm) were designed for magnifications around and higher 50,000x. At magnifications between 30,000x and 40,000x we recommend the QUANTIFOIL® types with 2µm holes. Larger holes (e.g. 3.5µm, 5µm) are suitable at magnification around 20,000x.

QUANTIFOIL® R 3.5/1 may be preferred over foils with smaller holes if the carbon film should be outside the frame of the image. This option can be desirable in the case of extended objects, such as filamentous objects, for example. QUANTIFOIL 3.5
QUANTIFOIL® R 1/4 may be preferred over R 1.2/1.3, when an increased tolerance with respect to the position of beam, and a larger beam diameter are desired, such as in the case of automated image acquisition. QUANTIFOIL 1/4
QUANTIFOIL® R 0.6/1 Hole size is 0.5µ. Space between holes is 1µ. Center to center is 1.6µ (hole size may be as large as 1µ).  
QUANTIFOIL® R 5/20 Hole size is 5µ. Space between holes is 20µ. Center to center is 25µ.  
QUANTIFOIL® R 2/4 may be preferred over R 2/2, when an increased tolerance with respect to the position of beam, and a larger beam diameter are desired, such as in the case of automated image acquisition. QUANTIFOIL R 2/4
QUANTIFOIL® R 2/2 Holey films with 2µm circular holes are used at magnifications between 30,000x and 40,000x. Quantifoil
QUANTIFOIL® R 2/1 has more open area than R 2/2. It is used when focusing is carried out on the edge of a hole burnt in the ice in a neighboring hole instead of on the foil adjacent to the hole.
QUANTIFOIL R 2/1
QUANTIFOIL® R 1.2/1.3 A foil with ~1.2µm circular holes and a spacing of ~2.5µm between the holes. This type is used at magnifications around 50,000x. QUANTIFOIL 1.2

QUANTIFOIL® with Square Holes

QUANTIFOIL® with square holes and relatively narrow bars can be used in electron microscopy to support a thin carbon film, which by itself is too fragile to span a grid square. The QUANTIFOIL® types with square holes constitute an optimum between a maximum of open area on the one hand, and mechanical stability on the other hand.

QUANTIFOIL® S 7/2 constitutes an optimum between a maximum of open area on the one hand, and mechanical stability on the other hand. QUANTIFOIL S

QUANTIFOIL® with Different Hole Shapes

QUANTIFOIL® Multi A is a holey film, in which a pattern of various hole sizes, shapes and arrangements is repeated. In addition to circular holes, the pattern includes oval-shaped ones which appear round at high tilt angles (~70°). The circular holes have diameters of 1µm, 1.4µm, and 2µm. The elliptical holes measure 1µm x 4µm and 2µm x 8µm (along the shortest and longest axes, respectively). QUANTIFOIL Multi A

QUANTIFOIL® with Hexagonal Geometry

This type of QUANTIFOIL® is meant for slot grids. It was especially designed for supporting serial thin sections. It offers an optimum between mechanical stability on the one hand and background-free area on the other hand. The foil is thin enough to allow those parts of the sections that lie on the bars to be interpreted. In this way, the information in the sections can be interpreted to the maximum.

QUANTIFOIL® Hex 15 A foil with hole size of 26µm (diameter of inscribed circle) and a repeating distance of 41µm, the side length of the holes and the bar width are 15µm. QUANTIFOIL HEXAGONAL

Ordering Information:

QUANTIFOIL® is generally delivered as a carbon foil; it can be reinforced with plastic film. The standard thickness for the carbon foil is 12nm; other thicknesses between 10 and 25nm can be made upon request. The grids are packed with their coated side toward the center of the grid box.

Hole
Shape
Hole Size Period Carbon
Grid Type
Pack Price 200 Mesh 300 Mesh 400 Mesh
R 0.6/1 0.6µ 1.6µ Copper 50/pk 395.00 Add to Cart Add to Cart Add to Cart
R 0.6/1 0.6µ 1.6µ Copper 100/pk 718.00 Add to Cart Add to Cart Add to Cart
R 0.6/1 0.6µ 1.6µ Nickel 50/pk 450.00 Add to Cart Add to Cart Add to Cart
R 0.6/1 0.6µ 1.6µ Nickel 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 0.6/1 0.6µ 1.6µ Gold 50/pk 450.00 Add to Cart Add to Cart Add to Cart
R 0.6/1 0.6µ 1.6µ Gold 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 1/2 Copper 100/pk 718.00 Add to Cart Add to Cart Add to Cart
R 1/2 Nickel 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 1/2 Gold 100/pk 895.00 Add to Cart Add to Cart Add to Cart
R 1/4 Copper 50/pk 395.00 Add to Cart Add to Cart Add to Cart
R 1/4 Copper 100/pk 718.00 Add to Cart Add to Cart Add to Cart
R 1/4 Nickel 50/pk 450.00 Add to Cart Add to Cart Add to Cart
R 1/4 Nickel 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 1/4 Gold 50/pk 450.00 Add to Cart Add to Cart Add to Cart
R 1/4 Gold 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 ~1.2µ 1.3µ Copper 25/pk 190.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 ~1.2µ 1.3µ Copper 50/pk 350.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 ~1.2µ 1.3µ Copper 100/pk 700.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 ~1.2µ 1.3µ Nickel 25/pk 200.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 ~1.2µ 1.3µ Nickel 50/pk 400.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 ~1.2µ 1.3µ Nickel 100/pk 785.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 ~1.2µ 1.3µ Gold 25/pk 205.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 ~1.2µ 1.3µ Gold 50/pk 440.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 ~1.2µ 1.3µ Gold 100/pk 825.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 1.2µ 2.5µ Copper 100/pk 718.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 1.2µ 2.5µ Nickel 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 1.2µ 2.5µ Gold 100/pk 825.00 Add to Cart Add to Cart Add to Cart
R 1.2/20 1.2µ 21.2µ Copper 100/pk 718.00 Add to Cart Add to Cart Add to Cart
R 1.2/20 1.2µ 21.2µ Nickel 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 1.2/20 1.2µ 21.2µ Gold 100/pk 825.00 Add to Cart Add to Cart Add to Cart
R 2/1 Copper 25/pk 180.00 Add to Cart Add to Cart Add to Cart
R 2/1 Copper 50/pk 372.00 Add to Cart Add to Cart Add to Cart
R 2/1 Copper 100/pk 730.00 Add to Cart Add to Cart Add to Cart
R 2/1 Nickel 25/pk 206.00 Add to Cart Add to Cart Add to Cart
R 2/1 Nickel 50/pk 380.00 Add to Cart Add to Cart Add to Cart
R 2/1 Nickel 100/pk 749.00 Add to Cart Add to Cart Add to Cart
R 2/1 Gold 25/pk 205.00 Add to Cart Add to Cart Add to Cart
R 2/1 Gold 50/pk 440.00 Add to Cart Add to Cart Add to Cart
R 2/1 Gold 100/pk 825.00 Add to Cart Add to Cart Add to Cart
R 2/2 Copper 25/pk 180.00 Add to Cart Add to Cart Add to Cart
R 2/2 Copper 50/pk 350.00 Add to Cart Add to Cart Add to Cart
R 2/2 Copper 100/pk 700.00 Add to Cart Add to Cart Add to Cart
R 2/2 Nickel 25/pk 200.00 Add to Cart Add to Cart Add to Cart
R 2/2 Nickel 50/pk 380.00 Add to Cart Add to Cart Add to Cart
R 2/2 Nickel 100/pk 749.00 Add to Cart Add to Cart Add to Cart
R 2/2 Gold 25/pk 205.00 Add to Cart Add to Cart Add to Cart
R 2/2 Gold 50/pk 420.00 Add to Cart Add to Cart Add to Cart
R 2/2 Gold 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 2/4 Copper 25/pk 180.00 Add to Cart Add to Cart Add to Cart
R 2/4 Copper 50/pk 350.00 Add to Cart Add to Cart Add to Cart
R 2/4 Copper 100/pk 700.00 Add to Cart Add to Cart Add to Cart
R 2/4 Nickel 25/pk 200.00 Add to Cart Add to Cart Add to Cart
R 2/4 Nickel 50/pk 375.00 Add to Cart Add to Cart Add to Cart
R 2/4 Nickel 100/pk 749.00 Add to Cart Add to Cart Add to Cart
R 2/4 Gold 25/pk 250.00 Add to Cart Add to Cart Add to Cart
R 2/4 Gold 50/pk 450.00 Add to Cart Add to Cart Add to Cart
R 2/4 Gold 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 3/3 Copper 100/pk 718.00 Add to Cart Add to Cart Add to Cart
R 3/3 Nickel 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 3/3 Gold 100/pk 895.00 Add to Cart Add to Cart Add to Cart
R 3/5 Copper 100/pk 718.00 Add to Cart Add to Cart Add to Cart
R 3/5 Nickel 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 3/5 Gold 100/pk 895.00 Add to Cart Add to Cart Add to Cart
R 3.5/1 3.5µ 4.5µ Copper 50/pk 395.00 Add to Cart Add to Cart Add to Cart
R 3.5/1 3.5µ 4.5µ Copper 100/pk 718.00 Add to Cart Add to Cart Add to Cart
R 3.5/1 3.5µ 4.5µ Nickel 50/pk 450.00 Add to Cart Add to Cart Add to Cart
R 3.5/1 3.5µ 4.5µ Nickel 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 3.5/1 3.5µ 4.5µ Gold 50/pk 450.00 Add to Cart Add to Cart Add to Cart
R 3.5/1 3.5µ 4.5µ Gold 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 5/10 15µ Copper 100/pk 718.00 Add to Cart Add to Cart Add to Cart
R 5/10 15µ Nickel 100/pk 812.00 Add to Cart Add to Cart Add to Cart
R 5/10 15µ Gold 100/pk 895.00 Add to Cart Add to Cart Add to Cart
R 5/20 25µ Copper 50/pk 700.00 Add to Cart Add to Cart Add to Cart
R 5/20 25µ Copper 100/pk 1,340.00 Add to Cart Add to Cart Add to Cart
R 5/20 25µ Nickel 50/pk 795.00 Add to Cart Add to Cart Add to Cart
R 5/20 25µ Nickel 100/pk 1,510.00 Add to Cart Add to Cart Add to Cart
R 5/20 25µ Gold 50/pk 795.00 Add to Cart Add to Cart Add to Cart
R 5/20 25µ Gold 100/pk 1,510.00 Add to Cart Add to Cart Add to Cart
R 6/6.5 12.5µ Copper 100/pk 895.00 Add to Cart Add to Cart Add to Cart
R 6/6.5 12.5µ Nickel 100/pk 970.00 Add to Cart Add to Cart Add to Cart
R 6/6.5 12.5µ Gold 100/pk 1,000.00 Add to Cart Add to Cart Add to Cart
R 6/100 106µ Copper 100/pk 895.00 Add to Cart Add to Cart Add to Cart
R 6/100 106µ Nickel 100/pk 970.00 Add to Cart Add to Cart Add to Cart
R 6/100 106µ Gold 100/pk 1,000.00 Add to Cart Add to Cart Add to Cart
R 10/5 10µ 15µ Copper 100/pk 895.00 Add to Cart Add to Cart Add to Cart
R 10/5 10µ 15µ Nickel 100/pk 970.00 Add to Cart Add to Cart Add to Cart
R 10/5 10µ 15µ Gold 100/pk 1,000.00 Add to Cart Add to Cart Add to Cart
R 10/10 10µ 20µ Copper 100/pk 895.00 Add to Cart Add to Cart Add to Cart
R 10/10 10µ 20µ Nickel 100/pk 970.00 Add to Cart Add to Cart Add to Cart
R 10/10 10µ 20µ Gold 100/pk 1,000.00 Add to Cart Add to Cart Add to Cart
R 10/20 10µ 30µ Copper 100/pk 895.00 Add to Cart Add to Cart Add to Cart
R 10/20 10µ 30µ Nickel 100/pk 970.00 Add to Cart Add to Cart Add to Cart
R 10/20 10µ 30µ Gold 100/pk 1,000.00 Add to Cart Add to Cart Add to Cart
R 17/5 17.5µ 22.5µ Copper 100/pk 895.00 Add to Cart Add to Cart Add to Cart
R 17/5 17.5µ 22.5µ Nickel 100/pk 970.00 Add to Cart Add to Cart Add to Cart
R 17/5 17.5µ 22.5µ Gold 100/pk 1,000.00 Add to Cart Add to Cart Add to Cart
S 7/2 7x7µ Copper 25/pk 180.00 Add to Cart Add to Cart Add to Cart
S 7/2 7x7µ Copper 50/pk 350.00 Add to Cart Add to Cart Add to Cart
S 7/2 7x7µ Copper 100/pk 700.00 Add to Cart Add to Cart Add to Cart
S 7/2 7x7µ Nickel 25/pk 200.00 Add to Cart Add to Cart Add to Cart
S 7/2 7x7µ Nickel 50/pk 375.00 Add to Cart Add to Cart Add to Cart
S 7/2 7x7µ Nickel 100/pk 749.00 Add to Cart Add to Cart Add to Cart
S 7/2 7x7µ Gold 25/pk 205.00 Add to Cart Add to Cart Add to Cart
S 7/2 7x7µ Gold 50/pk 400.00 Add to Cart Add to Cart Add to Cart
S 7/2 7x7µ Gold 100/pk 795.00 Add to Cart Add to Cart Add to Cart
Multi A Copper 25/pk 180.00 Add to Cart Add to Cart Add to Cart
Multi A Copper 50/pk 350.00 Add to Cart Add to Cart Add to Cart
Multi A Copper 100/pk 700.00 Add to Cart Add to Cart Add to Cart
Multi A Nickel 25/pk 180.00 Add to Cart Add to Cart Add to Cart
Multi A Nickel 50/pk 375.00 Add to Cart Add to Cart Add to Cart
Multi A Nickel 100/pk 749.00 Add to Cart Add to Cart Add to Cart

Hole
Shape
Hole Size Description Grid Type Pack Price  
HEX 15 26µ 0.5 x 2mm slot grids Copper 50/pk 450.00 Add to Cart
HEX 15 26µ 0.5 x 2mm slot grids Copper 100/pk 850.00 Add to Cart

arrow13QUANTIFOIL® – SiO2

The currently favored and already established material other than carbon is SiO2.

Ordering Information:

Hole
Shape
Hole Size Period SiO2
Grid Type
Pack Price 200 Mesh 300 Mesh 400 Mesh
R 1/4 Copper 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 1/4 Nickel 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 1/4 Gold 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 ~1.2µ 1.3µ Copper 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 ~1.2µ 1.3µ Nickel 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 1.2µ 2.5µ Copper 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 1.2/1.3 1.2µ 2.5µ Nickel 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 1.2/20 1.2µ 21.2µ Copper 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 1.2/20 1.2µ 21.2µ Nickel 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 2/2 Copper 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 2/2 Nickel 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 2/2 Gold 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 2/4 Copper 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 2/4 Nickel 100/pk 793.00 Add to Cart Add to Cart Add to Cart
R 2/4 Gold 100/pk 793.00 Add to Cart Add to Cart Add to Cart

UltrAuFoil® Holey Gold Films arrow13arrow13