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Electron Microscopy Sciences

Histology and Light Microscopy

arrow13Prepared Staining Kits for Histology and Pathology

C. BONES AND CALCIUM STAINS

Alcoholic Stain SolutionsarrowCanaliculi and Lacunae Stain

Powers, Rasmussen and Clark (1951)

For bones and teeth.

Stain results:

Canaliculi, lacunae, odontoblast and
dentinal tubules:
  Bluish to purplish black

References:

  • Powers, M.M., Rasmusen, G.L., and Clark, G.,Anat. Rec., 111:117-6, (1951)
  • Clark, G. (ed), Staining Procedures, 3rd edition, William & Wilkins, Baltimore, c 1973, p. 136
  • Bodian, D., Anat. Rec., 65:89-97, 1936
  • Bodian, D., Anat. Rec., 69:153-62, 1937
RT 26201-01 Cupric Nitrate, 1% Aqueous msds 500 ml 17.00 Add to Cart
RT 26201-02 Protargol Solution, 1% Aqueous msds 500 ml 25.00 Add to Cart
RT 26201-03 Copper Shot msds 50 g 24.00 Add to Cart
RT 26201-04 Hydroquinone Reducing Solution msds 500 ml 21.00 Add to Cart
RT 26201-05 Gold Chloride, 1% Aqueous msds 250 ml 210.00 Add to Cart
RT 26201-06 Oxalic Acid, 2% Aqueous msds 500 ml 24.00 Add to Cart
RT 26201-07 Sodium Thiosulfate, 5% Aqueous msds 500 ml 20.00 Add to Cart

arrowModified Alizarin Red S for Fetal Specimens

Cumley, Crow, and Griffin (1939)

For Minute bones and fetal ossification in mammalian embryos

Stain results:

Bone:   Red
Soft Tissue: .   Transparent and unstained

References:

  • Cumley, R.W., Crow, J.F., and Griffin, A.B., Stain Techn., 14:7-11, 1939
  • Dawson, A.B., Stain Techn., 1:123-124, 1926
  • Richmond, G.W. and Bennett, L., Stain Techn., 13:77-79, 1938
  • Clark, G., (ed), Staining Procedures, 3rd ed., William & Wilkins, Baltimore, p. 138, c. 1973
RT 26204-01 Alizarin S, 0.01% Aqueous msds 500 ml 22.00 Add to Cart
RT 26204-02 Potassium Hydroxide, 1% Aqueous msds 500 ml 21.00 Add to Cart
RT 26204-03 Potassium Hydroxide, 10% Aqueous   500 ml 22.00 Add to Cart
RT 26204-04 Glycerol-Potassium Hydroxide   500 ml 22.00 Add to Cart

Alcoholic Stain SolutionsarrowAlizarin Red S and Toluidine Blue O

Williams (1941); Dawson (1926)

Distinction between bone and cartilage in mammalian embryos.

Stain results:

Soft Tissues:   Transparent
Osseous Tissues:   Deep Red
Cartilage:   Dark Blue

References:

  • Dawson A.B., Stain Techn., 1:123-4, 1926
  • Williams, T.W., Stain Techn., 16:23-5, 1941
  • Clark, G., (ed), Staining Procedures, 3rd Ed., Williams & Wilkins Baltimore, p. 139, c. 1973
RT 26205-01 Alizarin Red S, 0.02% Aqueous msds 500 ml 22.00 Add to Cart
RT 26205-1A Potassium Hydroxide, 4%, Aqueous   500 ml 23.00 Add to Cart
RT 26205-02 Toluidine Blue O, 0.25% Aqueous msds 500 ml 35.00 Add to Cart
RT 26205-03 Ammonium Alcohol   500 ml 24.00 Add to Cart
RT 26205-05 Potassium Hydroxide, 2%, Aqueous msds 500 ml 24.00 Add to Cart
RT 26205-06 Sulfuric Acid Alcohol msds 500 ml 20.00 Add to Cart

Alcoholic Stain SolutionsarrowAlizarin Red S for Calcium Deposits

Calcium deposits stain.

Stain Results:

Calcium Sites:   Covered and surrounded by a heavy orange
red precipitate which is birefringent between
crossed polaroids
Background:   Faint Pink

References:

  • McGee-Russell, S.M., J. Histochem., 6:22-42, 1958.
  • Clark, G., (ed), Staining Procedures, 3rd d., Williams & Wilkins, Baltimore, p. 140, c. 1973.
RT 26206-01 Alizarin S, 2% Solution, pH 4.2 msds 250 ml 17.00 Add to Cart
RT 26206-02 Reagent Alcohol, 50% msds 500 ml 15.00 Add to Cart
RT 26206-03 Acetone msds 1 qt 14.00 Add to Cart
RT 26206-04 Acetone:Xylene, 1:1 msds 500 ml 22.00 Add to Cart

Alcoholic Stain SolutionsarrowPizzolato's Method for Calcium Oxalate

For bone and its related stain.

Stain results:

Calcium oxalate:   Black
All other tissue elements:   Red

References:

  • Pizzolato, P., J. Histochem. Cytochem., 12:333-136, 1964.
  • Luna, L.G., (ed), Manual of Staining Methods of the AFIP, 3rd Ed, McGraw-Hill, NY, c 1968, p 77.
RT 26212-01 Silver Nitrate, 5% Aqueous msds 250 ml 83.00 Add to Cart
RT 26212-02 Hydrogen Peroxide, 30% msds 450 ml 22.00 Add to Cart
RT 26212-03 Nuclear Fast Red (Kernechtrot) Solution msds 500 ml 43.00 Add to Cart

D. BONE MARROW STAINS

Alcoholic Stain SolutionsarrowMay-Grunwald / Giemsa Combined Stain

Beck (1938); Pappanheim (1912)

Stain results:

Nuclei, Bacteria   Blue
Cytoplasm   Pink-Rose

References

  • Beck, R.C., Laboratory Manual of Hematological Technique, W.B. Saunders & Co., Philadelphia, c. 1938, pp 233-236
  • Clark, G. (ed), Staining Procedures, 3rd ed. Williams &Wilkins, Baltimore, c. 1973, pp 131-132
  • Strumia, M.M., J Lab Clin. Med 21:930-934, 1935-1936
  • Luna, L.G., (ed) Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd ed. McGraw-Hill, NY, c. 1968, pp 121-122
RT 26250-01 May-Grunwald Staining Solution
OR
msds 500 ml 32.00 Add to Cart
RT 26250-1A Jenner Staining Solution   500 ml 36.00 Add to Cart
RT 26250-02 Giemsa Staining Solution msds 250 ml 32.00 Add to Cart

Alcoholic Stain SolutionsarrowMaximow's Method for Bone Marrow

Mallory (1961)

Morphology details of marrow cells.

Stain results:

Nuclei   Blue
Basophile leukocyte, mast cell granules   Purple to Violet
Cartilage   Purple
Erythrocytes, eosinophile granules   Pink
Cytoplasm   Blue to Pink

References

  • AFIP Manual of Histologic Staining Methods, 3rd Ed, Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 120
  • Mallory, F.B., Pathological technique, New York, Hafner Publishing Co., 1961, p.196
RT 26252-01 Mayer's Hematoxylin msds 500 ml 44.00 Add to Cart
RT 26252-02 Eosin Y, 0.1%, Aqueous   500 ml 31.00 Add to Cart
RT 26252-03 Azur II, 0.1%, Aqueous   500 ml 38.00 Add to Cart
RT 26252-04 Phosphate Buffer, 0.1M, pH 6.8-7.0 msds 250 ml 19.00 Add to Cart

Carbohydrate and Mucosubstance Stains arrow13arrow13