Prepared Staining Kits for Histology and Pathology
C. BONES AND CALCIUM STAINS
Canaliculi and Lacunae Stain
Powers, Rasmussen and Clark (1951)
For bones and teeth.
Stain results:
| Canaliculi, lacunae, odontoblast and dentinal tubules: |
Bluish to purplish black |
References:
- Powers, M.M., Rasmusen, G.L., and Clark, G.,Anat. Rec., 111:117-6, (1951)
- Clark, G. (ed), Staining Procedures, 3rd edition, William & Wilkins, Baltimore, c 1973, p. 136
- Bodian, D., Anat. Rec., 65:89-97, 1936
- Bodian, D., Anat. Rec., 69:153-62, 1937
| RT | 26201-01 | Cupric Nitrate, 1% Aqueous |  |
500 ml | 17.00 | Add to Cart |
| RT | 26201-02 | Protargol Solution, 1% Aqueous | |
500 ml | 25.00 | Add to Cart |
| RT | 26201-03 | Copper Shot | |
50 g | 24.00 | Add to Cart |
| RT | 26201-04 | Hydroquinone Reducing Solution | |
500 ml | 21.00 | Add to Cart |
| RT | 26201-05 | Gold Chloride, 1% Aqueous | |
250 ml | 210.00 | Add to Cart |
| RT | 26201-06 | Oxalic Acid, 2% Aqueous | |
500 ml | 24.00 | Add to Cart |
| RT | 26201-07 | Sodium Thiosulfate, 5% Aqueous | |
500 ml | 20.00 | Add to Cart |
Modified Alizarin Red S for Fetal Specimens
Cumley, Crow, and Griffin (1939)
For Minute bones and fetal ossification in mammalian embryos
Stain results:
| Bone: | Red | |
| Soft Tissue: . | Transparent and unstained |
References:
- Cumley, R.W., Crow, J.F., and Griffin, A.B., Stain Techn., 14:7-11, 1939
- Dawson, A.B., Stain Techn., 1:123-124, 1926
- Richmond, G.W. and Bennett, L., Stain Techn., 13:77-79, 1938
- Clark, G., (ed), Staining Procedures, 3rd ed., William & Wilkins, Baltimore, p. 138, c. 1973
| RT | 26204-01 | Alizarin S, 0.01% Aqueous | |
500 ml | 22.00 | Add to Cart |
| RT | 26204-02 | Potassium Hydroxide, 1% Aqueous | 500 ml | 21.00 | Add to Cart | |
| RT | 26204-03 | Potassium Hydroxide, 10% Aqueous | 500 ml | 22.00 | Add to Cart | |
| RT | 26204-04 | Glycerol-Potassium Hydroxide | 500 ml | 22.00 | Add to Cart |
Alizarin Red S and Toluidine Blue O
Williams (1941); Dawson (1926)
Distinction between bone and cartilage in mammalian embryos.
Stain results:
| Soft Tissues: | Transparent | |
| Osseous Tissues: | Deep Red | |
| Cartilage: | Dark Blue |
References:
- Dawson A.B., Stain Techn., 1:123-4, 1926
- Williams, T.W., Stain Techn., 16:23-5, 1941
- Clark, G., (ed), Staining Procedures, 3rd Ed., Williams & Wilkins Baltimore, p. 139, c. 1973
| RT | 26205-01 | Alizarin Red S, 0.02% Aqueous | |
500 ml | 22.00 | Add to Cart |
| RT | 26205-1A | Potassium Hydroxide, 4%, Aqueous | 500 ml | 23.00 | Add to Cart | |
| RT | 26205-02 | Toluidine Blue O, 0.25% Aqueous | |
500 ml | 35.00 | Add to Cart |
| RT | 26205-03 | Ammonium Alcohol | 500 ml | 24.00 | Add to Cart | |
| RT | 26205-05 | Potassium Hydroxide, 2%, Aqueous | |
500 ml | 24.00 | Add to Cart |
| RT | 26205-06 | Sulfuric Acid Alcohol | |
500 ml | 20.00 | Add to Cart |
Alizarin Red S for Calcium Deposits
Calcium deposits stain.
Stain Results:
| Calcium Sites: | Covered and surrounded by a heavy orange red precipitate which is birefringent between crossed polaroids |
|
| Background: | Faint Pink |
References:
- McGee-Russell, S.M., J. Histochem., 6:22-42, 1958.
- Clark, G., (ed), Staining Procedures, 3rd d., Williams & Wilkins, Baltimore, p. 140, c. 1973.
| RT | 26206-01 | Alizarin S, 2% Solution, pH 4.2 | |
250 ml | 17.00 | Add to Cart |
| RT | 26206-02 | Reagent Alcohol, 50% | 500 ml | 15.00 | Add to Cart | |
| RT | 26206-03 | Acetone | |
1 qt | 14.00 | Add to Cart |
| RT | 26206-04 | Acetone-Xylene | 500 ml | 22.00 | Add to Cart |
Pizzolato's Method for Calcium Oxalate
For bone and its related stain.
Stain results:
| Calcium oxalate: | Black | |
| All other tissue elements: | Red |
References:
- Pizzolato, P., J. Histochem. Cytochem., 12:333-136, 1964.
- Luna, L.G., (ed), Manual of Staining Methods of the AFIP, 3rd Ed, McGraw-Hill, NY, c 1968, p 77.
| RT | 26212-01 | Silver Nitrate, 5% Aqueous | |
250 ml | 83.00 | Add to Cart |
| RT | 26212-02 | Hydrogen Peroxide, 30% | |
450 ml | 22.00 | Add to Cart |
| RT | 26212-03 | Nuclear Fast Red (Kernechtrot) Solution | |
500 ml | 43.00 | Add to Cart |
D. BONE MARROW STAINS
May-Grunwald / Giemsa Combined Stain
Beck (1938); Pappanheim (1912)
Stain results:
| Nuclei, Bacteria | Blue | |
| Cytoplasm | Pink-Rose |
References
- Beck, R.C., Laboratory Manual of Hematological Technique, W.B. Saunders & Co., Philadelphia, c. 1938, pp 233-236
- Clark, G. (ed), Staining Procedures, 3rd ed. Williams &Wilkins, Baltimore, c. 1973, pp 131-132
- Strumia, M.M., J Lab Clin. Med 21:930-934, 1935-1936
- Luna, L.G., (ed) Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd ed. McGraw-Hill, NY, c. 1968, pp 121-122
| RT | 26250-01 | May-Grunwald Staining Solution OR |
|
500 ml | 32.00 | Add to Cart |
| RT | 26250-1A | Jenner Staining Solution | 500 ml | 36.00 | Add to Cart | |
| RT | 26250-02 | Giemsa Staining Solution | |
250 ml | 32.00 | Add to Cart |
Maximow's Method for Bone Marrow
Mallory (1961)
Morphology details of marrow cells.
Stain results:
| Nuclei | Blue | |
| Basophile leukocyte, mast cell granules | Purple to Violet | |
| Cartilage | Purple | |
| Erythrocytes, eosinophile granules | Pink | |
| Cytoplasm | Blue to Pink |
References
- AFIP Manual of Histologic Staining Methods, 3rd Ed, Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 120
- Mallory, F.B., Pathological technique, New York, Hafner Publishing Co., 1961, p.196
| RT | 26252-01 | Mayer's Hematoxylin | |
500 ml | 44.00 | Add to Cart |
| RT | 26252-02 | Eosin Y, 0.1%, Aqueous | 500 ml | 31.00 | Add to Cart | |
| RT | 26252-03 | Azur II, 0.1%, Aqueous | 500 ml | 38.00 | Add to Cart | |
| RT | 26252-04 | Phosphate Buffer, 0.1M, pH 6.8-7.0 | |
250 ml | 19.00 | Add to Cart |
