Prepared Staining Kits for Histology and Pathology
E. CARBOHYDRATE & MUCOSUBSTANCE STAINS:

Crystal Violet Stain for Amyloid
Stain results:
Amyloid: |
|
Violet-Red |
Tissue: |
|
Blue |
References:
- Clark, G.: Staining Procedures, Williams and Wilkin co., Baltimore 3rd Ed., c. 1973, p.48.
- Conn, H. J., Biological Stains 4th Ed. Biotech Publications, Geneva New York, 1940.
RT |
26301-01 |
Crystal Violet Solution |
 |
500 ml |
51.00 |
Add to Cart |
RT |
26301-02 |
Acetic Acid, 1% Aqueous |
 |
500 ml |
17.00 |
Add to Cart |

Sweat-Puchtler Method for Amyloid
Stain results:
Amyloid: |
|
Pink-Red |
Nuclei: |
|
Blue |
Elasticia: |
|
Pink to Red |
Background: |
|
Unstained |
References:
- Sweat, F. and Puchtler, H.: Arch. Path. 80:613, 1965
- AFIP Manual of Histologic Staining Methods, 3rd edition, Ed L. Luna: New York: McGraw Hill Publication, c. 1968, p. 154
RT |
26303-01 |
Sirius Red F3BA Solution, 1% |
 |
500 ml |
43.00 |
Add to Cart |
RT |
26303-02 |
Mayer Hematoxylin |
 |
500 ml |
43.00 |
Add to Cart |
RT |
26303-03 |
Borate Buffer, 0.1M – pH 9.0 |
 |
500 ml |
21.00 |
Add to Cart |
RT |
26303-04 |
Alkaline Alcohol |
 |
500 ml |
24.00 |
Add to Cart |

Laqueur's Method for Alcoholic Hyalin Lacqueur (1950)
For mallory bodies, erythrocytes, bile pigment and proteinaceous
material in liver.
Stain Results:
Mallory Bodies: |
|
Bright Red |
Erythrocytes: |
|
Red |
Cytoplasm: |
|
Pale Brown |
Bile Pigment: |
|
Green |
Proteinaceous material seen occasionally in some liver cells: |
|
Red |
Hemosiderin and lipofichsin: |
|
Unstained |
References:
- Laqueur, G.I., Amer. J. Clin. Path., 20:689-690, 1950
- Luna, L.G. (ed), Manual of Hitopathologic Staining Method of the AFIP, 3rd ed, McGraw-Hill, NY, c. 1968, p 160
- Lillie, R.D., Fullmer, H.M., Histopathologic Technic and Practical Histochemistry 4th ed., cGraw-Hill, NY, 1976, p 670-671
- Clark, G., (ed) Staining Procedures, 3rd ed. WilliamS & Wilkins, Baltimore, p 51, c. 1973
RT |
26306-01 |
Mayer's Hematoxylin |
 |
500 ml |
40.00 |
Add to Cart |
RT |
26306-02 |
Acid Fuchsin-Aniline Solution |
|
500 ml |
66.00 |
Add to Cart |
RT |
26306-03 |
Light Green, 1% Aqueous |
|
500 ml |
38.00 |
Add to Cart |
RT |
26306-04 |
Phosphomolybdic Acid, 1% Aqueous |
 |
500 ml |
36.00 |
Add to Cart |
RT |
26306-05 |
Alcoholic Picric Acid |
 |
500 ml |
32.00 |
Add to Cart |
E.2 Glycogen Stains

McManus’ Method (PAS) for Glycogen
McManus (1948)
Stain results:
Nuclei: |
|
Blue |
Fungi: |
|
Red |
Background, when Light Green is used
as the counter-stain: |
|
Green, pale |
References:
- McManus, JFA: Stain Tech.; 23:99 (1948)
- AFIP Manual of Histologic Staining Techniques; L.G. Luna, 3rd Ed., New York: McGraw-Hill Publications, c. 1968, p. 160.
- Mowry, RW: Annals of the New York Academy of Science, 106:402 (1963).
RT |
26312-01 |
Coleman's Feulgen
OR |
 |
500 ml |
46.00 |
Add to Cart |
RT |
26312-1A |
Schiff's Reagent |
 |
500 ml |
48.00 |
Add to Cart |
RT |
26312-02 |
Light Green Stock Solution, 0.2% OR |
 |
250 ml |
18.00 |
Add to Cart |
RT |
26312-2A |
Light Green Working Solution OR |
 |
500 ml |
34.00 |
Add to Cart |
RT |
26312-2B |
Harris Hematoxylin |
 |
500 ml |
34.00 |
Add to Cart |
RT |
26312-03 |
Periodic Acid, 0.5%, Aqueous |
 |
500 ml |
25.00 |
Add to Cart |
RT |
26312-04 |
Acid Alcohol, 1% |
 |
500 ml |
20.00 |
Add to Cart |
RT |
26312-05 |
Ammonia Water, 0.3% |
 |
500 ml |
24.00 |
Add to Cart |
E.3 Mucosubstance Stains

Mayer Mucicarmine Method
For mucosubstances stains.
Stain results:
Mucin, Cryptococci Capsulae: |
|
Deep Rose to Red |
Other Tissue Elements: |
|
Yellow |
Nuclei: |
|
Black |
Note: The rose color due to carmin staining will be obscured if sections are overstained with Weigert's Hematoxylin and/or Metanil Yellow solution
References:
- Mallory, F.B.: Pathological Technique. Hafner Publishing Co., N.Y, c. 1961, p. 130.
- Luna, L.G., (ed), AFIP Manual of Histologic Staining Methods, 3rd. ed., McGraw-Hill Co., NY, C. 1968, pp161-162
- Sheehan, D.C. & Hrapenak, B.B., Theory and Practice of Histotechnology, 2nd ed., C.V. Mosby Co., St. Louis. C. 1980. pp 168-169
RT |
26320-01 |
Mucicarmine Stock Carmine Solution |
 |
500 ml |
64.00 |
Add to Cart |
RT |
26320-02 |
Metanil Yellow, 0.25% |
 |
500 ml |
26.00 |
Add to Cart |
RT |
26320-03 |
Weigert's Hematoxylin Solution A |
 |
500 ml |
36.00 |
Add to Cart |
RT |
26320-04 |
Weigert's Hematoxylin Solution B |
 |
500 ml |
27.00 |
Add to Cart |

Modification of Mayer's Mucihematein
Lillie (1965); Laskey (1950)
For stain mucins, especially those derived from epithelial cells of glandular tissue.
Stain results:
Mucin: |
|
Deep Violet |
Cell Nuclei: |
|
Pale Gray Blue |
Connective Tissue: |
|
Pale Gray to colorless |
References:
- Laskey, Alice: Stain Technology, 25:33 (1959)
- Clark G.: Staining Procedures, Williams & Wilkins Co., Baltimore, 3rd Ed., c. 1973, p. 57.
RT |
26321-01 |
Mayer's Mucihematein, Modified |
 |
500 ml |
52.00 |
Add to Cart |

Alcian Blue Method (pH 2.5)
For stain weakly acidic sulfated mucosubstances, hyaluronic acid and
sialomucins.
Stain results:
Weakly Acidic Sulfated Mucosubstances: |
|
Dark Blue |
Hyaluronic Acid: |
|
Dark blue |
Sialomucins: |
|
Dark blue |
References:
- AFIP Manual of Histological Stains Methods, 3rd Ed, Ed. L. Luna: New York McGraw Hill Publications, c. 1968, p. 163.
- Lev, R. & Spicer. S.S., J. Histochem Cystochem, 12:309, 1964
RT |
26323-01 |
Alcian Blue, 1% - pH 2.5 |
 |
250 ml |
46.00 |
Add to Cart |
RT |
26323-02 |
Nuclear Fast Red (Kernechtrot) Solution |
 |
250 ml |
26.00 |
Add to Cart |
RT |
26323-03 |
Acetic Acid, 3% Aqueous |
 |
500 ml |
18.00 |
Add to Cart |

Aldehyde-Fuchsin Method (pH 1.0)
For stain highly acid sulfated mucosubstances.
Stain results:
All mucins except the highly acidic sulfated mucosubstances are
aldehyde fuchsin negative at pH values of 1.0 and below.
References:
- Johnson, W.C., Graham, J.H., and Helwig, E.B., J. Invest. Derm., 42:215-224, 1964
- Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd Ed., McGraw-Hill, c.1968, p. 166
RT |
26328-01 |
Aldehyde-Fuchsin Solution – pH 1.0 |
 |
500 ml |
43.00 |
Add to Cart |
RT |
26328-02 |
Metanil Yellow Solution, 0.25% |
 |
500 ml |
29.00 |
Add to Cart |
RT |
26328-03 |
Alcohol Solution, 80% – pH 1.0 |
 |
500 ml |
28.00 |
Add to Cart |