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Electron Microscopy Sciences

Histology and Light Microscopy

arrow13Prepared Staining Kits for Histology and Pathology

E. CARBOHYDRATE & MUCOSUBSTANCE STAINS:

Alcoholic Stain SolutionsarrowCrystal Violet Stain for Amyloid

Stain results:

Amyloid:   Violet-Red
Tissue:   Blue

References:

  • Clark, G.: Staining Procedures, Williams and Wilkin co., Baltimore 3rd Ed., c. 1973, p.48.
  • Conn, H. J., Biological Stains 4th Ed. Biotech Publications, Geneva New York, 1940.
RT 26301-01 Crystal Violet Solution msds 500 ml 50.00 Add to Cart
RT 26301-02 Acetic Acid, 1% Aqueous msds 500 ml 16.00 Add to Cart

Alcoholic Stain SolutionsarrowSweat-Puchtler Method for Amyloid

Stain results:

Amyloid:   Pink-Red
Nuclei:   Blue
Elasticia:   Pink to Red
Background:   Unstained

References:

  • Sweat, F. and Puchtler, H.: Arch. Path. 80:613, 1965
  • AFIP Manual of Histologic Staining Methods, 3rd edition, Ed L. Luna: New York: McGraw Hill Publication, c. 1968, p. 154
RT 26303-01 Sirius Red F3BA Solution, 1% msds 500 ml 42.00 Add to Cart
RT 26303-02 Mayer Hematoxylin   500 ml 42.00 Add to Cart
RT 26303-03 Borate Buffer, 0.1M – pH 9.0 msds 500 ml 20.00 Add to Cart
RT 26303-04 Alkaline Alcohol5   500 ml 23.00 Add to Cart

Alcoholic Stain SolutionsarrowLaqueur's Method for Alcoholic Hyalin Lacqueur (1950)

For mallory bodies, erythrocytes, bile pigment and proteinaceous
material in liver.

Stain Results:

Mallory Bodies:   Bright Red
Erythrocytes:   Red
Cytoplasm:   Pale Brown
Bile Pigment:   Green
Proteinaceous material seen occasionally in some liver cells:   Red
Hemosiderin and lipofichsin:   Unstained

References:

  • Laqueur, G.I., Amer. J. Clin. Path., 20:689-690, 1950
  • Luna, L.G. (ed), Manual of Hitopathologic Staining Method of the AFIP, 3rd ed, McGraw-Hill, NY, c. 1968, p 160
  • Lillie, R.D., Fullmer, H.M., Histopathologic Technic and Practical Histochemistry 4th ed., cGraw-Hill, NY, 1976, p 670-671
  • Clark, G., (ed) Staining Procedures, 3rd ed. WilliamS & Wilkins, Baltimore, p 51, c. 1973
RT 26306-01 Mayer’s Hematoxylin msds 500 ml 40.00 Add to Cart
RT 26306-02 Acid Fuchsin-Aniline Solution   500 ml 66.00 Add to Cart
RT 26306-03 Light Green, 1% Aqueous   500 ml 38.00 Add to Cart
RT 26306-04 Phosphomolybdic Acid, 1% Aqueous msds 500 ml 33.00 Add to Cart
RT 26306-05 Alcoholic Picric Acid   500 ml 31.00 Add to Cart

E.2 Glycogen Stains

Alcoholic Stain SolutionsarrowMcManus’ Method (PAS) for Glycogen

McManus (1948)

Stain results:

Nuclei:   Blue
Fungi:   Red
Background, when Light Green is used
as the counter-stain:
  Green, pale

References:

  • McManus, JFA: Stain Tech.; 23:99 (1948)
  • AFIP Manual of Histologic Staining Techniques; L.G. Luna, 3rd Ed., New York: McGraw-Hill Publications, c. 1968, p. 160.
  • Mowry, RW: Annals of the New York Academy of Science, 106:402 (1963).
RT 26312-01 Coleman's Feulgen
OR
msds 500 ml 45.00 Add to Cart
RT 26312-1A Schiff's Reagent msds 500 ml 46.00 Add to Cart
RT 26312-02 Light Green Stock Solution, 0.2% l
OR
msds 250 ml 17.00 Add to Cart
RT 26312-2A Light Green Working Solution
OR
msds 500 ml 32.00 Add to Cart
RT 26312-2B Harris Hematoxylin msds 500 ml 32.00 Add to Cart
RT 26312-03 Periodic Acid, 0.5%, Aqueous msds 500 ml 24.00 Add to Cart
RT 26312-04 Acid Alcohol, 1% msds 500 ml 19.00 Add to Cart
RT 26312-05 Ammonia Water, 0.3%   500 ml 23.00 Add to Cart

E.3 Mucosubstance Stains

Alcoholic Stain SolutionsarrowMayer Mucicarmine Method

For mucosubstances stains.

Stain results:

Mucin, Cryptococci Capsulae:   Deep Rose to Red
Other Tissue Elements:   Yellow
Nuclei:   Black

Note: The rose color due to carmin staining will be obscured if sections
are overstained with Weigert's Hematoxylin and/or Metanil Yellow
solution

References:

  • Mallory, F.B.: Pathological Technique. Hafner Publishing Co., N.Y, c. 1961, p. 130.
  • Luna, L.G., (ed), AFIP Manual of Histologic Staining Methods, 3rd. ed., McGraw-Hill Co., NY, C. 1968, pp161-162
  • Sheehan, D.C. & Hrapenak, B.B., Theory and Practice of Histotechnology, 2nd ed., C.V. Mosby Co., St. Louis. C. 1980. pp 168-169
RT 26320-01 Mucicarmine Stock Solution   500 ml 64.00 Add to Cart
RT 26320-02 Metanil Yellow, 0.25% msds 500 ml 26.00 Add to Cart
RT 26320-03 Weigert's Hematoxylin Solution A msds 500 ml 36.00 Add to Cart
RT 26320-04 Weigert's Hematoxylin Solution B msds 500 ml 27.00 Add to Cart

Alcoholic Stain SolutionsarrowModification of Mayer’s Mucihematein

Lillie (1965); Laskey (1950)

For stain mucins, especially those derived from epithelial cells of
glandular tissue.

Stain results:

Mucin:   Deep Violet
Cell Nuclei:   Pale Gray Blue
Connective Tissue:   Pale Gray to colorless

References:

  • Laskey, Alice: Stain Technology, 25:33 (1959)
  • Clark G.: Staining Procedures, Williams & Wilkins Co., Baltimore, 3rd Ed., c. 1973, p. 57.
RT 26321-01 Mayer’s Mucihematein, Modified 500 ml 52.00 Add to Cart

Alcoholic Stain SolutionsarrowAlcian Blue Method (pH 2.5)

For stain weakly acidic sulfated mucosubstances, hyaluronic acid and
sialomucins.

Stain results:

Weakly Acidic Sulfated Mucosubstances:   Dark Blue
Hyaluronic Acid:   Dark blue
Sialomucins:   Dark blue

References:

  • AFIP Manual of Histological Stains Methods, 3rd Ed, Ed. L. Luna: New York McGraw Hill Publications, c. 1968, p. 163.
  • Lev, R. & Spicer. S.S., J. Histochem Cystochem, 12:309, 1964
RT 26323-01 Alcian Blue, 1% - pH 2.5 msds 250 ml 42.00 Add to Cart
RT 26323-02 Nuclear Fast Red (Kernechtrot) Solution msds 250 ml 24.00 Add to Cart
RT 26323-03 Acetic Acid, 3% Aqueous msds 500 ml 17.00 Add to Cart

Alcoholic Stain SolutionsarrowAldehyde-Fuchsin Method (pH 1.0)

For stain highly acid sulfated mucosubstances.

Stain results:

All mucins except the highly acidic sulfated mucosubstances are
aldehyde fuchsin negative at pH values of 1.0 and below.

References:

  • Johnson, W.C., Graham, J.H., and Helwig, E.B., J. Invest. Derm., 42:215-224, 1964
  • Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd Ed., McGraw-Hill, c.1968, p. 166
RT 26328-01 Aldehyde-Fuchsin Solution – pH 1.0 msds 500 ml 39.00 Add to Cart
RT 26328-02 Metanil Yellow Solution, 0.25% msds 500 ml 28.00 Add to Cart
RT 26328-03 Alcohol Solution, 80% – pH 1.0 msds 500 ml 27.00 Add to Cart

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