Certified Dyes/Stains
Acid
Fuchsin, Certified, C.N. #DcR-8
(Rubin S, Fuchsin S, Fuchsin Acid, Acid Magenta, Acid Violet 19) C20H17N3Na2O9S3 F.W. 585.54 CAS #3244-88-0 Solubility: 0.053% Water; 1.21% Alcohol
| RT | 10035 | 25 g | 30.00 | Add to Cart |
Acridine
Orange
(Basic Orange 14) C17H2ON3 Cl F.W. 301.82 CAS #65-61-2
Ultrastructural probes on DNA templates within human bone marrow and lymph node cells. Frenstar, J., Adv. Cell Mol. Biol., 3, 1(1974). Used to detect changes in DNA template activity within cells. Frenster, J. Cell Nucl., 1, 565 (1974) Fluorescent dye for cytochemistry. J. Histochem. Cytochem., 31, 737 ((1983) J. Lab. Med., 15 (3),180, (1984)
| RT | 10050 | 25g | 62.00 | Add to Cart |
Alcian
Blue 8GX, C.N. #AnAn-6 (Ingrain Blue I)
C56H68N16S4Cu F.W. 1298.88 CAS #12040-44-7 Dye Content: Approx. 50% Solubility: Water-soluble
Used as a fixative: 0.5-2% Alcian Blue in Glutaraldehyde fixative enhances the preservation and constrast of the cell coat and intercellular substances. Used as a stain: For staining cell surface materials and cartilage components. Schofield, et al., Histochem J. 7, 139,1975 Used as a wetting agent: Sommer, J. R (1977). To cationize glass. J.Cell Bio. 75, 245a. Alcian Blue and Ruthenium Red were used to study cartilage ultrastructure. Thyberg, J., Lohmander, S., and Friberg, U., J. Ultrastruct. Res., 45, 407 (1973).
| RT | 10350 | 25g | 190.00 | Add to Cart |
Technical Tip
Alizarin
Red S, Certified, C.N. #DcAr-5
(Alizarin Camine, Mordant Red 3) C14H7O7SNa.H2O F.W. 360.28 CAS #130-22-3 Solubility: 7.69% Water; 0.1% Alcohol.
Certified for Staining Bone. Stain for nervous tissue in small invertebrates, and for sections of nervous tissue. Lee, "The Microtomist's Vade-Mecum", 10th Ed., The Blakiston Co., Phila., 1937; Dawson, Stain Tech., 1, 123 (1926); Lundvall, Anat. Anz., 27, 521 (1905); 137. Use to differentiate bone from cartilage in mammalian embryos; used with Toluidine Blue. William, Stain Tech., 16, 23 (1941)
| RT | 10360 | 25g | 39.00 | Add to Cart |
Aniline
Blue Disodium Salt, Water Soluble, Certified, C.N. #DcK-11
(Acid Blue 22; China Blue; Cotton Blue; Marine Blue V; Soluble Blue 3M, 2R; Water Blue) C32H25N3O9S3Na2 F.W. 737.74 CAS #28631-66-5
Solubility: 50% Water; 0.0% Alcohol; 4.5% Cellosolve; 7.8% Glycol; 0.0% Xylene Used in connective tissue staining procedures to stain collagen. Used in differential staining. Snodgress el al., Lab Invest. 26:329, 1972.
| RT | 10840 | 25g | 32.00 | Add to Cart |
Auramine
O, Certified, C.N. # DcAu-4
(Pyoctanunum aureum; Basic Yellow 2; Pyoktanin Yellow; Canary Yellow) C17H21N3HCl·H2O F.W. 321.86 CAS # 2465-27-2 Solubility: 1.0% Water; 4.0% Alcohol; 1.2% Cellosolve; 1.75% Glycol; 0.05% Xylene
| RT | 10850 | 25 g | 78.00 | Add to Cart |
Azocarmine
G
(Acid red 101; Basalan Carmine GE; Rosinduline) C28H17N3Na2O6S2 F.W. 601.57 CAS #25641-18-3 Solubility: 1.0% Water; 0.1% Alcohol; 1.75% Cellosolve; 4.5% Glycol; 0.01% Xylene
| RT | 11150 | 5 g | 37.00 | Add to Cart |
Azur
A (Methylene Azur A)
C14H14ClN3S F.W. 291.80 CAS #531-53-3 Solubility: Water-soluble; Alcohol-soluble Used in nuclear staining; A polychromatic blood stain.
| RT | 11240 | 10g | 80.00 | Add to Cart |
Azur
B (Azur I; Methylene Azur B)
C15H16N3SBr FW 350.29 CAS #531-55-5
Solubility: Water-soluble; Alcohol-slightly soluble. An important component of Giemsa's stain for blood protozoa. Used for staining semi-thin sections of plant tissue. Hoefert, L.L. (1968). Polychromatic stains for thin sections of Beta embedded in epoxy resin. Stain Tech. 43:145
| RT | 11200 | 10g | 52.00 | Add to Cart |
Azur
II
(Azur B/Methylene Blue 1:1) CAS #37247-10-2
| 11210 | 10g | 38.00 | Add to Cart |
Azur
II Eosin
(Azur B/Methylene Blue/Eosin 1:1:1) CAS #53092-85-6
| RT | 11220 | 10g | 56.00 | Add to Cart |
Basic
Fuchsin, Certified, C.N. #DcFb-26
(Rasaniline Chloride; Magenta; Basic Violet 14) C20H19N3.HCl F.W. 337.86 CAS #632-99-5
Berkowitz et al., (1968). Selective staining of nervous tissue for light microscopy following preparation for electron microscopy. J. Histochem. Cytochem. 16:808. Sato, T., and Shamoto, M (1973). A simple rapid polychrome stain for epoxy-embedded tissue. Stain Technol. 48:223.
| RT | 11260 | 25g | 80.00 | Add to Cart |
Basic
Green 4, Certified, C.N. #DcMg-11
(Malachite Green Oxalate; Diamond Green B; BX or P Extra; Light Green N, Solid Green O; Victoria Green B or WB) 2(C23H25N2).2(C2H2O4).C2O4 F.W. 927.03 CAS #2437-29-8 Solubility: 10.0% Water; 8.5% Alcohol; 5.5% Cellosolve; 7.0% Glycol; 0.0% Xylene. Used as a bacteria stain and a counterstain for botanical material.
| RT | 18100 | 25g | 42.00 | Add to Cart |
Biebrich
Scarlet, Water Soluble
(Bonceau BS; Acid Red 66; Croceine Scarlet; Double Scarlet BSF) C22H14N4Na2O7S2 F.W. 556.4 9 CAS #4196-99-0 Solubility: 5.0% Water; 0.25% Alcohol; 1.5% Cellosolve; 1.5% Glycol; 0.0% Xylene
| RT | 11265 | 25g | 28.00 | Add to Cart |
Bismarck
Brown Y
(Basic Brown 1; Basic Brown G, GX, GXP; Vesuvine; Leather Brown; Manchester Brown; Phenylene Brown) C18H18N8·2HCl F.W. 419.32 CAS #10114-58-6 Solubility: 1.5% Water; 3.0% Alcohol; 3.0% Cellosolve; 7.9% Glycol; 0.05% Xylene
| RT | 11310 | 25g | 20.00 | Add to Cart |
Brilliant
Cresyl Blue, Certified, C.N.#DcV-5
(Brilliant Blue C; Cresyl Blue 2RN) C17H21N4OCl F.W. 332.84 CAS #10127-36-3 Solubility: 3.0% Water; 2.6% Alcohol; 2.25% Cellosolve; 8.0% Glycol; 9.5% Xylene
| RT | 11465 | 10g | 52.00 | Add to Cart |
Bromophenol
Blue, Reagent, A.C.S.
(3',3",5 '5"-Tetrabromophenol-sulfonthalein) C19H10O5SBr4 F.W. 669.97 CAS #115-39-9
| 11470 | 5g | 25.00 | Add to Cart | ||
| 11471 | 25g | 88.00 | Add to Cart |
Carmine,
Certified, C.N.#DcCa-16
Alum Lake of Carminic acid
(Natural Red 4)
CAS # 1390-65-4
Solubility: 0.0% Water; 0.2% Alcohol; 0.5% Cellosolve; 4.0% Glycol; 0.0% Xylene.
| RT | 12410 | 10 g | 29.00 | Add to Cart |
Cresyl
Fast Violet, Certified, C.N.# DcW-5
(Cresyl Violet Acetate)
C18H15N3O3 F.W. 312.34 CAS
# 10510-54-0
Solubility: 0.13% water
| RT | 12780 | Cresyl Fast Violet, Certified, | 5 g | 78.00 | Add to Cart |
Crystal
Violet, Certified, C.N. #DcC-38
(Basic Violet 3; Gentian Violet; Methyl Violet 10B; Hexamethyl Violet)
C25H30N3Cl F.W. 407.99 CAS #548-62-9
Solubility: 9.0% Water; 8.75% Alcohol; 7.5% Cellosolve; 7.0% Glycol; 0.0% Xylene
| RT | 12785 | Crystal Violet | 25 g | 22.00 | Add to Cart |
Eosin
Y, Certified, C.N. #DcE-40
(Acid Red 87, Eosin Yellowish)
C2cH6Br4Na2O5 F.W.
691.88 CAS #17372-87-1
Solubility: 44.0% Water; 2.0% Alcohol; 25.0% Cellosolve; 27.5% Glycol;
0.0% Xylene.
Used with Hematoxylin for showing cell nuclei in embryos and kidneys. Conn, "Biological
Stain" 9th ed., The Williams and Wilkens Co., Baltimore 1977, pg. 7.
Component of Wright's stain for blood corpuscles. Wright, J. Med.
Res., 7, 138 (1902)
For staining bone marrow to show cell morphology. Bech, "Lab. Manual of
Hematological Tech.", W. B. Saunders Co., Phila., 1908, p. 233
| RT | 14850 | 10g | 10.00 | Add to Cart | |
| RT | 14851 | 25g | 20.00 | Add to Cart |
Erythrosin
B, Certified, C.N. #DcEr-5
(Acid Red 51, Erythrosin extra Bluish; Eosin J; Iodeosin B; Pyrosin
B)
C20H6O5I4Na2 F.W.
879.87 CAS #16423-68-0
Solubility: 10.0% Water; 5.0% Alcohol; 7.0% Cellosolve; 8.25% Glycol; 0.0%
Xylene.
| RT | 15010 | 10 g | 23.00 | Add to Cart |
Fast
Green FCF, Certified, C.N. #DcGf-12
(Food Green 3)
C37H34O10N2S3Na2 F.W.808.86 CAS
#2353-45-9
Solubility:4.0% Water; 9.0% Alcohol; 8.0% Cellosolve; 6.25% Glycol; 0.0% Xylene
The main advantage of this dye is its resistance to fading. Mammalian tissue stain for visualizing collagen, muscle, cystoplasma, cells and cornified epithelium. Kornhauser, Stain Tech., 18,95 (1943); 20, 23 (1945); Lillie, Arch. Path., 29, 705 (1940). Modified Feulgen stain for visualizing chromosomes in root tips and other meristematic tissue. DeTomasi, Stain Tech., 11, 137 (1936). Used in plant histology. O. Conn, "Biological Stains" 9th ed., Williams & Wilkens Co., Baltimore, 1977, pp. 252-3; Haynes, Stain Tech., 3, 40 (1928).
| RT | 15500 | 5g | 22.00 | Add to Cart |
Giemsa Solution, C.N.#DcGe-19
| Glycerin (CAS#: 56-81-5): | 61% |
| Methyl Alcohol (CAS#: 67-56-1): | 38.3% |
| Azur II (CAS#: 37247-10-2): | 0.16% |
| Azur II Eosin (CAS#: 53092-85-6): | 0.06% |
Specific Gravity: 0.89
Differentiates types of leucocytes, bacteria and inclusion bodies.
Giemsa, Centralbl. F. Publ., Abt. 1,37, 308 (1904) Stain for thick blood films;
shows malarial parasites. Barber and Komp, Publ. Health Rep., 44, 2330 (1929)
Bone marrow and stain for showing cell morphology; together with May-Grunwald
Solution for pap staining. Pappenheim, Folia Haemat., 13, 338 (1912)
| RT | 15940 | Giemsa Stain Solution | 100 ml | 20.00 | Add to Cart |
Giemsa Stain, C.N.#DcGe-19, Powder
CAS #51811-82-6
See the listing in Certified
Dyes/Stains
| RT | 15942 | 10g | 40.00 | Add to Cart |
Hematoxylin, Certified, C.N.#DcH-48
(Natural Black I, Hydroxybrasilin)
C16H14O6 F.W. 302.29 CAS
#517-28-2
Solubility: 10.0% Water; 10.0% Alcohol; 9.5% Cellosolve; 10%
Glycol; 0.0% Xylene. A certified biological stain used in histology
and cytology and plastic embedded tissues.
Chen, Chang, S., J Arch. of Path., 93,334 (1972).
| RT | 16620 | 10g | 28.00 | Add to Cart |
Technical Tip
Indigo Carmine, Certified, C.N.
#DcI-7
(Acid Blue 74; Carmine Blue; Indigotine 1, 1A)
C16H8N2O8S2Na2 F.W.466.36 CAS
#860-22-0
Dye content: approx. 85%
Solubility: 1.3% Water; 0.0% Alcohol; 4.0% Cellosolve; 2.85% Glycol; 0.0%
Xylene
Shunway's stain for animal embryos. Used with Acid Fuchsin for staining Negri
bodies. Stain Tech., 5, 34 (1928)
Counterstain to Hematoxylin for staining vaginal smears. Am. J. Anat., 61,
505 (1937).
| RT | 16910 | 25g | 17.00 | Add to Cart |
Technical Tip
When a stain is applied both en-bloc and as a post-stain for thin sections, maximum contrast will be achieved.
Indium Trichloride,
Anhydrous
InCl3 F.W. 221.18 CAS
#10025-82-8
Chloride Content: 98%
A metal stain for nucleic acid
J. Biophys Biochem Cytol, 11,257 (1961)
| RT | 16920 | 5g | 60.00 | Add to Cart |
Light Green
SF Yellowish, Certified, C.N. #DcL-30
(Acid Green 5; Light Green 2G, S or 2GN;
Acid Green F; Fast Acid Green N)
C37H34N2Na2O9S3 F.W.
792.86 CAS #5141-20-8
Solubility: 20.35% Water; 4.0% Alcohol; 5.25% Cellosolve; 12.0% Glycol; 0.0%
Xylene
| RT | 17920 | 10 g | 15.00 | Add to Cart |
Malachite
Green 4, Certified, C.N. #DcMg-11
(Malachite Green Oxalate; Diamond Green B; BX or P Extra; Light Green N, Solid Green O; Victoria Green B or WB) 2(C23H25N2).2(C2H2O4).C2O4 F.W. 927.03 CAS #2437-29-8 Solubility: 10.0% Water; 8.5% Alcohol; 5.5% Cellosolve; 7.0% Glycol; 0.0% Xylene. Used as a bacteria stain and a counterstain for botanical material.
| RT | 18100 | 25g | 42.00 | Add to Cart |
Methylene
Blue Chloride, Certified, C.N. #DcA-24
(Basic Blue 9; Swiss Blue)
C16H18N3SCl •3H2O F.W. 319.86+aq CAS
#7220-79-3
Solubility: 9.5% Water; 6.0% Alcohol; 6.5% Cellosolve; 10.0% Glycol; 0.0%
Xylene.
- Used to distinguish autonomic nerves. Richardson, K.C., (1969) Anat. Rec., 164, 231. General stain for animals, histology.
- A simple rapid staining procedure for methacrylate embedded tissue sections using chromotrope 2R and Methylene Blue. Dougherty, M.M., and King, J.S., (1984) Stain Tech., 59, 149
- An established routine method for differential staining of epoxy-embedded tissue sections. Schroeder, H.E., Rossinsky, K., and Muller, W., (1980) Microsc. Acta 83, 111
- Used with Basic Fuchsin for stain epoxy-embedded tissue. Sato, T. and Shamoto, M. (1973). A simple rapid polychrome stain for epoxy-embedded tissue. Stain Technol., 48:223
| RT | 18600 | 25g | 38.00 | Add to Cart |
Methyl Green,
Certified, C.N. #DcG-10
(Zinc Chloride Salt; Ethyl Green)
C27H35N3C12·ZnC12 F.W.
608.79 CAS #7114-03-6
Solubility: 8.0% water; 3.0% Alcohol; 1.8% Cellosolve; 3.5% Glycol; 0.0%
Xylene.
- Used as a counterstain in Almann's aniline acid fuchsin technique for mitochrondria; used in Kurnick's Methyl Green-Pyonin stain for nuclei and cystoplasmic granules of liver cells. Marker for DNA at acid and neutral conditions. J.B. Boyd, H.R. Mitchell, (1965) Anal. Biochem., 13, 28.
- Used for epoxy-embedded thick sections. Sievers, J. (1971).
- Basic two-dye stains for epoxy-embedded 0.3-1 micron sections. Stain Technol., 46:195
| RT | 18700 | 25g | 56.00 | Add to Cart |
Methyl Violet
2B, Certified, C.N. #DcMv-5
(Basic Violet 1; Dahlia B; Gentian Violet;
Paris Violet; Pyoktanium Coeruleum)
C24H28N4Cl F.W. 393.96 CAS
#8004-87-3
Solubility: 9.0% Water; 8.25% Alcohol; 9.0% Cellosolve; 8.5% Glycol;0.01%
Xylene
Dye content: 75% min
Used with Methyl Green to stain Epoxy-embedded sections.
| RT | 18850 | 25g | 42.00 | Add to Cart |
Nigrosin
(Acid Black 2; Gray R, B, BB; Indulin Black; Nigrosin W, WL
; Silver Gray; Steel Gray)
CAS #8005-03-06
Solubility: 10.0% Water; 0.0% Alcohol; 1.0% Cellosolve; 11.0% Glycol; 0.0%
Xylene
For negative staining of bacteria. With Basic Fuchsin, staining bacteria
spores. Dorner, Le Lait, 6, 8 (1926). Biochem. Biophys. Acta 69,159 (1963).
| RT | 19010 | 25g | 34.00 | Add to Cart |
Nile Blue A
(Basic Blue 12, Nile Blue Sulfate)
(C20H20N3O)2SO4 F.W.
732.84 CAS #3625-57-8
Solubility: 6.0% Water; 5.0% Alcohol; 5.0% Cellosolve; 5.0%
Glycol; 0.25% Xylene.
Dye content: approx. 65%
Differentiates melamins and lipofuscins; Used in fat staining.
Lillie, Stain Tech., 31,151 (1956); J. Histochem., 4,377; Z. Anal. Chem.
260, 290 (1972).
For staining bone sections. Cytologia, 11, 261 (1940)
| RT | 19020 | 10g | 15.00 | Add to Cart |
Oil Red O, Certified, C.N. #DcRo-3
(Solvent Red 27, Sudan Red 5B)
C26H24N4O F.W. 408.49 CAS [1320-06-5]
Oil Red O is lysochrome (fat soluble dye) used demonstrate fat or lipids in fresh tissue sections. The polyazo group of dyes include the Oil Red, Sudan Red and the Sudan Blacks - all these dyes are interchangeable.
| RT | 19056 | 10g | 15.00 | Add to Cart |
Orange II
(Acid Orange 7; Acid Orange II, Y or A; Gold Orange; Mandarin
G; Orange A, P or R; Orange Extra; Tropaoelin OOO No.2)
C16H11N2O4SNa F.W.
350.33 CAS #633-96-5
Solubility: 3.0% Water; 0.15% Alcohol; 2.5% Cellosolve; 8.0% Glycol; 0.0%
Xylene
Dye Content: approx. 75%
A general tissue stain with Eosin and Azur-C. French, Stain Tech., 1, 79 (1926). Counterstain for Heidenhain's Hematoxylin in animal histology. A substitute for Orange G if a stronger yellow is desired for contrast. J. Lab. Med., 28, 995 (1943)
| RT | 19060 | 25g | 16.00 | Add to Cart |
Orange G, Certified, C.N. #DcO-13
(Acid Orange 10, Wood Orange 2G)
C16H10N2O7S2Na2 F.W..
452.38 CAS #1936-15-8
Solubility: 8.0% Water; 0.22% Alcohol; 1.7% Cellosolve; 3.0% Glycol; 0.0%
Xylene Dye Content: approx. 60%
Kornhauser's Quad stain for most elementary structures of tissues. Stain Tech., 18, 985 (1943) Mallory's tissue stain for connective tissue. Mallory, J. Exper. Med., 5,15 (1900). Used with safranin and tannic acid for showing shoot apex in plant tissue. Stain Tech., 18, 105 (1943).
| RT | 19070 | 25g | 18.00 | Add to Cart |
Phenol Red
(Phenolsulfonphthalein)
C19H14O5S F.W. 354.38 CAS
#143-74-8
A standard indicator for preparing buffers, pH range 6.4-8.2.
| RT | 19327 | 5 g | 18.00 | Add to Cart |
Phenol Red Sodium Salt
(Phenolsulfonphthalein Sodium Salt)
C19H13NaO5S F.W. 376.37 CAS
#34487-61-1
A standard indicator for preparing buffers, pH range 6.8-8.2.
| Assay | >99.0% | |
| Water | <0.1% |
| RT | 19328 | 5 g | 30.00 | Add to Cart |
Phloxine B, Certified, C.N.
#DcPh-6
(Acid Red 92; Cyanosine; Eosin 10B; Phloxine TA, N, BP
Super, RB, TB, or BB)
C20H2O5Cl4Br4Na2 F.W.
829.66 CAS #18472-87-2
Solubility: 10.5% Water; 5.0% Alcohol; 9.0% Cellosolve; 4.5% Glycol; 0.0% Xylene
| RT | 19350 | 25 g | 36.00 | Add to Cart |
Pyronin Y, Certified, C.N. #DcPy-2
(Pyronin G)
C17H19N2OCl F.W. 302.81 CAS #92-32-0
Solubility: 9.0% Water; 0.5% Alcohol; 1.85% Cellosolve; 4.35% Glycol; 0.0%
Xylene
| RT | 19560 | 5 g | 51.00 | Add to Cart |
Safranin
O, Certified, C.N. #DcS-25
(Safranin T, Y or A; Basis Red 2; Cotton Red; Gossypimine
C20H19N4Cl F.W. 350.85 CAS
#477-73-6
Solubility: 4.5% Water; 3.5% Alcohol; 5.0% Cellosolve; 3.5% Glycol; 0.0% Xylene
| RT | 20800 | 25 g | 30.00 | Add to Cart |
Sudan
Black B, Certified, C.N. #DcZb-2
(Solvent Black 3)
C29H24N6 M.W. 456.56 CAS
#4197-25-5
Solubility: Water-insoluble; Alcohol-soluble; 4.0% Cellosolve; 1.0% Glycol.
| RT | 21610 | 25 g | 27.00 | Add to Cart |
Toluidine Blue 0, Certified, C.N.
#DcU-10
(Basic blue; Methylene Blue T50 or T Extra)
C15H16N3SCl FW 305.83 CAS #92-31-9
Dye Content: approx. 85% min.
Solubility: 3.25% Water; 1.75% Alcohol; 3.5% Cellosolve; 5.5% Glycol; 0.0%
Xylene
*-A general stain for epoxy thick sections. Barajas et al.(1981) Identification
of renal neuroeffector junctions by electron microscopy of reembedded light
microscopic autoradiograms of semithin sections. J. Ultrastruct. Res. 77: 379.Campbell,
R.D., And Hermans, C.O. (1972). A rapid method for resectioning 0.5 4.0 micron
epoxy sections for electron microscopy. Stain Technol . 47:115.
| RT | 22050 | 50g | 36.00 | Add to Cart |
Wright Stain, Certified #DcWr-38
CAS #68988-92-1
Solubility: 0.091% Water; 0.23% Alcohol
| RT | 22720 | 25 g | 28.00 | Add to Cart |
