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Electron Microscopy Sciences

Histology and Light Microscopy

arrow14Certified Dyes/Stains

msdsarrowAcid Fuchsin, Certified, C.N. #DcR-8

(Rubin S, Fuchsin S, Fuchsin Acid, Acid Magenta, Acid Violet 19) C20H17N3Na2O9S3   F.W. 585.54   CAS #3244-88-0 Solubility: 0.053% Water; 1.21% Alcohol

RT 10035   25 g 30.00 Add to Cart

msdstechnical data sheetarrowAcridine Orange

(Basic Orange 14) C17H2ON3 Cl   F.W. 301.82   CAS #65-61-2

Ultrastructural probes on DNA templates within human bone marrow and lymph node cells. Frenstar, J., Adv. Cell Mol. Biol., 3, 1(1974). Used to detect changes in DNA template activity within cells. Frenster, J. Cell Nucl., 1, 565 (1974) Fluorescent dye for cytochemistry. J. Histochem. Cytochem., 31, 737 ((1983) J. Lab. Med., 15 (3),180, (1984)

RT 10050   25g 62.00 Add to Cart

msdsarrowAlcian Blue 8GX, C.N. #AnAn-6 (Ingrain Blue I)

C56H68N16S4Cu   F.W. 1298.88   CAS #12040-44-7 Dye Content: Approx. 50% Solubility: Water-soluble

Used as a fixative: 0.5-2% Alcian Blue in Glutaraldehyde fixative enhances the preservation and constrast of the cell coat and intercellular substances. Used as a stain: For staining cell surface materials and cartilage components. Schofield, et al., Histochem J. 7, 139,1975 Used as a wetting agent: Sommer, J. R (1977). To cationize glass. J.Cell Bio. 75, 245a. Alcian Blue and Ruthenium Red were used to study cartilage ultrastructure. Thyberg, J., Lohmander, S., and Friberg, U., J. Ultrastruct. Res., 45, 407 (1973).

RT 10350   25g 190.00 Add to Cart

Technical Tip

Purification of Alcian Blue

msdsarrowAlizarin Red S, Certified, C.N. #DcAr-5

(Alizarin Camine, Mordant Red 3) C14H7O7SNa.H2O   F.W. 360.28   CAS #130-22-3 Solubility: 7.69% Water; 0.1% Alcohol.

Certified for Staining Bone. Stain for nervous tissue in small invertebrates, and for sections of nervous tissue. Lee, "The Microtomist's Vade-Mecum", 10th Ed., The Blakiston Co., Phila., 1937; Dawson, Stain Tech., 1, 123 (1926); Lundvall, Anat. Anz., 27, 521 (1905); 137. Use to differentiate bone from cartilage in mammalian embryos; used with Toluidine Blue. William, Stain Tech., 16, 23 (1941)

RT 10360   25g 39.00 Add to Cart

msdsarrowAniline Blue Disodium Salt, Water Soluble, Certified, C.N. #DcK-11

(Acid Blue 22; China Blue; Cotton Blue; Marine Blue V; Soluble Blue 3M, 2R; Water Blue) C32H25N3O9S3Na2   F.W. 737.74   CAS #28631-66-5

Solubility: 50% Water; 0.0% Alcohol; 4.5% Cellosolve; 7.8% Glycol; 0.0% Xylene Used in connective tissue staining procedures to stain collagen. Used in differential staining. Snodgress el al., Lab Invest. 26:329, 1972.

RT 10840   25g 32.00 Add to Cart

msdsarrowAuramine O, Certified, C.N. # DcAu-4

(Pyoctanunum aureum; Basic Yellow 2; Pyoktanin Yellow; Canary Yellow) C17H21N3HCl·H2O   F.W. 321.86   CAS # 2465-27-2 Solubility: 1.0% Water; 4.0% Alcohol; 1.2% Cellosolve; 1.75% Glycol; 0.05% Xylene

RT 10850   25 g 78.00 Add to Cart

msdsarrowAzocarmine G

(Acid red 101; Basalan Carmine GE; Rosinduline) C28H17N3Na2O6S2   F.W. 601.57   CAS #25641-18-3 Solubility: 1.0% Water; 0.1% Alcohol; 1.75% Cellosolve; 4.5% Glycol; 0.01% Xylene

RT 11150   5 g 37.00 Add to Cart

msdsarrowAzur A (Methylene Azur A)

C14H14ClN3S   F.W. 291.80   CAS #531-53-3 Solubility: Water-soluble; Alcohol-soluble Used in nuclear staining; A polychromatic blood stain.

RT 11240   10g 80.00 Add to Cart

msdsarrowAzur B (Azur I; Methylene Azur B)

C15H16N3SBr   FW 350.29   CAS #531-55-5

Solubility: Water-soluble; Alcohol-slightly soluble. An important component of Giemsa's stain for blood protozoa. Used for staining semi-thin sections of plant tissue. Hoefert, L.L. (1968). Polychromatic stains for thin sections of Beta embedded in epoxy resin. Stain Tech. 43:145

RT 11200   10g 52.00 Add to Cart

msdsarrowAzur II

(Azur B/Methylene Blue 1:1)   CAS #37247-10-2

  11210   10g 38.00 Add to Cart

msdsarrowAzur II Eosin

(Azur B/Methylene Blue/Eosin 1:1:1)   CAS #53092-85-6

RT 11220   10g 56.00 Add to Cart

msds arrowBasic Fuchsin, Certified, C.N. #DcFb-26

(Rasaniline Chloride; Magenta; Basic Violet 14) C20H19N3.HCl   F.W. 337.86   CAS #632-99-5

Berkowitz et al., (1968). Selective staining of nervous tissue for light microscopy following preparation for electron microscopy. J. Histochem. Cytochem. 16:808. Sato, T., and Shamoto, M (1973). A simple rapid polychrome stain for epoxy-embedded tissue. Stain Technol. 48:223.

RT 11260   25g 80.00 Add to Cart

msdsarrowBasic Green 4, Certified, C.N. #DcMg-11

(Malachite Green Oxalate; Diamond Green B; BX or P Extra; Light Green N, Solid Green O; Victoria Green B or WB) 2(C23H25N2).2(C2H2O4).C2O4   F.W. 927.03   CAS #2437-29-8 Solubility: 10.0% Water; 8.5% Alcohol; 5.5% Cellosolve; 7.0% Glycol; 0.0% Xylene. Used as a bacteria stain and a counterstain for botanical material.

RT 18100   25g 42.00 Add to Cart

msds arrowBiebrich Scarlet, Water Soluble

(Bonceau BS; Acid Red 66; Croceine Scarlet; Double Scarlet BSF) C22H14N4Na2O7S2   F.W. 556.4   9 CAS #4196-99-0 Solubility: 5.0% Water; 0.25% Alcohol; 1.5% Cellosolve; 1.5% Glycol; 0.0% Xylene

RT 11265   25g 28.00 Add to Cart

msdsarrowBismarck Brown Y

(Basic Brown 1; Basic Brown G, GX, GXP; Vesuvine; Leather Brown; Manchester Brown; Phenylene Brown) C18H18N8·2HCl   F.W. 419.32   CAS #10114-58-6 Solubility: 1.5% Water; 3.0% Alcohol; 3.0% Cellosolve; 7.9% Glycol; 0.05% Xylene

RT 11310   25g 20.00 Add to Cart

msdsarrowBrilliant Cresyl Blue, Certified, C.N.#DcV-5

(Brilliant Blue C; Cresyl Blue 2RN) C17H21N4OCl   F.W. 332.84   CAS #10127-36-3 Solubility: 3.0% Water; 2.6% Alcohol; 2.25% Cellosolve; 8.0% Glycol; 9.5% Xylene

RT 11465   10g 52.00 Add to Cart

msdsarrowBromophenol Blue, Reagent, A.C.S.

(3',3",5 '5"-Tetrabromophenol-sulfonthalein) C19H10O5SBr4   F.W. 669.97   CAS #115-39-9

  11470   5g 25.00 Add to Cart
  11471   25g 88.00 Add to Cart

msdsarrow12Carmine, Certified, C.N.#DcCa-16

Alum Lake of Carminic acid
(Natural Red 4)
CAS # 1390-65-4
Solubility: 0.0% Water; 0.2% Alcohol; 0.5% Cellosolve; 4.0% Glycol; 0.0% Xylene.

RT 12410   10 g 29.00 Add to Cart

msdsarrow12Cresyl Fast Violet, Certified, C.N.# DcW-5

(Cresyl Violet Acetate)
C18H15N3O3   F.W. 312.34   CAS # 10510-54-0
Solubility: 0.13% water

RT 12780 Cresyl Fast Violet, Certified, 5 g 78.00 Add to Cart

msdsarrow12Crystal Violet, Certified, C.N. #DcC-38

(Basic Violet 3; Gentian Violet; Methyl Violet 10B; Hexamethyl Violet)
C25H30N3Cl   F.W. 407.99   CAS #548-62-9
Solubility: 9.0% Water; 8.75% Alcohol; 7.5% Cellosolve; 7.0% Glycol; 0.0% Xylene

RT 12785 Crystal Violet 25 g 22.00 Add to Cart

msdsarrow12Eosin Y, Certified, C.N. #DcE-40

(Acid Red 87, Eosin Yellowish)
C2cH6Br4Na2O5   F.W. 691.88   CAS #17372-87-1

Solubility: 44.0% Water; 2.0% Alcohol; 25.0% Cellosolve; 27.5% Glycol; 0.0% Xylene.
Used with Hematoxylin for showing cell nuclei in embryos and kidneys. Conn, "Biological Stain" 9th ed., The Williams and Wilkens Co., Baltimore 1977, pg. 7.

Component of Wright's stain for blood corpuscles. Wright, J. Med. Res., 7, 138 (1902)
For staining bone marrow to show cell morphology. Bech, "Lab. Manual of Hematological Tech.", W. B. Saunders Co., Phila., 1908, p. 233

RT 14850   10g 10.00 Add to Cart
RT 14851   25g 20.00 Add to Cart

msdsarrow12Erythrosin B, Certified, C.N. #DcEr-5

(Acid Red 51, Erythrosin extra Bluish; Eosin J; Iodeosin B; Pyrosin B)
C20H6O5I4Na2   F.W. 879.87   CAS #16423-68-0
Solubility: 10.0% Water; 5.0% Alcohol; 7.0% Cellosolve; 8.25% Glycol; 0.0% Xylene.

RT 15010   10 g 23.00 Add to Cart

msdsarrow12Fast Green FCF, Certified, C.N. #DcGf-12

(Food Green 3)
C37H34O10N2S3Na2   F.W.808.86   CAS #2353-45-9
Solubility:4.0% Water; 9.0% Alcohol; 8.0% Cellosolve; 6.25% Glycol; 0.0% Xylene

The main advantage of this dye is its resistance to fading. Mammalian tissue stain for visualizing collagen, muscle, cystoplasma, cells and cornified epithelium. Kornhauser, Stain Tech., 18,95 (1943); 20, 23 (1945); Lillie, Arch. Path., 29, 705 (1940). Modified Feulgen stain for visualizing chromosomes in root tips and other meristematic tissue. DeTomasi, Stain Tech., 11, 137 (1936). Used in plant histology. O. Conn, "Biological Stains" 9th ed., Williams & Wilkens Co., Baltimore, 1977, pp. 252-3; Haynes, Stain Tech., 3, 40 (1928).

RT 15500   5g 22.00 Add to Cart

msdsHistocryl Resin arrow12Giemsa Solution, C.N.#DcGe-19

Glycerin (CAS#: 56-81-5): 61%
Methyl Alcohol (CAS#: 67-56-1): 38.3%
Azur II (CAS#: 37247-10-2): 0.16%
Azur II Eosin (CAS#: 53092-85-6): 0.06%

Specific Gravity: 0.89
Differentiates types of leucocytes, bacteria and inclusion bodies.
Giemsa, Centralbl. F. Publ., Abt. 1,37, 308 (1904) Stain for thick blood films; shows malarial parasites. Barber and Komp, Publ. Health Rep., 44, 2330 (1929) Bone marrow and stain for showing cell morphology; together with May-Grunwald Solution for pap staining. Pappenheim, Folia Haemat., 13, 338 (1912)

RT 15940 Giemsa Stain Solution 100 ml 20.00 Add to Cart

msdsarrow12Giemsa Stain, C.N.#DcGe-19, Powder

CAS #51811-82-6
See the listing in Certified Dyes/Stains

RT 15942   10g 40.00 Add to Cart

msds arrow12Hematoxylin, Certified, C.N.#DcH-48

(Natural Black I, Hydroxybrasilin)
C16H14O6   F.W. 302.29   CAS #517-28-2
Solubility: 10.0% Water; 10.0% Alcohol; 9.5% Cellosolve; 10% Glycol; 0.0% Xylene. A certified biological stain used in histology and cytology and plastic embedded tissues.
Chen, Chang, S., J Arch. of Path., 93,334 (1972).

RT 16620   10g 28.00 Add to Cart

Technical Tip

Hematoxylin Staining Tips

msdsarrow12Indigo Carmine, Certified, C.N. #DcI-7

(Acid Blue 74; Carmine Blue; Indigotine 1, 1A)
C16H8N2O8S2Na2   F.W.466.36   CAS #860-22-0
Dye content: approx. 85%
Solubility: 1.3% Water; 0.0% Alcohol; 4.0% Cellosolve; 2.85% Glycol; 0.0% Xylene
Shunway's stain for animal embryos. Used with Acid Fuchsin for staining Negri bodies. Stain Tech., 5, 34 (1928)
Counterstain to Hematoxylin for staining vaginal smears. Am. J. Anat., 61, 505 (1937).

RT 16910   25g 17.00 Add to Cart

Technical Tip

When a stain is applied both en-bloc and as a post-stain for thin sections, maximum contrast will be achieved.

msdsarrow12Indium Trichloride, Anhydrous

InCl3   F.W. 221.18   CAS #10025-82-8
Chloride Content: 98%
A metal stain for nucleic acid
J. Biophys Biochem Cytol, 11,257 (1961)

RT 16920   5g 60.00 Add to Cart

msdsarrow12Light Green SF Yellowish, Certified, C.N. #DcL-30

 

(Acid Green 5; Light Green 2G, S or 2GN; Acid Green F; Fast Acid Green N)
C37H34N2Na2O9S3   F.W. 792.86   CAS #5141-20-8
Solubility: 20.35% Water; 4.0% Alcohol; 5.25% Cellosolve; 12.0% Glycol; 0.0% Xylene

RT 17920 10 g 15.00 Add to Cart

msdsarrowMalachite Green 4, Certified, C.N. #DcMg-11

(Malachite Green Oxalate; Diamond Green B; BX or P Extra; Light Green N, Solid Green O; Victoria Green B or WB) 2(C23H25N2).2(C2H2O4).C2O4   F.W. 927.03   CAS #2437-29-8 Solubility: 10.0% Water; 8.5% Alcohol; 5.5% Cellosolve; 7.0% Glycol; 0.0% Xylene. Used as a bacteria stain and a counterstain for botanical material.

RT 18100   25g 42.00 Add to Cart

msdsarrow12Methylene Blue Chloride, Certified, C.N. #DcA-24

(Basic Blue 9; Swiss Blue)
C16H18N3SCl •3H2O  F.W. 319.86+aq   CAS #7220-79-3
Solubility: 9.5% Water; 6.0% Alcohol; 6.5% Cellosolve; 10.0% Glycol; 0.0% Xylene.

  • Used to distinguish autonomic nerves. Richardson, K.C., (1969) Anat. Rec., 164, 231. General stain for animals, histology.
  • A simple rapid staining procedure for methacrylate embedded tissue sections using chromotrope 2R and Methylene Blue. Dougherty, M.M., and King, J.S., (1984) Stain Tech., 59, 149
  • An established routine method for differential staining of epoxy-embedded tissue sections. Schroeder, H.E., Rossinsky, K., and Muller, W., (1980) Microsc. Acta 83, 111
  • Used with Basic Fuchsin for stain epoxy-embedded tissue. Sato, T. and Shamoto, M. (1973). A simple rapid polychrome stain for epoxy-embedded tissue. Stain Technol., 48:223
RT 18600 25g 38.00 Add to Cart

msdsarrow12Methyl Green, Certified, C.N. #DcG-10

(Zinc Chloride Salt; Ethyl Green)
C27H35N3C12·ZnC12   F.W. 608.79   CAS #7114-03-6
Solubility: 8.0% water; 3.0% Alcohol; 1.8% Cellosolve; 3.5% Glycol; 0.0% Xylene.

  • Used as a counterstain in Almann's aniline acid fuchsin technique for mitochrondria; used in Kurnick's Methyl Green-Pyonin stain for nuclei and cystoplasmic granules of liver cells. Marker for DNA at acid and neutral conditions. J.B. Boyd, H.R. Mitchell, (1965) Anal. Biochem., 13, 28.
  • Used for epoxy-embedded thick sections. Sievers, J. (1971).
  • Basic two-dye stains for epoxy-embedded 0.3-1 micron sections. Stain Technol., 46:195
RT 18700 25g 56.00 Add to Cart

msdsarrow12Methyl Violet 2B, Certified, C.N. #DcMv-5

(Basic Violet 1; Dahlia B; Gentian Violet; Paris Violet; Pyoktanium Coeruleum)
C24H28N4Cl   F.W. 393.96   CAS #8004-87-3
Solubility: 9.0% Water; 8.25% Alcohol; 9.0% Cellosolve; 8.5% Glycol;0.01% Xylene
Dye content: 75% min
Used with Methyl Green to stain Epoxy-embedded sections.

RT 18850 25g 42.00 Add to Cart

msdsarrow12Nigrosin

(Acid Black 2; Gray R, B, BB; Indulin Black; Nigrosin W, WL ; Silver Gray; Steel Gray)
CAS #8005-03-06
Solubility: 10.0% Water; 0.0% Alcohol; 1.0% Cellosolve; 11.0% Glycol; 0.0% Xylene
For negative staining of bacteria. With Basic Fuchsin, staining bacteria spores. Dorner, Le Lait, 6, 8 (1926). Biochem. Biophys. Acta 69,159 (1963).

RT 19010   25g 34.00 Add to Cart

msdsarrow12Nile Blue A

(Basic Blue 12, Nile Blue Sulfate)
(C20H20N3O)2SO4   F.W. 732.84   CAS #3625-57-8

Solubility: 6.0% Water; 5.0% Alcohol; 5.0% Cellosolve; 5.0% Glycol; 0.25% Xylene.
Dye content: approx. 65%
Differentiates melamins and lipofuscins; Used in fat staining.
Lillie, Stain Tech., 31,151 (1956); J. Histochem., 4,377; Z. Anal. Chem. 260, 290 (1972).
For staining bone sections. Cytologia, 11, 261 (1940)

RT 19020   10g 15.00 Add to Cart

msdsarrow12Oil Red O, Certified, C.N. #DcRo-3

(Solvent Red 27, Sudan Red 5B)
C26H24N4O   F.W. 408.49   CAS [1320-06-5]

Oil Red O is lysochrome (fat soluble dye) used demonstrate fat or lipids in fresh tissue sections. The polyazo group of dyes include the Oil Red, Sudan Red and the Sudan Blacks - all these dyes are interchangeable.

RT 19056   10g 15.00 Add to Cart

msdsarrow12Orange II

(Acid Orange 7; Acid Orange II, Y or A; Gold Orange; Mandarin G; Orange A, P or R; Orange Extra; Tropaoelin OOO No.2)
C16H11N2O4SNa   F.W. 350.33   CAS #633-96-5
Solubility: 3.0% Water; 0.15% Alcohol; 2.5% Cellosolve; 8.0% Glycol; 0.0% Xylene
Dye Content: approx. 75%

A general tissue stain with Eosin and Azur-C. French, Stain Tech., 1, 79 (1926). Counterstain for Heidenhain's Hematoxylin in animal histology. A substitute for Orange G if a stronger yellow is desired for contrast. J. Lab. Med., 28, 995 (1943)

RT 19060   25g 16.00 Add to Cart

msdsarrow12Orange G, Certified, C.N. #DcO-13

(Acid Orange 10, Wood Orange 2G)
C16H10N2O7S2Na2   F.W.. 452.38   CAS #1936-15-8
Solubility: 8.0% Water; 0.22% Alcohol; 1.7% Cellosolve; 3.0% Glycol; 0.0% Xylene Dye Content: approx. 60%

Kornhauser's Quad stain for most elementary structures of tissues. Stain Tech., 18, 985 (1943) Mallory's tissue stain for connective tissue. Mallory, J. Exper. Med., 5,15 (1900). Used with safranin and tannic acid for showing shoot apex in plant tissue. Stain Tech., 18, 105 (1943).

RT 19070   25g 18.00 Add to Cart

msdsarrow12Phenol Red

(Phenolsulfonphthalein)
C19H14O5S   F.W. 354.38   CAS #143-74-8
A standard indicator for preparing buffers, pH range 6.4-8.2.

RT 19327   5 g 18.00 Add to Cart

msdsarrow12Phenol Red Sodium Salt

(Phenolsulfonphthalein Sodium Salt)
C19H13NaO5S   F.W. 376.37    CAS #34487-61-1
A standard indicator for preparing buffers, pH range 6.8-8.2.

Assay   >99.0%
Water   <0.1%
RT 19328   5 g 30.00 Add to Cart

msdsarrow12Phloxine B, Certified, C.N. #DcPh-6

(Acid Red 92; Cyanosine; Eosin 10B; Phloxine TA, N, BP Super, RB, TB, or BB)
C20H2O5Cl4Br4Na2   F.W. 829.66   CAS #18472-87-2
Solubility: 10.5% Water; 5.0% Alcohol; 9.0% Cellosolve; 4.5% Glycol; 0.0% Xylene

RT 19350   25 g 36.00 Add to Cart

msdsarrow12Pyronin Y, Certified, C.N. #DcPy-2

(Pyronin G)
C17H19N2OCl  F.W. 302.81  CAS #92-32-0
Solubility: 9.0% Water; 0.5% Alcohol; 1.85% Cellosolve; 4.35% Glycol; 0.0% Xylene

RT 19560   5 g 51.00 Add to Cart

msdsSafranin O, Certified, C.N. #DcS-25

(Safranin T, Y or A; Basis Red 2; Cotton Red; Gossypimine
C20H19N4Cl   F.W. 350.85   CAS #477-73-6
Solubility: 4.5% Water; 3.5% Alcohol; 5.0% Cellosolve; 3.5% Glycol; 0.0% Xylene

RT 20800   25 g 30.00 Add to Cart

msdsSudan Black B, Certified, C.N. #DcZb-2

(Solvent Black 3)
C29H24N6   M.W. 456.56   CAS #4197-25-5
Solubility: Water-insoluble; Alcohol-soluble; 4.0% Cellosolve; 1.0% Glycol.

RT 21610   25 g 27.00 Add to Cart

msdsarrowToluidine Blue 0, Certified, C.N. #DcU-10

(Basic blue; Methylene Blue T50 or T Extra)
C15H16N3SCl   FW 305.83   CAS #92-31-9
Dye Content: approx. 85% min.
Solubility: 3.25% Water; 1.75% Alcohol; 3.5% Cellosolve; 5.5% Glycol; 0.0% Xylene
*-A general stain for epoxy thick sections. Barajas et al.(1981) Identification of renal neuroeffector junctions by electron microscopy of reembedded light microscopic autoradiograms of semithin sections. J. Ultrastruct. Res. 77: 379.Campbell, R.D., And Hermans, C.O. (1972). A rapid method for resectioning 0.5 4.0 micron epoxy sections for electron microscopy. Stain Technol . 47:115.

RT 22050   50g 36.00 Add to Cart

msdsarrowWright Stain, Certified #DcWr-38

CAS #68988-92-1
Solubility: 0.091% Water; 0.23% Alcohol

RT 22720   25 g 28.00 Add to Cart

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