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Electron Microscopy Sciences

Histology and Light Microscopy

arrow13Prepared Staining Kits for Histology and Pathology

F. CONNECTIVE TISSUE STAINS

F.1 Van Gieson Stains and related

Alcoholic Stain SolutionsarrowVan Gieson's Method for Collagen Fibers

Mallory (1938); Van Gieson (1889)

For collagen, muscle and cornified epithelium.

Stain results:

Collagen:   Red
Smooth and striated muscle:   Yellowish to Brownish
Cornified epithelium:   Yellow
Hyalin:   Yellow
Nuclei:   Blue to Black

References:

  • Clark, G., (ed) Staining Procedures, 3rd Ed., Williams & Wilkins Co., Baltimore, c. 1973, p. 50
  • Luna, L. (ed) AFIP Manual o f Histological Staining Methods, 3rd ED., New York: McGraw Hill Publications, c 1968, p. 76
  • Van Gieson, I., New York Med . J., 50:57-60, 1889.
  • Mallory, F.B., Pathological Technique, Hafner Publishing., New York, c. 1961, p. 152
RT 26350-01 Van Gieson's Solution msds 500 ml 32.00 Add to Cart
RT 26350-02 Weigert's Iron Hematoxylin A msds 500 ml 40.00 Add to Cart
RT 26350-03 Weigert's Iron Hematoxylin B msds 500 ml 25.00 Add to Cart

Alcoholic Stain SolutionsarrowPuchtler-Sweat Method for Basement Membranes

Puchtler and Sweat (1964)

Stain results:

Basement Membranes:   Black in cross section/
Gray in tangential sections
Nuclei:   Pink to Red

References:

  • Puchtler, H. and Sweat, F.: Stain Technology, 39:163, 1964
  • AFIP Manual of Histological Staining Mehtods, 3rd ed., Ed. L. Luna: NewYork, McGraw Hill Pub., c. 1968, p. 73
RT 26356-01 Resorcin-Fuchsin Solution msds 500 ml 45.00 Add to Cart
RT 26356-02 Nuclear Fast Red (Kernechtrot) Solution msds 500 ml 44.00 Add to Cart
RT 26356-03 Periodic Acid, 0.5% Aqueous   500 ml 23.00 Add to Cart
RT 26356-04 Sodium Bisulfite Solution   500 ml 30.00 Add to Cart

Alcoholic Stain SolutionsarrowPicrosirius Red Staining of Cardiac

Muscle Following Phosphomolybdic Acid Treatment

For connective tissue stains – Van Gieson stains and variants.

Stain results:

Septa:   Red

References:

  • Dolber, Paul C. and Madison S. Spach: Picrosirus Red Staining of Cardiac Muscle Following Phosphomolybdic Acid Treatment, Stain Technology, Vol. 62 No. 1 (1987) p. 23-26
RT 26357-01 Phosphomolybdic Acid, 0.2% Aqueous msds 500 ml 16.00 Add to Cart
RT 26357-02 Sirius Red, 0.1% in Saturated Picric Acid msds 100 ml 8.00 Add to Cart
RT 26357-03 Hydrochloric Acid, 0.01N msds 500 ml 16.00 Add to Cart

F.2 Stains with Poly-Acid Step

Alcoholic Stain SolutionsarrowLillie Modification of Masson's Trichrome

Lillie (1940)

Stains cells, cytoplasm muscle and collagen of mammalian tissue

Stain results:

Nuclei:   Black
Cystoplasm:   Brown to Pink
Muscle:   Red
Erythrocytes:   Brilliant Scarlet
Collagen:   Blue to Blue Green
Myelinated fibers:   Red
Fibrin:   Red to Pink

References:

  • Clark, G.: Staining Procedures. Williams & Wilkins Co., Baltimore, 3rd ed., c. 1973, p. 59
  • Lillie, R.D., Arch. Path., 29:705, 1940
RT 26364-01 Phosphomolybdic/ Phosphotungstic Acid Solution msds 500 ml 65.00 Add to Cart
RT 26364-02 Weigert's Iron Hematoxylin A msds 500 ml 45.00 Add to Cart
RT 26364-03 Weigert's Iron Hematoxylin B msds 500 ml 27.00 Add to Cart
RT 26364-04 Biebrich Scarlet Solution, 1% msds 500 ml 23.00 Add to Cart
RT 26364-05 Fast Green FCF Solution, 2.5% msds 500 ml 45.00 Add to Cart
RT 26364-06 Acetic Acid, 1% Aqueous msds 500 ml 17.00 Add to Cart
RT 26364-07 Lugol's Iodine msds 500 ml 20.00 Add to Cart
RT 26364-7A Gram's Iodine Solution msds 500 ml 19.00 Add to Cart
RT 26364-08 Sodium Thiosulfate, 5% Aqueous msds 500 ml 19.00 Add to Cart

Alcoholic Stain SolutionsarrowMasson's Trichrome for Connective Tissue

Sheehan and Hrapchak (1980)

For stain nuclei, agentaffen granules, collagen, cytoplasm, keratin, etc.

Stain results:

Nuclei:   Black
Cytoplasm, Keratin, Muscle Fibers:   Red
Collagen, Mucin:   Blue

References:

  • Sheehan and Hrapchak: Theory and Practice of Histotechnology, St. Louis. The Moxby Company 1980, p. 190
RT 26367-01 Bouin's Fixative msds 500 ml 24.00 Add to Cart
RT 26367-02 Weigert's Iron Hematoxylin A msds 500 ml 41.00 Add to Cart
RT 26367-03 Weigert's Iron Hematoxylin B msds 500 ml 30.00 Add to Cart
RT 26367-04 Biebrich Scarlet Solution, 1% msds 500 ml 39.00 Add to Cart
RT 26367-05 Phosphomolybdic/ Phosphotungstic Acid Solution msds 500 ml 64.00 Add to Cart
RT 26367-06 Aniline Blue Solution msds 500 ml 60.00 Add to Cart
RT 26367-07 Acetic Acid, 1% Aqueous msds 500 ml 16.00 Add to Cart

F.3 Elastic Tissue Stains

Alcoholic Stain SolutionsarrowModified Verhoff Elastic Van Gieson Stain

For connective tissue stains – Stain with a poly-acid step.

Stain results:

Nuclei:   Grayish-Black
Collagen Fibers:   Red
Elastic Fibers:   Bluish-Black
Muscle:   Greenish-Yellow

References:

  • Gravey, Winsome et al "A modified Verhoff Elastic Van Gieson Stain" The Journal of Histotechnology, Vol. 14, No. 2, pp 113-114. June 1991
RT 26369-01 Alcoholic Hematoxylin, 3% msds 500 ml 61.00 Add to Cart
RT 26369-02 Ferric Chloride, 2% aqueous msds 500 ml 18.00 Add to Cart
RT 26369-03 Lugol's Iodine msds 500 ml 22.00 Add to Cart
RT 26369-04 Ferric Chloride, 0.4% aqueous msds 500 ml 15.00 Add to Cart
RT 26369-05 Van Geison's Solution msds 250 ml 21.00 Add to Cart

Alcoholic Stain SolutionsarrowWeigert's Resorcin Fuchsin

Lillie (1965); Mallory (1938); Weigert (1898)

For stain elastic fibers in blood vessel walls, nuclei and collagen.

Stain results:

Elastic Tissues:   Dark Blue
Nuclei (red if Orth's Carmine used):   Dark Blue
Collagen:   Red-Pink
Miscellaneous Tissue Elements:   Yellow (with Van Gieson's stain)

References:

  • Mallory, F.B.: Pathological Technique; New York: Hafner Publishing Co., c. 1961, p. 168
  • Clark G.: Staining Procedures, Baltimore: Williams & Wilkins Co., 3rd ed. c. 1973, p. 60
  • Luna, I.G.: AFIP Histologic Staining Methods: New York: McGraw Hill Book Co., 3rd ed., c. 1968, p. 80
RT 26370-01 Resorcin-Fuchsin Solution msds 500 ml 43.00 Add to Cart
RT 26370-02 Van Gieson's Solution msds 500 ml 31.00 Add to Cart
RT 26370-03 Weigert's Iron Hematoxylin A msds 500 ml 40.00 Add to Cart
RT 26370-04 Weigert's Iron Hematoxylin B msds 500 ml 26.00 Add to Cart
RT 26370-05 Acid Alcohol, 1% msds 500 ml 20.00 Add to Cart

Alcoholic Stain SolutionsarrowVerhoeff's Van-Gieson's (VVG Method)

Sheehan and Hrapchak (1980)

For stain elastic fibers, nuclei, collagen and other tissue elements.

Stain results:

Elastic Fibers:   Blue-black to Black – Fine elastic fibrils
may not be stained with this method
Nuclei:   Blue to Black
Collagen:   Red
Other Tissue Elements:   Yellow

References:

  • Sheehan, Dezna C., Hrapchack, Barbara B.: Theory and Practice of Histotechnology, 2nd ed. The C.V. Mosby C., St Louis, p. 196-197, c. 1980
RT 26374-01 Alcoholic Hematoxylin, 5% msds 500 ml 57.00 Add to Cart
RT 26374-02 Ferric Chloride, 10% msds 500 ml 25.00 Add to Cart
RT 26374-03 Weigert's Iodine msds 500 ml 24.00 Add to Cart
RT 26374-04 Ferric Chloride, 2% msds 500 ml 21.00 Add to Cart
RT 26374-05 Sodium Thiosulfate, 5% Aqueous msds 500 ml 20.00 Add to Cart
RT 26374-06 Van-Gieson's Solution msds 500 ml 31.00 Add to Cart

Alcoholic Stain SolutionsarrowCarstairs Method for Fibrin and Platelets

Carstairs (1965)

For the staining of fibrin and platelets.

Stain results:

  48 hours or more Less than 48 hours
Fibrin: Bright Red Orange to Orange-Red
Platelets Gray blue to navy Light Gray
Collagen: Bright Blue Bright Blue
Muscle: Red Red
Red Blood Cells: Clear Yellow Red, Green or Yellow
RT 26381-01 Ferric Ammonium Sulfate, 5% Aqueous msds 500 ml 19.00 Add to Cart
RT 26381-02 Mayer's Hematoxylin msds 500 ml 40.00 Add to Cart
RT 26381-03 Picric Acid-Orange G Solution msds 500 ml 40.00 Add to Cart
RT 26381-04 Ponceau Acid Fuchsin Solution msds 500 ml 24.00 Add to Cart
RT 26381-05 Phosphotungstic Acid, 1% msds 500 ml 42.00 Add to Cart
RT 26381-06 Aniline Blue Solution msds 500 ml 47.00 Add to Cart

Alcoholic Stain SolutionsarrowModified Movat's Stain

For connective tissue stains – Elastic tissue stains.

Stain results:

Nuclei:   Black
Cytoplasm:   Red
Elastic Fibers:   Purple to Black
Collagen and Bone:   Yellow
Mucopolysaccharides:   Blue-Green
Muscle:   Red

References:

  • Movat, H.Z.: Demonstration of all connective tissue elements in a single section. Arch Path., 60:289-295, 1955
  • Roman N., et al: Orcein-hematoxylin in iodized ferric chloride as a stain for elastic tissue. Stain Technology, 42:199-202, 1967
RT 26385-01 Alcian Blue, 1% msds 100 ml 24.00 Add to Cart
RT 26385-02 Alkaline Alcohol msds 500 ml 23.00 Add to Cart
RT 26385-03 Orcein, 0.2% msds 250 ml 24.00 Add to Cart
RT 26385-04 Hematoxylin Alcoholic, 5% msds 250 ml 35.00 Add to Cart
RT 26385-05 Ferric Chloride, 10% msds 500 ml 23.00 Add to Cart
RT 26385-06 Lugol's Iodine msds 500 ml 22.00 Add to Cart
RT 26385-07 Woodstain Scarlet-Acid Fuchsin Working Solution msds 250 ml 15.00 Add to Cart
RT 26385-08 Acetic Acid, 0.5% msds 500 ml 16.00 Add to Cart
RT 26385-09 Phosphotungstic Acid, 5% msds 500 ml 39.00 Add to Cart
RT 26385-10 Alcoholic Saffron, 6% msds 100 ml 39.00 Add to Cart

Alcoholic Stain SolutionsarrowGoldner's Trichrome Method

For connective tissue stains – Elastic tissue stain. Stain with a poly-acid step.

Stain results:

Nuclear chromatin:   Brownish-Black
Cytoplasm:   Bright Red
Erythrocytes:   Orange
Muscle:   Red
Collagen:   Green

References:

  • Luna, Lee G. Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts. Johnson Printers, Downerts Grove, IL pp 151-152, c 1992
RT 26386-01 Bouin's Fluid msds 500 ml 26.00 Add to Cart
RT 26386-02 Weigert's Iron Hematoxylin A msds 500 ml 35.00 Add to Cart
RT 26386-03 Weigert's Iron Hematoxylin B msds 500 ml 28.00 Add to Cart
RT 26386-04 Ponceau Acid Fuchsin msds 100 ml 11.00 Add to Cart
RT 26386-05 Acetic Acid, 1%   500 ml 16.00 Add to Cart
RT 26386-06 Phosphomolybdic Acid Orange G Solution msds 250 ml 24.00 Add to Cart
RT 26386-07 Light Green Stock, 0.2% msds 500 ml 31.00 Add to Cart

F.4 Silver Methods

Alcoholic Stain SolutionsarrowWilder Modification of Bielschowsky's Method

Wilder (1935)

For the staining of Collagen and reticulum.

Stain results:

Reticulum Fibers (If beading is observed and/or a dark
background, replace the ammonium hydroxide silver

Nitrate solution and the reducing solution):   Black
Collagen:   Rose
Other Tissue Elements:   Red

References:

  • Wilder, H.C., Amer. J. Clin. Path.: 11:817, 1935
  • Mallory, F.B.: Pathological Techniques, Phil., W.B. Saunders & Co. c. 1938
  • AFIP Manual of Hitological Staining Techniques, NY, McGraw-Hill Publ., c. 1968/3rd ed, p. 92
RT 26392-01 Silver Nitrate, 10.2% Aqueous   250 ml 101.00 Add to Cart
RT 26392-02 Ammonium Hydroxide, Concentrate msds 500 ml 18.00 Add to Cart
RT 26392-03 Sodium Hydroxide, 3.1% Aqueous msds 500 ml 23.00 Add to Cart
RT 26392-04 Formalin, 40%, Neutralized msds 500 ml 27.00 Add to Cart
RT 26392-05 Harris Hematoxylin
OR
  500 ml 32.00 Add to Cart
RT 26392-5A Van-Gieson Stain   500 ml 31.00 Add to Cart
RT 26392-06 Nuclear Fast Red (Kernechtrot) Solution msds 500 ml 39.00 Add to Cart
RT 26392-07 Phosphomolybdic Acid, 10% Aqueous msds 500 ml 81.00 Add to Cart
RT 26392-08 Uranium Nitrate, 1% Aqueous msds 500 ml 137.00 Add to Cart
RT 26392-09 Gold Chloride, 0.2%, Aqueous   250 ml 52.00 Add to Cart
RT 26392-10 Sodium Thiosulfate, 5% Aqueous   500 ml 20.00 Add to Cart

Alcoholic Stain SolutionsarrowJones Method for Kidney

- Silver Method

For the staining of basement membranes, reticulum fibers, collagen and nuclei.

Stain results:

Basement membranes, reticulum fibers:   Black
Nuclei:   Blue
Cytoplasm, collagen, connective tissue:   Pink-Orange

References:

  • Jones, D.B., Amer. J. Path. 27:99 (1951)
  • AFIP Manual of Histological Staining Methods, 3rd Ed., Ed. L. Luna: NY: McGraw-Hill Publications, c. 1968, p. 97
RT 26396-01 Periodic Acid, 0.5%, Aqueous msds 500 ml 24.00 Add to Cart
RT 26396-02 Methenamine Solution, 3%, Aqueous msds 500 ml 26.00 Add to Cart
RT 26396-03 Silver Nitrate, 5% Aqueous Solution msds 250 ml 83.00 Add to Cart
RT 26396-04 Borate Buffer Working Solution msds 500 ml 25.00 Add to Cart
RT 26396-05 Gold Chloride, 0.2%, Aqueous msds 250 ml 53.00 Add to Cart
RT 26396-06 Harris's Hematoxylin msds 500 ml 33.00 Add to Cart
RT 26396-07 Alcoholic Eosin Y Staining Solution msds 500 ml 20.00 Add to Cart
RT 26396-09 Sodium Thiosulfate, 3%, Aqueous msds 500 ml 18.00 Add to Cart
RT 26396-11 Potassium Ferricyanide, 0.5%, Aqueous msds 500 ml 18.00 Add to Cart
RT 26396-12 Sodium Metabisulfite, 3%, Aqueous msds 500 ml 27.00 Add to Cart
RT 26396-13 Acid Alcohol, 1% msds 500 ml 20.00 Add to Cart
RT 26396-14 Ammonia Water, 0.3% msds 500 ml 13.00 Add to Cart

F.5 Keratin Stains

Alcoholic Stain SolutionsarrowDane's Method for Prekeratin, Keratin, and Mucin

Keratin stain method for the staining of keratin, prekeratin, acid mucopolysaccharides and nuclei.

Stain results:

Acid Mucopolysaccharides:   Blue
Prekeratin and Keranin:   Orange to Red
Nuclei:   Brown

References:

  • Dane, E.T., and Herman, D.L.: Stain Technology, 38:97, 1963
  • AFIP Manual of Histological Staining Methods, 3rd Ed., Ed L. Luna: New York: McGraw Hill Publications, c 1968, p. 83
RT 26401-01 Phloxine Solution, 1%, Aqueous msds 500 ml 25.00 Add to Cart
RT 26401-02 Alcian Blue Solution, 0.5% msds 250 ml 52.00 Add to Cart
RT 26401-03 Orange G Solution msds 500 ml 40.00 Add to Cart
RT 26401-04 Mayer's Hematoxylin msds 500 ml 50.00 Add to Cart

Cytoplasmic Granule Stains arrow13arrow13