Maldonado's Method For Pancreatic Islet Cells

For the staining of alpha, beta, D- and Exocrine cells of the islets of langerhans.

Stain results:

(Bouin's Fixation without Acetic Acid)

  Human Tissue
Alpha granules: Purple
Beta granules: Violet Blue
D-cell granules: Light blue with evident granules
Exocrine cells: Grayish blue with red granules

References:

  • Maldonado, R. and San Jose, H. Stain Techn., 42:11-13, 1967
  • Luna, L.G., (ed.), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw, N.Y. p. 105, c. 1968
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