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Electron Microscopy Sciences

Histology and Light Microscopy

arrow13Reagents for Histology: Fixing, Dehydrating, Clearing and Staining

msdsarrow11HISTOCHOICE® Clearing Agent

Histology Grade – Non flammable, replacement for Xylene for all tissue clearing needs.

RT 64114-01 Histochoice Clearing Agent 4 L 62.00 Add to Cart
RT 64114-04 Histochoice Clearing Agent 4x4 L 240.00 Add to Cart

msdsarrow11HISTOCHOICE™MB®* – The Molecular Biology Fixative

  • SAFE – Contains no Formaldehyde, Glutaraldehyde or Mercury.
  • NON-TOXIC – Is completely odorless and can be safely disposed of down the drain.

HISTOCHOICE™ MB® Tissue Fixative is the first fixative designed for the molecular biologist. It is specialty formulated to preserve antigenic sites for antibody probes and nucleic acid sites for immunohistochemistry. HISTOCHOICE™MB® replaces formaldehyde based, alcohol based, Zenkers, B-5, B-3, Bouin's and other fixatives with superior results. Tissue fixed in HISTOCHOICE™MB® exhibit vibrant staining, better nuclear and cytoplasmic detail, and will retain a crisp appearance even after long-term fixation.

Because this formula is designed specially for molecular biology applications, HISTOCHOICE™MB® fixed sections do not require pre-digestion or other recovery procedures to make important sites available.This means you spend less time preparing slides and more time doing research.

More than a fixative, HISTOCHOICE™MB® is a preservative that leaves antigens and nucleic acids in their native state, allowing binding for specific probes. Primary antibodies can often be diluted several-fold due to the increased number of preserved antigenic sites, saving cost on every slide you process.

HISTOCHOICE™MB® fixed tissue retains a much more natural look and feel than formalin fixed tissue. It is important to remember that these tissues are not fixed by formalin, but rather preserved by HISTOCHOICE™MB®.

RT 64115-01 HISTOCHOICE™MB® Fixative 1 L 60.00 Add to Cart
RT 64115-04 HISTOCHOICE™MB® Fixative 4 L 190.00 Add to Cart
RT 64115-44 HISTOCHOICE™MB® Fixative 4x4 L 695.00 Add to Cart

*HISTOCHOICE is registered name of Amresco

msdsmsdsarrow11Histo-Clear®/Histo-Clear II®

Histo-Clear® (Distilled essential oils – food grade) and Histo-Clear II® (A mixtureof Aliphatic Hydrocarbon and Distilled essential oils – food grade, reduced citrus odor) are excellent non-toxic clearing agents replacing Xylene.

RT 64110-01 Histo-Clear® 1 L 32.00 Add to Cart
RT 64110-04 Histo-Clear® 1 gal 86.00 Add to Cart
RT 64110-10 Histo-Clear® 5 gal 425.00 Add to Cart
RT 64111-01 Histo-Clear II® 1 L 20.00 Add to Cart
RT 64111-04 Histo-Clear II® 1 gal 70.00 Add to Cart
RT 64111-10 Histo-Clear II® 5 gal 316.00 Add to Cart

® is register name of National Diagnostics

msdsarrow11Hollande's Fixative

A modification of Bouin's solution. It is stable and will decalcify small bone specimens. Tissue that is fixed with Hollande's can be stained successfully with most stains, and the cupric acetate in the solution stabilizes red blood cell membranes and cosinophil and endocrine cell granules so that less lysis occurs than with Bouin's solution. Hollande's is widely used as a fixative for biopsy specimens of the gastrointestinal tract.

Thorough washing of the fixative prior to placing the specimen in a phosphate buffered formalin solution is necessary because the salts present in the solution will form an insoluble phosphate precipitate. Packaged in leak-proof histo-containers; prefilled ready-to-use.

  Catalog # Cup Size Filled Pack/cs Price
RT 64120-10 15 ml 10 ml 50 78.00 Add to Cart
RT 64120-20 30 ml 20 ml 50 98.00 Add to Cart
RT 64120-30 60 ml 30 ml 20 46.00 Add to Cart

Bulk packaging is available:

RT 64120-01 Hollande's Fixative 1 L 42.00 Add to Cart
RT 64120-04 Hollande's Fixative 4 L 160.00 Add to Cart

arrow11Hydrochloric Acid, Diluted, (w/v)

HCl F.W. 36.46 CAS 7647-01-0

RT 16762 Hydrochloric Acid, 1% Aqueous msds 500 ml 24.00 Add to Cart
RT 16763 Hydrochloric Acid, 2% Aqueous   500 ml 24.00 Add to Cart
RT 16765 Hydrochloric Acid, 10% Aqueous msds 500 ml 27.00 Add to Cart
RT 16766 Hydrochloric Acid, 20% Aqueous msds 500 ml 29.00 Add to Cart

arrow11Hydrogen Peroxide 30% Solution – Stabilized, Reagent, A.C.S.

H2O2   F.W. 34.01   CAS #7722-84-1

Assay   29-32%
Maximum of Impurities:    
Chloride(Cl)   0.0003%
Nitrate   0.0002%
Phosphate   0.0002%
Sulfate   0.0005%
Heavy Metal   0.0001%
Iron   0.00005%
Titratable Acid   0.0006meq/g
RT 16790 Hydrogen Peroxide, 30% msds 100 ml 70.00 Add to Cart
RT 16792 Hydrogen Peroxide, 30% msds 450 ml 137.00 Add to Cart
0-4°C 16793 Hydrogen Peroxide, 3% Aqueous msds 500 ml 48.00 Add to Cart

arrow11Hydroquinone Aqueous Solution

0-4°C 16795-1 Hydroquinone, 1% msds 500 ml 48.00 Add to Cart
0-4°C 16795-2 Hydroquinone, 2% msds 500 ml 48.00 Add to Cart
0-4°C 16795-4 Hydroquinone, 4% msds 500 ml 54.00 Add to Cart

msdsarrow11Isopropyl Alcohol, Reagent, A.C.S.

(2-Propanol)
CH3CH(OH)CH3   F.W. 60.10   CAS #67-63-0

A.C.S. Specifications:

Color (APHA) . 10
Density @ 25°C . 0.781-0.783
Boiling Range (including 82.3°C) . 1°C
Titratable Acid or Base . 0.0001 meq/g
Residue after Evaporation . 0.001%
Solubility in water . To pass test
Water . 0.2%
RT 16930 Isopropyl Alcohol, Reagent, A.C.S. 1 qt 12.00 Add to Cart
RT 16931 Isopropyl Alcohol, Reagent, A.C.S. 4x1 qt 42.00 Add to Cart

msdsarrow11McDowell Trumps Fixative

RT 18030-05 McDowell Trumps Fixative 500 ml 32.00 Add to Cart
RT 18030-10 McDowell Trumps Fixative 1 L 38.00 Add to Cart

msdsarrow11Methanol, Reagent, A.C.S.

(Methyl Alcohol)
CH3OH   F.W. 32.04   CAS #67-56-1

Assay:   99.8%
Specific Gravity :   0.79

A.C.S. Specifications:

Residue after Evaporation . 0.001%
Boiling Range (Boiling point 64.6°C) . 2°C max
Appearance . Clear
Color (A.P.H.A.) . 10 max
Acetone & Aldehydes (0.001%) . To pass test
Titratable Acid . 0.0003 meq/g
Titratable Base . 0.0002 meq/g
Water . 0.2% max
RT 18510 Methanol, Reagent, A.C.S. 1 qt 15.00 Add to Cart
RT 18511 Methanol, Reagent, A.C.S. 4x1 qt 54.00 Add to Cart

msdsarrow11Michel's Buffer

RT 18041-05 Michel's Buffer 500 ml 21.00 Add to Cart
RT 18041-10 Michel's Buffer 1 L 28.00 Add to Cart

msdsarrow11Michel's Fixative, pH 7.0 – 7.4

RT 18040-05 Michel's Fixative 500 ml 49.00 Add to Cart
RT 18040-10 Michel's Fixative 1 L 84.00 Add to Cart
RT 18040-20 Michel's Fixative Pre-filled 20x10 ml 74.00 Add to Cart

Technical Tip:

Preparation of Tissue Biopsy in Michel's Fixative.

msdsarrow11Millonig's Phosphate Buffer

Millonig (1964); Karlsson and Schultz (1965)
Ingredients:
Sodium Phosphate, monobasic (NaH2PO4xH2O)
Sodium Phosphate, dibasic (Na2HPO4xH2O)
0.5% Sodium Chloride (NaCl)
Shipping Conditions: Ambient Temperature

The pH of this buffer is 7.4 (0.1M); the osmolarity is 440 mosmols, so this buffer is hypertonic to most body fluids. Millonig recommended to use this buffer for the fixation of very hydrated tissues with osmium tetroxide. For marine organisms, a higher concentration of sodium chloride (3%) is recommended. We supply it in a double strength (0.2M), pH 7.4. Dilution is needed before use.

0-5°C 11582-05 Millonig's Phosphate Buffer 0.2M 500 ml 21.00 Add to Cart
0-5°C 11582-10 Millonig's Phosphate Buffer 0.2M 1 L 30.00 Add to Cart

arrow11Periodic Acid, Reagent

H5IO6   F.W. 227.96   CAS #10450-60-9

Assay   >99.0%
Heavy Metals   .0.005%
Insoluble Matters   0.01%
Iron   .0.003%
Other Halogen (Cl)   0.01%
Residue after Ignition   0.01%
Sulfate   0.01%
  • Ainsworth, S.K. et al. (1972). Alkaline bismuth reagent for high resolution ultrastructural demonstration of periodic-reactive sites. J. Histochem. Cytochem., 20:995.
  • Mowry, R.W. (1958). Improved procedure for the staining of acid polysaccharides by Muller's colloidal (hydrous) ferric oxide and its combination with the Feulgen and the periodic acid Schiff reaction. Lab. Invest., 7:566.
  • Mowry, R.W. (1959). Effect of Periodic Acid used prior to Chromic Acid on the staining of Polysaccharides by Gomori's methenamine silver. J. Histochem. Cytochem., 7:288.
  • Tsuchiya, A. and Ogawa, K. (1973). Ultracytochemistry of the Periodic Acid (PA) – Phosphotungstic Acid (PTA) reaction. J. Electr. Microsc., 22:290.
  • Hanker, J.S. et al (1964). Osmiophilic reagents: new cytochemical principles for light and electron microscopy. Science New York, 146:1039.
  • Derenzini, M. et al (1986). An improved periodic acid-thiosemicarbazide-osmium technique to reveal glycoconjugates at the molecular level in-situ. J. Histochem. Cytochem., 34:1161.
RT 19325 Periodic Acid, Reagent msds 25g 32.00 Add to Cart

arrow11Periodic Acid Aqueous Solutions

  19324-05 Periodic Acid, 0.5% Aqueous msds 500 ml 25.00 Add to Cart
  19324-10 Periodic Acid, 1% Aqueous msds 500 ml 28.00 Add to Cart
  19324-50 Periodic Acid, 5% Aqueous msds 500 ml 38.00 Add to Cart

arrow11Phosphate Buffer, 0.1M*

Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only.

  19340-60 Phosphate Buffer, pH 6.0 msds 1 L 38.00 Add to Cart
  19340-65 Phosphate Buffer, pH 6.5 msds 1 L 38.00 Add to Cart
  19340-70 Phosphate Buffer, pH 7.0 msds 1 L 38.00 Add to Cart
  19340-72 Phosphate Buffer, pH 7.2 msds 1 L 38.00 Add to Cart

*Meant for biological research only. Not to be used for medical or any other purposes.

arrow11Phosphate Buffer Saline (PBS)

PBS is commonly used in biochemistry. It is a salty solution containing calcium chloride, sodium phosphate and potassium phosphate. PBS is isotonic and non-toxic to cells. 1X PBS final concentration is 0.137M NaCl, 0.01M Na2HPO4, 0.0027M KCl and pH 7.4.

RT 19342-10 Phosphate Buffered Saline, 10X msds 1 L 29.00 Add to Cart
RT 19242-40 Phosphate Buffered Saline, 10X 1 gal 96.00 Add to Cart
RT 19343-10 Phosphate Buffered Saline, 2X msds 1 L 26.00 Add to Cart
RT 19343-40 Phosphate Buffered Saline, 2X 1 gal 86.00 Add to Cart
RT 19344-10 Phosphate Buffered Saline, 1X msds 1 L 22.00 Add to Cart
RT 19344-20 Phosphate Buffered Saline, 1X 1 gal 74.00 Add to Cart

For a complete line of all of our Salt Buffers, click here

msdsarrow11Phosphomolybdic Acid

Crystal, Reagent, A.C.S.

(dodeca-Molybdophosphoric Acid)

H3PO4·12(MoO3)·24H2O   F.W. 2257.6   CAS #51429-74-4

RT 19400 Phosphomolybdic Acid 100g 54.00 Add to Cart

arrow11Phosphomolybdic Acid Aqueous Solutions

RT 19402-1 Phosphomolybdic Acid, 1% Aqueous msds 500 ml 36.00 Add to Cart
RT 19402-10 Phosphomolybdic Acid, 10% Aqueous msds 500 ml 104.00 Add to Cart

arrow11Phosphotungstic Acid, Crystal, Reagent, Highest Purity

(Tungstophosphoric Acid)

H3[P(W3O10)4]   aq F.W. 2880.17+aq   CAS #12501-23-4

Specifications:

Solubility   Practically clear, colorless solution.
Ammonia (NH )   0.004%
Chloride (Cl)   0.03%
Insoluble Matter   0.02%
Nitrate (NO3 )   0.004%
Sulfate (SO4 )   0.005%
Heavy Metals and Iron.   No color formation onaddition of Hydrogen Sulfide

Used as a Fixative:

Issidorides, M.R., and Kasorchis, T. (1981).
Dispersed and compact chromatin demonstrated with a new EM Method: phosphotungstic acid-hematoxylin block-staining. Histochemistry 73,21.

Used widely as a Negative Stain:

  • As Spray method: Horne, R.W., and Pasquali-Ronchette, I. (1974). A negative staining-carbon film technique for studying viruses in the electron microscope.
  • Preparation procedures for examining icosahedral and filamentous viruses. J. Ultrastruct. Res. 47, 361. Locke, M., and Kirhman, N. (1971).
  • Hot Alcoholic phosphotungstic acid and uranyl acetate as routine stains for thick and thin sections. J. Cell Biol., 50, 550., Farragiana, T., and Marinozzi, V. (1979).
  • Phosphotungstic acid staining of polysaccharides containing structures on epoxy embedded tissues. J. Submicrosc. Cytol. 11, 263.Bloom, E.E., And Aghajanian, G.K. (1968).
  • Fine structural and cytochemical analysis of the staining of synaptic junctions with PTA. J. Ultrastruct. Res. 22, 261.
RT 19500 Phosphotungstic Acid, Crystal, Reagent, Highest Purity msds 100g 59.50 Add to Cart

arrow11Phosphotungstic Acid Aqueous Solutions

RT 19502-1 Phosphotungstic Acid, 1% Aqueous msds 500 ml 34.00 Add to Cart
RT 19502-3 Phosphotungstic Acid, 3% Aqueous msds 500 ml 46.00 Add to Cart
RT 19502-5 Phosphotungstic Acid, 5% Aqueous msds 500 ml 63.00 Add to Cart

arrow11 Picric Acid, Reagent, Crystal, A.C.S.

(2,4,6-Trinitrophenol)

C6H2(NO2) 3OH   FW 229.11   CAS #88-89-1

A.C.S. Specifications:

Water   min 30%
Melting Point (dried)   121-123°C
Insoluble in Resinous Matter.   0.01%
Insoluble in benzene   0.1%
Sulfate   0.01%
RT 19550 Picric Acid, Reagent, Crystal, A.C.S. msds 100g 94.00 Add to Cart

arrow12Picric Acid Solutions

RT 19552 Picric Acid Saturated, Aqueous msds 500 ml 45.00 Add to Cart
RT 19553 Picric Acid, 0.1% in Acetone msds 500 ml 50.00 Add to Cart
RT 19554 Picric Acid, 4.67% in 95% Alcohol msds 500 ml 50.00 Add to Cart

arrow11Poly-L-Lysine Solutions

msdsarrow11A/ Poly-L-Lysine Hydrobromide 30,000-70,000

CAS #25988-63-0

A polycation, which binds to DNA, red cell membranes and any negatively, charged proteins. It is useful for promoting cell adhesion; for the preparation of polycationic beads, useful in immobilization techniques; immobilization of membranes; immobilization of plant protoplasts. In cell culture, immobilization by micro encapsulation with the alginate-poly-L-lysine microcapsule system.

We offer a prepared 0.1% aqueous solution, micro-filtered, which is ideal for cell culture growth.

RT 19320-A Poly-L-Lysine, 0.1% Solution 10 ml 28.50 Add to Cart
RT 19321-A Poly-L-Lysine, 0.1% Aqueous Solution 100 ml 280.00 Add to Cart
msdsarrow11B/ Poly-L-Lysine Hydrobromide 70,000-150,000

CAS #25988-63-0

Poly-L-lysine with a molecular weight of >70,000. This solution is useful in promoting cell adhesion to solid substrates. Used for nuclear antigen-coated red cells in hymolytic plaque assay.

We offer a prepared 0.1% aqueous solution which is micro-filtered and is used to increase tissue adhesion to the glass slide, which is needed for immunohistochemical and immunolabeling techniques, as well as lengthy staining procedures.

RT 19320-B Poly-L-Lysine, 0.1% Solution 10 ml 14.00 Add to Cart
RT 19321-B Poly-L-Lysine, 0.1% Aqueous Solution 100 ml 118.00 Add to Cart

msdsarrow12Potassium Dichromate, Crystal, Reagent, A.C.S.

K2Cr2O7   F.W. 294.18   CAS #7778-50-9

A.C.S. Specifications:

Insoluble and NH4OH ppt. 0.005%
Chloride 0.001%
Sulfate 0.005%
Calcium 0.003%
Sodium 0.02%
Loss on drying at 105°C 0.05%
RT 20100 Potassium Dichromate, Crystal, Reagent, A.C.S. 250g 29.50 Add to Cart

msdsarrow12Potassium Dichromate Aqueous Solution

RT 20103-3 Potassium Dichromate, 3% Aqueous msds 500 ml 30.00 Add to Cart
RT 20103-4 Potassium Dichromate, 5% Aqueous msds 500 ml 37.00 Add to Cart

arrow12Potassium Ferricyanide, Reagent, A.C.S.

K3Fe(CN)6   F.W. 329.25   CAS #13746-66-2

A.C.S. Specifications:

Chloride 0.01%
Ferro Compounds (Fe(Cn)4) 0.05%
Insoluble Matters. 0.005%
Sulfate 0.01%
RT 20150 Potassium Ferricyanide msds 100g 22.00 Add to Cart
RT 25102-20 Potassium Ferricyanide, 20% Aqueous msds 500 ml 39.00 Add to Cart

arrow12Potassium Ferrocyanide Aqueous Solution

RT 25154-2 Potassium Ferrocyanide, 2% Aqueous msds 500 ml 22.00 Add to Cart
RT 25154-5 Potassium Ferrocyanide, 5% Aqueous msds 500 ml 30.00 Add to Cart
RT 25154-10 Potassium Ferrocyanide, 10% Aqueous msds 500 ml 25.00 Add to Cart
RT 25154-20 Potassium Ferrocyanide, 20% Aqueous msds 500 ml 32.00 Add to Cart

msdsarrow12Potassium Hydroxide, Pellets, Reagent, A.C.S.

KOH   F.W. 56.11   CAS #1310-58-3

Assay   >85.0%
Water contains:   10-15%
RT 20155 Potassium Hydroxide, Pellets, Reagent, A.C.S. 100g 10.00 Add to Cart

arrow12Potassium Hydroxide Aqueous Solutions

RT 20156-1 Potassium Hydroxide, 1% Aqueous   500 ml 24.00 Add to Cart
RT 20156-2 Potassium Hydroxide, 4% Aqueous msds 500 ml 31.00 Add to Cart
RT 20156-10 Potassium Hydroxide, 10% Aqueous msds 500 ml 25.00 Add to Cart

msdsarrow12Potassium Iodide, Granular, Reagent, A.C.S.

KI F.W. 166.00 CAS #7681-11-0

Refer to Bismuth Carbonate for references on use.

RT 20158 Potassium Iodide 50g 48.00 Add to Cart
RT 25109-3 Potassium Iodide, 3% Aqueous 500 ml 19.00 Add to Cart

msdsarrow12Potassium Permanganate, Reagent, A.C.S.

KMnO4   F.W. 158.03   CAS #7722-64-7

A.C.S. Specifications:

Chloride & Chlorate 0.005%
Nitrogen Compounds 0.005%
Sulfate .0.02%
Insoluble Matters 0.2%

An EM fixative and a metal stain.

J. Ultrastruct. Res., 21,424 (1968)

RT 20200 Potassium Permanganate 250g 36.00 Add to Cart

arrow12Potassium Permanganate, Aqueous Solutions

RT 20202 Potassium Permanganate, 0.25% Aqueous   500 ml 23.00 Add to Cart
RT 20203 Potassium Permanganate, 0.3% Aqueous msds 500 ml 24.00 Add to Cart
RT 20204 Potassium Permanganate, 1% Aqueous   500 ml 28.00 Add to Cart

msdsarrow12Propylene Glycol, Reagent, A.C.S.

(1,2-Propanediol)
CH3CHOHCH2OH   F.W. 76.10   CAS #57-55-6

Specific Gravity   (H2O=1): 1.04
Assay   >99.5%

Specifications:

Residue after Ignition 0.005%
Chloride 1ppm
Water 0.2%
Titratable Acid 0.0005meq/g
RT 20350 Propylene Glycol, Reagent, A.C.S. 450 ml 27.00 Add to Cart
RT 20352 Propylene Glycol, Reagent, A.C.S. 4x450 ml 94.00 Add to Cart

Rosin Solution

RT 20550-05 Rosin Alcohol Stock Solution 500 ml 36.00 Add to Cart
RT 20550-15 Rosin Alcohol Working Solution 500 ml 33.00 Add to Cart

msdsScott's Tap Water Substitute

Magnesium sulfate buffered with sodium bicarbonate. Ready-to-use.

RT 26070-06 Scott's Tap Water Substitute 1 L 31.00 Add to Cart
RT 26070-07 Scott's Tap Water Substitute 4 L 96.00 Add to Cart

msdsAlcoholic Stain SolutionsSchiff's Reagent

For use in the Periodic Acid Leucofuchsin (PAS) method as a general tissue stain.

0-5°C 26052-05 Schiff's Reagent 500 ml 43.00 Add to Cart
0-5°C 26052-06 Schiff's Reagent 1 L 70.00 Add to Cart

msdsSilver Nitrate, Reagent, A.C.S.

AgNO3   F.W. 169.89  CAS #7761-88-8

Assay   99.9+%

A.C.S. Specifications:

Substances not precipitated by HCl-max 0.010%
Sulfate max 0.0002%
Copper max 0.0002%
Iron max 0.0002%
Lead max 0.001%

References for Silver stain:

  • Elektronemikrokopische Swift, J.A. (1968). The electron histochemistry of cystine-containing proteins in thin transverse sections of human hair. J.R. Microsc. Soc. 88,449.
  • Elektronemikrokopische Thiery, J.P. (1967). Mise en evidence des Polysaccharides sur coupes fines en microscopie eletronique. J. Microsc. (Paris) 6,987.
  • Elektronemikrokopische Rambourg, A. (1967). An improved silver methenamine technique for the detection of periodic acidreactive complex carbohydrates with the electron microscope.J. Histochem. Cytochem. 15, 409.
  • Elektronemikrokopische Hermandez et al. (1968). Periodic acid-chromic acid-methenamine silver technique for glycoprotein detection in the electron microscope. J. Histochem. Cystochem. 16, 507.
  • Elektronemikrokopische Ribi, W.A. (1976). A golgi-electron microscope method for insect nervous tissue. Stain Technol. 51,13.
  • Elektronemikrokopische Braak, H., and Braak, E. (1982). A simple procedure for electron microscopy of Golgi-impregnated nerve cells. Neurosci. Lett. 32,1.
RT 21050 Silver Nitrate, Reagent, A.C.S. 25g 70.00 Add to Cart
RT 21052 Silver Nitrate, Reagent, A.C.S. 100g 270.00 Add to Cart

Silver Nitrate Aqueous Solutions

RT 21054-1 Silver Nitrate, 1%, Aqueous msds 500 ml 60.00 Add to Cart
RT 21054-2 Silver Nitrate, 2%, Aqueous msds 500 ml 66.00 Add to Cart
RT 21054-5 Silver Nitrate, 5%, Aqueous msds 500 ml 133.00 Add to Cart
RT 21054-10 Silver Nitrate, 10%, Aqueous msds 500 ml 222.00 Add to Cart
RT 21054-20 Silver Nitrate, 20%, Aqueous msds 500 ml 350.00 Add to Cart

Sodium Bicarbonate, Powder, Reagent, A.C.S.

NaHCO3   F.W. 84.01   CAS #144-55-8

Assay   97.7-100.3%

Specifications:

Insoluble Matters .0.015%
Chloride 0.003%
Phosphate 0.001%
Sulfur Compounds 0.003%
Ammonium 5ppm
Iron 0.001%
Potassium 0.05%
RT 21125 Sodium Bicarbonate msds 500g 21.00 Add to Cart
RT 21127 Sodium Bicarbonate, 5% Aqueous msds 500 ml 16.00 Add to Cart

arrow11Sodium Cacodylate Buffer

Prepared from Sodium Cacodylate Trihydrate (F.W. 214.02)

0-5°C 11650 Sodium Cacodylate Buffer msds 225 ml 28.00 Add to Cart
0-5°C 11652 Sodium Cacodylate Buffer   500 ml 40.00 Add to Cart
0-5°C 11653 Sodium Cacodylate Buffer, 0.2M, pH 7.4   1 L 82.00 Add to Cart
0-5°C 11654 Sodium Cacodylate Buffer, 0.4M, pH 7.2 msds 500 ml 58.00 Add to Cart
0-5°C 11655 Sodium Cacodylate Buffer, 0.4M, pH 7.2   1 L 108.00 Add to Cart

Sodium Hydroxide, Solution

CAS #1310-73-2

Carbonate-free. This solution is prepared from A.C.S. Reagent grade sodium hydroxide, and is standardized colormetrically against potassium biphthalate primary reference material.

Normal Concentration 1.98-2.02

RT 21170 Sodium Hydroxide Solution, 2N msds 225 ml 13.00 Add to Cart
RT 21170-01 Sodium Hydroxide Solution, 1N msds 225 ml 12.00 Add to Cart

Sodium Hydroxide Aqueous Solutions

RT 21172-1 Sodium Hydroxide, 1% Aqueous msds 500 ml 19.00 Add to Cart
RT 21172-2 Sodium Hydroxide, 2.5% Aqueous msds 500 ml 29.00 Add to Cart
RT 21172-3 Sodium Hydroxide, 3.1% Aqueous msds 500 ml 29.00 Add to Cart
RT 21172-10 Sodium Hydroxide, 10% Aqueous msds 500 ml 33.00 Add to Cart

msdsSodium Bisulfite Solution

A 1% aqueous stock solution for fungal staining.

RT 26084-05 Sodium Bisulfite Solution 500 ml 19.00 Add to Cart
RT 26084-06 Sodium Bisulfite Solution 1 L 33.00 Add to Cart

Sodium Meta-Bisulfate Aqueous Solution

RT 21175-1 Sodium Meta-Bisulfite, 1% Aqueous   500 ml 29.00 Add to Cart
RT 21175-3 Sodium Meta-Bisulfite, 3% Aqueous msds 500 ml 30.00 Add to Cart
RT 21175-5 Sodium Meta-Bisulfite, 5% Aqueous msds 500 ml 31.00 Add to Cart

Sodium Thiosulfate Aqueous Solution

RT 21360-1 Sodium Thiosulfate, 1% Aqueous msds 500 ml 25.00 Add to Cart
RT 21360-2 Sodium Thiosulfate, 2% Aqueous msds 500 ml 32.00 Add to Cart
RT 21360-10 Sodium Thiosulfate, 10% Aqueous msds 500 ml 27.00 Add to Cart

msdsmsdsarrow11Sorensen's Phosphate Buffer

A Phosphate buffered mixed salt prepared from sodium phosphate monobasic and sodium phosphate dibasic.

0-5°C 11600-10 Sorensens Phosphate Buffer, 0.2M, pH 7.2 msds 1 L 30.00 Add to Cart
0-5°C 11600-40 Sorensens Phosphate Buffer, 0.2M, pH 7.2 4 L 106.00 Add to Cart
0-5°C 11601-10 Sorensens Phosphate Buffer, 0.2M, pH 7.4 msds 1 L 30.00 Add to Cart
0-5°C 11601-40 Sorensens Phosphate Buffer, 0.2M, pH 7.4 4 L 106.00 Add to Cart

msdsSucrose, Reagent, A.C.S.

C12H22O11   F.W. 342.20   CAS #57-50-1

Specifications:

Insoluble Matters 0.005%
Loss on Drying 105°C 0.03%
Residue after Ignition 0.01%
Titratable acid 0.0008meq/g
Chloride 0.005%
Sulfate 0.005%
Heavy Metals 5ppm
Iron 5ppm
Invert Sugar 0.05%
RT 21600 Sucrose, Reagent, A.C.S. 500g 22.00 Add to Cart

msdsAlcoholic Stain Solutionsarrow11Tannic Acid, Reagent, A.C.S., EM Grade

C76H52O46   F.W. 1701.28   CAS #1401-55-4

RT 21700 Tannic Acid, Reagent, A.C.S., EM Grade 100g 13.00 Add to Cart

arrow11Tannic Acid Aqueous Solutions

  21702-5 Tannic Acid, 5% Aqueous msds 500 ml 26.00 Add to Cart
  21702-10 Tannic Acid, 10% Aqueous msds 500 ml 30.00 Add to Cart

arrow11Tartrazine Solutions

RT 21740 Tartrazine, 1.5% in 1.5% Acetic Acid, Aqueous msds 500 ml 26.00 Add to Cart
RT 21741 Tartrazine, 1.5% Aqueous   500 ml 26.00 Add to Cart
RT 21742 Tartrazine, Saturated in Cellosolve msds 500 ml 63.00 Add to Cart

arrow11Thionin Solutions

RT 21910 Thionin, 0.5% in 20% Alcohol   500 ml 50.00 Add to Cart
RT 21911 Thionin, 0.1% Aqueous msds 500 ml 43.00 Add to Cart

tissue-tek vip fixativemsdsarrow10 Tissue-Tek® VIP® Fixative

Eliminate the need for routine warm water flushing with the Tissue-Tek® VIP® Fixative. Using the unique Neutra-pHase™ technology, the Tissue-Tek® VIP® Fixative produces no precipitates in the presence of alcohol, contrary to all other 10% neutral buffered formalin products. The Tissue-Tek® VIP® Fixative will not promote formation of salts within the tissue processor lines which can cause costly damages to any brands of tissues processors unless regular maintenance is performed. Since the base ingredient is formalin, it is fully compatible and can replace 10% NBF without any adjustments to your procedures. The product is offered in three convenient package sizes: 1 gallon, 2.5 gallons and 5 gallons.

  62582-01 Tissue-Tek® VIP® Fixative 1 gal 41.00 Add to Cart
  62582-04 Tissue-Tek® VIP® Fixative 4x1 gal 142.00 Add to Cart
  62582-02 Tissue-Tek® VIP® Fixative 2.5 gal 97.00 Add to Cart
  62582-05 Tissue-Tek® VIP® Fixative 5 gal 150.00 Add to Cart
  62582-50 Spigot for 62582-02 & 62582-05 each 10.00 Add to Cart

CytoFixCytoFixarrow11CytoFix

Cytology fixative spray designed for slide based cytology specimens for cellular preservation and transport.

Quickly spray a fine mist over the slide (covering the entire specimen) for cellular fixation and reduce air drying of the material.

CytoFix forms a thin, tough, clear protective coating over the specimen with a water and alcohol solution of Polyethylene glycol for cellular fixation and does not require rehydration by the laboratory prior to staining. Allows safe storing or transporting of slides.

Allow specimens to dry before sending to lab or for storage.

  • Dries in 4 to 6 minutes
  • Water soluble
  • Won't cloud or obscure specimen
  • Contains no chlorofluorocarbons

Available in pump bottle or aerosol can.

22805-01 CytoFix (Non-Aerosol) 100 ml 14.50 Add to Cart
22805-02 CytoFix Can 4 oz 14.50 Add to Cart

msdsarrowToluene Reagent, A.C.S.

C6H5CH3   FW 92.14   CAS #108-88-3
Specific Gravity (H2O=1): 0.866
b.p. 110-1110C
Assay. 99.9%

Specifications:

Color (A.P.H.A.) 10
Residue after evaporation 0.001%
Subs. darkened by H2SO4 To pass test
Sulfur compound (as S) 0.003%
Water 0.03%
RT 22030 Toluene Reagent, A.C.S. 450 ml 10.50 Add to Cart
RT 22032 Toluene Reagent, A.C.S. 4x450 ml 38.00 Add to Cart

msdsarrowTris (Hydroxymethyl) Aminomethane, Free-base, Reagent Grade

White crystalline powder
C4H11NO3   F.W. 121.14   CAS #77-86-1
A 40% (w/v) aqueous solution. It is clear and colorless.
Assay >99.9%
Water .<0.2%
Heavy Metals.-2ppm

RT 11700 Tris (Hydroxymethyl) Aminomethane, Free-base, Reagent Grade 100g 17.00 Add to Cart
RT 11720 Tris (Hydroxymethyl) Aminomethane, Free-base, Reagent Grade 500g 49.00 Add to Cart

msdsarrowTris, (hydroxymethyl) aminomethane maleate

Tris Maleate Buffer
0.2M pH range 6.4-8.0

0-5°C 11730 Tris Maleate Buffer 250 ml 21.00 Add to Cart
0-5°C 11740 Tris Maleate Buffer 500 ml 28.00 Add to Cart

msdsarrowWater Deionized, Reagent Grade A.C.S.

H2O F.W. 18.02 CAS #7732-18-5
EMS Reagent Grade Water is typically prepared at 18 megohm/cm specific resistance using a reverse osmosis, mixed deionization, activated filtration and final filtration at 0.2 microns.
Color (APHA) <+/-5
Bacteriological purity 0 CFUs/L
Residue after evaporation 10ppm
Coliform negative

  22800-01 Deionized Water 1 gal 10.00 Add to Cart
  22800-05 Deionized Water 5 gal 42.00 Add to Cart

msdsarrowXylenes, Reagent, A.C.S.

(Xylol, Dimethylbenzene)
C6H4(CH3)2   FW 106.27   CAS #1330-20-7
Specific Gravity (H2O=1): 0.865
b.p. 136-140°C
This reagent is a mixture of isomers (ortho, meta, and para) and may contain Ethylbenzene.

Specifications:

Color (A.P.H.A.) 10
Subs. darkened by H2SO4 To pass test
Sulfur Compounds (as S) 0.003%
Water 0.05%
Residue after evaporation 0.002%
RT 23400 Xylenes, Reagent, A.C.S. 450 ml 8.00 Add to Cart

msdsarrow11Xylene: Peanut oil, 2:1

RT 23402 Xylene: Peanut oil, 2:1 500 ml 43.00 Add to Cart

msdsarrow11Xylene Substitute

A xylene substitute for use in histology and cytology. It is safer and a more preferable alternative to xylene. It can be used as a solvent and clearing agent, as well as for dissolving paraffin waxes, glues and adhesives.

Properties:

  • Minimal tissue shrinkage.
  • Dries slowly with no residue.
  • Less toxic than xylene.
  • Soluble with alcohol, paraffin embedding media, and mounting media.

It can be used in all procedures that require xylene. Biodegradable.

RT 23410-01 Xylene Substitute 1 gal 102.00 Add to Cart
RT 23410-04 Xylene Substitute 4x1 gal 375.00 Add to Cart
RT 23410-05 Xylene Substitute 5 gal 390.00 Add to Cart

msds23405-01arrow11Histosol™ – Xylene Substitute

Histosol™ is the original high flash point (114°F TCC) histological clearing agent. It is intended to be used as a replacement for xylene where the hazards associated with aromatic hydrocarbon vapors are to be reduced. Museum-quality tissue slides can be prepared with Hitosol without change in protocol or procedure. Histosol is manufactured from petrochemical products and is miscible in all proportions with ethanol, isopropanol, and t-butanol. It is also miscible with all paraffin-based tissue embedding media and all permanent mounting materials.

RT 23405-01 Histosol™ – Xylene Substitute 1 gal 59.00 Add to Cart
RT 23405-04 Histosol™ – Xylene Substitute 4x1 gal 216.00 Add to Cart

msdsAlcoholic Stain Solutionsarrow11Xylene Substitute – Hemo-De®*

An intensive study from 1992 throughout 2000 has shown that Hemo-De® (d-Limonene) a terpene-based chemical with a pleasant citrus fragrance, was able to replace many of the clinical laboratories toxic reagents, such as carbol-xylene, xylene, ethyl acetate and formalin.

In Parasitology – Hemo-De replaces ethyl acetate in the concentration procedure; Hemo-De replaces carbol-xylene, xylene and formalin in the trichrome procedure.

In Histology – Hemo-De replaces xylene in most all procedures. Hem-De is a superior solvent and Clearing agent that can be used in all tissue processing, deparaffiniating and slide preparation.

Hemo-De Features:

  • Soluble with alcohol and mounting media
  • Biodegradable, non-corrosive, non-flammable (Combustible)
  • Contains no benzene and no toluene
  • Low toxicity levels
  • Minimal tissue shrinkage
  • Reasonably fast drying and leaves no residue

Hemo-De Application:

In Research and Medical – Hematology, Cytology, Pathology, Microbiology, Histology, Pathology, Hospital.

In Mechanical Applications – Cleaning of machine parts, cleaning tools, engines etc.

In Electronic Applications – Circuits boards, electronic components, tools.

General Applications – Clean metal and glass surfaces. Dilute with water can be used to clean all plastic surfaces. Removal of adhesives, glues, tars…

Hem-De Information:

Chemical name: d-Limonene (Terpene Hydrocarbon, n.o.s.)
Composition: d-Limonene, CAS [5989-27-5]98.0%
Butylated Hydroxyanisolve (BHA), CAS
[25013-16-5]…….<0.024%
Flash Point (TCC): 121°F (49.4°C)
Boiling Point: 349°F (176°C) @ 760mmHg
Freezing Point: -74°C
Shipping: Terpene Hydrocarbons, n.o.s. ID No.
04858003, Flammable Liquid, Group III

References:

  1. W.E. Aldeen and D. Hale. "Use of Hem-De to eliminate toxic agents used for concentration and trichrome staining of intestinal parasites" J. of Clinical Microbiology, July 1992, 1893-1895, Vol. 30, No. 7.
  2. Metzger, Zvi DMD et al. "Gutta-Percha Softening: 'Hem-De' as a Xylene Substitute" J. of Endodontics". 26(7):385-388, July 2000.
  3. Devon C. Hale, MD; et al. "Use of a Single Slide Trichrome-Stained Concentrate for the detection of intestinal Parasites Stained Concentration Procedure for Ova and Parasites" American Journal of Clinical Pathology, Vol. 106, N0. 2. Aug. 1996.
  4. J. M. Miller et al. "Biodegradable, effective substitute for xylene in the Ehrlich indole procedure". J. of Clinical Microbiology, Aug. 1994, 2028-2030, Vol 32, No 8.
RT 23412-01 Xylene Substitute – Hemo-De® 1 gal 110.00 Add to Cart
RT 23412-04 Xylene Substitute – Hemo-De® 4x1 gal 399.00 Add to Cart
RT 23412-05 Xylene Substitute – Hemo-De® 5 gal 450.00 Add to Cart

*Hemo-De® is a product of Scientific Solvents.

msdsarrow11Russell's Zenker Fixative (Russel's Modified Zenker)

Due to the hazardous nature of mercury, Zenker's fluid is no longer available. In place we are offering Russell's Zenker Fixative, which contains Zinc Chloride. The staining results produced by this fixative are comparable to those of Zenker's Stock solution. Russell's Modified Zenker's is used in the same manner as Zenker's stock solution.

RT 64123-05 Russell's Zenker Fixative 500 ml 26.00 Add to Cart
RT 64123-06 Russell's Zenker Fixative 1 L 43.00 Add to Cart

arrow11Zinc/Formalin

See Formaldehyde/Zinc

Certified Dyes/Stains arrow13arrow13