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Electron Microscopy Sciences

Histology and Light Microscopy

arrow13IHC Antigen Retrievers

EMS is proud to introduce a broad selection of Antigen Retrievers in IHC...

msdsmsdsarrow11I. Trypsin Reagent

Description: Trypsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin; mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Trypsin digestion improves immunoreactivity of some antigens in FFPE tissue sections. For cytokeratin clone AE3 and AE1/AE3, this enzyme works much better than boiling the tissue with citrate buffer.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: This enzyme is supplied as concentrated form along with buffer. The kit comes in 2 sizes. Small: Reagent B (Buffer) is 15 ml and the Reagent E (Enzyme) is 2 ml.
Large: Reagent B (Buffer) is 100 ml and the Reagent E (Enzyme) is 10 ml.
Cat. # Description Qty. Price  
64142-01 Trypsin Kit Small kit 75.00 Add to Cart
64142-02 Trypsin Kit Large kit 145.00 Add to Cart

msdsarrow11 II. Pronase Reagent, Ready To Use

Description: Pronase is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pronase digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: Ready-to-use Pronase reagent available in 2 sizes: 15 and 100 ml.
Cat. # Description Qty. Price  
64142-03 Pronase Reagent 15 ml 75.00 Add to Cart
64142-04 Pronase Reagent 100 ml 145.00 Add to Cart

msdsarrow11 III. Pepsin Reagent, Ready To Use

Description: Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. Pepsin digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: Ready-to-use Pepsin reagent available in 2 sizes: 15 and 100 ml.
Cat. # Description Qty. Price  
64142-05 Pepsin Reagent 15 ml 75.00 Add to Cart
64142-06 Pepsin Reagent 100 ml 145.00 Add to Cart

msdsarrow11 IV. Citrate Buffer pH 6.0 (10x)

Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 6 antigen retriever solution.
Preparation of Working Solution:
Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.
Cat. # Description Qty. Price  
64142-07 Citrate Buffer pH 6.0 100 ml 75.00 Add to Cart
64142-08 Citrate Buffer pH 6.0 1000 ml 145.00 Add to Cart

msdsarrow11 V. EDTA Buffer pH 8.5 (10x)

Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 8.5 antigen retriever solution.
Preparation of Working Solution: Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.
Cat. # Description Qty. Price  
64142-09 EDTA Buffer pH 8.5 100 ml 75.00 Add to Cart
64142-10 EDTA Buffer pH 8.5 1000 ml 145.00 Add to Cart

msdsarrow11 VI. Tris Buffer pH 10.0 (10x)

Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. The use of this buffer on FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: 1x buffer solution is intended for heat-induced antigen retriever in IHC. Please refer to primary antibody protocol.
Reagent: 10x, pH 10 antigen retriever solution.
Preparation of Working Solution:
Dilute this 10x buffer as needed (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, 1x buffer can be stored at 2-8°C.
Cat. # Description Qty. Price  
64142-11 Tris Buffer pH 10.0 100 ml 75.00 Add to Cart
64142-12 Tris Buffer pH 10.0 1000 ml 145.00 Add to Cart

arrow11 VII. ImmunoHistoZyme™, Ready-to-Use

Description: ImmunoHistoZyme is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In IHC most commonly used fixative like formalin mask tissue antigens (cellular, membrane and nuclear) by cross-linking process, this results in poor or no staining in IHC. ImmunoHistoZyme digestion of FFPE tissue section improves accessibility of antibodies to tissue antigens.
Storage: 2-8°C. DO NOT FREEZE
Intended Use: Use as antigen retriever for some antibodies in IHC. Please refer to primary antibody protocol for IHC.
Reagent: Ready-to-use ImmunoHistoZyme Reagent.
Cat. # Description Qty. Price  
64142-13 ImmunoHistoZyme 15 ml 75.00 Add to Cart
64142-14 ImmunoHistoZyme 100 ml 145.00 Add to Cart

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