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Electron Microscopy Sciences

Histology and Light Microscopy

arrow13Prepared Staining Kits for Histology and Pathology

H. FAT AND LIPID STAINS

Alcoholic Stain SolutionsarrowHerxheimer's Technique

To stain fat and cholesterol.

Stain results:

Nuclei:   Blue
Fat:   Orange-Red
Cholesterol:   Red

References:

  • Clark, G., Ed.: Staining Procedures; 3rd Ed.; Baltimore: Williams and Wilkins Co., c. 1973, p. 143
  • Conn's, H.J.: Biological Stains; 8th Ed., Baltimore: Williams and Wilkins Co., c. 1969, p.100
RT 26500-01 Sudan IV Staining Solution (Herxheimer II) msds 500 ml 85.00 Add to Cart
RT 26500-02 Harris' Hematoxylin msds 500 ml 34.00 Add to Cart
RT 26500-04 Acetic Acid, 1%, Aqueous msds 500 ml 18.00 Add to Cart

Alcoholic Stain SolutionsarrowFat Stains in Supersaturated Alcohol

For fat and lipid stain.

Stain results:

Fat:   Deep orange-red with Oil Red O (lighter with
Sudan IV) Orange-yellow with Sudan II
Nuclei:   Blue
Erythrocytes:   Sometimes Green
Cytoplasm:   Lighter Green

References:

  • Lillie, R.D.,Histopathologic Technical and Practical Histochemistry, 3rd edition, McGraw-Hill, NY, 1965
  • Clark, G., (ed) Staining Procedures, 3rd edition, Williams & Wilkins, Baltimore, p. 149, c. 1973
RT 26503-01 Sudan IV-Isopropanol Solution msds 500 ml 87.00 Add to Cart
RT 26503-02 Oil Red O-Isopropanol Solution msds 250 ml 16.00 Add to Cart
RT 26503-03 Sudam III-Isopropanol Solution msds 250 ml 18.00 Add to Cart
RT 26503-04 Mayer Hematoxylin msds 500 ml 40.00 Add to Cart
RT 26503-05 Erhlich's Hematoxylin, Diluted   500 ml 40.00 Add to Cart
RT 26503-06 Sodium Phosphate, 1%, Aqueous msds 500 ml 19.00 Add to Cart

arrowOil Red in Propylene Glycol Method

Fat Stain results:

Fat:   Red
Nuclei:   Blue

References:

AFIP Manual of Histologic Staining Methods, Ed. L. Luna, 3rd ed., New York, McGraw-Hill Publications, c. 1968, p. 140

  26504-01 Oil Red O Solution msds 500 ml 47.00 Add to Cart
  26504-02 Mayer's Hematoxylin  msds 500 ml 38.00 Add to Cart
  26504-2A Harris Hematoxylin   500 ml 33.00 Add to Cart
  26504-03 Propylene Glycol, 85%, Aqueous msds 500 ml 21.00 Add to Cart
  26504-04 Hydrochloric Acid, 5% msds 500 ml 17.00 Add to Cart
  26504-05 Ammonia Water, 0.3%   500 ml 13.00 Add to Cart
  20350 Propylene Glycol, Absolute msds 450 ml 27.00 Add to Cart

Technical Tip

Hematoxylin Staining Tips

Alcoholic Stain SolutionsarrowNile Blue A Stain

Lipid stain.

Stain results:

Lipofuscins:   Unstained – Natural Yellow
Melanin's:   Green
RT 26511-01 Nile Blue A Staining Solution msds 500 ml 40.00 Add to Cart
RT 26511-02 Sulfuric Acid, 1% Aqueous msds 500 ml 20.00 Add to Cart

Alcoholic Stain SolutionsarrowPearse's Method for Phospholipids

For the staining of phospholipids, early lipofuchsin, etc.

Stain results:

Phospholipids:   Blue
Nuclei and Nucleoli:   Red
Early lipofuchsin, eosinophil granules, keratin,
keratohyalin, human elastic tissues:
  Blue to Purple

References:

  • Pearse, A. G.E., J. Pathl. Bact., 70:554-557, 1995
  • Luna, L.G. (ed), Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd Ed., McGraw-Hill, c. 1968, p. 148.
RT 26516-01 Luxol Fast Blue MBS Solution, 0.1% msds 500 ml 30.00 Add to Cart
RT 26516-02 Lithium Carbonate, 0.05% Aqueous msds 500 ml 23.00 Add to Cart
RT 26516-03 Neutral Red Solution, 1%, Aqueous msds 250 ml 21.00 Add to Cart

Mineral & Pigment Prepared Stains arrow13arrow13