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Electron Microscopy Sciences

Histology and Light Microscopy

arrow13Prepared Staining Kits for Histology and Pathology

I. MINERAL AND PIGMENT STAINS

arrowRhodanine Method for Copper

Mineral and Pigment stain.

Stain results:

Copper:   Bright Red to Red Yellow
Nuclei:   Light Blue

References:

  • Sheehan and Hrapchack, Theory and Practice of Histotechnology. St. Louis, The Mosby Company 1980 p. 230.
RT 26602-01 Rhodanine Saturated Solution msds 100 ml 15.00 Add to Cart
RT 26602-02 Mayer's Hematoxylin msds 500 ml 40.00 Add to Cart
RT 26602-03 Sodium Borate, 5% Aqueous msds 500 ml 29.00 Add to Cart

msdsAlcoholic Stain SolutionsarrowJohnson's Method for Iron - (Microincineration)

Mineral and Pigment stain.

Stain results:

Iron Oxide:   Blue

References:

  • Fenton R.H., Johnson, F.B., and Zimmerman, L.E., J. Histo-chem. Cytochem., 12:153-155, 1964.
  • Luna, L.G., (ed.), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw-Hill, NY, p.181, c 1968.
RT 26603-01 Potassium Ferrocyanide Sol, 5%   500 ml 23.00 Add to Cart
RT 26603-02 Hydrochlori Acid Solution, 5% Aqueous msds 500 ml 19.00 Add to Cart

Alcoholic Stain SolutionsarrowLillie's Method for Iron

For the staining of ferric and ferrous iron.

Stain results:

Ferric Iron:   Dark Prussian Blue
Ferrous Iron:   Dark Turnbull's Blue
Background:   Light Red

References:

  • Lillie, R.D., Histopathologic Technic & Practical Histo-Chemistry, 3rd Ed., McGraw-Hill, N.Y., p. 407, c.1965.
  • Luna, G., (ed) Manual of Histologic Staining Methods of the AFIP, 3rd Ed., McGraw-Hill, N.Y., p. 182, c. 1968.
RT 26604-01 Potassium Ferrocyanide Trihydrate (for Ferric Iron) msds 50 g 32.00 Add to Cart
RT 26604-1A Potassium Ferricyanide (for Ferrous Iron) msds 50 g 11.00 Add to Cart
RT 26604-02 Hydrochloric Acid, 0.5% msds 250 ml 11.00 Add to Cart
RT 26604-03 Basic Fuchsin, 0.5% Solution msds 250 ml 16.00 Add to Cart
RT 26604-04 Acetic Acid, 1%, Aqueous msds 500 ml 21.00 Add to Cart

Alcoholic Stain SolutionsarrowOil Red O Method for Lipofuscin

Pigment stain.

Stain results:

Lipofuscin:   Red
Nuclei:   Blue

References:

  • Luna, L.G., (ED.), manual of Histologic Staining Methods of the AFIP, 3rd Edition. The AFIP, 3rd Edition, McGraw-Hill, NY, p 186, c 1968.
RT 26609-01 Oil Red O Solution, 0.5% msds 500 ml 47.00 Add to Cart
RT 26609-02 Propylene Glycol, 85%, Aqueous msds 500 ml 23.00 Add to Cart
RT 26609-03 Mayer's Hematoxylin msds 500 ml 39.00 Add to Cart

Alcoholic Stain SolutionsarrowHall's Method for Bilirubin

Stain results:

Biliverdin:   Green
Collagen:   Red
Muscle:   Yellow

References:

  • Hall, M.J., Amer. J. Clin., 34:313 –316, 1960
  • Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd Ed. McGraw-Hill, N.Y., p. 174, c. 1968
RT 26611-01 Fouchet's Reagent msds 250 ml 29.00 Add to Cart
RT 26611-02 Van Gieson Solution msds 500 ml 31.00 Add to Cart

Alcoholic Stain SolutionsarrowPrussian Blue Method for Hemosiderin

For the staining of deposits of hemosiderin.

Stain results:

Hemosiderin:   Blue or Green
Nuclei:   Red
Background:   Pink

References:

  • Perls, M., Virchow's Arch. F. Path. Anat., 39:42-48, 1867
  • Highman, B., Arch. Path., 33:937-938, 1942
  • Lillie, R.D. Histopathologic Technic and Practical Histo-chemistry, 3rd Ed. McGraw-Hill, N.Y., 1965
  • Clark, G., (ed), Staining Procedures 3rd Ed., Williams & Wilkins, Baltimore, p. 157, c. 1973
RT 26613-01 Potassium Ferrocyanide, 2%, Aqueous msds 500 ml 21.00 Add to Cart
RT 26613-02 Hydrochloric Acid, 2% msds 500 ml 23.00 Add to Cart
RT 26613-03 Safranin O, 0.2% msds 500 ml 24.00 Add to Cart
RT 26613-04 Acetic Acid, 1% Aqueous msds 500 ml 17.00 Add to Cart

Alcoholic Stain SolutionsarrowGomori's Methenamine Silver for Urate Crystals Urate crystals.

Stain results:

Urate Crystals:   Black
Background:   Green

References:

  • Sheehan, Dezna C. and Hrapchak, Barbara B.: Theory and Practice of Histotechnology, 2nd Ed.
  • The C.V. Mosby Company, St. Louis, p.225-226, c. 1980.
RT 26614-01 Silver Nitrate, 5%, Aqueous msds 250 ml 85.00 Add to Cart
RT 26614-02 Methenamine, 3%, Aqueous msds 500 ml 24.00 Add to Cart
RT 26614-03 Sodium Borate, 5%, Aqueous msds 500 ml 30.00 Add to Cart
RT 26614-04 Gold Chloride, 0.1%   250 ml 38.00 Add to Cart
RT 26614-05 Sodium Thiosulfate, 3%, Aqueous   500 ml 21.00 Add to Cart
RT 26614-06 Light Green Working Solution msds 500 ml 27.00 Add to Cart

Neurological Tissue Prepared Stains arrow13arrow13