Prepared Staining Kits for Histology and Pathology
J. NEUROLOGICAL TISSUE STAINS
Method for Supporting Elements


Ptah Method for Central Nervous Tissue
For staining microglia, fibroglia, fibrin and coarse fibrils.
Stain results:
Nuclei, Fibrin, Fibroglia, Microglia: |
|
Blue |
Elastic Fibrils, Coarse: |
|
Purplish |
References:
- AFIP Manual of Histologic Staining Techniques; (ed) G. Luna, 3rd Ed. McGraw-Hill Publications, c. 1968, p. 199
RT |
26658-01 |
Potassium Permanganate, 1%, Aqueous |
 |
500 ml |
23.00 |
Add to Cart |
RT |
26658-02 |
Phosphotungstic Acid Hematoxylin (Ptah) |
 |
500 ml |
43.00 |
Add to Cart |
RT |
26658-03 |
Oxalic Acid, 5%, Aqueous |
 |
500 ml |
30.00 |
Add to Cart |
RT |
26658-04 |
Lugol's Iodine Solution |
 |
500 ml |
23.00 |
Add to Cart |
RT |
26658-05 |
Russel's Modified Zenker's Fluid |
 |
500 ml |
32.00 |
Add to Cart |
Methods for Nerve Cells, NISSL, Granules, Negri Bodies, and Chromatolysis

Vogt's Method for Nerve Cell Products
Stain results:
Nissl Substance: |
|
Intense Purple |
Nuclei: |
|
Purple |
Background: |
|
Clear |
References:
- AFIP, Manual of Histological Staining Methods, 3rd Ed., Ed. L. Luna: New York: McGraw-Hill Publications, c. 1968, p. 212
RT |
26671-01 |
Cresyl Violet Acetate, 2%, Aqueous |
 |
100 ml |
30.00 |
Add to Cart |
RT |
26671-02 |
Acetate Buffer |
 |
500 ml |
20.00 |
Add to Cart |
RT |
26671-1A |
Cresyl Violet Acetate Working Solution |
 |
250 ml |
19.00 |
Add to Cart |
Methods for Myelin Sheaths

Kluver-Barrera Method for Myelin
and Nerve Calls
Relation of nerve cells to neutroglia, etc.
Stain results:
Myelin, including phospholipids: |
|
Blue to Green cells |
Cells and Cell products: |
|
Pink to Violet |
References:
- Kluver, H., and Barrera, E.: A method for the combined staining of cells and fibers in the nervous system. J. Nueropath. Exp. Neurol. 12:400 – 403, 1953
- AFIP, Lab Methods in Histotechnology, ed. Edna B. Prophet, Bob Mills. Jacquelyn B. Arrmyl, Leslie H. Sobin, M.D. Wash, D.C. American Registry of Pathology, c. 1992, p. 94-95.
RT |
26681-01 |
Luxol Fast Blue MBS Solution, 0.1% |
 |
250 ml |
19.00 |
Add to Cart |
RT |
26681-02 |
Cresyl Violet Acetate Solution, 0.1%, Aqueous |
 |
250 ml |
18.00 |
Add to Cart |
RT |
26681-03 |
Acetic Acid, 10%, Aqueous |
 |
250 ml |
11.00 |
Add to Cart |
RT |
26681-04 |
Lithium Carbonate Solution, 0.05%, Aqueous |
 |
250 ml |
13.00 |
Add to Cart |

Woelcker's Method for Myelin Sheath
Stain for myelin sheath, glial cells and nucleoli of neurons
Stain results:
Glial Cells, Nucleoli of neurons: |
|
Black |
Myelin sheath: |
|
Blue |
Background: |
|
Clear |
References:
- AFIP: Manual of Histological Staining Methods, 3rd ed., Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 206.
RT |
26684-01 |
Ferric Ammonium Sulfate, 2.5% Aqueous |
 |
500 ml |
26.00 |
Add to Cart |
RT |
26684-02 |
Alcoholic Hematoxylin Stock Solution, 10% |
 |
500 ml |
90.00 |
Add to Cart |
RT |
26684-03 |
Lithium Carbonate Solution, Saturated Aqueous |
 |
500 ml |
28.00 |
Add to Cart |
Methods for Nerve Fibers and Nerve Endings

Bodian's Protargol Method
For neurological tissue stains: Nerve fiber and nerve endings.
Stain results:
Nerve Fibers, Nuclei: |
|
Black |
Myelinin, Muscle, Erythrocytes, Lissamine
Fast Red Counterstain: |
|
Red |
Background-Lissamine Fast Red Counterstain: |
|
Yellow |
Background-Aniline Blue Counterstain: |
|
Blue |
References:
- Luna, L.G.,(ed), Manual of Histologic Staining Methods of the AFIP 3rd Edition, McGraw-Hill, NY, c 1968, p.195.
- Clark, G., (ed.). Staining Procedures, 3rd Edition, Williams & Wilkens Co., Baltimore, c. 1973, 98.
RT |
26693-01 |
Protargol Solution, 1% Aqueous |
 |
500 ml |
43.00 |
Add to Cart |
RT |
26693-02 |
Copper Shot |
 |
50g |
33.00 |
Add to Cart |
RT |
26693-03 |
Gold Chloride, 1% Aqueous |
 |
250 ml |
206.00 |
Add to Cart |
RT |
26693-04 |
Aniline Blue Solution, or |
|
500 ml |
42.00 |
Add to Cart |
RT |
26693-05 |
Lissamine Fast Red Solution |
 |
500 ml |
41.00 |
Add to Cart |
RT |
26693-06 |
Oxalic Acid, 2% Aqueous |
 |
250 ml |
13.00 |
Add to Cart |
RT |
26693-07 |
Sodium Thiosulfate, 5% Aqueous |
 |
500 ml |
21.00 |
Add to Cart |
RT |
26693-08 |
Phosphomolybdic Acid, 1% Aqueous |
 |
500 ml |
32.00 |
Add to Cart |
RT |
26693-09 |
Tartrazine Solution, 1.5% |
 |
500 ml |
25.00 |
Add to Cart |
RT |
26693-10 |
Hydraquinone Reducing Solution |
 |
250 ml |
14.00 |
Add to Cart |

Hirano-Zimmerman Method
for Nerve Cells and Fibers (1962)
For the staining of nerofibrils, dendrites, axis cylinders,
senile plaques, etc.
Stain results:
Nucleolus and nuclear membrane: |
|
Black |
Neurofibrils, dendrites and axis cylinders: |
|
Black |
Cytoplasm of astrocytes and the cytoplasmic
membranes of macrophages: |
|
Gray |
Senile plaques: |
|
Black |
References:
- AFIP Manual of Histological Staining Methods, 3rd ed., Ed. L.Luna: New York: McGraw-Hill Pub., c 1968, p. 198
- Hirano, A. and Zimmerman, H.M., Arch. Neurol., 6:114-122, 1962
RT |
26698-01 |
Silver Nitrate, 10% Aqueous |
 |
250 ml |
140.00 |
Add to Cart |
RT |
26698-02 |
Ammonia Water, 0.1% |
|
500 ml |
16.00 |
Add to Cart |
RT |
26698-03 |
Formalin Solution, 50% |
 |
500 ml |
23.00 |
Add to Cart |
RT |
26698-04 |
Gold Chloride, 0.05% Aqueous |
|
500 ml |
30.00 |
Add to Cart |
RT |
26698-05 |
Sodium Thiosulfate, 5% Aqueous |
 |
500 ml |
22.00 |
Add to Cart |