Prepared Staining Kits for Histology and Pathology

J. NEUROLOGICAL TISSUE STAINS

Method for Supporting Elements

Ptah Method for Central Nervous Tissue

For staining microglia, fibroglia, fibrin and coarse fibrils.

Stain results:

Nuclei, Fibrin, Fibroglia, Microglia:		Blue
Elastic Fibrils, Coarse:		Purplish

References:

• AFIP Manual of Histologic Staining Techniques; (ed) G. Luna, 3rd Ed. McGraw-Hill Publications, c. 1968, p. 199

RT	26658-01	Potassium Permanganate, 1%, Aqueous		500 ml	22.00	Add to Cart
RT	26658-02	Phosphotungstic Acid Hematoxylin (Ptah)		500 ml	42.00	Add to Cart
RT	26658-03	Oxalic Acid, 5%, Aqueous		500 ml	30.00	Add to Cart
RT	26658-04	Lugol’s Iodine Solution		500 ml	23.00	Add to Cart
RT	26658-05	Russel’s Modified Zenker’s Fluid		500 ml	32.00	Add to Cart

Methods for Nerve Cells, NISSL, Granules, Negri Bodies, and Chromatolysis

Vogt’s Method for Nerve Cell Products

Stain results:

Nissl Substance:		Intense Purple
Nuclei:		Purple
Background:		Clear

References:

• AFIP, Manual of Histological Staining Methods, 3rd Ed., Ed. L. Luna: New York: McGraw-Hill Publications, c. 1968, p. 212

RT	26671-01	Cresyl Violet Acetate, 2%, Aqueous		100 ml	30.00	Add to Cart
RT	26671-02	Acetate Buffer		500 ml	20.00	Add to Cart
RT	26671-1A	Cresyl Violet Acetate Working Solution		250 ml	19.00	Add to Cart

Methods for Myelin Sheaths

Kluver-Barrera Method for Myelin and Nerve Calls

Relation of nerve cells to neutroglia, etc.

Stain results:

Myelin, including phospholipids:		Blue to Green cells
Cells and Cell products:		Pink to Violet

References:

• Kluver, H., and Barrera, E.: A method for the combined staining of cells and fibers in the nervous system. J. Nueropath. Exp. Neurol. 12:400 – 403, 1953
• AFIP, Lab Methods in Histotechnology, ed. Edna B. Prophet, Bob Mills. Jacquelyn B. Arrmyl, Leslie H. Sobin, M.D. Wash, D.C. American Registry of Pathology, c. 1992, p. 94-95.

RT	26681-01	Luxol Fast Blue MBS Solution, 0.1%		250 ml	19.00	Add to Cart
RT	26681-02	Cresyl Violet Acetate Solution, 0.1%, Aqueous		250 ml	18.00	Add to Cart
RT	26681-03	Acetic Acid, 10%, Aqueous		250 ml	11.00	Add to Cart
RT	26681-04	Lithium Carbonate Solution, 0.05%, Aqueous		250 ml	13.00	Add to Cart

Woelcker’s Method for Myelin Sheath

Stain for myelin sheath, glial cells and nucleoli of neurons

Stain results:

Glial Cells, Nucleoli of neurons:		Black
Myelin sheath:		Blue
Background:		Clear

References:

• AFIP: Manual of Histological Staining Methods, 3rd ed., Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 206.

RT	26684-01	Ferric Ammonium Sulfate, 2.5% Aqueous		500 ml	22.00	Add to Cart
RT	26684-02	Alcoholic Hematoxylin Stock Solution, 10%		500 ml	90.00	Add to Cart
RT	26684-03	Lithium Carbonate Solution, Saturated Aqueous		500 ml	28.00	Add to Cart

Methods for Nerve Fibers and Nerve Endings

Bodian’s Protargol Method

For neurological tissue stains: Nerve fiber and nerve endings.

Stain results:

Nerve Fibers, Nuclei:		Black
Myelinin, Muscle, Erythrocytes, Lissamine Fast Red Counterstain:		Red
Background-Lissamine Fast Red Counterstain:		Yellow
Background-Aniline Blue Counterstain:		Blue

References:

• Luna, L.G.,(ed), Manual of Histologic Staining Methods of the AFIP 3rd Edition, McGraw-Hill, NY, c 1968, p.195.
• Clark, G., (ed.). Staining Procedures, 3rd Edition, Williams & Wilkens Co., Baltimore, c. 1973, 98.

RT	26693-01	Protargol Solution, 1% Aqueous		500 ml	42.00	Add to Cart
RT	26693-02	Copper Shot		50g	32.00	Add to Cart
RT	26693-03	Gold Chloride, 1% Aqueous		250 ml	206.00	Add to Cart
RT	26693-04	Aniline Blue Solution, or		500 ml	41.00	Add to Cart
RT	26693-05	Lissamine Fast Red Solution		500 ml	39.00	Add to Cart
RT	26693-06	Oxalic Acid, 2% Aqueous		250 ml	13.00	Add to Cart
RT	26693-07	Sodium Thiosulfate, 5% Aqueous		500 ml	20.00	Add to Cart
RT	26693-08	Phosphomolybdic Acid, 1% Aqueous		500 ml	31.00	Add to Cart
RT	26693-09	Tartrazine Solution, 1.5%		500 ml	24.00	Add to Cart
RT	26693-10	Hydraquinone Reducing Solution		250 ml	13.00	Add to Cart

Hirano-Zimmerman Method for Nerve Cells and Fibers (1962)

For the staining of nerofibrils, dendrites, axis cylinders, senile plaques, etc.

Stain results:

Nucleolus and nuclear membrane:		Black
Neurofibrils, dendrites and axis cylinders:		Black
Cytoplasm of astrocytes and the cytoplasmic membranes of macrophages:		Gray
Senile plaques:		Black

References:

• AFIP Manual of Histological Staining Methods, 3rd ed., Ed. L.Luna: New York: McGraw-Hill Pub., c 1968, p. 198
• Hirano, A. and Zimmerman, H.M., Arch. Neurol., 6:114-122, 1962

RT	26698-01	Silver Nitrate, 10% Aqueous		250 ml	135.00	Add to Cart
RT	26698-02	Ammonia Water, 0.1%		500 ml	16.00	Add to Cart
RT	26698-03	Formalin Solution, 50%		500 ml	23.00	Add to Cart
RT	26698-04	Gold Chloride, 0.05% Aqueous		500 ml	30.00	Add to Cart
RT	26698-05	Sodium Thiosulfate, 5% Aqueous		500 ml	22.00	Add to Cart

Nuclear Stains