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Electron Microscopy Sciences

Histology and Light Microscopy

arrow13Prepared Staining Kits for Histology and Pathology

J. NEUROLOGICAL TISSUE STAINS

Method for Supporting Elements

msdsAlcoholic Stain SolutionsarrowPtah Method for Central Nervous Tissue

For staining microglia, fibroglia, fibrin and coarse fibrils.

Stain results:

Nuclei, Fibrin, Fibroglia, Microglia:   Blue
Elastic Fibrils, Coarse:   Purplish

References:

  • AFIP Manual of Histologic Staining Techniques; (ed) G. Luna, 3rd Ed. McGraw-Hill Publications, c. 1968, p. 199
RT 26658-01 Potassium Permanganate, 1%, Aqueous msds 500 ml 20.00 Add to Cart
RT 26658-02 Phosphotungstic Acid Hematoxylin (Ptah) msds 500 ml 39.00 Add to Cart
RT 26658-03 Oxalic Acid, 5%, Aqueous msds 500 ml 30.00 Add to Cart
RT 26658-04 Lugol’s Iodine Solution msds 500 ml 27.00 Add to Cart
RT 26658-05 Russel’s Modified Zenker’s Fluid msds 500 ml 32.00 Add to Cart

Methods for Nerve Cells, NISSL, Granules, Negri Bodies, and Chromatolysis

Alcoholic Stain SolutionsarrowVogt’s Method for Nerve Cell Products

Stain results:

Nissl Substance:   Intense Purple
Nuclei:   Purple
Background:   Clear

References:

  • AFIP, Manual of Histological Staining Methods, 3rd Ed., Ed. L. Luna: New York: McGraw-Hill Publications, c. 1968, p. 212
RT 26671-01 Cresyl Violet Acetate, 2%, Aqueous msds 100 ml 30.00 Add to Cart
RT 26671-02 Acetate Buffer msds 500 ml 20.00 Add to Cart
RT 26671-1A Cresyl Violet Acetate Working Solution msds 250 ml 19.00 Add to Cart

Methods for Myelin Sheaths

Alcoholic Stain SolutionsarrowKluver-Barrera Method for Myelin and Nerve Calls

Relation of nerve cells to neutroglia, etc.

Stain results:

Myelin, including phospholipids:   Blue to Green cells
Cells and Cell products:   Pink to Violet

References:

  • Kluver, H., and Barrera, E.: A method for the combined staining of cells and fibers in the nervous system. J. Nueropath. Exp. Neurol. 12:400 – 403, 1953
  • AFIP, Lab Methods in Histotechnology, ed. Edna B. Prophet, Bob Mills. Jacquelyn B. Arrmyl, Leslie H. Sobin, M.D. Wash, D.C. American Registry of Pathology, c. 1992, p. 94-95.
RT 26681-01 Luxol Fast Blue MBS Solution, 0.1% msds 250 ml 17.00 Add to Cart
RT 26681-02 Cresyl Violet Acetate Solution, 0.1%, Aqueous msds 250 ml 18.00 Add to Cart
RT 26681-03 Acetic Acid, 10%, Aqueous msds 250 ml 11.00 Add to Cart
RT 26681-04 Lithium Carbonate Solution, 0.05%, Aqueous msds 250 ml 13.00 Add to Cart

Alcoholic Stain SolutionsarrowWoelcker’s Method for Myelin Sheath

Stain for myelin sheath, glial cells and nucleoli of neurons

Stain results:

Glial Cells, Nucleoli of neurons:   Black
Myelin sheath:   Blue
Background:   Clear

References:

  • AFIP: Manual of Histological Staining Methods, 3rd ed., Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 206.
RT 26684-01 Ferric Ammonium Sulfate, 2.5% Aqueous msds 500 ml 20.00 Add to Cart
RT 26684-02 Alcoholic Hematoxylin Stock Solution, 10% msds 500 ml 90.00 Add to Cart
RT 26684-03 Lithium Carbonate Solution, Saturated Aqueous msds 500 ml 28.00 Add to Cart

Methods for Nerve Fibers and Nerve Endings

Alcoholic Stain SolutionsarrowBodian’s Protargol Method

For neurological tissue stains: Nerve fiber and nerve endings.

Stain results:

Nerve Fibers, Nuclei:   Black
Myelinin, Muscle, Erythrocytes, Lissamine
Fast Red Counterstain:
  Red
Background-Lissamine Fast Red Counterstain:   Yellow
Background-Aniline Blue Counterstain:   Blue

References:

  • Luna, L.G.,(ed), Manual of Histologic Staining Methods of the AFIP 3rd Edition, McGraw-Hill, NY, c 1968, p.195.
  • Clark, G., (ed.). Staining Procedures, 3rd Edition, Williams & Wilkens Co., Baltimore, c. 1973, 98.
RT 26693-01 Protargol Solution, 1% Aqueous msds 500 ml 38.00 Add to Cart
RT 26693-02 Copper Shot msds 50g 30.00 Add to Cart
RT 26693-03 Gold Chloride, 1% Aqueous msds 250 ml 206.00 Add to Cart
RT 26693-04 Aniline Blue Solution, or   500 ml 41.00 Add to Cart
RT 26693-05 Lissamine Fast Red Solution msds 500 ml 37.00 Add to Cart
RT 26693-06 Oxalic Acid, 2% Aqueous msds 250 ml 13.00 Add to Cart
RT 26693-07 Sodium Thiosulfate, 5% Aqueous msds 500 ml 20.00 Add to Cart
RT 26693-08 Phosphomolybdic Acid, 1% Aqueous msds 500 ml 31.00 Add to Cart
RT 26693-09 Tartrazine Solution, 1.5% msds 500 ml 24.00 Add to Cart
RT 26693-10 Hydraquinone Reducing Solution msds 250 ml 13.00 Add to Cart

Alcoholic Stain SolutionsarrowHirano-Zimmerman Method for Nerve Cells and Fibers (1962)

For the staining of nerofibrils, dendrites, axis cylinders, senile plaques, etc.

Stain results:

Nucleolus and nuclear membrane:   Black
Neurofibrils, dendrites and axis cylinders:   Black
Cytoplasm of astrocytes and the cytoplasmic
membranes of macrophages:
  Gray
Senile plaques:   Black

References:

  • AFIP Manual of Histological Staining Methods, 3rd ed., Ed. L.Luna: New York: McGraw-Hill Pub., c 1968, p. 198
  • Hirano, A. and Zimmerman, H.M., Arch. Neurol., 6:114-122, 1962
RT 26698-01 Silver Nitrate, 10% Aqueous msds 250 ml 135.00 Add to Cart
RT 26698-02 Ammonia Water, 0.1%   500 ml 16.00 Add to Cart
RT 26698-03 Formalin Solution, 50% msds 500 ml 23.00 Add to Cart
RT 26698-04 Gold Chloride, 0.05% Aqueous   500 ml 30.00 Add to Cart
RT 26698-05 Sodium Thiosulfate, 5% Aqueous msds 500 ml 22.00 Add to Cart

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