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Electron Microscopy Sciences

ImmunoGold Reagents

pdf downloadarrow13Immuno Gold Custom Labeling Service

CrySEM Workshop DetailsThe standard series of AURION ImmunoGold reagents is intended for the two and three step detection of antigens. Although these approaches cover the majority of applications occasionally there may exist a need for a direct label where the gold particles are coupled directly to the primary antibody or, more general, to a protein or peptide with specific binding properties.

To fulfill these needs AURION and EMS offers a custom labeling service for antibodies, antibody fragments, proteins, and peptides with gold particles of any requested size, including ULTRA SMALL gold clusters. AURION designs and manufactures such custom conjugates in close co-operation and in agreement with the principal investigator.

Starting material

As a rule AURION will only accept pure substances as starting material, either lyophilized or dissolved in a medium of known chemical composition. Interfering substances must not be present.

Quality Control Criteria

The custom prepared product is quality controlled by AURION according to our in-house standards with respect to size distribution and clustering. In agreement with the principal investigator the binding substance's specific quality control criteria are determined. These are tailored for each individual assignment. As a matter of course the custom conjugate is approved only when both sets of quality control criteria have been met.

A few examples

AURION successfully conjugated a number of polyclonal antibodies, mouse monoclonal antibodies, F(ab')2 and F(ab) fragments, high and low density lipoproteins, as well as peptides such as an Angiotensin derivative (octapeptide), Interleukin II, Tumour Necrosis Factor and a number of peptide hormones.

The efficiency of coupling (recovery of protein) for the above mentioned custom conjugates has been greater than 70% on all occasions, while efficiencies up to 90% are no exception.

No cure, no pay

The custom labeling service is on the basis of "no cure, no pay," with the exception that an intake fee applies to each actual laboratory attempt.

We invite you to contact us directly if you require information which is more tailored to your specific applications.

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