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Electron Microscopy Sciences

Illuminators, Magnifiers, Microscopes & Graticules

Downloadable PDFarrow13Eyepiece Graticules

The standard sizes of eyepiece graticules used to be 16, 19, and 21 mm. For your convenience, this has been greatly expanded to accommodate a variety of equipment without the need to special order. The following table will assist you in determining appropriate eyepieces for common brands.

Brand Reticle Diameter Marking on Eyepiece
Olympus Eyepieces 24 mm WHN10X, WHN10X-H WH10X, WH10X-H, WH15X
WHS10X-H, WHS15X-H CWH10X, CWH10X-H, CWH30X
GW10X GWH10X
GSWH10X, GSWH15X, GSWH20X, GSWH30X WH12.5X, WH12.5X-H
WHSZ10X-H, WHSZ15X-H, WHSZ20X-H, WHSZ30X-H GSWH10X-H, GSWH15X-H, GSWH20X-H, GSWH30X-H
20.4 mm WHK8X, WHK10X, WHK15X PE 2.5X/ 3.3X/ 4X/ 5X
NWHK10X, (*WHK10X-H cannot be installed) GWH15X-D, GWH15X-CD
NFK 2.5X/ 3.3X/ 5X/ 6.7X  
Nikon Eyepieces 27 mm CFI 10x, CFI 12.5x, CFI 15x, CFI UW 10x CFUW10X, CFUWN 10X
E1-CFI 10x, E2-CFI 10x, E2-CFI 15x SME 10x /23, SME 10x /21
SMZ 10 UW15x /17, SMZ 10 UW20x/15  
25 mm C-W 10x B/22 (C-W 10X A/22) L-W10x ESD (FOV 22)
SMZ U UW10x A/24, SMZ U UW15x  
Leica Eyepieces 26 mm 11507807 (S10x/22B.M) 11507808 (S10x/25Br.M)
11507801 (10x/20Br.M) 11507802 (10x/20Br.M)
11506515 (12.5x/16Br.M)  
23 mm 10447160 (10x/21B) 10445301 (16x/14B)
10445302 (25x/9.5B) 10445303 (40x/6B)
24.5 mm 10450023 (10x/23B) 10450024 (16x /15B)
10450025 (25x/9.5B) 10450026 (40x/6B)
10 446 333 (10x/23, adjustable) 13 613 532 (10x/20, focusing)
10 446 355 (16x/16, adjustable) 10 446 357 (20x/12, adjustable)
10 446 329 (10x/23B, adjust.f/eyeglasses) 10 447 131 (10x/23, adjustable)
10 447 133 (16x/16, adjustable) 10 447 135 (20x/126, adjustable)
10 447 137 (for eyeglasses10x/23B, adjustable) 10 447 139 (for eyeglasses16x/15B, adjustable)
10 445 302 (25x/9.5B, adjust.f/eyeglasses) 10 445 303 (40x/6B, adjust.f/eyeglasses)
10 447 280 (10x/20B, adjust.f/eyeglasses)  
Zeiss Eyepieces 26 mm E-PL 10x/20 Br. Foc PL 10x/21 Br Foc
PL 10x/23 Br. Foc  
23 mm WF 10x/18 WF 10X/20
21 mm PL16x/16 Br. Foc W 25x/10 foc
Kpl-W 10x/18 [#46 40 43-9902]  
This list is not comprehensive so please inquire if your eyepiece or brand is not listed.

arrow12A. Size and Position Scales

Single Lines - NE50arrow10Single Lines - NE50

For measurement of large objects in conjunction with a graduated mechanical stage, and for alignment as well. Image covers entire field of view.

Pattern Description Dia Cat # Qty Price  
NE50 Single Line, nominal width 0.02 mm.
Surface chrome image.
16 mm 67982-16 each 99.00 Add to Cart
19 mm 67982-19 each 99.00 Add to Cart
21 mm 67982-21 each 99.00 Add to Cart
Cross Lines - NE8arrow10Cross Lines - NE8, NE81, NE82

Used as NE50 but for measurements in two directions and for sighting and alignment. Image covers the entire field of view.

Pattern Description Dia Cat # Qty Price  
NE8 Cross lines with surface chrome image.
Nominal line width 0.02 mm.
16 mm 67983-01 each 99.00 Add to Cart
19 mm 67983-05 each 99.00 Add to Cart
20.4 mm 67983-20.4 each 99.00 Add to Cart
21 mm 67983-10 each 99.00 Add to Cart
23 mm 67983-23 each 99.00 Add to Cart
24.5 mm 67983-24.5 each 99.00 Add to Cart
25 mm 67983-025 each 99.00 Add to Cart
26 mm 67983-26 each 99.00 Add to Cart
27 mm 67983-27 each 99.00 Add to Cart
NE81 Cross lines with surface chrome image.
Nominal line width 0.04 mm.
16 mm 67983-20 each 99.00 Add to Cart
19 mm 67983-25 each 99.00 Add to Cart
21 mm 67983-30 each 99.00 Add to Cart
NE82 Cross lines with surface chrome image.
Nominal line width 0.005 mm.
16 mm 67984-16 each 99.00 Add to Cart
19 mm 67984-19 each 99.00 Add to Cart
21 mm 67984-21 each 99.00 Add to Cart
Broken Cross Lines - NE56arrow10Broken Cross Lines - NE56

Used as above. Broken lines enable fine detail to be seen at the breaks. A thin boundary would be lost behind a a continuous line. Image covers the entire field of view.

Pattern Description Dia Cat # Qty Price  
NE56 Broken cross lines.
Surface chrome image.
16 mm 67985-16 each 99.00 Add to Cart
19 mm 67985-19 each 99.00 Add to Cart
21 mm 67985-21 each 99.00 Add to Cart
Crossed Gauge Lines - NE53arrow10Crossed Gauge Lines - NE53, N54

Use as crossed lines, but for the measuring of distances between the lines. Greater accuracy can be obtained by locating the specimen detail between the graticules gauge pair.

Pattern Description Dia Cat # Qty Price  
NE53 Two vertical lines 0.10 mm apart with horizontal line. Image covers the entire field of view.
Surface chrome image.
16 mm 67986-16 each 119.00 Add to Cart
19 mm 67986-19 each 119.00 Add to Cart
21 mm 67986-21 each 119.00 Add to Cart
NE54 Two vertical lines 0.2 mm apart. 16 mm 67975-16 each 105.00 Add to Cart
19 mm 67975-19 each 105.00 Add to Cart
21 mm 67975-21 each 105.00 Add to Cart
Custom 67975-50 each 205.00 Add to Cart
arrow10Horizontal & Vertical Scales - NE1, NE2, NE5, NE20, NE28, NE31, NE41, NE120
Vertical Scale - NE2Horizontal Scale - NE1

These scales are used for the measuring of lengths of specimen or distances between points on a variety of different shaped objects. NE1 scale shown: This eyepiece graticule has an overall length of 10.00 mm with 100 subdivisions of 0.1 mm. When used with a x10 objective, each division will represent 10 microns on the specimen. By dividing the division of the chosen graticule by the magnification of the objective, one obtains an approximate value that each division will represent on the stage.

Pattern Description Dia Cat # Qty Price  
NE1 Horizontal micrometer scale 10 mm long with 0.1 mm subdivisions.
Surface chrome image.
16 mm 68010-16 each 94.00 Add to Cart
19 mm 68010-19 each 94.00 Add to Cart
20.4 mm 68010-20.4 each 94.00 Add to Cart
21 mm 68010-21 each 94.00 Add to Cart
23 mm 68010-23 each 94.00 Add to Cart
24.5 mm 68010-24.5 each 112.00 Add to Cart
25 mm 68010-25 each 112.00 Add to Cart
26 mm 68010-26 each 112.00 Add to Cart
27 mm 68010-27 each 112.00 Add to Cart
NE2 Vertical micrometer scale 10 mm long with 0.1 mm subdivisions.
Surface chrome image.
16 mm 68006-16 each 109.00 Add to Cart
19 mm 68006-19 each 109.00 Add to Cart
21 mm 68006-21 each 109.00 Add to Cart
NE5 Horizontal micrometer scale 5 mm long with 0.05 mm subdivisions.
Surface chrome image.
16 mm 67988-16 each 107.00 Add to Cart
19 mm 67988-19 each 107.00 Add to Cart
21 mm 67988-21 each 107.00 Add to Cart
NE20 Horizontal scale 0.10 inch long with 100 subdivisions.
Surface scale image.
16 mm 67987-16 each 109.00 Add to Cart
19 mm 67987-19 each 109.00 Add to Cart
21 mm 67987-21 each 109.00 Add to Cart
NE28 Horizontal micrometer scale.
1 mm long with 100 subdivisions.
Surface chrome image.
16 mm 67992-16 each 119.00 Add to Cart
19 mm 67992-19 each 119.00 Add to Cart
21 mm 67992-21 each 119.00 Add to Cart
NE31 Horizontal scale 0.5" long with 100 divisions of 0.0005" 16 mm 67977-16 each 101.00 Add to Cart
19 mm 67977-19 each 101.00 Add to Cart
21 mm 67977-21 each 101.00 Add to Cart
Custom 67977-50 each 204.00 Add to Cart
NE41 Horizontal micrometer scale 10 mm long with 200 subdivisions.
Surface chrome image.
16 mm 67993-16 each 110.00 Add to Cart
19 mm 67993-19 each 110.00 Add to Cart
21 mm 67993-21 each 110.00 Add to Cart
NE120 Horizontal Micrometer 20 mm long with 100 divisions of 0.10 mm. 21 mm 67976-21 each 101.00 Add to Cart
Custom 67976-50 each 205.00 Add to Cart
arrow10Crossed Scales - NE17, NE18, NE70, NE72
Crossed -NE72Crossed - NE17

Used as a horizontal and a vertical scale, they are especially useful when interested in measurement in different axis.

Pattern Description Dia Cat # Qty Price  
NE17 Crossed micrometer scales 10 mm long with 0.1 mm subdivisions.
Surface chrome image.
16 mm 67989-16 each 119.00 Add to Cart
19 mm 67989-19 each 119.00 Add to Cart
20.4 mm 67989-20.4 each 119.00 Add to Cart
21 mm 67989-21 each 119.00 Add to Cart
23 mm 67989-23 each 119.00 Add to Cart
24.5 mm 67989-24.5 each 119.00 Add to Cart
25 mm 67989-25 each 119.00 Add to Cart
26 mm 67989-26 each 119.00 Add to Cart
27 mm 67989-27 each 119.00 Add to Cart
NE18 Crossed micrometer scales 5 mm long with 0.05 mm subdivisions.
Surface chrome image.
16 mm 67994-16 each 119.00 Add to Cart
19 mm 67994-19 each 119.00 Add to Cart
21 mm 67994-21 each 119.00 Add to Cart
NE72 Crossed Micrometer scale 20 mm long with 200 divisions of 0.10 mm. 23 mm 67978-23 each 101.00 Add to Cart
24 mm 67978-24 each 203.00 Add to Cart
26 mm 67978-26 each 145.00 Add to Cart
27 mm 67978-27 each 145.00 Add to Cart
Custom 67978-50 each 181.00 Add to Cart
NE70 Crossed Micrometer scale (Imperial). 0.8' long with 400 divisions of 0.002". 21 mm 67978-21 each 99.00 Add to Cart
23 mm 67979-23 each 181.00 Add to Cart
24 mm 67979-24 each 145.00 Add to Cart
26 mm 67979-26 each 145.00 Add to Cart
27 mm 67979-27 each 145.00 Add to Cart
Custom 67979-50 each 181.00 Add to Cart
arrow10Scales with Cross Lines - NE7, NE77, NE777
Scales with Cross Lines - NE7NScales with Cross Lines - NE7

Used as a horizontal and a vertical scale, they are especially useful when interested in measurement in different axis.

Pattern Description Dia Cat # Qty Price  
NE7 Horizontal micrometer scale 10 mm long with cross lines at 0.10 mm spacing.
Surface chrome image.
16 mm 68013-16 each 110.00 Add to Cart
19 mm 68013-19 each 110.00 Add to Cart
20.4 mm 68013-20.4 each 110.00 Add to Cart
21 mm 68013-21 each 110.00 Add to Cart
23 mm 68013-23 each 110.00 Add to Cart
24.5 mm 68013-24.5 each 135.00 Add to Cart
25 mm 68013-25 each 135.00 Add to Cart
26 mm 68013-26 each 135.00 Add to Cart
27 mm 68013-27 each 135.00 Add to Cart
NE7N Horizontal scale 10 mm/0.10 mm + crossline + square.
Surface chrome image.
16 mm 67979-16 each 119.00 Add to Cart
19 mm 67979-19 each 119.00 Add to Cart
21 mm 67979-21 each 119.00 Add to Cart
NE77 Horizontal micrometer scale 5 mm long in 100 divisions with cross line.
Surface chrome image.
16 mm 68012-16 each 110.00 Add to Cart
19 mm 68012-19 each 110.00 Add to Cart
21 mm 68012-21 each 110.00 Add to Cart
NE777 Horizontal micrometer scale 0.5" long with divisions of 0.005" and cross lines. 16 mm 67980-16 each 105.00 Add to Cart
19 mm 67980-19 each 105.00 Add to Cart
21 mm 67980-21 each 105.00 Add to Cart
Custom 67980-50 each 213.00 Add to Cart

arrow12B. Squares and Grids

Note: These scales may need to be calibrated, according to intended use. There are a number of uses for the grids and squares listed and they will largely depend on the individual user's application.

Eyepiece Scales

  • Sectoring: A squared graticule might be used for the systematic examination of a specimen. Some of the squared patterns are numbered to aid in the identification of areas of interest. Sectioning is particularly useful for making drawings of specimens onto graph paper. The chess-board type of pattern helps the user to distinguish the position being examined: the darker squares are translucent, while the lighter ones are transparent, avoiding eyestrain in prolonged counting as may be necessary in hematology. These patterns provide the same advantages when used with image analysis and capture devices.
  • Counting: A squared graticule can be used for counting. Here, the basic principle is that a small area of the specimen is analyzed in order to obtain information about the total area. This minimizes wasteful work, enabling simple analysis of a paricular area. An example of this would be the comparison of large to small particles in a specimen. By using the Miller graticule (NE57) only the smaller particles in the small square are counted, the result being multiplied by ten for comparison with the number of larger particles in the large square.
  • Squared Grids: Squared grids can be used in particle size analysis as simple technical aids where sophisticated image analysis systems are not required. The areas of the particles to be measured can be estimated by simply counting the number of squares occupied by those particles. It is necessary to estimate fractions of a square or make a rule (e.g. count as a square all partly covered squares at the right and bottom sides of the grid, and ignore partly covered squares at the left and upper sides of the squares). This method would only be useful for a fairly crude estimation of a large diameter.
Squared Grids - NE10arrow10Squared Grids - NE10, NE11, NE34

Simple grids are convenient for making sketches of the obseved speimen on graph paper. They are also useful for particle counting.

Pattern Description Dia Cat # Qty Price  
NE10 Grid (net) 0.50 mm pitch (distance of center bar to center bar).
Surface chrome image.
16 mm 68011-16 each 110.00 Add to Cart
19 mm 68011-19 each 110.00 Add to Cart
21 mm 68011-21 each 110.00 Add to Cart
NE11 Grid (net) 1.00 mm pitch (distance of center bar to center bar).
Surface chrome image.
16 mm 68015-16 each 114.00 Add to Cart
19 mm 68015-19 each 114.00 Add to Cart
21 mm 68015-21 each 114.00 Add to Cart
NE34 Grid (net) 0.10 mm squares.
Surface chrome image.
16 mm 68018-16 each 119.00 Add to Cart
19 mm 68018-19 each 119.00 Add to Cart
21 mm 68018-21 each 119.00 Add to Cart
Indexed Grids - NE71arrow10Indexed Grids - NE10A, NE11A, NE34A, NE71

Useful for particle counting, particularly when reference is needed between workers. Also, they are useful for area of specimen determinations.

Pattern Description Dia Cat # Qty Price  
NE10A Numbered grid 5 mm x 5 mm, 0.5 mm pitch. Marked 1-10 and A-J. Surface chrome image. 16 mm 68011-16A each 110.00 Add to Cart
19 mm 68011-19A each 110.00 Add to Cart
21 mm 68011-21A each 110.00 Add to Cart
NE11A Numbered grid 10 mm x 10 mm, 1.0 mm pitch. Marked 1-10 and A-J. Surface chrome image. 16 mm 68016-16 each 92.00 Add to Cart
19 mm 68016-19 each 92.00 Add to Cart
20.4 mm 68016-20.4 each 92.00 Add to Cart
21 mm 68016-21 each 92.00 Add to Cart
23 mm 68016-23 each 92.00 Add to Cart
24.5 mm 68016-24.5 each 112.00 Add to Cart
25 mm 68016-25 each 112.00 Add to Cart
26 mm 68016-26 each 112.00 Add to Cart
27 mm 68016-27 each 112.00 Add to Cart
NE34A Numbered grid 1 mm x 1 mm, 0.1 mm pitch. Marked 1-10 and A-J. Surface chrome image. 16 mm 68018-16A each 119.00 Add to Cart
19 mm 68018-19A each 119.00 Add to Cart
21 mm 68018-21A each 119.00 Add to Cart
NE71 Index Pattern 20x20. Grid of 0.50 mm squares. 21 mm 67981-21 each 105.00 Add to Cart
23 mm 67981-23 each 105.00 Add to Cart
24 mm 67981-24 each 145.00 Add to Cart
26 mm 67981-26 each 145.00 Add to Cart
Custom 67981-50 each 186.00 Add to Cart
Indexed Grids - NE35arrow10Indexed Grids - NE35

Useful for area of specimen determinations, especially rectangular shapes, also for particle counting.

Pattern Description Dia Cat # Qty Price  
NE35 Numbered grid 10 mm x 10 mm.
1 mm indexed squares.
Surface chrome image.
16 mm 68014-16 each 114.00 Add to Cart
19 mm 68014-19 each 114.00 Add to Cart
21 mm 68014-21 each 114.00 Add to Cart
Chessboard Squares - NE15arrow10Chessboard Squares - NE15

The dark squares are translucent. Used as an alternative to simple grids for area of specimen determination and particle counting. Alternative light and dark squares help reduce eyestrain.

Pattern Description Dia Cat # Qty Price  
NE15 Chessboard (net) 2.0 mm squares.
Surface chrome image.
16 mm 68008-16 each 230.00 Add to Cart
19 mm 68008-19 each 230.00 Add to Cart
21 mm 68008-21 each 230.00 Add to Cart
Squares - NE38arrow10Squares - NE38

Combines three areas in one for convenience, giving area ratios A:B of 1:3 and B:C of 1:2.

Pattern Description Dia Cat # Qty Price  
NE38 Squares 10 mm, 7 mm & 4 mm.
Glass sandwich.
16 mm 68017-16 each 119.00 Add to Cart
19 mm 68017-19 each 119.00 Add to Cart
21 mm 68017-21 each 119.00 Add to Cart
Miller Squares - NE57arrow10Miller Squares - NE57

The ratio of large to small squares is 9:1. Originally designed for hematology, they can be utilized for rapid counting of any evenly spread field of particle.

References

  • American J. of Clin. Pathol. Vol. 20, 1950, page 1079. "Time Saving Device For Counting Reticulocyte." G.Brescher and Schneiderman.
  • Practicle Haemotology. J.D. Dacy. Published by J.A. Churchill. 2nd Edition 1956 page 25.
Pattern Description Dia Cat # Qty Price  
NE57 Miller 7 x 7 mm grid.
Glass sandwich.
16 mm 68020-16 each 119.00 Add to Cart
19 mm 68020-19 each 119.00 Add to Cart
20.4 mm 68020-20.4 each 119.00 Add to Cart
21 mm 68020-21 each 119.00 Add to Cart
23 mm 68020-23 each 119.00 Add to Cart
24.5 mm 68020-24.5 each 130.00 Add to Cart
25 mm 68020-25 each 130.00 Add to Cart
26 mm 68020-26 each 130.00 Add to Cart
27 mm 68020-27 each 130.00 Add to Cart
Whipple Grid - NE29arrow10Whipple Grid - NE29

Originally designed for water particle analysis, but may be used for other aspects of particle counting. Grid shown: Ratio of full square to smallest is 50:1. Area is 2500:1.

Reference

  • Microscopy of Drinking Water.
Pattern Description Dia Cat # Qty Price  
NE29 Whipple grid 100 squares in 7 mm area.
Surface chrome image.
16 mm 68009-16 each 122.00 Add to Cart
19 mm 68009-19 each 122.00 Add to Cart
20.4 mm 68009-20.4 each 122.00 Add to Cart
21 mm 68009-21 each 122.00 Add to Cart
23 mm 68009-23 each 122.00 Add to Cart
24.5 mm 68009-24.5 each 122.00 Add to Cart
25 mm 68009-25 each 136.00 Add to Cart
26 mm 68009-26 each 136.00 Add to Cart
27 mm 68009-27 each 136.00 Add to Cart

arrow12C. Gauges

Concentric Circles - NE42arrow10Concentric Circles - NE42, NE43, NE44, NE47

Can be used for two-way measurement when calibrated as a micrometer.

Pattern Description Dia Cat # Qty Price  
NE42 Concentric circle 0.25 mm - 2.5 mm dia. 10 circles.
Surface chrome image.
16 mm 68019-16 each 119.00 Add to Cart
19 mm 68019-19 each 119.00 Add to Cart
21 mm 68019-21 each 119.00 Add to Cart
NE43 Concentric circle 0.5 mm - 5 mm dia. 10 circles.
Surface chrome image.
16 mm 67997-16 each 119.00 Add to Cart
19 mm 67997-19 each 119.00 Add to Cart
21 mm 67997-21 each 119.00 Add to Cart
NE44 Concentric circle 1 mm - 10 mm dia. 10 circles.
Surface chrome image.
16 mm 67998-16 each 119.00 Add to Cart
19 mm 67998-19 each 119.00 Add to Cart
20.4 mm 67998-20.4 each 119.00 Add to Cart
21 mm 67998-21 each 119.00 Add to Cart
23 mm 67998-23 each 119.00 Add to Cart
24.5 mm 67998-24.5 each 119.00 Add to Cart
25 mm 67998-25 each 119.00 Add to Cart
26 mm 67998-26 each 119.00 Add to Cart
27 mm 67998-27 each 119.00 Add to Cart
NE47 Concentric circle 2 mm - 20 mm dia. 10 circles.
Surface chrome image.
16 mm 67999-16 each 119.00 Add to Cart
19 mm 67999-19 each 119.00 Add to Cart
21 mm 67999-21 each 119.00 Add to Cart
Concentric Circles and Cross Scales, NE48arrow12Concentric Circles and Cross Scales - NE48
Pattern Description Dia Cat # Qty Price  
NE48 Concentric circles. 10 circles, 1 mm-10 mm with graduated cross hairs. 16 mm 67991-16 each 109.00 Add to Cart
19 mm 67991-19 each 109.00 Add to Cart
21 mm 67991-21 each 109.00 Add to Cart
23 mm 67991-23 each 109.00 Add to Cart
Custom 67991-50 each 225.00 Add to Cart
Concentric Circle - NE22arrow10Concentric Circle - NE22

This design leaves the circle clear of obstruction. In addition the intermediate lines are broken to improve ease of reading.

Pattern Description Dia Cat # Qty Price  
NE22 Concentric circle 0.5 mm - 12 mm dia. 24 circles.
Surface chrome image.
16 mm 68000-16 each 119.00 Add to Cart
19 mm 68000-19 each 119.00 Add to Cart
21 mm 68000-21 each 119.00 Add to Cart
Gauge Pairs - NE19arrow10Gauge Pairs - NE19

Gauge pairs occupying a field of view of 10 mm. Each gauge is proportional to its adjacent number. The approximately size of the smallest pair = 0.1 mm.

Pattern Description Dia Cat # Qty Price  
NE19 Gauge pairs.
Surface chrome image.
16 mm 68001-16 each 110.00 Add to Cart
19 mm 68001-19 each 110.00 Add to Cart
21 mm 68001-21 each 110.00 Add to Cart
Full Protractor - NE45Half Protractor - NE25arrow10Protractors - NE25, NE45
Pattern Description Dia Cat # Qty Price  
NE25 Half protractor scale. 10 mm diameter divided in degrees.
Surface chrome image.
16 mm 67996-16 each 122.00 Add to Cart
19 mm 67996-19 each 122.00 Add to Cart
21 mm 67996-21 each 122.00 Add to Cart
NE45 Full protractor scale. 10 mm diameter divided in degrees.
Surface chrome image.
16 mm 68002-16 each 119.00 Add to Cart
19 mm 68002-19 each 119.00 Add to Cart
21 mm 68002-21 each 119.00 Add to Cart

arrow12D. Particle Sizing and Distributing

The use of the eyepiece graticules shown in this section makes it possible to analyze specimens containing particles as an alternative, or in addition to, sieving. Graticules for particle size analysis are particularly popular when there are only limited quantities of particles or where particles are smaller than 50 microns in diameter. Typical substances analyzed are sand grains, soil particles, plant seeds, fertilizers, abrasives, liquid droplets, pigments, pulverized coal, silica, fibers, and fine dust.

The basic principle employed is to compare particles to the globes and circles of varying sizes that appear on the graticule – dark particles being compared to solids globes, and light or transparent ones to the circles. Naturally the procedure varies with the graticule concerned, more information about which is given below.

Please note that for calibration, the circles and globes will represent particles smaller in diameter by the magnification of the objective.

Paterson Globes and Circles - NG1arrow10Paterson Globes and Circles - NG1

The graticule consists of a central rectangle, sub-divided into nine smaller rectangles, with a number of increasing circles outside the top and bottom horizontal edges. The marked figures are the diameters of the circles in units. 250 units represent the horizontal length of the large rectangle. Rectangle size is 4.5 mm x 2.025 mm. Circle sizes in microns are 450, 360, 270, 225, 180, 145, 110, 74, 37, and 18.

Pattern Description Dia Cat # Qty Price  
NG1 Paterson globes/circles.
Surface chrome image.
16 mm 68031-16 each 224.00 Add to Cart
19 mm 68031-19 each 224.00 Add to Cart
21 mm 68031-21 each 224.00 Add to Cart
Porton - NG2arrow10Porton - NG2

The circle areas of the Porton greaticules increases with Root 2 progression, as do the divisions on the right hand side of the rectangle. These divisions are numbered for convenience. The rectangle size is 4.5 mm x .25 mm. Circle sizes in microns are 560, 400, 280, 200, 140, 100, 70, 50, and 35. The specimen is racked on the mechanical stage of the microscope and traverses are taken right across the deposit, sizing all the particles encountered.

Pattern Description Dia Cat # Qty Price  
NG2 Original Porton globes/circles.
Surface chrome image.
16 mm 68024-16 each 242.00 Add to Cart
19 mm 68024-19 each 226.00 Add to Cart
21 mm 68024-21 each 226.00 Add to Cart
New Porton - NG12arrow10New Porton - NG12

The NG12 is particularly useful since the array of globes and circles are conveniently close to where the particles pass. At the end of each band of the sample the mechanical stage is traversed vertically to take in the next band until the whole sample has been covered.

Pattern Description Dia Cat # Qty Price  
NG12 Modified Porton pattern globes/circles.
Surface chrome image.
16 mm 68032-16 each 224.00 Add to Cart
19 mm 68032-19 each 224.00 Add to Cart
21 mm 68032-21 each 224.00 Add to Cart
British Standard Graticule - NG10arrow10British Standard Graticule - NG10

In this graticule the circle areas double progressively, hence the diameters alter by Root 2, so that the size classes can form a continuation of the standard series of sieves for particle sizing. Each particle is assigned to a size class defined by two adjacent circles, which represent the size limits of that class. Thus the distribution of size is obtained in terms of the diameter of circles having the same projected area as the particles. This method will cover particles in the range 150 micron to 0.38 micron. The size distributions with respect to their number and weight are determined separately. Final results are calculated as cumulative percents.

Originally designed by the National Coal Board for use in coal mining.

Pattern Description Dia Cat # Qty Price  
NG10 British Standard (BS3625/BS3260)
Globes & Circles.
Surface chrome image.
16 mm 68026-16 each 242.00 Add to Cart
19 mm 68026-19 each 242.00 Add to Cart
21 mm 68026-21 each 242.00 Add to Cart
Fairs - NG5arrow10Fairs - NG5

Designed to extend the sizing range of globe and circle graticules.

Example: Used in conjunction with NG2 the overall size range = 128:1. The circles increase by 2.

Note: Both graticules would have to be used with the same microscope, eyepiece and objective.

Pattern Description Dia Cat # Qty Price  
NG5 Fairs, grid length 4 mm.
Glass Sandwich.
16 mm 68033-16 each 224.00 Add to Cart
19 mm 68033-19 each 224.00 Add to Cart
21 mm 68033-21 each 224.00 Add to Cart

arrow12E. Asbestos Fiber Analysis

Walton and Beckett Graticule for Asbestos - G22Walton and Beckett Graticule for Asbestos - G24Walton and Beckett Graticule for Asbestos - G25arrow10arrow10Walton and Beckett Graticule for Asbestos - G22, G24, G25

Calibration factors are required for each of these graticules. See note below.

The Walton and Beckett graticule is used for counting fibrous dust (e.g. asbestos or glass fibers) and is particularly useful where the majority of fibers to be counted are shorter than 5 microns. The circle is divided into four by two diametrical lines scaled in units of 5 and 3 microns respectively. 3 and 5 microns are the critical measurements of fiber lengths and diameters used in fiber counting. Unlike the usual globes of other particle graticules, the Walton and Beckett have a series of shapes to compare objects with. These shapes have been designed for comparison with fibers, especially since they incorporate an aspect ratio of 3:1 or 5:1, essential for such analysis.

Important Note: The circle on these Walton & Beckett graticules must represent 100 microns at the stage and each one must be manufactured to suit the individual instrument.

Therefore, the following details should be provided with your order:

  1. Calibration factor, if known
  2. Objective magnification
  3. Eyepiece magnification
  4. Diameter of graticule disc required
  5. Microscope make and model
Pattern Description Dia Cat # Qty Price  
G22 Walton & Beckett for asbestos, 3:1 ratio.
Glass sandwich.
16 mm 68028-16 each 272.00 Add to Cart
19 mm 68028-19 each 272.00 Add to Cart
20.4 mm 68028-20.4 each 272.00 Add to Cart
21 mm 68028-21 each 272.00 Add to Cart
23 mm 68028-23 each 272.00 Add to Cart
24.5 mm 68028-24.5 each 272.00 Add to Cart
25 mm 68028-25 each 272.00 Add to Cart
26 mm 68028-26 each 272.00 Add to Cart
27 mm 68028-27 each 272.00 Add to Cart
G24 Walton & Beckett for asbestos, 5:1 ratio.
Glass sandwich.
16 mm 68029-16 each 272.00 Add to Cart
19 mm 68029-19 each 272.00 Add to Cart
21 mm 68029-21 each 272.00 Add to Cart
G25 Based on the G22, the G25 is produced by the Institute of Occupational Health (1996).
Glass sandwich.
16 mm 68030-16 each 272.00 Add to Cart
19 mm 68030-19 each 272.00 Add to Cart
21 mm 68030-21 each 272.00 Add to Cart

arrow12F. Specialist Designs

Spray Droplet Sizing Reticle (Matthews), NG 30arrow10Spray Droplet Sizing Reticle (Matthews) - NG30

For size and distribution assessments of aerosol droplets. Used in conjunction with a 40x Microscope for direct measurements of droplets from 50-400 micron diameters. The actual pattern sizes are 50, 100, 200, and 400 microns. W.H.O. (Details on Request) and G.A. Matthews. Imperial College.

Pattern Description Dia Cat # Qty Price  
NG30 Matthews Spray Droplet 16 mm 68003-16 each 244.00 Add to Cart
19 mm 68003-19 each 244.00 Add to Cart
21 mm 68003-21 each 244.00 Add to Cart
23 mm 68003-23 each 244.00 Add to Cart
Custom 68003-50 each 310.00 Add to Cart
Thompson - G23arrow10Thompson - G23

For counting particles in any of three areas of known size. The graticule is calibrated in the same manner as a normal eyepiece scale. The result is then used to calculate the area of any square.

Pattern Description Dia Cat # Qty Price  
G23 Thompson for dust analysis.
10 mm, 7 mm and 4 mm squares.
Glass Sandwich.
16 mm 68090-16 each 275.00 Add to Cart
19 mm 68090-19 each 275.00 Add to Cart
21 mm 68090-21 each 275.00 Add to Cart
Chalkley Point Array - NG52arrow10Chalkley Point Array - NG52

This is used to quickly determine the relationship of components to each other using random sampling. Curtis gives an example of its application, where a researcher may want to see whether or not a certain drug affects the volume proportion of cell types in a given organ. With this graticule the proportion of points lying over the image of one type of component is statistically proportional to the area occupied by that component. The 25 points of the array are placed over the field of view at random, so that a comparison can be made between the number of points touching one type of component with the number touching the other type of component in each viewing. A series of observations will yield an increasingly accurate ratio of the comparative incidence of each type of particle.

Reference

  • A.S.C. Curtis. Medical and Biological Illustration, Vol. 10. pp 261-266. "Area and Volume Measurements by Random Sampling Methods."
Pattern Description Dia Cat # Qty Price  
NG52 Chalkley point array.
Surface chrome image.
16 mm 68034-16 each 242.00 Add to Cart
19 mm 68034-19 each 242.00 Add to Cart
21 mm 68034-21 each 242.00 Add to Cart
Pharmaceutical PSA Pattern - G57arrow10Pharmaceutical PSA Pattern - G57

This graticule was designed for the pharmaceutical industry. However, it is also useful where particle size considerations are restricted to 10 mm and 25 mm. Dots and circles give quick references for these two sizes. In addition, a scale is incorporated.

The microscope must be calibrated when ordering this graticule, such that the circle must equate to 1 mm on the microscope stage.

Reference

  • The United State Pharmaceutical Conventions, Inc. Pharmaceutical Forum. Vol. 19 No 6.
Pattern Description Dia Cat # Qty Price  
G57 Pharmaceutical PSA Pattern.
Glass Sandwich.
19 mm 68091-19 each 275.00 Add to Cart
20.4 mm 68091-20.4 each 275.00 Add to Cart
21 mm 68091-21 each 275.00 Add to Cart
23 mm 68091-23 each 275.00 Add to Cart
24.5 mm 68091-24.5 each 280.00 Add to Cart
25 mm 68091-25 each 280.00 Add to Cart
26 mm 68091-26 each 280.00 Add to Cart
27 mm 68091-27 each 280.00 Add to Cart
Counting Pattern - NG14arrow10Counting Pattern - NG14

Simple counting for geological and soil analysis.

Reference

  • L.G. Briarty. "Stereology: Methods for Quantitative Light and Electron Microscopy." Sci. Prog. Oxf. 1975 62; 1-32.
Pattern Description Dia Cat # Qty Price  
NG14 Counting pattern for soil analysis.
10 mm square.
Surface chrome image.
16 mm 68092-16 each 232.00 Add to Cart
19 mm 68092-19 each 232.00 Add to Cart
21 mm 68092-21 each 232.00 Add to Cart
Lennox Grain Analysis - NG21arrow10Lennox Grain Analysis - NG21
Pattern Description Dia Cat # Qty Price  
NG21 Lennox for Grain analysis. 16 mm 68004-16 each 262.00 Add to Cart
19 mm 68004-19 each 262.00 Add to Cart
21 mm 68004-21 each 262.00 Add to Cart
23 mm 68004-23 each 262.00 Add to Cart
Custom 68004-50 each 348.00 Add to Cart
Whipple Grid - NE29arrow10Kotter Pattern - G48

For analysis of bitumous coal and anthracite.

Reference

  • I.S.O. 7404-4: 1988 (E). Methods for Analysis of Bitumous Coal and Anthracite. Part 4 and Methods of Determining Microlithotype Composition.

Normally used with 20x objective = calibration factor of 1. For use with 40x objective, specify calibration factor of 2. For 50x specify 2.5. For other objective magnifications, the reticle will need to be custom made.

Pattern Description Dia Cat # Qty Price  
G48 Kotter Pattern for bitumous coal and anthracite analysis. 16 mm 67990-16 each 250.00 Add to Cart
19 mm 67990-19 each 250.00 Add to Cart
20.4 mm 67990-20.4 each 250.00 Add to Cart
21 mm 67990-21 each 250.00 Add to Cart
23 mm 67990-23 each 250.00 Add to Cart
24 mm 67990-24 each 302.00 Add to Cart
24.5 mm 67990-24.5 each 302.00 Add to Cart
25 mm 67990-25 each 302.00 Add to Cart
26 mm 67990-26 each 302.00 Add to Cart
27 mm 67990-27 each 302.00 Add to Cart
Custom 67990-50 each 340.00 Add to Cart
Zeiss Integrating Eyepiece Disc 100 Points — G47arrow10Zeiss Integrating Eyepiece Disc 100 Points - G47

Similar to G49, but extended to 100 points, which are indexed.

Pattern Description Dia Cat # Qty Price  
G47 Zeiss integrating eyepiece disc.
Glass sandwich.
16 mm 68094-16 each 273.00 Add to Cart
19 mm 68094-19 each 273.00 Add to Cart
21 mm 68094-21 each 273.00 Add to Cart
arrow10Zeiss Integrating Disc 1 or Henning Reseau Pattern 25 Points - G49
Zeiss Integrating Disc 1 or Henning Reseau Pattern 25 Points - G49

Reference

  • Zeiss Werkzeitschrft. No 30, page 80.
Pattern Description Dia Cat # Qty Price  
G49 Henning Resseau pattern (Zeiss integrating disc 1).
Glass sandwich.
16 mm 68093-16 each 26700 Add to Cart
19 mm 68093-19 each 26700 Add to Cart
21 mm 68093-21 each 26700 Add to Cart
Integrating EyePiece - G50arrow10Integrating EyePiece - G50
Pattern Description Dia Cat # Qty Price  
G50 Integrated EyePiece (Simplified). 19 mm 68005-19 each 240.00 Add to Cart
21 mm 68005-21 each 240.00 Add to Cart
23 mm 68005-23 each 253.00 Add to Cart
24 mm 68005-24 each 280.00 Add to Cart
Custom 68005-50 each 335.00 Add to Cart

arrow12G. Stereology

In this simplest form, sterology is the science where information about a three dimensional object is obtained from only a two-dimensional section of that structure.

Measurements are usually made with these graticules in the following manner:

  1. An adequate representation of sections of a specimen is obtained.
  2. The graticule is superimposed upon the specimen (or micrograph/projected image of the section).
  3. Finally, the interaction between the superimposed graticule and the test sections are recorded.

An overall introduction is given by: L.B. Brianrty. "Stereology: Methods for Quantitative Light and Electron Microscopy."

The Mertz Graticule (36 Points) - NGM1arrow10The Mertz Graticule (36 Points) - NGM1

Used to estimate the three-dimensional surface areas or the surface density of a component in a given volume, when the component does not have a random orientation. It comprises a test system with parallel curved lines used for measuring the intersection of points.

Reference

  • W.A. Mertz. "Mikroskopic" Vol. 22 1967 pp 132-142.
Pattern Description Dia Cat # Qty Price  
NGM1 Mertz for Stereology.
Surface chrome image.
16 mm 68035-16 each 242.00 Add to Cart
19 mm 68035-19 each 242.00 Add to Cart
21 mm 68035-21 each 242.00 Add to Cart
Weibel 1 - NGW1arrow10Weibel 1 - NGW1

Consists of 15 lines of equal length connecting the verticals of a regular hexagonal point network.

Reference

  • E.R.Weibel Lab. Invest. Vol. 22 pp 131-152. "Principal and Methods for the Morphometric Study of the Lung and other Organs."
Pattern Description Dia Cat # Qty Price  
NGW1 Weibel Type 1 for Stereology.
Surface chrome image.
16 mm 68022-16 each 242.00 Add to Cart
19 mm 68022-19 each 242.00 Add to Cart
21 mm 68022-21 each 242.00 Add to Cart
Weibel 2 - NGW2arrow10Weibel 2 - NGW2

Used when making a surface to volume ratio of a structure per mass unit. This graticule consists of a number of short lines with interruptions as long as the lines. Basically, the number of intersections falling over the short lines is counted and the number of endpoints falling on the end of the structure is determined.

Reference

  • E.R. Weibel, Journal of Microscopy Vol. 95. pp 373-378. Current Capabilities and Limitations of Available Sterrological Technique, point counting method.
Pattern Description Dia Cat # Qty Price  
NGW2 Weibel Type 2 for Stereology.
Surface chrome image.
16 mm 68025-16 each 242.00 Add to Cart
19 mm 68025-19 each 242.00 Add to Cart
21 mm 68025-21 each 242.00 Add to Cart
Weibel 3 - GW3arrow10Weibel 3 - GW3

Reference

  • E.R. Weibel, G.S. Kishtler & W.F. Scherle. 1986. J. Cell Biology. 30, 23.
Pattern Description Dia Cat # Qty Price  
NGW3 Weibel Type 3 for Stereology.
Glass sandwich.
16 mm 68023-16 each 242.00 Add to Cart
19 mm 68023-19 each 242.00 Add to Cart
21 mm 68023-21 each 242.00 Add to Cart

arrow12H. Metallurgy

Standard pattern discs for metallurgical stereometric analysis of grain size in polished metal sections.

arrow10Grain Sizing Pattern EN10247/ISO4976
Grain Sizing Pattern ISO4976Grain Sizing Pattern EN10247

For the determination of non-metallic inclusion content of steel.

NG60 meets EN 10247 and NG61 Meets ISO 4976. Both are scaled for use eith 10x objective magnification Pattern

Pattern Description Dia Cat # Qty Price  
NG60 Grain Sizing reticle to EN 10247. 21 mm 68007-21 each 254.00 Add to Cart
23 mm 68007-23 each 254.00 Add to Cart
24.5 mm 68007-24 each 295.00 Add to Cart
25 mm 68007-25 each 295.00 Add to Cart
26 mm 68007-26 each 295.00 Add to Cart
27 mm 68007-27 each 295.00 Add to Cart
Custom 68007-50 each 335.00 Add to Cart
NG61 Grain Sizing reticle to ISO 4967. 21 mm 68036-21 each 254.00 Add to Cart
23 mm 68036-23 each 254.00 Add to Cart
24.5 mm 68036-24 each 295.00 Add to Cart
25 mm 68036-25 each 295.00 Add to Cart
26 mm 68036-26 each 295.00 Add to Cart
27 mm 68036-27 each 295.00 Add to Cart
Custom 68036-50 each 330.00 Add to Cart
ASTM Austenite 1:1 Grain Sizing Disc - G41arrow10ASTM Austenite 1:1 Grain Sizing Disc - G41

Reference

  • VDEH 1510-61.
Pattern Description Dia Cat # Qty Price  
G41 ASTM Grain Size Austenite.
Glass sandwich.
19 mm 68065-19 each 280.00 Add to Cart
21 mm 68065-21 each 280.00 Add to Cart
ASTM E112 Plate 1 Grain Sizing Disc - G42arrow10ASTM E112 Plate 1 Grain Sizing Disc - G42
Pattern Description Dia Cat # Qty Price  
G42 Grain Sizing (E112).
Glass Sandwich.
19 mm 68066-19 each 262.00 Add to Cart
21 mm 68066-21 each 262.00 Add to Cart
ASTM Carbide Grain Sizing Chart - G43arrow10ASTM Carbide Grain Sizing Chart - G43
Pattern Description Dia Cat # Qty Price  
G43 ASTM Grain Sizing carbide.
Glass sandwich.
19 mm 68067-19 each 262.00 Add to Cart
21 mm 68067-21 each 262.00 Add to Cart
ASTM E45 - G44arrow10ASTM E45 - G44

Reference

  • ASTM E45.
Pattern Description Dia Cat # Qty Price  
G44 ASTM E45, Root 2 sides.
7.1 mm square, 10 mm scale.
19 mm 68068-19 each 262.00 Add to Cart
21 mm 68068-21 each 262.00 Add to Cart
ASTM E19-46 Grain Sizing Disc - G45arrow10ASTM E19-46 Grain Sizing Disc - G45
Pattern Description Dia Cat # Qty Price  
G45 ASTM Grain Sizing E19-46.
Glass sandwich.
19 mm 68069-19 each 262.00 Add to Cart
21 mm 68069-21 each 262.00 Add to Cart
ASTM E19-46 Grain Sizing Disc Root 2 - G46arrow10ASTM E19-46 Grain Sizing Disc Root 2 - G46
Pattern Description Dia Cat # Qty Price  
G46 ASTM Grain sizing E19-46 Root 2
Glass sanwich
19 mm 68070-19 each 262.00 Add to Cart
21 mm 68070-21 each 262.00 Add to Cart
Circular Grid ASTM 24 Points - G54arrow10Circular Grid ASTM 24 Points - G54

Reference

  • ASTM G562.
Pattern Description Dia Cat # Qty Price  
G54 ASTM 24 Point Circular Grid.
Glass sandwich.
16 mm 68084-16 each 270.00 Add to Cart
19 mm 68084-19 each 270.00 Add to Cart
21 mm 68084-21 each 270.00 Add to Cart
Square Grid ASTM 25 Points - G55
arrow10Square Grid ASTM 25 Points - G55

Reference

  • ASTM G562.
Pattern Description Dia Cat # Qty Price  
G55 ASTM 25 Point Square Grid.
Glass sandwich.
16 mm 68085-16 each 270.00 Add to Cart
19 mm 68085-19 each 270.00 Add to Cart
21 mm 68085-21 each 270.00 Add to Cart

Stage Graticules arrow13arrow13