Microscope Graticles and Stage Micrometers
EMS is proud to add to our already extensive line -- a new wide range of Microscopy Calibration Standards and Standard Graticules, which are manufactured in the USA . This new line adheres to extremely tight tolerances and all of them are available with or without Secondary or Direct NIST certification.
A. Linear Reference Scales
We offer Chrome-on-Glass (G) and Chrome-on-Opal glass (O) or Chrome-on Quartz (Q). The Opal and Quartz are offered as a special only and must be requested.
P: positive image (mostly clear or white); N: negative image (mostly opaque or black)
All EMS micrometers come in a sturdy wooden box.
Model SM-1
Glass Size: | 2” x 5.25” (50 mm x 132 mm) |
Scale Length: | 5 inch (English) X-axis only 125 mm (Metric) X-axis only |
Divisions: | English = 0.01”; Metric = 100µm (0.1mm) With numerical labeling every 1 mm and 0.1” |
60210-1PG | Stage Micrometer, SM-1, chrome on glass | each | 525.00 | Add to Cart |
60210-1PGC | Stage Micrometer, SM-1 with Calibration | each | 1,280.00 | Add to Cart |
Model SM-3
Glass Size: | 1” x 3” (25mm x 75mm). ≥1.42 mm (0.056”) thickness |
Scale Length: | 0.04 inch (English) X & Y axis 10 mm (Metric) X &Y axis |
Divisions: | English = 0.0005”; Metric = 10µm (0.010mm) With numerical labeling every 0.1 mm and 0.05” |
Flatness: | ≤ 10 µm / 100 mm |
Image Forming Material: | Chrome |
Image Contrast/Density: | Chrome transmission density is ≥ 2.0 |
Feature Size Accuracy: | 2 µm |
Image Placement Accuracy: | 0.001 mm / 100 mm |
Polarity: | Scales are opaque on a clear background |
Reading Direction: | Right Read Chrome Up (RRCU) |
Typical Uses: | SM-3 is useful for calibration of magnification in microscopes and other optical magnification systems. Its auto-location features can be used for testing of an image analysis auto-location system. |
60210-3PG | Stage Micrometer, SM-3, chrome on glass | each | 340.00 | Add to Cart |
60210-3PGC | Stage Micrometer, SM-3 with Calibration | each | 1,295.00 | Add to Cart |
Model SM-4
Glass Size: | 1” x 3” (25mm x 75mm) |
Scale Length: | 1 inch (English) X-axis only 25 mm (Metric) X-axis only |
Divisions: | English = 0.0005”; Metric = 10µm (0.010 mm) With numerical labeling every 0.1 mm and 0.05” |
60210-4PG | Stage Micrometer, SM-4, Chrome on glass | each | 320.00 | Add to Cart |
60210-4PGC | Stage Micrometer, SM-4 with Calibration | each | 1,200.00 | Add to Cart |
Model SM-5
Glass Size: | 1.5” x 3” (38mm x 75mm) |
Scale Length: | 1 inch (English) X & Y axis 25 mm (Metric) X & Y axis |
Divisions: | English = 0.001”; Metric = 25µm (0.025 mm) With numerical labeling every 1 mm and 0.1” |
60210-5PG | Stage Micrometer, SM-5, Chrome on glass | each | 479.00 | Add to Cart |
60210-5PGC | Stage Micrometer, SM-5 with Calibration | each | 1,100.00 | Add to Cart |
Model SM-6
Same scale as SM-5, but smaller division.
Glass Size: | 1.5” x 3” (38mm x 75mm). |
Scale Length: | 1 inch (English) X & Y axis 25 mm (Metric) X & Y axis |
Divisions: | English = 0.0005”; Metric = 10µm (0.010 mm) With numerical labeling every 0.1 mm and 0.05” |
60210-6PG | Stage Micrometer, SM-6, Chrome on Glass | each | 560.00 | Add to Cart |
60210-6PGC | Stage Micrometer, SM-6 with Calibration | each | 1,300.00 | Add to Cart |

Model SM-12
Glass Size: | 1” x 3” (25mm x 75mm). ≥1.42 mm (0.056”) thickness |
Scale Length: | 0.08 inch (English) X-axis only 2.0 mm (Metric) X-axis only |
Divisions: | English = 0.001”; Metric = 25µm (0.025 mm) No numerical scale labeling |
Flatness: | ≤ 10 µm / 100 mm |
Image Forming Material: | Chrome |
Image Contrast/Density: | Chrome transmission density is ≥ 2.0 |
Feature Size Accuracy: | 2 µm |
Image Placement Accuracy | 0.001 mm / 100 mm |
Polarity | Scales are opaque on a clear background |
Reading Direction | Right Read Chrome Up (RRCU) |
Typical Uses | SM-12 is useful for calibration of magnification in microscopes and other optical magnification systems. |
60210-12PG | Stage Micrometer, SM-12, chrome on glass | each | 230.00 | Add to Cart |
60210-12PGC | Stage Micrometer, SM-12 with Calibration | each | 750.00 | Add to Cart |
Model SM-13
Glass Size: | 1” x 3” (25mm x 75mm) |
Scale Length: | 0.08 inch (English) X & Y axis 2.0 mm (Metric) X & Y axis |
Divisions: | English = 0.001”; Metric = 25µm (0.025 mm) No numerical scale labeling |
60210-13PG | Stage Micrometer, SM-13, Chrome on glass | each | 295.00 | Add to Cart |
60210-13PGC | Stage Micrometer, SM-13 with Calibration | each | 900.00 | Add to Cart |
B. Image Analysis & Microscopy (IAM)
Vision Calibration Standards
We are proud to introduce the first complete and true line of Image Analysis Microscopy Calibration Standards, specially designed to address the current needs of the new generation of image analysis and machine vision systems. These unique standards are designed to test multiple morphometric parameters to assure that your image analysis system is working properly; meeting the required calibration and certification requirements of your vision measurement systems.
We offer Chrome-on-Glass (G) and Chrome-on-Opal glass (O) or Chrome on Quartz (Q). The Opal and Quartz are offered by request only as a custom.
P: positive image (mostly clear or white); N: negative image (mostly opaque or black)
Model IAM-1 – Morphometric Image Analysis Standards
Ideally for morphological calibration where two dimensional shapes have to be measured precisely. Standard includes a set of eight test plates of various shapes, positions, angles and sizes. It also includes a linear measurement scale for calibrating your imaging system. Overall size: 1” x 3” (25mm x 75mm). Positive image / Chrome on glass (PG)
Plate | Frame Size | Description |
1 | 4600 x 3500µm | Circles – 2000, 1000, 500 & 250 µm diameters |
2 | 1000 x 800 µm | Circles – 500, 250, 125 & 62.5 µm diameters |
3 | 1000 x 800 µm | Bars – 200x20 µm at 30 degrees increments |
4 | 1000 x 800 µm | Various Shapes, Circles, Bars & Angular designs. |
5 | 1000 x 800 µm | Squares – 100, 40, 20 µm set of 2 each. |
6 | 2050 x 1650 µm | Grid Patterns – 50 µm lines at 100 µm pitch |
7 | 4200 x 3400 µm | Grid Patterns – 200 µm lines at 200 µm pitch |
8 | 10 mm long | Scale with 10 µm divisions |
60216-1PG | Morphology Stage Micrometer, Model IAM-1 | each | 425.00 | Add to Cart |
60216-1PGC | Morphology Stage Micrometer, Model IAM-1, T/L, Calibrated only plates 1, 2 & 8 | each | 1,075.00 | Add to Cart |
Model IAM-MET
ASTM Grain Size / Nodularity Analysis Standard
Based on the IAM-1 Morphometric Image Analysis above, the IAM-MET is a multi-morphology calibration standard designed specifically for ASTM. The IAM-MET includes all plates as found in IAM-1 except for plate 4, which is pictured above. This plate contains 9 images of irregular shapes, simulating features commonly found in metallurgical applications. As required by the ASTM standard, the IAM-MET includes secondary certification of plates 1, 2 and 8.
Overall size: | 76.2 mm x 25.4 mm (3” x 1”) |
Image: | Chrome on glass / positive. Calibrated 32 points. |
60216-METC | Model IAM-MET, ASTM Grain Size, 32 pts Calibrated | each | 699.00 | Add to Cart |
Model IAM-2 – Linear (X & Y axis) Scale
A dual axis linear micrometer that can accurately calibrate both X & Y directions without rotation, for optical magnification, and camera aspect ratio using single standard. In addition, it can be used to calibrate and check linear stage motion or related auto measuring systems. Scales are in both English and Metric measurements.
Overall size: | 1” x 3” (25 mm x 75 mm) |
Scale Image: | 1” (right scale); 25 mm (left scale) Y = 0.60” (right scale); 15 mm (left scale) |
Divisions: | English = 0.001”, Metric = 25µm |
Image: | Positive image / Chrome on glass (PG) |
60216-2PG | Linear (X&Y axis) Stage Micrometer, Model IAM-2 | each | 325.00 | Add to Cart |
60216-2PGC | Linear (X&Y axis) Stage Micrometer, Model IAM-2, T/L, Calibrated | each | 1,100.00 | Add to Cart |
Model IAM-5 – Stage Mapping – Grid Pattern
This ideal standard is for verifying or qualifying multiple image analysis parameters such as optical distortion, alignment between systems or optical paths, and cell areas for particle counting. The image area is 20mm x 50mm.
Overall size: | 1” x 3” (25mm x 75mm) |
Image Forming Material: | Negative image / Chrome on glass (NG) |
Model No. | Clear Aperture | Opaque Lines | Pitch |
IAM-5S | 8.5 µm square | 1.5 µm square | 10 µm |
IAM-5M | 180 µm square | 20 µm square | 200 µm |
IAM-5L | 980 µm square | 20 µm square | 1000 µm |
60216-5SNG | Model IAM-5S, Grids, Small Size, 10µm Pitch | each | 525.00 | Add to Cart |
60216-5SNGC | Model IAM-5S with Calibrated (12 pts) | each | 1,040.00 | Add to Cart |
60216-5MNG | Model IAM-5M, Grids, Medium Size, 200µm Pitch | each | 425.00 | Add to Cart |
60216-5MNGC | Model IAM-5M with Calibrated (12 pts) | each | 950.00 | Add to Cart |
60216-5LNG | Model IAM-5L, Grids, Large Size, 1000µm Pitch | each | 295.00 | Add to Cart |
60216-5LNGC | Model IAM-5L with Calibrated (12 pts) | each | 875.00 | Add to Cart |
Model IAM-8 – Multi Grid Standard
A uniqulye designed standard with 4 different grid size patterns: 0.5 x 0.5 mm grid with 25 µm pitch; 1.0 x 1.0 mm grid with 50 µm pitch; 2.0 x 2.0 grid with 100 µm pith; and 4.0 x 4.0 mm grid with 200 µm pitch. Also includes a Star Target with 15 degree angle increments. This standard can be used to test not only the overall frame distortion issue, but also linear distances for a wide variety of magnification ranges from 1x to 1000x power.
Overall Size | 1” x 3” (25mm x 75mm) |
Image: | Positive image / Chrome on glass (PG) |
60216-8PG | Model IAM-8, Multi Grids Standard | each | 250.00 | Add to Cart |
60216-8PGC | Model IAM-8 Calibrated (16 pts) | each | 600.00 | Add to Cart |
ACM-1 – Calibration Micrometer
This ideal standard provides accurate calibration of any eyepiece, reticules, filar micrometers, or electronic measuring instrument. Features a series of ‘H’ shaped fiducial images in a range of sizes from 0.1 mm through 20 mm, useful for calibration range from 1X to 1000X power.
Standard Size: | 25 mm x 75 mm | ||||||||||||
Substrate Type: | Transparent glass (G), 1.42 mm or thicker Opal glass (O) is available per request. |
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Image Forming Material: | Evaporated chromium on glass | ||||||||||||
Image Description: | ‘H’ shaped fiducials as follows, centerline to centerline
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Polarity: | Positive (opaque or black features on clear background) | ||||||||||||
Reading Direction: | Right Read Chrome Up (RRCU) | ||||||||||||
Image Placement Accuracy: | 3.0 microns or better | ||||||||||||
Image Contrast/Density: | Chrome optical density grater than 2.0 | ||||||||||||
Typical Use: | Verification or calibration of magnification. |
60218-1PG | Model ACM-1, Calibrating Micrometer | each | 310.00 | Add to Cart |
60218-1PGC | Model ACM-1, with 11 pts Calibrated | each | 600.00 | Add to Cart |
C. Color Transmission Calibration
NIST Traceable Color Transmission Calibration Slide - Calibrated

Microscopy color patches for assessing color accuracy. NIST traceable calibration data for each large color patch is supplied as spectral transmission. By using the supplied spreadsheet, this data can be converted to the color space you need (such as Adobe RGB 1998, L*A*B*, XYZ, etc.)
-24 color patches plus large clear area
-Black background to minimize flare
-The 4150 µm diameter patches are individually calibrated for spectral transmission. This allows accurate conversion (using the supplied spreadsheet) to other color space units.
-Smaller patches from 1000 µm diameter to 150 µm diameter are exposed and processed at the same time produce good correlation of the large calibrated patches to the smaller ones, but are not calibrated.
-Oil immersion objectives can be used because a standard 0.15 mm glass cover slip is permanently cemented over the color film.
-Film is permanently sandwiched between glass; atmospheric deterioration of the measured values is minimized.
-Each slide is individually serialized and calibrated.
Specifications
Overall Size | 75mm x 25mm |
Feature Array | 24 unique color patches in 9 different sizes, ranging from 4.150 mm diameter to 0.150 mm diameter, and a large clear film area. Only the large patches (and clear area) are directly calibrated, but all patches are imaged and processed at the same time. |
Polarity | Negative |
Material | 0.2 mm (0.007") thick polyester photo-film mounted onto 0.9 to 1 mm water white Soda-lime Glass with a 0.15 mm (0.006") glass coverslip for protection (exact thickness is subject to change) |
Includes
- Calibrated IAM-9C color microscope slide
- Calibration Certificate
- Calibration Data Report
- Conversion Spreadsheet
- USB flash drive with data and spreadsheet
- Protective case
79504 | NIST Traceable Color Transmission Calibration Slide - Calibrated | each | 1,250.00 | Add to Cart |