Cell Culture: Coverglass
CultureWell™ Removable Chambered Coverglass
CultureWell™ with removable chambered coverglass – ideal for cell culture and fluorescence imaging applications
The 2 x 8, 16 chambered coverglass is like a chambered slide with the imaging advantages of a removable coverglass substructure. It is designed with standard 96 well plate volumes and well spacing. The non-cytotoxic silicone well gasket forms a leak-proof seal between the polystyrene upper structure and the coverglass. CultureWell™ Chambered Coverglass is shipped sterile and ready to use.
Features:
- The growth surface is No. 1.5 German borosilicate coverglass.
- Inert, non-cytotoxic silicone permits complete edge-to-edge growth of cells.
- Well spacing allows use of multi-channel pipettes for ease and speed of handling
- Cell growth characteristics are optimal due to the use of inert silicone materials and manufacturing process.
- Quality test for non-leakage, cell growth and coverglass removal to ensure consistent results
- Silicone well gasket remains attached to coverglass after separation, allowing wells to be used as reagent reservoirs
- Gasket well design is ideally suited for small volume incubations, in-situ hybridization and immunostaining
- Black well gasket reduces light scatter, enhancing fluorescence applications. The gasket can easily be removed from the coverglass for mounting
- Frosted microscope slides are provided for mounting
Key product dimensions include:
- Coverslip gasket thickness is 0.5 mm
- Interior diameter of each well is approx. 6 mm
- Volume per gasket well (upper structure removed) is approx. 9 µl
- Volume per well with the upper structure is 250 µl
Ordering:
| EMS # | Qty | Price | Select | Specification |
| 70459-08 | 8/pk | 200.00 | Add to Cart |  |
| 70459-08-T | each | 9.95 | Add to Cart | |
16 Well CultureWell™ Removeable Chambered Coverglass Spare CultureWell™ Coverglass Removal Tool, |
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CultureWell™ Chambered Coverglass
Chambered coverglass products are optimal for high resolution microscopy
Chambered coverglass products consists of removable / reusable non-cytotoxic silicone gaskets secured to 24 mm x 50 mm, #1.5 German coverglass. They may be placed in sterile culture plates or 100 mm diameter culture dishes.
Features:
- The thin flat coverglass is the optimal platform for high resolution microscopy and high-sensitivity, quantitative, fluorescent imaging
- Leak-proof design eliminates well-to-well cross contamination
- Gaskets stabilize coverglass and provide convenient handling using conventional microscope slide holders and accessories
- Gaskets are removable for high resolution imaging
- Gasket may be re-sterilized and reused
- Well spacing facilitates liquid handling utilizing multichannel pipettes and robotic dispensers.
Ordering:
| EMS # | Depth | Price | Select | Specification |
| 70460-2R1 | 1mm | 198.00 | Add to Cart |  |
| 70460-2R2 | 1.5mm | 219.00 | Add to Cart | |
| 2 Wells – 15mm Dia 250-400µl Volume (-2R1) 300-500µl Volume (-2R2) 20 per pack |
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| 70460-3S | 1mm | 198.00 | Add to Cart |  |
| 3 Wells – 9.5mm x 9.5mm ID 300-500µ Volume 20 per pack |
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| 70460-4R | 1mm | 198.00 | Add to Cart |  |
| 4 Wells – 9mm Dia 50-100µl Volume 20 per pack |
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| 70460-50R | 1mm | 198.00 | Add to Cart |  |
| 50 Wells – 3mm Dia 3-10µl Volume 20 per pack |
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| 70460-8R | 1mm | 198.00 | Add to Cart |  |
| 8 Wells – 6mm Dia 15-30µl Volume 20 per pack |
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CultureWell™ Chambered Coverglass Inserts
Each CultureWell™ insert is comprised of four chambered coverglass assembled in a disposable frame which is placed in a standard 86 mm x 128 mm culture plate. All are sterile and ready to use.
The stabilizing insert design and accessory hardware allow fragile coverglass to be easily integrated into protocols for high throughput handling of microscope slides and SBS compliant microtiter plates.
Ordering:
| EMS # | Wells | Dia | Volume | Depth | Qty | Price | Select |
| 70461-2R1 | 8 | 6mm | 300-500µ | 1mm | 10/pk | 648.00 | Add to Cart |
| 70461-2R2 | 8 | 6mm | 300-500µ | 1.5mm | 10/pk | 760.00 | Add to Cart |
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| 70461-3S | 3 Wells | 9.5mm x 9.5mm ID | 300-500µ | 1mm | 10/pk | 760.00 | Add to Cart |
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| 70461-4R | 4 Wells | 9mm | 50-100µ | 1mm | 10/pk | 995.00 | Add to Cart |
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| 70461-50R | 50 Wells | 3mm | 3-10µl | 1mm | 10/pk | 995.00 | Add to Cart |
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| 70461-8R | 8 Wells | 6mm | 15-30µl | 1mm | 10/pk | 995.00 | Add to Cart |
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SecureSlip™ Cell Culture Coverglass
Convenient, Secure coverglass that will not move during cultivation
The coverglass is affixed to a thin microscopically transparent silicone base which secure to culture vessels by an electrostatic charge, not an adhesive, to prevent movement during cultivation.
The silicone also acts as a hydrophobic barrier to isolate liquid reagents on glass. Individual coverglass with silicone backing can be adhered to a petri dish or plate. These can be easily removed one at a time from the dish during culture. Once removed, coverglass can be affixed to a microscope slide by means of the tacky silicone backing for routine processing, or placed cell side down onto a drop of reagent using the silicone backing as a cover to prevent evaporation.
Features:
- Silicone backing peels off easily for conventional mounting on microscope slides
- During culture, cells may be imaged without interference from the optical grade silicone
- Eliminates coverglass overlapping during culture. Handle SecureSlips™ with forceps by the silicone backing to avoid damage to glass or cells
- Easily identify "cell side" of coverglass
Ordering:
| 70462-12 | 378.00 | Add to Cart |  |
| 15 Well SecureSlip Silicone Supported Coverglass 12 mm x 12mm Coverglass 75 per pack |
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| 70462-14 | 378.00 | Add to Cart |  |
| 15 Well SecureSlip Silicone Supported Coverglass 12 mm Dia Coverglass 75 per pack |
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MultiSlip™ Cell Culture Coverglass Inserts
Sterile, ready-to-use MultiSlip™ for culture of cells where pretreatment with a biological coating of glass surface is required.
MultiSlip™ insert with 8 (18mm x 18mm) or 15 (12mm x 12mm) number 1.5 thickness German coverglass per insert are sterile and ready to use in conventional 86mm x 128mm culture plates.
Staining and washing procedures may be performed with MultiSlip™ inserts in the plate, or silicone backed coverglass may be removed individually and affixed to glass microscope slides. Alternatively, inserts may be easily removed for batch processing in glass staining dishes.
MultiSlips™ are ideally suited for the culture of cells where pretreatment of glass surfaces with a biological coating is required. Simply add sterile solution to the plate incubates and aspirate. Coating is applied evenly to one side of the glass only, with no over-lapping, handling with forceps.
Ordering:
| EMS # | Specification | Qty | Price | Select |
| 70463-12 | 15 – 12mm x 12mm Silicone Supported Coverglass Per Tray | 10/pk | 660.00 | Add to Cart |
| 70463-18 | 8 – 18mm x 18mm Silicone Supported Coverglass Per Tray | 10/pk | 690.00 | Add to Cart |
CultureWell™ Accessories
Reusable CultureWell™ gaskets from CultureWell™ Chambered Coverglass and CultureWell™ Chamber Coverglass Inserts are available bulk packaged.
- Gaskets are ideal for forming wells on glass microscope slides or in polystyrene dishes
- Gaskets are non-sterile and may be sterilized by autoclaving, UV or alcohol.
Ordering:
| EMS # | Depth | Price | Select | Specification |
| 70465-2R1 | 1mm | 72.00 | Add to Cart |  |
| 70465-2R2 | 1.5mm | 79.00 | Add to Cart | |
| Gasket Only 2 Wells – 15mm Dia 250-400µl Volume (-2R1) 300-500µl Volume (-2R2) 10 per pack |
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| 70465-3S | 1mm | 72.00 | Add to Cart |  |
| Gasket Only 3 Wells – 9.5mm x 9.5mm ID 300-500µ Volume 10 per pack |
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| 70465-4R | 1mm | 72.00 | Add to Cart |  |
| Gasket Only 4 Wells – 9mm Dia 50-100µl Volume 10 per pack |
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| 70465-8R | 1mm | 72.00 | Add to Cart |  |
| Gasket Only 8 Wells – 6mm Dia 15-30µl Volume 10 per pack |
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| 70465-50R | 1mm | 77.00 | Add to Cart |  |
| Gasket Only 50 Wells – 3mm Dia 3-10µl Volume 10 per pack |
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Additional Accessories:
| EMS # | Description | Pack | Price | |
| 70466-10 | 86mm x 128mm Polystyrene Culture Plate, Sterile | 10 | 31.00 | Add to Cart |
| 70466-20 | Stainless Steel Handles for Inserts | 5 | 55.00 | Add to Cart |
| 70466-30 | 24mm x 50mm, #1.5 Thickness German Coverglass | 100 | 85.00 | Add to Cart |
| 70466-40 | Reusable Silicone Well Dividers 2 - 18mm x 18mm Wells, 2.0mm Depth, Sterile | 4 | 76.00 | Add to Cart |
Protein Crystallization - Creative Tools for the Study of Cells
ProCrystal™ Protein Crystallization Covers: Hanging drop microtiter plate seals with unmatched drop formation and segregation and no noticeable x-ray diffraction
ProCrystal™ covers are designed for high throughput protein x-ray crystallography to generate atomic resolution structures of protein molecules1. They fit SBS compliant microtiter plates and are TTP Mosquito®*compatible. They are packed dust-free, ready-to-use in packs of 5 with either pre-cut or uncut seals.
The seals sit directly over the microplate crystallization wells. The seals are validated to be incorruptible and materials will not ingress into samples. They are available in either an individual well pre-cut format so that one can easily remove crystals using a forceps from individual wells without affecting neighboring wells or uncut format.
The plate seals are UV compatible with the highest optical quality available. They are exceptionally hydrophobic which maintains drop footprint and segregation even with the use of MPD, Glycerol or detergents.
Features:
- Two Formats: Individually cut & single sheet un-cut seals
- TTP LabTech Mosquito®* compatible
- Ready-to-use, dust-free
- UV Compatible
- No noticeable X-ray diffraction
- Accommodates up to 3 protein drops per well
References:
1Newby, et. al. A General Protocol for the Crystallization of Membrane Proteins for X-ray Structural Investigation. Nature Protocols, Vol. 4, 619-637 (April 1, 2009)
Ordering:
| EMS # | Type | Price | Select | Specification |
| 70470-10 | Paper | 54.00 | Add to Cart |  |
| 70470-20 | Plastic | 57.00 | Add to Cart | |
| PCC Uncut Liner 5 per pack |
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| 70470-60 | Paper | 69.00 | Add to Cart |  |
| 70470-70 | Plastic | 76.00 | Add to Cart | |
| PCC Cut Liner 5 per pack |
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References
CultureWell™ Products References
