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Electron Microscopy Sciences

Scanning Electron Microscope Supplies

arrow13NanoSuit® Aqueous Solution for SEM

Forms a thin, conductive, moisture barrier between specimens and a vacuum environment

Taking electron microscope images of biological objects in their natural state

 

What is the NanoSuit®

  • A novel technology which enables us to observe cells, microorganisms, etc. in a living state using scanning electron microscopy (SEM).
  • NanoSuit forms a very thin barrier layer on the surface of an object and the barrier layer holds moisture in the object under vacuum condition in electron microscopy. Also, the barrier layer is electrically conductive.
  • Therefore, NanoSuit makes it possible to observe biological objects with their natural image texture.
  • NanoSuit is very easy to use. Only dropping the NanoSuit solution onto the objects, then you can observe the objects using SEM. (You don't need any other fixation procedures.)

History

Inventor Professor Takahiko Hariyama tried to observe various microorganisms using SEM, but almost all organisms died due to losing moisture under the vacuum condition in the electron microscopy.

However, he discovered a kind of fly larva was able to keep alive under the vacuum condition.

Prof. Hariyama investigated that phenomenon and revealed that a very thin barrier layer was formed on the surface of the larva by electron beam irradiation. The barrier layer held moisture and kept the larva alive.

Hariyama and his colleagues had tried to mimic this phenomenon artificially and invented "NanoSuit" technology.

Science

The "NanoSuit"

  • Aqueous solution of the biocompatible polymer. (biologically safe material)
  • Just drop onto the objects.
  • Polymerize by electron beam or plasma irradiation, then a thin barrier layer is formed on the surface of the objects.
  • The barrier layer holds moisture contained in the objects and its conductivity provides clear SEM images.

Example (flower petal)

  Control  

Under the vacuum condition, the petals are dried and do not retain the original shape.

Cryo-SEM NanoSuit  
 
NanoSuit keeps the petal moist under the vacuum condition and provides natural shape images of the fine structure of the petal surface.  

Example (insect)

With NanoSuit, you can observe the minute structure of the insect's body surface with a simple operation.

Example (living tissues)

Nanosuit keeps the texture of living cells and enables to distinguish between cancer tissue and normal tissue.

Example (chicken drumsticks)

The surface of the chicken drumsticks (Fresh) is protected by NanoSuit solution and observed by FE-SEM with high resolution shows fibrous microstructures of uniform size.

On the other hand, in (Freeze-thaw) examined by the same method, it was observed that the fine structure of the surface was broken and liquid substances were seeping out to the surface.

These images suggest that such a difference in surface condition may be the cause of the dry feeling of the thawed food.

Example (CLEM; Correlative light electron microscopy)

Prepare a glass slide using the conventional method for an optical microscopy. When you drop the tiny NanoSuit solution on the sample, you can observe with an electron microscope. By using this NanoSuit-CLEM method, you can observe the exact the same place of the sample with an optical microscope and an electron microscope. Since metal deposition is not required, you can store your important samples on slide glass again for an optical microscope. A lot of information can be obtained by combining the stained optical microscope observation results with the three-dimensional, high-magnification electron microscope observation.

Example (pathological specimen)

Amyloid deposition of cardiac muscle tissue - NanoSuit CLEM Method
By CLEM method using NanoSuit, it is possible to make a diagnosis by combining amyloid deposition in an image stained with an optical microscope and changes in the surrounding tissue morphology observed using electron microscopy.

Example (pathological specimen - Nephritis)

It is observed that the glomerulus is broken about half due to inflammation and lymphocytes are infiltrated.

References

Kawasaki, H., Itoh, T., Takaku, Y. et al. The NanoSuit method: a novel histological approach for examining paraffin sections in a nondestructive manner by correlative light and electron microscopy. Lab Invest 100, 161-173 (2020). https://doi.org/10.1038/s41374-019-0309-7

Shinmura K, Kawasaki H, Baba S, et al. Utility of Scanning Electron Microscopy Elemental Analysis Using the 'NanoSuit' Correlative Light and Electron Microscopy Method in the Diagnosis of Lanthanum Phosphate Deposition in the Esophagogastroduodenal Mucosa. Diagnostics (Basel). 2019;10(1):1. Published 2019 Dec 18. doi:10.3390/diagnostics10010001

Takaku Yasuharu, Suzuki Hiroshi, Kawasaki Hideya, Ohta Isao, Ishii Daisuke, Hirakawa Satoshi, Tsutsui Takami, Matsumoto Haruko, Takehara Sayuri, Nakane Chinatsu, Sakaida Kana, Suzuki Chiaki, Muranaka Yoshinori, Kikuchi Hirotoshi, Konno Hiroyuki, Shimomura Masatsugu and Hariyama Takahiko 2017A modified 'NanoSuit®' preserves wet samples in high vacuum: direct observations on cells and tissues in field-emission scanning electron microscopyR. Soc. open sci.4160887.

Storage Conditions

Short Term: Refrigerator, 4°C
Long Term: Frozen, -10°C

Ordering Table

11111-01 NanoSuit Solution I
for micro-organisms/individuals/living tissues
5ml 995.00 Add to Cart
11111-02

NanoSuit Solution II
for CLEM

    Coming Soon

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