Bismuth Stain Kit for Specific En Bloc Staining
Bismuth Stain Kits for Specific En Bloc Staining Kit consists of:
- 10x5ml Solution A (Sodium Tartrate, NaOH and Bismuth Subnitrate)
- 10x10ml Solution B (.2M Triethanolamine-HCl buffer, pH 7.0)
Mix Solution A and B together (One part of A into two parts of B) then adjust to pH 7.0 with 1N HCl.
Aldehyde-fixed tissue blocks are rinsed in the above buffer and stained en-bloc with Bismuth for one hour at room temperature on a gentle rotary shaker. Postfixation is carried out with 1% Osmium Tetroxide in 0.05M Sodium Cacodylate buffer, pH 7.2.
Post-staining is not needed.
Locke & Huie, (1977). Bismuth staining for Light and Electron Microscopy. Tissue Cell 9, 347.