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Electron Microscopy Sciences

Technical Data Sheets

Bismuth Stain Kit for Specific En Bloc Staining

EMS #11430

Bismuth Stain Kits for Specific En Bloc Staining Kit consists of:

  • 10x5ml Solution A (Sodium Tartrate, NaOH and Bismuth Subnitrate)
  • 10x10ml Solution B (.2M Triethanolamine-HCl buffer, pH 7.0)

Procedures

Staining solution:

Mix Solution A and B together (One part of A into two parts of B) then adjust to pH 7.0 with 1N HCl.

Aldehyde-fixed tissue blocks are rinsed in the above buffer and stained en-bloc with Bismuth for one hour at room temperature on a gentle rotary shaker. Postfixation is carried out with 1% Osmium Tetroxide in 0.05M Sodium Cacodylate buffer, pH 7.2.

Post-staining is not needed.

Reference

Locke & Huie, (1977). Bismuth staining for Light and Electron Microscopy. Tissue Cell 9, 347.

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Bismuth Stain Kits for Specific En Bloc Staining