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Electron Microscopy Sciences

Technical Data Sheets

Fast Frozen Stain Kit

EMS #15285-00

A rapid method to stain frozen tissue for permanent use. This kit includes a unique fixative for frozen tissue that is alcohol based, and will not interfere with possible immunohistochemical procedures and suitable for Laser Capture Microdissection (LCM).

Kit Components:

  • Fast Fix Frozen Tissue Fixative
  • Gills Hematoxylin #3
  • Scott’s Bluing Reagent
  • Eosin Y 0.5%
  • Citramount™ Mounting Media

Procedure:

  1. Place frozen section slides in “Fast Fix” Frozen Tissue Fixative for 2-3 minutes.
  2. Rinse in distilled water.
  3. Place Slides in Gill’s Hematoxylin #3 for 3-5 minutes.
  4. Rinse in Distilled Water.
  5. Blue in Scotts Bluing Reagent 3-4 dips.
  6. Rinse in Distilled Water.
  7. Dip Gently in Eosin in 5-10 seconds.
  8. Dehydrate in two changes each of 95% alcohol and 100% alcohol
  9. Clear in Xylene and coverslip with Citramount™.

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Fast Frozen Stain Kit