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Electron Microscopy Sciences

Technical Data Sheets

Paraformaldehyde - Glutaraldehyde Solution
(Karnovsky's Fixative)

EMS Catalog #15720

Using Paraformaldehyde-Glutaraldehyde Solution (Karnovsky's Fixative)

Kit contains

  • 2 x 10 ml 16% Paraformaldehyde Solution
  • 1 x 10 ml 50% EM Grade Glutaraldehyde
  • 1 x 50 ml Sodium Phosphate Buffer .2M, pH 7.2

Mixture 1 (Original Karnovsky's Mixture)

16% Paraformaldehyde Solution:   20 ml
50% Glutaraldehyde EM Grade:   8 ml
0.2M Sodium Phosphate Buffer:   25 ml
Distilled Water:   25 ml

Final mixture is 78ml with 5% Glutaraldehyde, 4% Formaldehyde in 0.08M buffer. This mixture is extremely hypertonic, with an osmolarity of more than 2000m OSM .

Mixture 2

A lower concentraction of Glutaraldehyde and Formaldehyde especially used for vascular profusion.

16% Paraformaldehyde Solution:   13 ml
50% Glutaraldehyde EM Grade:   5 ml
0.2M Sodium Phosphate Buffer:   50 ml
Distilled Water:   32 ml

Final mixture is 2% Paraformaldehyde, 2.5% Glutaraldehyde and 0.1M Buffer. If necessary, the osmolarity can be adjusted by adding sucrose to NaCl. The pH may also need to be adjusted to your desirable point. This mixture is the most effective general fixative for both plant and animal specimens.

Product Information

Paraformaldehyde - Glutaraldehyde Solution (Karnovsky's Fixative)