Paraformaldehyde - Glutaraldehyde Solution (Karnovsky's Fixative)
EMS Catalog #15720
Using Paraformaldehyde-Glutaraldehyde Solution Karnovsky's Fixative
- 2 x 10ml 16% Paraformaldehyde Solution
- 1 x 10ml 50% EM Grade Glutaraldehyde
- 1 x 50ml Sodium Phosphate Buffer .2M, pH 7.2
Mixture 1: (Original Karnovsky's Mixture)
|16% Paraformaldehyde Solution:||20ml|
|50% Glutaraldehyde EM Grade:||8ml|
|0.2M Sodium Phosphate Buffer:||25ml|
Final mixture is 78ml with 5% Glutaraldehyde, 4% Formaldehyde in 0.08M buffer. This mixture is extremely hypertonic, with an osmolarity of more than 2000m OSM .
A lower concentraction of Glutaraldehyde and Formaldehyde especially used for vascular profusion.
|16% Paraformaldehyde Solution:||13ml|
|50% Glutaraldehyde EM Grade:||5ml|
|0.2M Sodium Phosphate Buffer:||50ml|
Final mixture is 2% Paraformaldehyde, 2.5% Glutaraldehyde and 0.1M Buffer. If necessary, the osmolarity can be adjusted by adding sucrose to NaCl. The pH may also need to be adjusted to your desirable point. This mixture is the most effective general fixative for both plant and animal specimens.