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Electron Microscopy Sciences

Technical Data Sheets

Paraformaldehyde - Glutaraldehyde Solution (Karnovsky's Fixative)

EMS Catalog #15720

Using Paraformaldehyde-Glutaraldehyde Solution Karnovsky's Fixative

Kit contains:

  • 2 x 10ml 16% Paraformaldehyde Solution
  • 1 x 10ml 50% EM Grade Glutaraldehyde
  • 1 x 50ml Sodium Phosphate Buffer .2M, pH 7.2

Mixture 1: (Original Karnovsky's Mixture)

16% Paraformaldehyde Solution:   20ml
50% Glutaraldehyde EM Grade:   8ml
0.2M Sodium Phosphate Buffer:   25ml
Distilled Water:   25ml

Final mixture is 78ml with 5% Glutaraldehyde, 4% Formaldehyde in 0.08M buffer. This mixture is extremely hypertonic, with an osmolarity of more than 2000m OSM .

Mixture 2:

A lower concentraction of Glutaraldehyde and Formaldehyde especially used for vascular profusion.

16% Paraformaldehyde Solution:   13ml
50% Glutaraldehyde EM Grade:   5ml
0.2M Sodium Phosphate Buffer:   50ml
Distilled Water:   32ml

Final mixture is 2% Paraformaldehyde, 2.5% Glutaraldehyde and 0.1M Buffer. If necessary, the osmolarity can be adjusted by adding sucrose to NaCl. The pH may also need to be adjusted to your desirable point. This mixture is the most effective general fixative for both plant and animal specimens.

Online Ordering

Paraformaldehyde - Glutaraldehyde Solution (Karnovsky's Fixative) is available online from the EMS Catalog. For ordering or product information, click here.