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Electron Microscopy Sciences

Technical Data Sheets

Dapi-Fluoromount-G™

EMS Catalog #17984-24

Description

Dapi-Fluoromount-G™ is a water-soluble, instant-blue nuclear probe fluorescing (455nm) compound for mounting fixed slides using a staining procedure having an aqueous final step. Mounting slides with Dapi-Fluoromount-G™ stains the cell nucleus and may also reduce fluorochrome quenching during analysis of slides by fluorescence microscopy. This mounting medium also provides a semi permanent seal for long-term storage of slide preparations.

Research Applications

  • Slide Mounting

Instructions For Use

  1. Prepare cytocentrifuge preps according to established procedures.
  2. Fix and rehydrate preps.
  3. Remove one slide at a time from aqueous buffer, add 1 drop of Dapi-Fluoromount-G™ directly to cel prep, mount cover slip and press gently with a gauze sponge to remove excess mounting medium and to seal the cover slip.
  4. Allow mounted preps to air-dry for 5 minutes before examination.

Handling And Storage:

Dapi-Fluoromount-G™ is supplied as a 20ml solution containing 0.1% NaN3 as preservative; store at room temperature. Avoid exposure to light.

Warning

Reagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potential explosive deposits in lead or copper plumbing.

For Research Use Only. Not For Diagnostic Or Therapeutic Use.

Online Ordering

Dapi-Fluoromount-G™ is available online from the EMS Catalog. For ordering or product information, click here.