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Electron Microscopy Sciences

Technical Data Sheets

Platinum Blue, an EM Stain

EMS Catalog #22407

Description

IBI Blue Platinum Stain Pt4N8H6O24C20

Preparation

  • Adjust pH of distilled water with concentrated acetic acid to 3.5
  • Dilute PtBlue stock solution as supplied 1:100 in pH-adjusted water

Use

  • Incubate grid/Epon section on a drop of PtBlue 1:100 for 5 min at RT
  • Wash on a water drop for about 2 min, repeat 5x
  • Dry grid/section for approx. 10 min in an incubator
  • Stain with lead citrate for 2 min at RT
  • Jet-wash grid 5x (water stream from a plastic pastette)
  • Suck up remaining water with a filter paper and dry thoroughly
Cartilage ECM, constrasted with PtBlue + LC   Cartilage ECM, constrasted with UA + LC
Cartilage ECM, constrasted with PtBlue + LC   Cartilage ECM, constrasted with UA + LC
HepG2 cells, constrasted with PtBlue + LC   Depending on the sample, PtBlue can be nearly as good as uranyl acetate in its ability to add contrast. It does not work well with chondrocytes or T. brucei in our hands.
HepG2 cells, constrasted with PtBlue + LC    

Credits

Schmidt, Katy. "Technical Note." IBI Labs. Web. 15 May 2015.

Product Information

Platinum Blue, an EM Stain