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Electron Microscopy Sciences

Technical Data Sheets

UranyLess – Protocols of Use: Negative Staining

EMS Catalog #22409

Negative staining is a very useful technique in electron microscopy. It allows characterization of isolated particles of morphology as bacteria, virus, protein, nanoparticles, liposomes, exosomes, etc.

Staining Protocol:

  1. On a piece of parafilm or any other hydrophobic carrier, place a drop of your solution (~ 10µl) and a UranyLess drop.
  2. Using our fine tweezers, place your sample drop on a formvar-carbon coated grid. for about 1 minute.
  3. Blot your grid using filter paper.
  4. Place your grid on the UranyLess solution for 1 minute.
  5. Blot, let it dry for 5 minutes and observe under the microscope.


Technical Tips:

  • If the staining is too intense, wash with room temperature water for 1 minute.

Product Information

UranyLess, Substitute for Uranyl Acetate