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Electron Microscopy Sciences

Technical Data Sheets

UranyLess – Frequently Asked Questions

EMS Catalog #22409

What is UranyLess made from?

UranyLess is a solution ready for use, a mix of lanthanides (rare-earths).

How is UranyLess sold?

In an aqueous solution (water).

What is it's shelf life?

One year.

What are the storage conditions for UranyLess?

Store it at room temperature away from direct sunlight.

Does it need to be diluted?

No, it is sold ready for use.

What is its pH?

UranyLess pH is 6.8-7.

How to stain with UranyLess?

Simply drop UranyLess on your grid, and wait a minute. Dry, then contrast with lead citrate according to Reynolds method.

How do I avoid precipitates (dirt) on the grids?

Precipitates form when lead citrate is washed with cold water which is loaded with CO2. Use water that has never been at low temperature and never store lead citrate at low temperature.

Is it the same protocol for every kind of tissue (animal, plant, marine)?

Yes it is - a double stain of UranyLess plus Lead citrate.

Does it adjust to every kind of resin?

Yes, it operates with every kind of resin (Epon, Araldite, Spurr).

Can it be used on negative staining?

Yes, it can be used on negative staining.

Can it be used for bloc contrast?

Some tests are in progress.

Is it adapted to a cryo use?

No, because it is prepared in water. However, we are currently developing many formulations of UranyLess, including ethyl UranyLess and acetone UranyLess, the latter being the best-adapted to cryogenic use.

Is it efficient on marine material?

Yes.

UranyLess

How is UranyLess packaged?

We sell UranyLess in an airless 30ml bottle and also in a brown 200ml bottle.

How does an airless bottle operate?

Its use is very simple; simply push on the head of the bottle to get a drop. When you release, the bottle back pump actuator lifts up. It prevents any air inlet in the bottle.

UranyLessWhat is the advantage of an airless bottle?

It is a bottle in which air never enters. Some products, such as lead citrate, are atmospheric CO2 sensitive. Thanks to this system, those products have a longer shelf life. It also allows the product to be deposited drop by drop, quickly, cleanly and in any position.

Can it be used with automated staining equipment?

Yes, the 200ml bottle is available for use with automated staining equipment. When using UranyLess for automated staining, do not wash longer than 10 minutes or you run the risk of losing all contrast.

Product Information

UranyLess, Substitute for Uranyl Acetate