French Flag   chineseFlag   japaneseFlag   germanFlag   SpanishFlag   argentinaFlag   mexicoFlag   russianFlag

View Cart || Request a 2017 Catalog

Electron Microscopy Sciences

Technical Data Sheets

EMS Xylene Substitute

EMS Catalog #23410-01 & 23410-04

General:

This EMS Xylene Substitute solution is specially designed for use in processing and staining histological/cytological specimens. It is essentially odorless with low toxicity, less irritating to the skin than xylene- and d-limonene-based clearants. It can be substituted for xylene and can be used with almost all tissue processors and automated slide stainers.

Clearing Procedure:

Most specimens may be processed with the same procedure used with xylene. If deparafinization is not complete, processing time may be increased.

Drying Time:

While EMS Xylene Substitute will dry faster then D-limonene products, it will generally take longer than xylene. To accelerate this process, it is recommended that slides be allowed to stand on edge for 2-5 minutes to allow the solution to drain off. To insure that all residual EMS Xylene Substitute is removed, tap edge of slide prior to coverslipping.

Note: Any EMS Xylene Substitute remains on the slide after coverslipping will dry completely without leaving any residue.

Heat Application:

Do not use EMS Xylene Substitute with heat applications.

Mounting Media & EMS Xylene-Subs Compatibility:

Use this EMS Xylene Substitute with a mounting medium that is compatible with xylene. The table below indicates compatibility of EMS Xylene-Subtitute with available mounting media. Failure to use a compatible mounting media may result in clouding of the specimens.

Precaution:

EMS Xylene Substitute is a flammable liquid and an aspiration hazard. May cause eyes, skin and respiratory tract irritation.

Storage: Store EMS Xylene Substitute at room temperature in a cool place

Limitations:

In extremely humid environments, EMS Xylene-Substitute may absorb moisture from the air. More frequent changes may be necessary.

Clearing Solvents:

Mounting Media
EMS XyleneSubs
D-limonene
Xylenes
PROTOCOL SecureMount
C
C
C
Richard Allen
C
C
C
Coverbrand
-
-
C
Fisher Permount
C
C
C
Refrax
C
C
C
Shandon Xylene Sub
C
C
C
DPX
C
C
C
PROTOCOL X&T
C
C
C
Cytoseal
NR
NR
C
Accumount 60
NR
NR
C
Acrytol
NR
NR
C
Clearium
NR
NR
C
Eukitt
-
-
C
Flo-Texx
-
-
C
Histomount
-
-
C
Hypermount
-
-
C
Permaslip
-
-
C
Pro-Texx
NR
NR
C
Shandon Mounting
NR
NR
C
Stephens Substitute
NR
NR
C
Micromount
NR
NR
C
E-Z Mount
NR
NR
C

C = Compatible NR = Not Recommended

Online Ordering

EMS Xylene Substitute is available online from the EMS Catalog. For ordering or product information, click here.