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Electron Microscopy Sciences

Technical Data Sheets

Kinyoun's Solution

EMS Catalog #26087

Microwave Kinyoun's Method For Acid Fast Bacteria

Section

Paraffin and frozen sections, air dried smears, touch preps.

Procedure

  1. Deparaffinize sections in 2 changes of Xylene, and absolute alcohol. Air dry slides.
  2. Filter 40 ml Kinyoun's Carbol Fuchsin Solution, into a plastic Coplin jar. Place slides in the jar; cover with a loose screw cap. Heat in the microwave for 50 seconds.
  3. Remove the Coplin jar from the oven; rinse slides individually in 50% Alcohol. Differentiate in Acid Alcohol, 1%,until sections are faint pink. Dip a few times. Rinse in 50% alcohol, then in distilled water.
  4. Counterstain lightly with Methylene Blue Working Solution, for two seconds. To prepare working solution from Methylene Blue Stock Solution, mix:
    Methylene Blue Stock, .....10 vols
    Tap Water ...........90 vols
  5. Dehydrate clear in Xylene. Mount

Note: All the alcohol dilutions used in this method should be prepared with absolute 100% alcohol.

Results

Acid Fast Bacteria
Red
Other tissue elements
Blue
Other types of bacteria
Blue

References

Valle, Sylvia; Journal of Histology , vol. 9, No. 4, December 1986.

Online Ordering

Kinyoun's Solution is available online from the EMS Catalog. For ordering or product information, click here.