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Electron Microscopy Sciences

Technical Data Sheets

Alcoholic Congo Red Solution

EMS Catalog #26090-05, 26090-06

Staining Protocol

  1. Deparaffinize and hydrate to distilled water.
  2. Stain in filtered Congo Red, 1% Aqueous for 1 hour.

Note: If using a microwave, stain in Congo Red, 1% Aqueous for 25 seconds. The hot stain jar is removed from the oven and should sit at room temperature for 1 minute.

  1. Rinse in distilled water.
  2. Quickly differentiate in Alkaline Alcohol.
  3. Wash in running tap water for approximately 5 minutes.
  4. Counterstain in Mayer's Acid Hemalum for approximately 5 minutes.

Note: If using a microwave, oven-stain for 30 seconds.

  1. Wash in running tap water for 15 minutes.
  2. Dehydrate and clear through 95% ethyl (absolute alcohol changes each), 2 minutes each.
  3. Mount using resinous medium.

Stain Results

Amyloids Pink Red
Nuclei Blue
Elastic Tissue Light Red

Product Information

Alcoholic Congo Red Solution