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Electron Microscopy Sciences

Technical Data Sheets

Truant's Fluorescent Method For Acid Fast Organisms

EMS Catalog #26102

Fixation

10% Buffered Neutral Formalin

Section

Paraffin, @ 6 microns

Staining Procedure

Use control slide
  1. Deparaffinize and hydrate to distilled water.
  2. Weigert's Iron Hematoxylin solution for 10 minutes. Prepare working Weigert's Hematoxylin by mixing 1 vol. of Weigert's Hematoxylin A, with 1 vol. of Weigert's Hematoxylin B. Wash in running water for 10 minutes.
  3. Auramine-Rhodamine Solution, @ 60 °C for 10 minutes, or 37 °C for 15 minutes, or 25 °C for 20 minutes. Wash in tap water.
  4. Remove excess stain with Acid Alcohol, 1%, for 2-3 minutes. Wash in running water. Rinse in distilled water.
  5. Dehydrate in 95% alcohol, absolute alcohol, and clear in Xylene, two changes each.
  6. Mount with Permount.

Results

Acid Fast flourescent reddish yellow
Artifacts flourescent yellow

References

Traunt, J.P. Henry Ford Hospital Med. Bull., 10:287, 1962.

AFIP Manual of Histological Staining Methods, 3 rd ed. , Ed. L. Luna: New York : McGraw Hill
Publications, c. 1968, p. 219.

Clark, G.: Staining Procedures, Williams and Wilkins Co., Baltimore , 3 rd Ed., c. 1973, p. 225.

Online Ordering

Weigert’s Iron Hematoxylin Solution A, Weigert’s Iron Hematoxylin Solution B, Auramine-Rhodamine Staining Solution and Acid Alcohol 1% for Truant's Fluorescent Method for Acid Fast Organisms are available online from the EMS Catalog. For ordering or product information, click here.