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Electron Microscopy Sciences

Technical Data Sheets

Taylor's Method for Bacteria

EMS Catalog #: 26108-Series

Fixation:

10% Buffered Neutral Formalin (#15740)

Section:

Paraffin @ 6 microns

Staining Procedure:

Use Control Slide

  1. Deparaffinize and hydrate to distilled water.
  2. Harris Hematoxylin, (#26108-01) for 5-10 minutes.  Wash in running distilled water 1 minute.
  3. Differentiate in Acid Alcohol, (#26108-06).  Wash in running water 3 minutes.
  4. Wash in Lithium Carbonate Solution, (#26108-05) to intensify blue  ***From this point carry only one slide at a time.
  5. Wash in running water, 5 minutes.  Hucker's Crystal Violet, (#26108-02) two minutes.  Wash quickly in water.
  6. Mordant in Gram's Iodine, (#26108-04) 1 minute.  Wash in water.  Blot with filter paper moistened with water, but do not allow to dry.
  7. Decolorize in Acetone:Alcohol, (#26108-07) until no more blue comes off.  Blot with filter paper moistened with Acetone:Alcohol, but do not allow to dry.
  8. Basic Fuchsin Working, (#26108-3A) or (5.0ml Basic Fuchsin Stock (#26108-03) and 60.0ml Distilled water) for 3 minutes.  Wash in water.  Blot with filter paper moistened with water, but do not allow to dry.
  9. Dip in Acetone, (#26108-08) until section begins to decolorize.
  10. Differentiate in Picric Acid-Acetone, (A-108-9) until section becomes reddish-brown-yellow (15 seconds).
  11. Pass quickly through Acetone-Xylene, 1:2, (#26108-10), then Acetone-Xylene, 1:3 (#26108-11).
  12. Clear in Xylene, (#23400) two changes.  Mount.

Results:

Gram-Positive Organisms blue to blue-black
Gram-Negative Organisms bright red
Nuclei brownish red
Erythrocytes red to yellow-green
Necrotic Tissue yellow-green
Cytoplasm yellow
Connective Tissue red

References:

AFIP Manual of Histological Staining Methods, 3rd ed., Ed. L. Luna:  New York:  McGraw Hill Publications, c. 1968, p. 226.

Clark, G.:  Staining Procedures, Williams and Wilkins Company, Baltimore, 3rd Ed., c. 1973, p. 320

Taylor, R.D., Amer. J. Clin. Path., 46:472 (1966).

Online Ordering

Taylor's Method for Bacteria is available online from the EMS Catalog. For ordering or product information, click here.