French Flag   chineseFlag   japaneseFlag   germanFlag   SpanishFlag   argentinaFlag   mexicoFlag   russianFlag

View Cart || Request a 2017 Catalog

Electron Microscopy Sciences

Technical Data Sheets

Giemsa For H-Pylori And Mast Cells (microwave )

EMS Catalog #26110

Fixation

10% Buffered Neutral Formalin

Sections

3 microns

Procedure

  1. Deparaffinize and hydrate to distilled water.
  2. Place promptly in Methanol for 2 minutes.
  3. Preheat Working Geimsa solution in microwave on high for 45 seconds. Filter the hot solution into a coplin jar containing the slides. Stain for 2 minutes.
    To prepare working solution mix:

    •  7.0 ml Geimsa Stock
    •  38 ml Distilled water
    •  5 ml Methanol
    Prepare fresh- do not reuse!!
  4. Rinse quickly in distilled water
  5. Dip one slide at a time in Acetic Acid, 1% .
  6. Rinse quickly in distilled water.
  7. Rinse in 3 changes of Methanol.
  8. Clear in Xylene and mount.

Stain Results

Red Blood Cells Pink
Nuclei Blue
H-Pylori, fungi and other bacteria Blue
Mast Cells Purple
Rickettsia Purple

NOTE: The use of methanol to dehydrate stained sections prior to mounting seems to provide lighter staining of surrounding tissue, offering better contrast with stained organisms.

References

Helicobacter pylori in peptic ulcer disease, NIH Consensus Statement Online, January 1994, 12 (1): 1:23 . Luna l AFIP 3 rd ed. McGraw Hill, 236.

Fail, Rena , AS , HT (ASCP) Medical University of South Carolina , Histologic . Vol. XXXIV, No. 2, Nov. 2001. P 32.

Online Ordering

Methanol, Giemsa Stock Solution, and Acetic Acid, 1% for Giemsa For H-Pylori And Mast Cells are available online from the EMS Catalog. For ordering or product information, click here.