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Electron Microscopy Sciences

Technical Data Sheets

May Grunwald Giemsa For H-Pylori

EMS Catalog #26114

Fixation

10% Buffered Neutral Formalin

Sections

3 microns

Procedure

  1. Deparaffinize and hydrate to distilled water.
  2. Place promptly in methanol (methyl alcohol), two changes, three times each.
  3. Stain in Jenner's Working solution for 6 minutes.

    To prepare working solution mix:
    •  1 part Jenners Stain
    •  1 part Distilled water
    Prepare fresh!
  4. Stain in Working Geimsa solution for 10 minutes.

    To prepare working solution mix:
    •  2.4 ml Geimsa Stock
    •  50 ml Distilled water
    Prepare fresh-do not reuse!
  5. Differentiate in Acetic Acid, 0.1% (A-114-3) one slide at a time, gently agitate, checking often under microscope until defined.
    DO NOT OVER DIFFERENTIATE!
  6. Dehydrate quickly in tert-Butyl Alcohol to Xylene
  7. Mount.

Stain Results

H-pylori Blue
Background Light purple

 

References

Carson, F. Histotechnology: A Self-Instructional Text . 1980-109.

Online Ordering

Jenner’s Stain Stock Solution, Giemsa Stock Solution, and Acetic Acid, 0.1% Solution for May Grunwald Giemsa For H-Pylori are available online from the EMS Catalog. For ordering or product information, click here.