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Electron Microscopy Sciences

Technical Data Sheets

Giemsa Thin Film Stain

EMS Catalog #: 26153-Series

Fixation:

Streak a drop of blood, leukocytes, rickettsiae culture, pus or other exudates over a sterile glass slide by means of another slide or cover glass. Fix film in Methyl Alcohol for 2-3 minutes.

Staining:

  1. Place in Ethyl or Methyl Alcohol for 5 – 7 minutes. Air dry.
  2. Stain in the Giemsa Thin Film Staining solution (#26153-01)*. Because different cells accept the stain at varying rates, prepare 3 – 4 sample slides and stain from15 – 20 minutes. Some membranous structures (Trypanosomes) may require 1 – 2 hour periods.
    1. To prepare Giemsa Thin Film Staining solution from Giemsa Stock solution (#26153-03), mix before use:
      Giemsa Stock Solution (#26153-03) - 1 ml
      Phosphate Buffer (#26153-02) -50 ml
  3. Wash in distilled water and air dry.

Stain Results:

Nuclei, additional Leukocyte features are similar to Wright Stain results. Reddish Purple
Plasmodic cytoplasm Blue
Chromatin Red

References:

Clark, G., Staining Procedures, Williams and Wilkins Co. Baltimore, 3rd, c. 1973, p. 125

Online Ordering

Giemsa Thin Film Stain is available online from the EMS Catalog. For ordering or product information, click here.