Giemsa Thin Film Stain
EMS Catalog #: 26153-Series
Streak a drop of blood, leukocytes, rickettsiae culture, pus or other exudates over a sterile glass slide by means of another slide or cover glass. Fix film in Methyl Alcohol for 2-3 minutes.
- Place in Ethyl or Methyl Alcohol for 5 – 7 minutes. Air dry.
- Stain in the Giemsa Thin Film Staining solution (#26153-01)*. Because different cells accept the stain at varying rates, prepare 3 – 4 sample slides and stain from15 – 20 minutes. Some membranous structures (Trypanosomes) may require 1 – 2 hour periods.
- Wash in distilled water and air dry.
|Nuclei, additional Leukocyte features are similar to Wright Stain results.||Reddish Purple|
Clark, G., Staining Procedures, Williams and Wilkins Co. Baltimore, 3rd, c. 1973, p. 125