French Flag   chineseFlag   japaneseFlag   germanFlag   SpanishFlag   argentinaFlag   mexicoFlag   russianFlag

View Cart || Request a 2017 Catalog

Electron Microscopy Sciences

Technical Data Sheets

Giemsa Thick Film Stain

EMS Catalog #: 26154-Series

Fixation:

None. Films are prepared by spreading 3 – 5 drops of blood on a scrupulously clean slide to form a circle of about 15mm diameter: the films are air dried by keeping in a horizontal position for 18 – 24 hours at room temperature, protected from dust and insects.

Staining:

  1. Stain 40 -120 minutes in Giemsa Thick Film Staining Solution (#26154-01). *
    1. To prepare Giemsa  Thick Film Staining Solution from  Giemsa Stock Solution (#26154-03)., mix before use:
                  Giemsa Stock Solution (#26154-03) - 1 ml
                  Phosphate Buffer (#26154-02) - 2 ml
                  Distilled Water - 47 ml
  2. Wash 5-10 minutes in distilled water or Phosphate Buffer (#26154-02)
  3. Dry in the Air and examine.

Stain Results:

Malarial Parasites Clear Red Chromatin
Cytoplasm Clear Blue
Red Corpuscles Not seen due to lacking of hemoglobin during drying

References:

Clark, G., Staining Procedures, Williams and Wilkins Co. Baltimore, 3rd, c. 1973, p. 126

Lillie, R. D. Histologic Technic and Practical Histochemistry, 3rd ed., Mcgraw-Hill, New York, 1965.

Barber, M.A., and Komp, H.W., Pub. Health Rep., 44:2330-41, 1929

Online Ordering

Giemsa Thick Film Stain is available online from the EMS Catalog. For ordering or product information, click here.