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Electron Microscopy Sciences

Technical Data Sheets

Prussian Blue Method for Hemosiderin

EMS Catalog #: 26170-Series

Fixation:

10% Buffered Neutral Formalin (#15740-01).

Sections:

Paraffin

Staining:

  1. Deparaffinize and hydrate to Distilled Water.                            
  2. Place sections in working staining solution for 30 minutes) at 60°C or for one hour  at room temperature. Rinse in distilled water.
    1. Prepare working stain by mixing equal volumes of Potassium Ferrocyanide, 2% (#26170-01) and Hydrochloric Acid, 2% (#26170-02). Prepare fresh just before use.
  3. Counterstain in Safranin O, 0.2% (#26170-03) for 2 minutes. Wash in Acetic Acid 1% (#26170-04)
  4. Dehydrate in 95% Alcohol, Absolute Alcohol, clear in Xylene , and mount in synthetic resin, such as DPX (#13510)

Stain Results:

Hemosiderin Blue or Green
Nuclei Red
Background Pink

References:

Perls, M., Virchow’s Arch. F. Path. Anat., 39:42-48, 1867.

Highman, B., Arch. Path., 33:937-938, 1942.

Lillie, R.D., Histopathologic Technic and Pratical Histochemistry, 3rd. ed., McGraw Hill, N.Y., 1965

Clark, G., (ed.), Staining Procedures, 3rd ed., Williams & Wilkins, Baltimore, p. 157, c. 1973

Online Ordering

Prussian Blue Method for Hemosiderin is available online from the EMS Catalog. For ordering or product information, click here.