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Electron Microscopy Sciences

Technical Data Sheets

3-Amino-9-Ethylcarbazole Method For Leukocyte Eroxidase

EMS Catalog #26173

Fixation

Formalin-Acetone Fixative

Procedure

  1. Fix thin blood films in Formalin Acetone Fixative for 15 seconds.
  2. Rinse gently in distilled water.
  3. Stain in 3-Amino-9-Ethylcarbazole staining solution for 2 ½ minutes.
  4. Wash in running tap water.
  5. Stain in Mayer's Hematoxylin for 8 minutes.
  6. Wash in tap water, air dry, and mount in Glycerine Jelly.

Stain Results

Sites of peroxidase activity Red-Brown granules
Nuclei Blue

References

Willams, W. J., Beutler, E., Erslev, A.J. and Rundles, R.W. Hematology , 2 nd ed.
McGraw-Hill , NY , c 1977, pp1627-1628.

Online Ordering

Formalin-Acetone Fixative, 3-Amino-9-Ethylcarbazole Staining Solution, and Mayer Hematoxylin for 3-Amino-9-Ethylcarbazole Method are available online from the EMS Catalog. For ordering or product information, click here.