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Electron Microscopy Sciences

Technical Data Sheets

Maximow's Method for Bone Marrow

EMS Catalog #: 26252-Series

Fixation:

Zenker’s / Formalin

Sections:

Paraffin at 6 microns

Staining Procedures:              

  1. Deparaffinize and hydrate to distilled water.
  2. Mayer’s Hematoxylin (#26252-01) for 15 minutes. Wash in tepid tap water for 15 minutes.
  3. Stain in working solution overnight. Prepare working solution fresh each time as follows:
    1. Eosin Y, 0.1%, Aqueous (#26252-02) - 10 ml
      Triple distilled water or Phosphate Buffer (#26252-04) - 100 ml
      Azur II, 0.1%, Aqueous (#26252-03) - 10 ml
      Glacial Acetic Acid - 2 drops
  4. Differentiate in 95% alcohol until blue ceases to come out into alcohol and erythrocytes and collagen are pink.
  5. Dehydrate in absolute alcohol, clear in xylene, two changes each.
  6. Mount with Permount (#17986-01).

Stain Results:

Nuclei Blue
Basophile leucocyte, mast cell Purple to Violet
Cartilage Purple
Erythrocytes, eosinophile granules Pink
Cytoplasm Blue to Pink

References:

AFIP, Manual of Histologic Staining Methods, 3rd edition, Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 120

Mallory, F.B., Pathological Technique, New York, Hafner Publishing Co., 1961, p. 196.

Online Ordering

Maximow's Method for Bone Marrow is available online from the EMS Catalog. For ordering or product information, click here.