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Electron Microscopy Sciences

Technical Data Sheets

Lacquers's Method for Alcoholic Hyalin

EMS Catalog #: 26306-Series

Fixation:

10% Buffered Neutral Formalin or Zenker’s

Sections:

Cut Paraffin, 6 microns.

Staining Procedures:

  1. Deparaffinize and hydrate to distilled water.
  2. Stain in Mayer Hematoxylin (#26306-01) for 15 minutes. Wash in running water for 15 minutes.
  3. Flood slides with Acid Fuchsin -:Aniline  Solution (#26306-02). Shake bottle well before using, solution will separate upon standing, and heat gently until aniline fumes escapes, view against a dark background. Let stand for 15 minutes. Wash in water, 3 dips.
  4. Differentiate in Alcoholic Picric Acid (#26306-05) until only hyaline and red cells remain red, and collagen is faint gray or unstained. Wash in running water for 3 minutes.
  5. Mordant in 1% Phosphomolybdic Acid (#26306-04) for 4 to 18 hours.
  6. Counterstain in Light Green Solution (#26306-03) for 1 hour. Wash in running water for 1 minute.
  7. Fibers are seen as distinct fibrils.
  8. Dehydrate in 95% alcohol, absolute alcohol, and clear in xylene, two changes each.
  9. Mount with Permount (#17986-01).

Stain Results:

Mallory Bodies Bright Red
Erythrocytes Red
Cystoplasm Pale Brown
Bile Pigment Green
Protinaceous material seen occasionally in some liver cells Red
Hemosiderin and lipofichsin Unstained

References:

Lacquer, G.I., Amer. J. Clin. Path., 20:689-690, 1950.

Luna, L.G. (ed), Manual of Histologic Staining Methods of the AFIP, 3rd edition, McGraw-Hill, NY, c 1968

Lillies, R.D. Fullmer, H.M., Histopathologic Technic and Pratical Histochemistry 4th ed., McGraw-Hill, N.Y. 1976, pp 670-671.

Clark, G., (ed). Staining Procedures, 3rd edition, Williams & Wilkins, Baltimore, p. 52, c.1973.

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Lacquers's Method for Alcoholic Hyalin