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Electron Microscopy Sciences

Technical Data Sheets

Mayer's Mucihematein, Modified

EMS Catalog #: 26321-Series    

Fixation:

Absolute Alcohol (Cat. #15055)  4 – 8 hours.

Sections:

Paraffin or Celloidin.

Staining Procedures:

  1. Deparaffinize and hydrate to distilled water.
  2. Place on staining rack and rinse several times with distilled water.
  3. Place 2 ml of Mayer Mucihematein, Modified (#26321-01) on each slide. Allow to remain for five minutes. Drain off stain and wash well with distilled water three times for 5 minutes each.
  4. Dehydrate in two chages of 95% alcohol, two changes of absolute alcohol, Absolute Alcohol-Xylene (50:50), and two changes of Xylene (#23400).
  5. Mount with Permount (#17986-01) or DPX (#13510).

Stain Results:

Mucin Deep Violet
Cell Nuclei Pale Gray-Blue
Connective Tissue Pale Gray to Colorless

References:

Laskey, Alice: Stain Techn., 25:33 (1950).

Clark G.: Staining Procedures, William & Wilkins Company, Baltimore, 3rd Ed., c. 1973, p. 47

Online Ordering

Mayer's Mucihematein, Modified is available online from the EMS Catalog. For ordering or product information, click here.